GREMLIN Database
Q980J4 - Thiamine biosynthesis protein related protein (ThiF)
UniProt: Q980J4 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 333 (258)
Sequences: 1718 (1078)
Seq/√Len: 67.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
111_E131_S3.8301.00
109_F121_V3.6871.00
68_I79_A3.5561.00
158_F241_M3.5361.00
157_V174_I3.4651.00
89_E134_K3.3841.00
116_K124_K3.2901.00
203_C206_C3.2051.00
123_A127_S3.1631.00
71_C92_I3.1571.00
65_K89_E3.0831.00
42_L62_N2.9901.00
240_L243_N2.9481.00
78_V158_F2.9451.00
203_C274_C2.9341.00
274_C277_C2.8601.00
95_A141_I2.8141.00
76_T108_L2.7521.00
156_Y241_M2.7261.00
113_D116_K2.7201.00
89_E136_N2.6801.00
64_L245_L2.6351.00
91_T154_K2.4191.00
206_C274_C2.3711.00
123_A137_Y2.3611.00
112_N128_L2.3031.00
203_C277_C2.2811.00
53_L57_I2.2761.00
83_A133_I2.1601.00
126_I135_I2.1471.00
184_G233_T2.1411.00
108_L122_C2.0941.00
117_P139_I2.0211.00
252_S265_R1.9201.00
172_D205_S1.9101.00
91_T138_I1.8761.00
138_I154_K1.8701.00
79_A92_I1.8491.00
61_L242_L1.8451.00
53_L246_R1.8361.00
193_A236_L1.8151.00
79_A126_I1.7741.00
156_Y245_L1.7361.00
153_D179_K1.7191.00
49_Q104_H1.7051.00
142_L147_V1.6800.99
110_D125_K1.5920.99
42_L132_S1.5810.99
108_L126_I1.5800.99
195_L244_H1.5730.99
46_Y130_N1.5600.99
65_K91_T1.5530.99
236_L263_I1.5420.99
231_E235_T1.5360.99
59_Q63_E1.5040.99
151_I174_I1.4800.99
163_S166_Y1.4640.98
206_C277_C1.4620.98
231_E234_A1.4280.98
69_A170_L1.4170.98
175_V276_T1.4140.98
161_L170_L1.4060.98
100_I114_V1.3930.98
161_L166_Y1.3750.98
110_D128_L1.3590.98
127_S133_I1.3560.97
47_S51_I1.3450.97
80_E107_H1.3390.97
156_Y182_I1.3120.97
120_E139_I1.3050.97
93_V142_L1.3010.97
151_I157_V1.2900.97
281_E286_Y1.2880.97
157_V179_K1.2760.96
168_L209_D1.2720.96
182_I241_M1.2610.96
148_E177_L1.2560.96
113_D125_K1.2410.96
148_E173_A1.2270.95
140_D149_R1.2050.95
83_A126_I1.2000.95
173_A177_L1.1960.95
119_A137_Y1.1780.94
113_D124_K1.1680.94
262_K265_R1.1660.94
67_L93_V1.1640.94
239_N256_I1.1620.94
119_A139_I1.1480.93
83_A90_L1.1470.93
102_N118_K1.1360.93
81_L231_E1.1350.93
112_N124_K1.1250.93
239_N258_S1.1190.93
232_L256_I1.1180.93
62_N132_S1.1110.92
174_I181_L1.1070.92
232_L235_T1.0920.92
227_P231_E1.0910.92
127_S134_K1.0890.92
191_G233_T1.0860.91
67_L154_K1.0820.91
118_K162_D1.0700.91
96_D105_R1.0640.91
39_H42_L1.0610.90
120_E124_K1.0480.90
289_I294_C1.0460.90
67_L151_I1.0440.90
96_D118_K1.0400.90
148_E176_K1.0340.89
76_T80_E1.0330.89
120_E137_Y1.0310.89
50_L55_L1.0280.89
96_D162_D1.0260.89
288_K292_P1.0140.88
52_V189_E1.0140.88
69_A147_V1.0120.88
79_A122_C1.0040.88
123_A135_I1.0020.88
289_I293_K0.9860.87
102_N162_D0.9860.87
68_I82_L0.9840.87
230_V234_A0.9830.87
151_I177_L0.9820.87
289_I292_P0.9780.86
228_L232_L0.9740.86
234_A238_V0.9740.86
232_L239_N0.9730.86
