SYP - SULSO - GREMLIN Contacts

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SYP - Proline--tRNA ligase

UniProt:	Q9UWX2	Sequences: MQITREKWSKNFSEWFDWVLREGEFYDYGRYPIKGMGVWMPYGFKLRQNIIGIIRNFLDSTGHEEVLFPLLIPEDLLRRESTHIKGFEEEVFWVTKGGSTDLDVKLALRPTSEVAITTMENLWLKSYKQLPKKYYQIVSVFRYETKATRPMIRLREITTFKEAHTVHETYDDAQKQVEEAIEIYKKIFDTLTIPYVLSERPEWDRFAGALHTYAFDTIMPDGRAMQIGTVHHLGQNFSKALDFKIQKKDGSLDYPHQTSYGISDRAVASVIAIHGDDHGPVLPPSVAPIKVVVIPIPAKNEEETQQVMKYSIEICEMFNKNNITCLTDQDTEKTPGEKFYIWEIKGVPIRLEIGPRELISGTVFIKRRDNFKSYTVRKEEAINKIKELLNEIQEDLRRKAWEDLKSRIEYSNDIEKAKKILGNNSGIVEVPWCGSNECGLKIEELTNARVLGYPIEKREVNDKCVICKMNAKTVLRIAKTY	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	481 (363)
Sequences:	8159 (3634)
Seq/√Len:	190.7

ECOLI - SYT SYP
BACSU - SYT2 SYT1 SYP
HALSA - SYP SYT
SULSO - SYP SYT

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
247_K	253_D	5.551	1.00
307_V	355_P	3.399	1.00
313_E	317_M	2.997	1.00
380_E	383_N	2.931	1.00
74_E	78_R	2.878	1.00
310_Y	313_E	2.692	1.00
57_F	60_S	2.630	1.00
313_E	361_G	2.613	1.00
315_C	319_N	2.609	1.00
249_D	253_D	2.445	1.00
362_T	375_T	2.410	1.00
57_F	186_K	2.352	1.00
292_V	342_W	2.352	1.00
374_Y	384_K	2.351	1.00
174_Q	221_D	2.346	1.00
293_V	315_C	2.338	1.00
317_M	382_I	2.332	1.00
24_E	40_M	2.316	1.00
331_T	341_I	2.247	1.00
377_R	380_E	2.242	1.00
315_C	325_C	2.238	1.00
175_K	179_E	2.237	1.00
24_E	123_W	2.232	1.00
247_K	251_S	2.225	1.00
315_C	327_T	2.221	1.00
48_Q	51_I	2.213	1.00
291_V	323_I	2.197	1.00
321_N	386_K	2.142	1.00
74_E	105_K	2.142	1.00
244_K	252_L	2.118	1.00
189_D	195_Y	2.114	1.00
172_D	175_K	2.105	1.00
362_T	377_R	2.093	1.00
55_R	65_E	2.080	1.00
49_N	324_T	2.024	1.00
66_V	119_M	1.970	1.00
182_E	186_K	1.968	1.00
95_T	105_K	1.943	1.00
355_P	359_I	1.928	1.00
309_K	313_E	1.927	1.00
167_H	172_D	1.926	1.00
383_N	387_E	1.914	1.00
52_G	56_N	1.903	1.00
312_I	316_E	1.889	1.00
46_L	267_V	1.879	1.00
185_K	189_D	1.878	1.00
317_M	379_E	1.861	1.00
27_D	40_M	1.858	1.00
290_K	324_T	1.833	1.00
80_E	121_N	1.831	1.00
235_Q	239_K	1.814	1.00
174_Q	232_H	1.801	1.00
66_V	116_I	1.771	1.00
209_A	212_T	1.745	1.00
310_Y	358_L	1.742	1.00
289_I	326_L	1.732	1.00
312_I	315_C	1.727	1.00
71_L	141_F	1.723	1.00
186_K	190_T	1.718	1.00
75_D	78_R	1.717	1.00
367_R	370_N	1.715	1.00
271_I	282_L	1.708	1.00
294_I	335_P	1.706	1.00
