GREMLIN Database
LEU3 - 3-isopropylmalate dehydrogenase
UniProt: Q9UXB2 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 336 (318)
Sequences: 6790 (3012)
Seq/√Len: 168.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
159_L192_K4.3491.00
151_V185_C3.1461.00
20_G329_V3.0851.00
152_N156_N2.8141.00
152_N191_G2.7651.00
4_R37_D2.7481.00
202_A206_N2.6981.00
23_R330_D2.6601.00
283_S302_E2.6391.00
26_A38_L2.4161.00
57_R61_D2.3441.00
32_Y300_S2.3101.00
144_E148_K2.3101.00
18_Y274_F2.2971.00
331_E334_K2.2951.00
7_V22_K2.2631.00
20_G23_R2.2611.00
41_V62_V2.1561.00
164_K196_E2.1291.00
166_T196_E2.1241.00
185_C195_F2.1111.00
283_S299_K2.1051.00
53_E57_R2.0401.00
42_E50_K2.0211.00
6_A62_V2.0151.00
272_T332_I1.9951.00
114_V157_Y1.9951.00
148_K187_E1.9821.00
276_L305_I1.9731.00
22_K40_E1.9631.00
164_K194_N1.9441.00
151_V218_V1.9431.00
64_E89_Y1.9391.00
148_K184_V1.9071.00
210_D213_R1.8841.00
267_G324_K1.8661.00
267_G326_K1.8431.00
299_K303_N1.7971.00
186_R197_E1.7971.00
47_A56_P1.7961.00
61_D65_K1.7951.00
275_L332_I1.7951.00
15_P19_E1.7871.00
13_I269_A1.7861.00
144_E187_E1.7481.00
148_K152_N1.7431.00
134_G144_E1.7421.00
329_V333_Y1.7331.00
99_K306_I1.6911.00
279_S302_E1.6801.00
168_V203_A1.6791.00
314_K331_E1.6691.00
314_K323_A1.6671.00
64_E88_M1.6441.00
184_V187_E1.6241.00
140_K220_S1.6091.00
152_N188_I1.5521.00
155_L189_L1.5341.00
330_D334_K1.5321.00
253_F285_M1.5311.00
6_A66_A1.5221.00
156_N160_M1.5191.00
102_P310_K1.5191.00
61_D64_E1.4991.00
324_K327_D1.4981.00
21_S278_V1.4941.00
116_V153_V1.4921.00
39_I65_K1.4921.00
106_S109_P1.4891.00
130_V183_S1.4851.00
60_L88_M1.4841.00
46_V50_K1.4781.00
34_L297_A1.4701.00
95_I114_V1.4701.00
208_V230_E1.4651.00
23_R333_Y1.4581.00
167_C185_C1.4531.00
38_L278_V1.4381.00
8_I43_A1.4351.00
84_K88_M1.4261.00
6_A41_V1.4261.00
283_S298_S1.4211.00
25_I36_I1.3941.00
22_K26_A1.3891.00
162_R215_D1.3891.00
140_K177_T1.3871.00
39_I66_A1.3831.00
27_K333_Y1.3811.00
268_I317_E1.3561.00
267_G325_L1.3511.00
9_P40_E1.3501.00
115_I228_S1.3401.00
42_E51_Y1.3281.00
103_N310_K1.3211.00
21_S70_L1.3201.00
289_S299_K1.3071.00
272_T328_M1.2851.00
323_A331_E1.2831.00
198_M206_N1.2781.00
55_L60_L1.2781.00
42_E47_A1.2771.00
204_A227_L1.2721.00
308_T331_E1.2511.00
163_R215_D1.2441.00
21_S274_F1.2391.00
39_I62_V1.2301.00
116_V157_Y1.2271.00
167_C197_E1.2271.00
156_N159_L1.2261.00
188_I191_G1.2071.00
323_A327_D1.2071.00
180_L184_V1.2061.00
22_K38_L1.1991.00
4_R39_I1.1941.00
198_M202_A1.1941.00
187_E190_K1.1901.00
282_L297_A1.1901.00
63_I69_I1.1841.00
