Q981B3 - SULSO - GREMLIN Contacts

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Q981B3 - SOJ protein (Soj)

UniProt:	Q981B3	Sequences: MIVTVINQKGGVGKTTTSVNLSYYLSKEKKTGLLDLDPEGGATISYGMKRELKELPLGEKSVNIFNVEVFPAHIGLLKLELNGDVEEISNKIKEIGKQFDFLVIDTPPNLGTLAISAMLVADRIVSPVTPQPLALEAIKNLDSRLKSIGKNAYSFTNFSKKVVKLDNLSSVKFTEITIPPSRLFIEASRLGVPALRYEEVRIKKPKLANYYQQLAKVISE	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	220 (210)
Sequences:	16606 (10994)
Seq/√Len:	758.7

ECOLI - BCSQ MIND MRP
BACSU - SOJ YLXH SALA MIND YVEL YWQD
HALSA - Q9HHZ7 Q9HHU2 Q9HQY5 O51969 Q9HQJ7 O51991 Q9HHL5 Q9HNF1 O54573
SULSO - Q981B3 Q97ZW4 Q97VX7 Q97WA2 Q97YM8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
68_E	99_F	4.348	1.00
179_P	206_K	4.345	1.00
18_S	103_V	3.990	1.00
25_L	101_F	3.457	1.00
27_K	212_Q	3.362	1.00
29_K	101_F	3.211	1.00
17_T	207_L	3.065	1.00
193_P	196_R	3.040	1.00
32_G	99_F	2.934	1.00
4_T	121_A	2.830	1.00
19_V	42_A	2.823	1.00
33_L	42_A	2.773	1.00
45_S	133_L	2.260	1.00
183_L	186_E	2.231	1.00
30_K	100_D	2.221	1.00
22_S	33_L	2.199	1.00
18_S	33_L	2.150	1.00
24_Y	211_Y	2.145	1.00
24_Y	195_L	2.142	1.00
5_V	21_L	2.119	1.00
114_A	117_A	2.050	1.00
25_L	103_V	2.032	1.00
27_K	208_A	2.020	1.00
176_I	213_Q	1.952	1.00
44_I	136_E	1.884	1.00
113_L	117_A	1.791	1.00
26_S	67_V	1.788	1.00
129_T	165_L	1.787	1.00
19_V	45_S	1.781	1.00
36_L	117_A	1.742	1.00
22_S	103_V	1.698	1.00
5_V	125_V	1.682	1.00
123_R	159_S	1.658	1.00
176_I	210_Y	1.635	1.00
4_T	106_T	1.619	1.00
175_E	213_Q	1.591	1.00
41_G	137_A	1.572	1.00
29_K	100_D	1.524	1.00
182_R	186_E	1.517	1.00
21_L	214_L	1.516	1.00
26_S	66_N	1.513	1.00
181_S	203_K	1.496	1.00
131_Q	134_A	1.496	1.00
36_L	104_I	1.491	1.00
19_V	46_Y	1.484	1.00
166_D	170_S	1.459	1.00
201_R	204_K	1.448	1.00
41_G	133_L	1.445	1.00
6_I	141_L	1.432	1.00
159_S	162_V	1.427	1.00
5_V	18_S	1.378	1.00
24_Y	27_K	1.377	1.00
25_L	31_T	1.375	1.00
46_Y	67_V	1.375	1.00
21_L	211_Y	1.374	1.00
22_S	42_A	1.365	1.00
25_L	211_Y	1.365	1.00
124_I	145_L	1.322	1.00
32_G	102_L	1.312	1.00
205_P	208_A	1.292	1.00
186_E	190_L	1.291	1.00
15_T	45_S	1.266	1.00
184_F	203_K	1.261	1.00
166_D	169_S	1.244	1.00
6_I	106_T	1.244	1.00
29_K	215_A	1.204	1.00
30_K	98_Q	1.203	1.00
192_V	197_Y	1.193	1.00
165_L	180_P	1.188	1.00
209_N	212_Q	1.180	1.00
208_A	212_Q	1.177	1.00
59_E	63_N	1.176	1.00
22_S	67_V	1.174	1.00
174_T	177_T	1.173	1.00
183_L	198_E	1.167	1.00
133_L	188_S	1.166	1.00
20_N	23_Y	1.164	1.00
20_N	195_L	1.164	1.00
74_I	140_N	1.156	1.00
210_Y	214_L	1.150	1.00
86_E	89_S	1.145	1.00
48_M	69_V	1.142	1.00
131_Q	185_I	1.128	1.00
17_T	162_V	1.127	1.00
34_L	104_I	1.110	1.00
68_E	97_K	1.109	1.00
17_T	21_L	1.106	1.00
80_E	111_G	1.102	1.00
199_E	204_K	1.097	1.00
5_V	17_T	1.087	1.00
83_G	86_E	1.080	1.00
34_L	102_L	1.076	1.00