169_T	172_D	1.693	1.00
386_K	390_N	1.654	1.00
323_I	386_K	1.647	1.00
59_D	135_Y	1.646	1.00
384_K	388_L	1.627	1.00
356_R	359_I	1.605	1.00
42_Y	345_K	1.590	1.00
39_W	267_V	1.587	1.00
361_G	379_E	1.584	1.00
316_E	320_K	1.579	1.00
270_V	286_V	1.568	1.00
235_Q	256_H	1.560	1.00
293_V	311_S	1.557	1.00
384_K	387_E	1.548	1.00
365_I	381_A	1.545	1.00
178_E	182_E	1.544	1.00
60_S	319_N	1.543	1.00
49_N	52_G	1.537	1.00
309_K	312_I	1.504	1.00
312_I	327_T	1.484	1.00
317_M	378_K	1.468	1.00
45_K	324_T	1.452	1.00
310_Y	353_I	1.446	1.00
56_N	190_T	1.443	1.00
380_E	384_K	1.424	1.00
335_P	339_F	1.421	1.00
72_I	115_A	1.419	1.00
167_H	176_Q	1.408	1.00
18_W	22_E	1.408	1.00
53_I	190_T	1.384	1.00
57_F	61_T	1.383	1.00
232_H	256_H	1.372	1.00
294_I	350_R	1.366	1.00
296_I	335_P	1.365	1.00
382_I	386_K	1.361	1.00
356_R	360_S	1.354	1.00
185_K	195_Y	1.353	1.00
48_Q	52_G	1.341	1.00
245_I	253_D	1.337	1.00
299_K	355_P	1.334	1.00
291_V	351_L	1.320	1.00
297_P	307_V	1.313	1.00
117_T	243_F	1.309	1.00
176_Q	179_E	1.306	1.00
351_L	381_A	1.302	1.00
323_I	385_I	1.298	1.00
174_Q	178_E	1.295	1.00
317_M	321_N	1.284	1.00
202_E	206_F	1.283	1.00
63_H	133_K	1.278	1.00
48_Q	59_D	1.274	1.00
217_T	220_P	1.267	1.00
33_I	36_M	1.260	1.00
295_P	327_T	1.254	1.00
182_E	185_K	1.246	1.00
84_I	107_A	1.242	1.00
44_F	48_Q	1.236	1.00
349_I	367_R	1.236	1.00
327_T	330_D	1.233	1.00
307_V	358_L	1.230	1.00
314_I	378_K	1.230	1.00
284_P	349_I	1.221	1.00
206_F	210_L	1.212	1.00
246_Q	252_L	1.212	1.00
196_V	224_A	1.207	1.00
343_E	350_R	1.198	1.00
20_L	25_F	1.191	1.00
42_Y	289_I	1.180	1.00
45_K	49_N	1.179	1.00
284_P	388_L	1.176	1.00
71_L	94_V	1.173	1.00
78_R	85_K	1.170	1.00
71_L	108_L	1.168	1.00
349_I	385_I	1.168	1.00
365_I	376_V	1.160	1.00
86_G	236_N	1.160	1.00
334_T	337_E	1.155	1.00
47_R	159_T	1.155	1.00
364_F	375_T	1.152	1.00
74_E	84_I	1.150	1.00
281_V	346_G	1.146	1.00
11_N	14_E	1.144	1.00
311_S	327_T	1.136	1.00
349_I	365_I	1.136	1.00
298_A	308_M	1.121	1.00
292_V	347_V	1.120	1.00
173_A	232_H	1.116	1.00
342_W	347_V	1.115	1.00
133_K	176_Q	1.111	1.00
365_I	384_K	1.109	1.00
284_P	290_K	1.108	1.00
342_W	350_R	1.103	1.00
281_V	369_D	1.100	1.00
61_T	183_I	1.098	1.00
80_E	241_L	1.098	1.00
9_S	12_F	1.096	1.00
8_W	18_W	1.096	1.00
308_M	312_I	1.095	1.00
185_K	197_L	1.095	1.00
383_N	386_K	1.094	1.00
207_A	236_N	1.094	1.00