328_M332_I1.1831.00
148_K188_I1.1811.00
151_V188_I1.1711.00
198_M214_F1.1651.00
327_D330_D1.1641.00
47_A51_Y1.1601.00
198_M203_A1.1581.00
115_I231_A1.1561.00
186_R190_K1.1531.00
303_N307_E1.1461.00
207_L217_I1.1331.00
144_E184_V1.1321.00
113_L207_L1.1251.00
183_S187_E1.1241.00
24_I301_L1.1181.00
138_A177_T1.1131.00
51_Y58_H1.1131.00
204_A230_E1.1091.00
105_Q109_P1.1051.00
110_N212_T1.0981.00
161_R215_D1.0971.00
7_V40_E1.0871.00
288_L297_A1.0841.00
6_A63_I1.0821.00
25_I278_V1.0811.00
147_S151_V1.0771.00
155_L193_V1.0761.00
92_Y248_D1.0741.00
133_T145_F1.0681.00
200_V226_I1.0671.00
135_V141_V1.0590.99
263_I269_A1.0590.99
70_L278_V1.0560.99
165_V195_F1.0500.99
136_T180_L1.0490.99
267_G327_D1.0490.99
95_I116_V1.0490.99
90_D149_R1.0430.99
159_L163_R1.0410.99
99_K112_D1.0370.99
128_E139_I1.0330.99
19_E23_R1.0290.99
171_A182_A1.0260.99
307_E311_S1.0220.99
23_R326_K1.0200.99
157_Y161_R1.0150.99
123_L140_K1.0140.99
20_G326_K1.0130.99
168_V200_V1.0100.99
23_R329_V1.0100.99
206_N213_R1.0080.99
119_N245_N1.0030.99
71_K82_V1.0000.99
49_N78_A0.9970.99
287_E297_A0.9950.99
99_K302_E0.9950.99
4_R65_K0.9930.99
56_P59_T0.9920.99
93_A150_I0.9870.99
196_E213_R0.9780.99
123_L220_S0.9770.99
209_R233_Q0.9750.99
142_T220_S0.9740.99
58_H61_D0.9740.99
272_T309_Y0.9690.99
53_E84_K0.9630.99
21_S275_L0.9620.99
47_A59_T0.9600.99
154_A165_V0.9600.99
105_Q108_Y0.9600.99
113_L231_A0.9570.99
272_T305_I0.9510.99
185_C218_V0.9470.99
7_V18_Y0.9450.99
122_D140_K0.9430.99
145_F148_K0.9390.99
263_I268_I0.9360.99
317_E322_S0.9350.99
311_S314_K0.9350.99
169_H182_A0.9340.99
165_V188_I0.9300.99
297_A300_S0.9300.99
268_I322_S0.9240.99
43_A85_L0.9230.99
299_K302_E0.9210.99
9_P19_E0.9200.99
189_L195_F0.9200.99
276_L302_E0.9190.99
155_L165_V0.9150.98
24_I333_Y0.9150.98
101_L306_I0.9130.98
206_N210_D0.9110.98
32_Y297_A0.9100.98
281_M286_Y0.9090.98
142_T147_S0.9040.98
140_K178_D0.9040.98
165_V218_V0.9010.98
255_P274_F0.9000.98
24_I275_L0.8960.98
17_L274_F0.8950.98
263_I317_E0.8920.98
49_N76_E0.8840.98
165_V216_V0.8830.98
309_Y315_L0.8800.98
103_N306_I0.8760.98
41_V66_A0.8750.98
247_G250_K0.8730.98
122_D220_S0.8720.98
279_S283_S0.8700.98
58_H62_V0.8670.98
320_G323_A0.8650.98
41_V59_T0.8580.98
167_C195_F0.8570.98
178_D223_Y0.8560.98
20_G267_G0.8560.98
129_F137_I0.8550.98
95_I153_V0.8550.98
307_E310_K0.8540.98
43_A81_V0.8490.98
7_V38_L0.8450.97
20_G330_D0.8410.97
62_V66_A0.8410.97
168_V217_I0.8400.97
278_V281_M0.8380.97
262_D317_E0.8350.97
304_A308_T0.8320.97
98_A231_A0.8310.97
169_H197_E0.8310.97