38_P	113_L	1.075	1.00
15_T	42_A	1.072	1.00
64_I	196_R	1.071	1.00
91_K	94_E	1.067	1.00
192_V	196_R	1.057	1.00
46_Y	69_V	1.046	1.00
114_A	119_L	1.036	1.00
89_S	93_K	1.034	1.00
39_E	108_P	1.027	1.00
162_V	165_L	1.022	1.00
178_I	203_K	1.019	1.00
69_V	74_I	1.019	1.00
9_K	133_L	1.018	1.00
153_Y	156_T	1.015	1.00
143_S	146_K	0.994	1.00
206_K	209_N	0.993	1.00
101_F	215_A	0.985	1.00
129_T	134_A	0.983	1.00
209_N	213_Q	0.980	1.00
116_S	119_L	0.977	1.00
6_I	114_A	0.975	1.00
181_S	201_R	0.967	1.00
27_K	215_A	0.963	1.00
130_P	163_V	0.961	1.00
16_T	184_F	0.958	1.00
12_V	134_A	0.956	1.00
24_Y	208_A	0.951	1.00
204_K	208_A	0.951	1.00
60_K	63_N	0.933	1.00
203_K	206_K	0.933	1.00
195_L	208_A	0.930	1.00
17_T	127_P	0.928	1.00
202_I	205_P	0.923	1.00
20_N	193_P	0.913	1.00
3_V	101_F	0.911	1.00
190_L	197_Y	0.907	1.00
16_T	188_S	0.901	1.00
198_E	201_R	0.901	1.00
23_Y	195_L	0.901	1.00
40_G	72_A	0.889	1.00
114_A	118_M	0.888	1.00
106_T	117_A	0.884	1.00
99_F	102_L	0.883	1.00
87_E	91_K	0.882	1.00
3_V	123_R	0.876	1.00
14_K	108_P	0.872	1.00
91_K	95_I	0.869	1.00
31_T	101_F	0.867	1.00
113_L	119_L	0.867	1.00
155_F	158_F	0.865	1.00
126_S	138_I	0.860	1.00
3_V	214_L	0.848	1.00
126_S	141_L	0.848	1.00
40_G	43_T	0.842	1.00
194_A	204_K	0.831	1.00
43_T	73_H	0.813	1.00
90_N	94_E	0.806	1.00
9_K	14_K	0.796	1.00
68_E	71_P	0.794	1.00
96_G	122_D	0.789	1.00
18_S	42_A	0.789	1.00
202_I	206_K	0.786	1.00
173_F	213_Q	0.784	1.00
13_G	17_T	0.781	1.00
13_G	164_K	0.779	1.00
41_G	134_A	0.778	1.00
23_Y	196_R	0.762	1.00
126_S	142_D	0.758	1.00
84_D	87_E	0.757	1.00
44_I	132_P	0.749	1.00
178_I	206_K	0.745	1.00
31_T	67_V	0.744	1.00
170_S	173_F	0.743	1.00
157_N	160_K	0.743	1.00
86_E	90_N	0.739	1.00
41_G	136_E	0.734	1.00
159_S	163_V	0.734	1.00
8_Q	144_R	0.732	1.00
132_P	136_E	0.731	1.00
5_V	103_V	0.730	1.00
160_K	165_L	0.730	1.00
43_T	71_P	0.720	1.00
115_I	144_R	0.717	1.00
4_T	117_A	0.708	1.00
64_I	67_V	0.705	1.00
54_E	59_E	0.705	1.00
88_I	91_K	0.705	1.00
106_T	114_A	0.694	1.00
194_A	203_K	0.694	1.00
33_L	46_Y	0.693	1.00
184_F	188_S	0.691	1.00
161_K	165_L	0.691	1.00
167_N	170_S	0.690	1.00
162_V	210_Y	0.687	1.00
83_G	90_N	0.685	1.00
92_I	120_V	0.684	1.00
119_L	152_A	0.683	1.00
103_V	214_L	0.682	1.00
96_G	102_L	0.680	1.00
23_Y	193_P	0.673	1.00
139_K	169_S	0.673	1.00
23_Y	67_V	0.668	1.00
7_N	12_V	0.667	1.00
50_R	53_K	0.666	1.00
195_L	204_K	0.665	1.00
31_T	100_D	0.662	1.00
30_K	99_F	0.658	1.00
179_P	202_I	0.656	1.00
111_G	114_A	0.655	1.00
205_P	209_N	0.650	1.00
142_D	146_K	0.647	1.00
113_L	116_S	0.647	1.00
63_N	98_Q	0.647	1.00
9_K	12_V	0.646	1.00
157_N	162_V	0.646	1.00
181_S	202_I	0.645	1.00
27_K	211_Y	0.645	1.00
187_A	194_A	0.643	1.00
151_N	155_F	0.642	1.00
159_S	164_K	0.642	1.00
174_T	213_Q	0.641	1.00
117_A	121_A	0.636	1.00