360_S	375_T	1.093	1.00
349_I	388_L	1.088	1.00
343_E	346_G	1.080	1.00
321_N	382_I	1.073	1.00
64_E	326_L	1.069	1.00
60_S	186_K	1.063	1.00
42_Y	326_L	1.063	1.00
284_P	389_L	1.060	1.00
317_M	361_G	1.060	1.00
57_F	190_T	1.056	1.00
70_L	141_F	1.056	1.00
27_D	122_L	1.053	1.00
54_I	58_L	1.049	1.00
317_M	320_K	1.049	1.00
61_T	179_E	1.049	1.00
154_L	264_D	1.045	1.00
295_P	311_S	1.044	1.00
312_I	330_D	1.037	1.00
172_D	176_Q	1.036	1.00
245_I	255_P	1.032	1.00
53_I	186_K	1.029	1.00
291_V	318_F	1.025	1.00
217_T	221_D	1.024	1.00
351_L	365_I	1.021	1.00
293_V	314_I	1.016	1.00
313_E	316_E	1.013	1.00
363_V	378_K	1.011	1.00
45_K	59_D	1.010	1.00
292_V	326_L	1.010	1.00
109_R	112_S	1.009	1.00
56_N	60_S	1.008	1.00
56_N	322_N	1.004	1.00
351_L	363_V	1.002	1.00
54_I	187_I	0.991	0.99
48_Q	65_E	0.991	0.99
186_K	189_D	0.988	0.99
328_D	338_K	0.987	0.99
44_F	67_L	0.981	0.99
177_V	213_Y	0.978	0.99
381_A	385_I	0.977	0.99
284_P	385_I	0.976	0.99
343_E	366_K	0.972	0.99
367_R	388_L	0.972	0.99
175_K	178_E	0.971	0.99
63_H	176_Q	0.970	0.99
376_V	384_K	0.968	0.99
293_V	353_I	0.965	0.99
238_S	255_P	0.959	0.99
66_V	134_Y	0.954	0.99
357_E	375_T	0.953	0.99
133_K	179_E	0.951	0.99
170_Y	256_H	0.949	0.99
206_F	209_A	0.946	0.99
357_E	360_S	0.945	0.99
361_G	378_K	0.945	0.99
50_I	266_A	0.940	0.99
210_L	214_A	0.940	0.99
163_A	180_A	0.937	0.99
243_F	255_P	0.936	0.99
275_G	281_V	0.935	0.99
244_K	254_Y	0.935	0.99
378_K	382_I	0.934	0.99
293_V	327_T	0.934	0.99
292_V	345_K	0.929	0.99
328_D	331_T	0.927	0.99
139_S	156_E	0.925	0.99
326_L	347_V	0.922	0.99
20_L	151_M	0.917	0.99
319_N	325_C	0.909	0.99
121_N	242_D	0.908	0.99
112_S	136_Q	0.908	0.99
281_V	368_R	0.907	0.99
370_N	374_Y	0.907	0.99
118_T	121_N	0.904	0.99
313_E	378_K	0.894	0.99
32_P	38_V	0.893	0.99
61_T	186_K	0.889	0.99
117_T	254_Y	0.889	0.99
333_K	337_E	0.885	0.99
290_K	323_I	0.884	0.99
117_T	166_V	0.883	0.99
370_N	373_S	0.878	0.99
51_I	67_L	0.872	0.99
239_K	254_Y	0.871	0.99
215_F	219_M	0.870	0.99
70_L	139_S	0.869	0.99
291_V	385_I	0.868	0.99
181_I	197_L	0.863	0.99
330_D	333_K	0.860	0.98
297_P	355_P	0.860	0.98
47_R	67_L	0.859	0.98
323_I	389_L	0.850	0.98
337_E	341_I	0.847	0.98
352_E	364_F	0.846	0.98
363_V	381_A	0.845	0.98
202_E	205_R	0.844	0.98
63_H	163_A	0.842	0.98
19_V	23_G	0.841	0.98
195_Y	199_E	0.836	0.98
376_V	380_E	0.836	0.98