135_V143_T0.8300.97
93_A153_V0.8280.97
159_L193_V0.8250.97
275_L305_I0.8200.97
308_T332_I0.8200.97
43_A71_K0.8170.97
34_L288_L0.8150.97
68_M285_M0.8140.97
244_A253_F0.8130.97
73_P257_H0.8110.97
102_P306_I0.8030.97
165_V189_L0.8020.97
286_Y289_S0.8010.97
171_A199_Y0.7980.96
45_D76_E0.7960.96
323_A328_M0.7940.96
148_K191_G0.7930.96
317_E324_K0.7930.96
166_T198_M0.7920.96
165_V193_V0.7850.96
123_L222_V0.7840.96
19_E326_K0.7800.96
25_I282_L0.7800.96
48_L54_A0.7770.96
94_N256_V0.7760.96
79_M84_K0.7750.96
98_A235_A0.7740.96
21_S25_I0.7720.96
18_Y72_G0.7710.96
36_I278_V0.7690.96
90_D120_T0.7690.96
168_V227_L0.7680.96
17_L72_G0.7680.96
154_A218_V0.7650.95
316_T328_M0.7610.95
278_V282_L0.7580.95
266_K317_E0.7570.95
187_E191_G0.7560.95
140_K222_V0.7520.95
42_E46_V0.7510.95
303_N306_I0.7500.95
8_I63_I0.7490.95
91_M252_M0.7480.95
16_E265_G0.7480.95
242_P280_M0.7460.95
151_V195_F0.7440.95
184_V188_I0.7440.95
200_V204_A0.7430.95
60_L64_E0.7410.95
136_T177_T0.7410.95
70_L274_F0.7410.95
156_N191_G0.7360.94
144_E180_L0.7330.94
207_L231_A0.7330.94
186_R195_F0.7300.94
242_P276_L0.7280.94
62_V65_K0.7250.94
121_E146_A0.7230.94
64_E250_K0.7180.94
130_V136_T0.7140.94
45_D49_N0.7130.93
128_E137_I0.7100.93
155_L195_F0.7100.93
200_V220_S0.7090.93
330_D333_Y0.7090.93
112_D215_D0.7070.93
19_E40_E0.7040.93
260_A263_I0.7030.93
45_D75_G0.7020.93
41_V58_H0.7020.93
153_V157_Y0.7020.93
20_G333_Y0.7020.93
73_P274_F0.7020.93
113_L234_I0.6980.93
98_A113_L0.6970.93
203_A217_I0.6930.92
140_K223_Y0.6920.92
115_I227_L0.6920.92
116_V150_I0.6910.92
147_S184_V0.6900.92
326_K330_D0.6890.92
182_A186_R0.6880.92
75_G259_A0.6880.92
203_A214_F0.6880.92
308_T328_M0.6860.92
150_I218_V0.6860.92
326_K329_V0.6860.92
281_M285_M0.6850.92
183_S190_K0.6850.92
138_A179_G0.6810.92
76_E80_D0.6750.91
262_D269_A0.6740.91
25_I38_L0.6730.91
202_A238_L0.6720.91
312_G315_L0.6690.91
132_S135_V0.6670.91
20_G275_L0.6670.91
321_G327_D0.6660.91
186_R189_L0.6660.91
275_L301_L0.6630.91
18_Y21_S0.6630.91
34_L287_E0.6620.91
134_G183_S0.6610.91
169_H199_Y0.6610.91
5_V281_M0.6610.91
23_R26_A0.6580.90
244_A280_M0.6570.90
283_S288_L0.6500.90
57_R84_K0.6490.90
69_I252_M0.6470.90
46_V49_N0.6460.90
246_I284_R0.6460.90
167_C181_F0.6440.89
206_N209_R0.6410.89
168_V219_T0.6370.89
316_T319_V0.6340.89
189_L193_V0.6340.89
253_F284_R0.6330.89
315_L319_V0.6330.89
170_K201_D0.6310.89
308_T314_K0.6300.88
237_S273_A0.6290.88
101_L276_L0.6260.88
59_T81_V0.6240.88
18_Y22_K0.6240.88
306_I310_K0.6230.88
147_S220_S0.6220.88
55_L85_L0.6220.88
5_V36_I0.6220.88