19_V	33_L	0.632	1.00
158_F	161_K	0.632	1.00
58_G	62_V	0.632	1.00
76_L	79_L	0.631	1.00
15_T	41_G	0.628	1.00
200_V	205_P	0.628	1.00
139_K	143_S	0.623	1.00
59_E	62_V	0.623	1.00
207_L	210_Y	0.618	1.00
160_K	164_K	0.617	1.00
58_G	61_S	0.617	1.00
211_Y	215_A	0.617	1.00
26_S	64_I	0.617	1.00
85_V	116_S	0.617	1.00
8_Q	109_N	0.617	1.00
160_K	174_T	0.616	1.00
186_E	197_Y	0.615	1.00
42_A	69_V	0.615	1.00
182_R	185_I	0.614	1.00
31_T	103_V	0.613	1.00
28_E	66_N	0.610	1.00
185_I	188_S	0.610	1.00
40_G	73_H	0.609	1.00
143_S	147_S	0.608	1.00
161_K	164_K	0.601	1.00
68_E	98_Q	0.601	1.00
17_T	178_I	0.600	1.00
110_L	114_A	0.600	1.00
7_N	137_A	0.592	1.00
135_L	139_K	0.590	1.00
134_A	137_A	0.588	1.00
49_K	53_K	0.588	1.00
154_S	158_F	0.588	1.00
153_Y	158_F	0.587	1.00
24_Y	207_L	0.587	1.00
108_P	137_A	0.587	1.00
70_F	74_I	0.586	1.00
14_K	37_D	0.583	1.00
63_N	94_E	0.583	1.00
34_L	92_I	0.583	1.00
90_N	150_K	0.582	1.00
65_F	68_E	0.581	1.00
159_S	174_T	0.581	1.00
160_K	163_V	0.580	1.00
24_Y	204_K	0.579	1.00
8_Q	108_P	0.578	1.00
132_P	189_R	0.576	1.00
32_G	95_I	0.576	1.00
118_M	124_I	0.575	1.00
84_D	113_L	0.574	1.00
21_L	210_Y	0.574	1.00
72_A	76_L	0.574	1.00
164_K	207_L	0.571	1.00
185_I	189_R	0.569	1.00
16_T	20_N	0.569	1.00
23_Y	65_F	0.566	1.00
148_I	153_Y	0.566	1.00
93_K	96_G	0.563	1.00
6_I	124_I	0.562	1.00
88_I	92_I	0.559	1.00
20_N	207_L	0.559	1.00
38_P	137_A	0.557	1.00
181_S	206_K	0.556	1.00
140_N	143_S	0.556	1.00
122_D	155_F	0.555	1.00
70_F	95_I	0.555	1.00
147_S	150_K	0.552	1.00
149_G	153_Y	0.552	1.00
17_T	164_K	0.551	1.00
156_T	159_S	0.551	1.00
78_K	81_L	0.548	1.00
160_K	180_P	0.548	1.00
129_T	185_I	0.547	1.00
36_L	113_L	0.545	1.00
34_L	88_I	0.544	1.00
32_G	68_E	0.543	1.00
141_L	145_L	0.539	1.00
199_E	205_P	0.536	1.00
4_T	104_I	0.536	1.00
83_G	87_E	0.536	1.00
90_N	93_K	0.533	1.00
110_L	144_R	0.530	1.00
17_T	125_V	0.528	1.00
77_L	80_E	0.526	1.00
187_A	197_Y	0.526	1.00
156_T	160_K	0.524	1.00
7_N	126_S	0.521	1.00
26_S	31_T	0.520	1.00
7_N	14_K	0.518	1.00
86_E	119_L	0.518	1.00
125_V	214_L	0.515	1.00
89_S	92_I	0.514	1.00
178_I	210_Y	0.513	1.00
149_G	157_N	0.510	1.00
72_A	75_G	0.509	1.00
54_E	60_K	0.506	1.00
8_Q	141_L	0.506	1.00
20_N	194_A	0.506	1.00
23_Y	26_S	0.505	1.00
8_Q	41_G	0.503	1.00
128_V	138_I	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4pfsA	1	0.9227	100	0.257	Contact Map
3pg5A	2	0.8864	100	0.272	Contact Map
3ez2A	2	1	100	0.279	Contact Map
3kjhA	2	0.9773	100	0.281	Contact Map
3ez9A	2	0.8273	100	0.281	Contact Map
1wcv1	2	0.9818	100	0.289	Contact Map
4rz3A	2	0.9818	100	0.297	Contact Map
3ea0A	2	0.9727	100	0.303	Contact Map
1hyqA	1	0.9682	100	0.305	Contact Map
1g3qA	1	0.9818	100	0.306	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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