60_S	320_K	0.834	0.98
90_E	93_W	0.830	0.98
108_L	141_F	0.826	0.98
308_M	330_D	0.826	0.98
316_E	319_N	0.824	0.98
98_G	104_V	0.824	0.98
47_R	264_D	0.813	0.98
53_I	57_F	0.811	0.98
151_M	154_L	0.805	0.98
49_N	290_K	0.805	0.98
39_W	43_G	0.805	0.98
201_P	204_D	0.802	0.98
310_Y	357_E	0.800	0.98
295_P	307_V	0.799	0.98
235_Q	238_S	0.794	0.97
39_W	264_D	0.792	0.97
179_E	182_E	0.789	0.97
55_R	59_D	0.788	0.97
318_F	382_I	0.788	0.97
366_K	373_S	0.787	0.97
166_V	255_P	0.785	0.97
361_G	377_R	0.783	0.97
318_F	323_I	0.781	0.97
173_A	233_L	0.781	0.97
211_H	232_H	0.776	0.97
188_F	215_F	0.775	0.97
154_L	157_I	0.775	0.97
70_L	108_L	0.775	0.97
310_Y	378_K	0.774	0.97
64_E	132_K	0.773	0.97
314_I	317_M	0.772	0.97
291_V	325_C	0.770	0.97
59_D	319_N	0.769	0.97
97_G	143_Y	0.768	0.97
199_E	211_H	0.768	0.97
376_V	381_A	0.766	0.97
311_S	353_I	0.764	0.97
86_G	205_R	0.758	0.97
40_M	119_M	0.758	0.97
310_Y	361_G	0.757	0.97
181_I	230_V	0.757	0.97
27_D	123_W	0.755	0.97
274_H	281_V	0.752	0.97
165_T	180_A	0.751	0.96
68_F	116_I	0.750	0.96
344_I	368_R	0.748	0.96
120_E	134_Y	0.747	0.96
24_E	27_D	0.747	0.96
13_S	17_D	0.747	0.96
181_I	213_Y	0.745	0.96
73_P	104_V	0.745	0.96
295_P	308_M	0.741	0.96
9_S	13_S	0.740	0.96
75_D	104_V	0.735	0.96
218_I	222_G	0.733	0.96
50_I	267_V	0.732	0.96
374_Y	380_E	0.730	0.96
87_F	207_A	0.728	0.96
371_F	374_Y	0.723	0.96
321_N	383_N	0.721	0.96
170_Y	174_Q	0.720	0.96
80_E	242_D	0.719	0.95
294_I	342_W	0.719	0.95
387_E	390_N	0.718	0.95
191_L	286_V	0.718	0.95
13_S	275_G	0.715	0.95
206_F	214_A	0.713	0.95
165_T	176_Q	0.712	0.95
357_E	362_T	0.712	0.95
95_T	98_G	0.710	0.95
238_S	254_Y	0.709	0.95
298_A	312_I	0.708	0.95
208_G	212_T	0.707	0.95
139_S	159_T	0.706	0.95
61_T	133_K	0.706	0.95
353_I	363_V	0.705	0.95
46_L	282_L	0.704	0.95
179_E	183_I	0.703	0.95
234_G	238_S	0.702	0.95
61_T	182_E	0.701	0.95
238_S	241_L	0.699	0.95
271_I	274_H	0.698	0.95
363_V	376_V	0.698	0.95
61_T	180_A	0.697	0.95
238_S	243_F	0.696	0.95
157_I	262_I	0.696	0.95
75_D	80_E	0.690	0.94
314_I	318_F	0.687	0.94
235_Q	254_Y	0.686	0.94
20_L	26_Y	0.685	0.94
48_Q	55_R	0.683	0.94
32_P	118_T	0.680	0.94
52_G	55_R	0.680	0.94
15_W	275_G	0.679	0.94
191_L	266_A	0.678	0.94
123_W	129_Q	0.677	0.94
10_K	13_S	0.676	0.94
58_L	180_A	0.675	0.94
215_F	222_G	0.675	0.94
180_A	183_I	0.672	0.94
219_M	222_G	0.671	0.94