300_S304_A0.6210.88
115_I217_I0.6210.88
298_S302_E0.6200.88
72_G274_F0.6090.87
100_S111_V0.6070.87
81_V84_K0.6070.87
242_P273_A0.6040.86
80_D84_K0.6040.86
256_V273_A0.6020.86
159_L189_L0.5980.86
134_G145_F0.5960.86
114_V216_V0.5950.85
146_A149_R0.5920.85
114_V161_R0.5900.85
153_V248_D0.5890.85
67_D249_R0.5890.85
25_I301_L0.5870.85
208_V234_I0.5840.84
90_D247_G0.5830.84
282_L301_L0.5830.84
43_A55_L0.5820.84
36_I297_A0.5810.84
269_A317_E0.5810.84
8_I41_V0.5810.84
240_L319_V0.5810.84
90_D145_F0.5800.84
53_E58_H0.5800.84
282_L298_S0.5790.84
63_I66_A0.5740.83
178_D220_S0.5740.83
158_A165_V0.5730.83
202_A213_R0.5720.83
111_V212_T0.5720.83
19_E22_K0.5700.83
163_R192_K0.5690.83
137_I141_V0.5670.83
153_V156_N0.5660.83
47_A55_L0.5660.83
160_M287_E0.5640.82
5_V38_L0.5630.82
138_A174_M0.5620.82
63_I85_L0.5610.82
135_V144_E0.5590.82
124_Y254_E0.5580.82
90_D121_E0.5560.81
156_N188_I0.5540.81
127_F139_I0.5540.81
127_F141_V0.5540.81
55_L84_K0.5530.81
275_L329_V0.5520.81
314_K328_M0.5500.81
267_G330_D0.5480.80
43_A59_T0.5470.80
130_V175_R0.5470.80
6_A39_I0.5460.80
15_P46_V0.5440.80
21_S24_I0.5430.80
288_L299_K0.5420.80
71_K76_E0.5410.80
251_S284_R0.5390.79
42_E58_H0.5370.79
250_K253_F0.5360.79
112_D162_R0.5360.79
63_I89_Y0.5360.79
237_S240_L0.5350.79
9_P22_K0.5350.79
247_G251_S0.5330.79
114_V162_R0.5330.79
92_Y150_I0.5330.79
151_V184_V0.5270.78
40_E46_V0.5270.78
228_S238_L0.5260.78
13_I17_L0.5240.77
32_Y301_L0.5230.77
322_S327_D0.5220.77
16_E267_G0.5220.77
225_D229_D0.5220.77
203_A227_L0.5210.77
302_E306_I0.5200.77
116_V154_A0.5200.77
220_S223_Y0.5160.76
18_Y70_L0.5150.76
157_Y160_M0.5150.76
315_L323_A0.5140.76
263_I266_K0.5140.76
279_S298_S0.5130.76
60_L84_K0.5130.76
155_L188_I0.5120.76
299_K306_I0.5120.76
130_V134_G0.5100.76
9_P15_P0.5090.75
155_L185_C0.5090.75
47_A50_K0.5090.75
245_N254_E0.5090.75
136_T220_S0.5080.75
246_I251_S0.5050.75
92_Y153_V0.5030.75
126_G174_M0.5030.75
300_S303_N0.5000.74
41_V56_P0.5000.74
325_L329_V0.5000.74
71_K85_L0.5000.74
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1wpwA 4 0.997 100 0.024 Contact Map
2d1cA 2 0.9673 100 0.043 Contact Map
3flkA 3 0.9881 100 0.043 Contact Map
3u1hA 2 0.9524 100 0.045 Contact Map
2y3zA 2 0.9673 100 0.046 Contact Map
2e0cA 2 0.9643 100 0.047 Contact Map
1x0lA 4 0.9554 100 0.049 Contact Map
3dmsA 2 0.9762 100 0.049 Contact Map
2d4vA 2 0.9762 100 0.051 Contact Map
1tyoA 2 0.9732 100 0.052 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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