GREMLIN Database
PF03798 - (128-335) Ceramide synthase 2
UniProt: Q96G23 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 203 (202)
Sequences: 1336 (836)
Seq/√Len: 58.8
META: 0.279

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
53_Y93_S5.6211.00
33_M161_Y3.4291.00
20_G52_Y3.4011.00
143_I190_A3.1501.00
158_T176_N3.0611.00
57_L87_I3.0041.00
35_K38_E2.9571.00
188_F192_L2.7591.00
37_W157_C2.6601.00
128_K132_N2.6571.00
53_Y102_G2.6371.00
57_L90_I2.5471.00
155_L180_G2.5461.00
150_L155_L2.4131.00
77_K80_I2.4101.00
138_F193_I2.4101.00
139_A197_A2.3251.00
78_E188_F2.1951.00
144_I148_V2.1421.00
109_H186_H2.1241.00
182_L186_H2.1191.00
9_R117_E2.0501.00
147_L183_Q1.9561.00
157_C161_Y1.8301.00
118_S134_I1.7970.99
6_A9_R1.7560.99
85_A181_V1.7350.99
60_Y70_D1.7220.99
196_M200_F1.7050.99
28_P35_K1.6990.99
56_E60_Y1.6820.99
111_S145_T1.6750.99
141_V145_T1.6690.99
64_L87_I1.6450.99
12_F56_E1.6290.99
139_A142_F1.6260.99
51_W55_I1.6030.99
5_E73_R1.6030.99
117_E142_F1.5900.99
198_H202_T1.5660.98
9_R13_Y1.5660.98
36_V104_L1.5610.98
93_S102_G1.5590.98
13_Y59_F1.5390.98
14_L114_Y1.5290.98
143_I194_L1.5270.98
34_K161_Y1.5170.98
122_F134_I1.5060.98
194_L198_H1.5030.98
32_D35_K1.4920.98
13_Y60_Y1.4770.98
110_D113_D1.4720.98
10_F14_L1.4660.98
161_Y164_E1.4240.97
37_W161_Y1.4040.97
6_A117_E1.4000.97
109_H117_E1.3820.97
175_F178_M1.3650.96
21_M107_A1.3460.96
70_D124_Y1.3140.95
101_A154_I1.3070.95
49_Q103_T1.2990.95
180_G184_L1.2860.95
46_I49_Q1.2530.94
135_F197_A1.2530.94
116_L138_F1.2420.94
2_K6_A1.2120.93
16_A55_I1.2090.93
10_F118_S1.1870.92
33_M36_V1.1870.92
19_A48_S1.1840.92
129_N133_N1.1760.92
27_K46_I1.1670.92
61_W87_I1.1560.91
12_F16_A1.1520.91
190_A194_L1.1460.91
155_L158_T1.1400.91
120_K124_Y1.1340.90
81_I185_L1.1300.90
84_V88_I1.1270.90
59_F63_L1.1250.90
127_W130_T1.1250.90
74_K124_Y1.1050.89
124_Y138_F1.0920.89
63_L70_D1.0870.88
159_L173_Y1.0660.87
162_P166_Y1.0520.87
112_S145_T1.0510.87
119_A131_C1.0510.87
147_L151_P1.0490.87
120_K135_F1.0430.86
112_S141_V1.0340.86
39_G42_I1.0290.86
31_Y36_V1.0180.85
10_F121_M1.0160.85
67_I80_I1.0110.84
22_A26_D1.0110.84
101_A175_F1.0010.84
167_P170_F0.9960.84
80_I84_V0.9890.83
153_W156_H0.9870.83
54_M58_S0.9850.83
18_I22_A0.9840.83
7_S11_T0.9820.83
139_A143_I0.9500.81
119_A134_I0.9360.80
83_H86_T0.9340.79
28_P39_G0.9320.79
109_H113_D0.9300.79
109_H147_L0.9270.79
60_Y124_Y0.9220.79
162_P168_A0.9210.78
13_Y107_A0.9170.78
148_V151_P0.9150.78
148_V152_F0.9120.78
2_K109_H0.9070.77
104_L154_I0.9040.77
169_F173_Y0.8990.77
22_A48_S0.8970.77
133_N137_V0.8820.75
57_L91_S0.8810.75
136_I140_I0.8810.75
152_F156_H0.8800.75
123_N142_F0.8670.74
16_A59_F0.8610.74
157_C162_P0.8610.74
193_I197_A0.8530.73
159_L176_N0.8500.73
56_E142_F0.8460.72
55_I58_S0.8460.72
7_S121_M0.8440.72
119_A135_F0.8390.72
58_S74_K0.8370.72
58_S154_I0.8340.71
9_R63_L0.8320.71
75_D82_H0.8270.71
33_M104_L0.8260.71
48_S105_I0.8120.69
26_D95_F0.8110.69
193_I196_M0.8110.69
170_F174_F0.8060.69
36_V70_D0.8060.69
172_Y176_N0.8020.68
111_S141_V0.7990.68
62_S72_K0.7980.68
177_S198_H0.7930.67
122_F127_W0.7910.67
155_L181_V0.7860.67
85_A92_F0.7840.66
19_A23_V0.7840.66
163_L172_Y0.7800.66
41_P52_Y0.7770.66
181_V195_R0.7760.66
2_K12_F0.7640.64
70_D147_L0.7590.64
3_F7_S0.7590.64
9_R12_F0.7550.64
78_E105_I0.7470.63