93_W	107_A	0.670	0.93
78_R	84_I	0.664	0.93
236_N	240_A	0.664	0.93
323_I	382_I	0.663	0.93
22_E	344_I	0.663	0.93
58_L	163_A	0.662	0.93
59_D	65_E	0.661	0.93
25_F	271_I	0.661	0.93
195_Y	198_S	0.660	0.93
379_E	383_N	0.659	0.93
262_I	266_A	0.658	0.93
92_F	143_Y	0.656	0.93
284_P	381_A	0.655	0.93
125_K	129_Q	0.655	0.93
54_I	264_D	0.655	0.93
38_V	119_M	0.651	0.92
71_L	92_F	0.650	0.92
216_D	219_M	0.649	0.92
295_P	338_K	0.648	0.92
353_I	357_E	0.646	0.92
289_I	292_V	0.644	0.92
84_I	105_K	0.644	0.92
19_V	22_E	0.644	0.92
335_P	343_E	0.641	0.92
58_L	135_Y	0.641	0.92
31_Y	36_M	0.638	0.92
247_K	252_L	0.638	0.92
50_I	286_V	0.635	0.92
169_T	175_K	0.632	0.91
62_G	319_N	0.631	0.91
36_M	71_L	0.631	0.91
364_F	373_S	0.631	0.91
195_Y	200_R	0.631	0.91
214_A	217_T	0.630	0.91
169_T	248_K	0.628	0.91
24_E	345_K	0.627	0.91
54_I	137_I	0.626	0.91
333_K	341_I	0.626	0.91
364_F	372_K	0.625	0.91
356_R	375_T	0.625	0.91
125_K	132_K	0.624	0.91
160_F	258_T	0.624	0.91
33_I	106_L	0.624	0.91
352_E	373_S	0.621	0.91
28_Y	37_G	0.621	0.91
184_Y	260_Y	0.621	0.91
337_E	340_Y	0.621	0.91
212_T	236_N	0.620	0.91
367_R	374_Y	0.619	0.90
215_F	227_I	0.618	0.90
295_P	298_A	0.617	0.90
152_I	268_A	0.616	0.90
193_I	269_S	0.616	0.90
355_P	358_L	0.614	0.90
25_F	46_L	0.612	0.90
298_A	332_E	0.612	0.90
212_T	229_T	0.611	0.90
287_A	348_P	0.610	0.90
367_R	371_F	0.607	0.90
218_I	233_L	0.607	0.90
200_R	229_T	0.607	0.90
236_N	239_K	0.607	0.90
178_E	181_I	0.605	0.89
276_D	368_R	0.604	0.89
121_N	241_L	0.603	0.89
68_F	115_A	0.603	0.89
188_F	266_A	0.602	0.89
117_T	257_Q	0.597	0.89
92_F	108_L	0.596	0.89
202_E	210_L	0.596	0.89
198_S	216_D	0.595	0.89
71_L	106_L	0.595	0.89
25_F	282_L	0.595	0.89
295_P	335_P	0.593	0.89
118_T	122_L	0.592	0.88
51_I	55_R	0.592	0.88
87_F	206_F	0.592	0.88
171_D	174_Q	0.591	0.88
53_I	285_S	0.590	0.88
96_K	99_S	0.588	0.88
310_Y	314_I	0.588	0.88
76_L	118_T	0.588	0.88
15_W	18_W	0.587	0.88
77_L	115_A	0.586	0.88
54_I	262_I	0.585	0.88
149_R	152_I	0.585	0.88
270_V	274_H	0.585	0.88
6_E	13_S	0.582	0.88
189_D	194_P	0.582	0.88
74_E	107_A	0.579	0.87
39_W	47_R	0.577	0.87
159_T	264_D	0.577	0.87
140_V	160_F	0.577	0.87
96_K	105_K	0.575	0.87
294_I	338_K	0.573	0.87
20_L	271_I	0.573	0.87
123_W	134_Y	0.572	0.87
120_E	255_P	0.570	0.87
299_K	307_V	0.569	0.86
225_M	269_S	0.569	0.86
116_I	134_Y	0.569	0.86
