-frag_weight_aligned 0.1
-beta # this flag enables the latest rosetta score function 
-in:file:fasta ../KTSC.fas.fasta 
-in:file:native ../4RGIA.pdb
-parser:protocol hyb.xml # rosetta script (see below) 
-relax:minimize_bond_angles
-relax:jump_move true
-relax::dualspace
-default_max_cycles 200
-relax:min_type lbfgs_armijo_nonmonotone
-hybridize:stage1_probability 1.0
-hybridize:stage1_4_cycles 400
-nstruct 1