105_I181_V0.7390.62
112_S117_E0.7380.62
71_V117_E0.7380.62
78_E185_L0.7370.62
192_L196_M0.7320.61
1_K121_M0.7280.61
84_V136_I0.7250.60
72_K76_F0.7250.60
191_Y194_L0.7230.60
106_M150_L0.7230.60
19_A22_A0.7230.60
105_I113_D0.7200.60
156_H160_V0.7150.59
82_H86_T0.7140.59
61_W191_Y0.7090.59
50_Y54_M0.7090.59
44_S47_P0.7070.58
171_G174_F0.7030.58
50_Y94_W0.6980.58
63_L196_M0.6970.57
23_V26_D0.6970.57
44_S140_I0.6930.57
8_W117_E0.6880.56
81_I181_V0.6880.56
42_I97_N0.6860.56
136_I201_I0.6840.56
75_D83_H0.6840.56
147_L190_A0.6840.56
15_I25_V0.6840.56
144_I149_I0.6830.56
66_S139_A0.6830.56
68_A76_F0.6790.55
197_A201_I0.6760.55
171_G176_N0.6730.55
115_L137_V0.6690.54
45_T49_Q0.6680.54
122_F130_T0.6660.54
36_V61_W0.6610.53
59_F64_L0.6610.53
128_K131_C0.6610.53
172_Y175_F0.6570.53
79_Q175_F0.6560.53
111_S129_N0.6560.53
191_Y195_R0.6560.53
8_W124_Y0.6550.53
12_F24_I0.6510.52
85_A185_L0.6460.52
130_T134_I0.6450.52
73_R124_Y0.6450.52
40_Y92_F0.6430.51
60_Y186_H0.6400.51
2_K117_E0.6370.51
127_W177_S0.6370.51
18_I26_D0.6340.50
74_K120_K0.6340.50
98_Y166_Y0.6290.50
114_Y187_I0.6240.49
102_G113_D0.6220.49
108_L112_S0.6200.49
92_F174_F0.6200.49
143_I148_V0.6170.49
12_F66_S0.6120.48
89_L93_S0.6110.48
124_Y135_F0.6100.48
85_A139_A0.6100.48
108_L142_F0.6090.48
118_S122_F0.6080.48
90_I106_M0.6070.47
194_L202_T0.6060.47
38_E164_E0.6060.47
146_R189_W0.6060.47
9_R109_H0.6040.47
155_L176_N0.6030.47
194_L197_A0.6010.47
12_F20_G0.5980.46
185_L188_F0.5950.46
110_D142_F0.5950.46
11_T133_N0.5910.46
34_K164_E0.5910.46
35_K158_T0.5870.45
137_V140_I0.5840.45
59_F67_I0.5820.45
79_Q86_T0.5800.44
6_A60_Y0.5780.44
139_A148_V0.5740.44
145_T149_I0.5710.43
60_Y74_K0.5710.43
170_F181_V0.5700.43
101_A157_C0.5690.43
101_A105_I0.5690.43
16_A23_V0.5690.43
2_K7_S0.5660.43
70_D74_K0.5650.43
18_I21_M0.5650.43
136_I184_L0.5650.43
1_K141_V0.5600.42
2_K160_V0.5590.42
116_L135_F0.5580.42
139_A193_I0.5570.42
133_N141_V0.5570.42
32_D40_Y0.5570.42
40_Y96_A0.5560.42
188_F191_Y0.5550.42
12_F142_F0.5540.42
19_A55_I0.5540.42
62_S73_R0.5520.41
116_L119_A0.5520.41
14_L19_A0.5520.41
7_S36_V0.5520.41
75_D86_T0.5480.41
34_K165_L0.5460.41
116_L139_A0.5460.41
94_W178_M0.5450.41
92_F175_F0.5450.41
64_L91_S0.5420.40
10_F23_V0.5410.40
50_Y70_D0.5400.40
162_P190_A0.5390.40
156_H161_Y0.5380.40
16_A19_A0.5370.40
129_N140_I0.5340.39
21_M25_V0.5340.39
60_Y63_L0.5340.39
29_W36_V0.5330.39
116_L193_I0.5330.39
68_A130_T0.5320.39
60_Y117_E0.5320.39
2_K54_M0.5310.39
61_W133_N0.5250.39
65_F69_S0.5230.38
127_W181_V0.5170.38
11_T129_N0.5130.37
38_E165_L0.5100.37
11_T28_P0.5090.37
28_P149_I0.5080.37
35_K81_I0.5080.37
182_L189_W0.5060.37
44_S55_I0.5050.36
30_F107_A0.5040.36
136_I154_I0.5040.36
129_N201_I0.5020.36
23_V103_T0.5010.36
6_A63_L0.5010.36
73_R114_Y0.5000.36
32_D99_I0.5000.36
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2qklB 1 0.4138 2.5 0.967 Contact Map
2kihA 3 0.1872 1.3 0.972 Contact Map
3bkdA 4 0.1232 1 0.975 Contact Map
1orqC 4 0.3153 0.9 0.975 Contact Map
2ljbA 3 0.1478 0.8 0.975 Contact Map
1nwdB 2 0.0493 0.8 0.976 Contact Map
3lbwA 3 0.1084 0.7 0.977 Contact Map
4y66B 1 0.2759 0.6 0.978 Contact Map
4pfyA 1 0.3251 0.6 0.978 Contact Map
1q2hA 2 0.1034 0.5 0.979 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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