196_V	199_E	0.568	0.86
294_I	352_E	0.568	0.86
386_K	389_L	0.566	0.86
210_L	256_H	0.566	0.86
23_G	345_K	0.565	0.86
205_R	208_G	0.563	0.86
196_V	200_R	0.561	0.86
219_M	225_M	0.560	0.86
296_I	336_G	0.559	0.85
350_R	373_S	0.557	0.85
24_E	42_Y	0.557	0.85
184_Y	188_F	0.557	0.85
122_L	125_K	0.556	0.85
25_F	151_M	0.556	0.85
81_S	87_F	0.555	0.85
202_E	212_T	0.554	0.85
177_V	230_V	0.553	0.85
202_E	209_A	0.552	0.85
44_F	51_I	0.552	0.85
205_R	212_T	0.552	0.85
331_T	334_T	0.552	0.85
199_E	213_Y	0.551	0.85
226_Q	229_T	0.551	0.85
58_L	137_I	0.550	0.85
334_T	338_K	0.548	0.84
95_T	99_S	0.548	0.84
274_H	369_D	0.547	0.84
367_R	372_K	0.547	0.84
309_K	316_E	0.546	0.84
318_F	381_A	0.546	0.84
58_L	260_Y	0.545	0.84
51_I	137_I	0.545	0.84
44_F	47_R	0.545	0.84
187_I	258_T	0.544	0.84
32_P	115_A	0.544	0.84
52_G	59_D	0.542	0.84
362_T	380_E	0.542	0.84
133_K	163_A	0.541	0.84
140_V	260_Y	0.538	0.83
284_P	367_R	0.538	0.83
184_Y	259_S	0.538	0.83
94_V	97_G	0.536	0.83
27_D	119_M	0.534	0.83
374_Y	378_K	0.532	0.83
58_L	183_I	0.531	0.82
246_Q	251_S	0.530	0.82
164_H	226_Q	0.527	0.82
170_Y	253_D	0.526	0.82
112_S	138_V	0.525	0.82
50_I	260_Y	0.524	0.82
16_F	271_I	0.524	0.82
170_Y	210_L	0.523	0.82
239_K	242_D	0.522	0.81
32_P	73_P	0.520	0.81
58_L	161_K	0.519	0.81
174_Q	211_H	0.519	0.81
374_Y	388_L	0.518	0.81
205_R	209_A	0.518	0.81
77_L	91_V	0.518	0.81
53_I	191_L	0.517	0.81
308_M	327_T	0.515	0.81
281_V	343_E	0.512	0.80
53_I	286_V	0.512	0.80
282_L	287_A	0.512	0.80
322_N	386_K	0.512	0.80
14_E	18_W	0.511	0.80
226_Q	258_T	0.509	0.80
96_K	143_Y	0.509	0.80
343_E	369_D	0.508	0.80
33_I	72_I	0.507	0.80
133_K	180_A	0.507	0.80
381_A	386_K	0.506	0.79
124_L	252_L	0.505	0.79
46_L	49_N	0.505	0.79
23_G	344_I	0.503	0.79
373_S	376_V	0.502	0.79
296_I	307_V	0.501	0.79
311_S	315_C	0.500	0.79

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4ncxA	2	0.9085	100	0.195	Contact Map
3ialA	2	0.9917	100	0.196	Contact Map
1hc7A	2	0.9563	100	0.206	Contact Map
1nj1A	2	0.9626	100	0.215	Contact Map
4hvcA	2	0.9792	100	0.219	Contact Map
1nj8A	2	0.9418	100	0.231	Contact Map
1qf6A	1	0.7838	100	0.459	Contact Map
1nyrA	2	0.7838	100	0.466	Contact Map
2i4lA	2	0.7692	100	0.472	Contact Map
2j3lA	2	0.7838	100	0.494	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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