71_C90_L0.9710.86
281_E284_F0.9650.86
71_C122_C0.9650.86
282_Y286_Y0.9630.86
49_Q58_Q0.9610.86
278_V281_E0.9570.85
72_G162_D0.9470.85
158_F184_G0.9370.84
125_K128_L0.9340.84
110_D113_D0.9320.84
272_S279_L0.9250.83
249_P259_R0.9130.82
46_Y133_I0.9070.82
149_R152_S0.8990.81
231_E238_V0.8970.81
287_Q290_S0.8950.81
254_F263_I0.8940.81
168_L205_S0.8940.81
182_I244_H0.8930.81
77_A80_E0.8890.81
155_D179_K0.8870.81
288_K291_K0.8840.80
69_A93_V0.8820.80
169_L205_S0.8760.80
105_R162_D0.8710.79
184_G237_Q0.8690.79
142_L177_L0.8690.79
286_Y289_I0.8660.79
124_K128_L0.8630.79
108_L121_V0.8630.79
186_I229_I0.8620.79
130_N133_I0.8620.79
78_V241_M0.8600.78
90_L126_I0.8520.78
88_K130_N0.8500.78
109_F113_D0.8480.78
71_C119_A0.8450.77
68_I92_I0.8440.77
152_S177_L0.8400.77
78_V160_A0.8380.77
228_L235_T0.8340.76
96_D102_N0.8310.76
62_N131_S0.8300.76
65_K136_N0.8290.76
43_V55_L0.8220.75
105_R118_K0.8180.75
120_E123_A0.8100.74
227_P232_L0.8080.74
79_A108_L0.8060.74
108_L125_K0.8040.74
229_I232_L0.8010.73
72_G105_R0.8010.73
282_Y285_L0.7990.73
184_G191_G0.7970.73
83_A135_I0.7960.73
218_N223_I0.7960.73
212_D217_G0.7820.72
227_P234_A0.7810.71
163_S167_K0.7720.71
127_S135_I0.7710.71
219_S222_V0.7640.70
232_L236_L0.7630.70
43_V50_L0.7630.70
113_D198_P0.7600.69
195_L243_N0.7570.69
279_L283_P0.7560.69
147_V173_A0.7540.69
197_D201_T0.7510.69
97_V117_P0.7490.68
102_N105_R0.7480.68
71_C79_A0.7460.68
117_P120_E0.7440.68
229_I235_T0.7330.67
197_D200_Q0.7310.66
80_E84_R0.7290.66
61_L88_K0.7240.66
276_T281_E0.7230.66
225_T230_V0.7230.66
93_V147_V0.7210.65
104_H185_G0.7210.65
84_R129_I0.7160.65
80_E110_D0.7140.65
160_A164_L0.7130.64
148_E152_S0.7080.64
195_L240_L0.7080.64
74_L184_G0.7050.64
230_V235_T0.7040.63
72_G96_D0.7010.63
38_L42_L0.6960.63
93_V151_I0.6940.62
133_I247_G0.6930.62
270_K278_V0.6910.62
46_Y58_Q0.6850.61
194_K225_T0.6850.61
170_L181_L0.6850.61
101_T118_K0.6840.61
113_D121_V0.6830.61
227_P230_V0.6820.61
159_D224_G0.6820.61
168_L172_D0.6820.61
158_F182_I0.6810.61
280_N283_P0.6790.61
58_Q61_L0.6750.60
290_S293_K0.6720.60
163_S204_L0.6720.60
239_N242_L0.6700.60
68_I247_G0.6700.60
226_T258_S0.6680.59
147_V230_V0.6680.59
227_P235_T0.6650.59
151_I173_A0.6630.59
124_K127_S0.6630.59
171_N206_C0.6600.59
248_N259_R0.6590.58
242_L246_R0.6580.58
69_A101_T0.6560.58
190_Y194_K0.6550.58
77_A81_L0.6540.58
283_P286_Y0.6440.57
91_T136_N0.6420.56
72_G75_G0.6400.56
171_N203_C0.6390.56
284_F287_Q0.6380.56
47_S50_L0.6350.56
164_L204_L0.6340.56
96_D220_C0.6320.55
61_L246_R0.6320.55
142_L151_I0.6290.55
245_L280_N0.6280.55
273_Q279_L0.6280.55
81_L107_H0.6250.54
229_I234_A0.6250.54
157_V170_L0.6230.54
278_V283_P0.6220.54
202_S206_C0.6210.54
98_V121_V0.6200.54
183_Y193_A0.6190.54
60_R63_E0.6190.54
101_T105_R0.6180.54
243_N249_P0.6170.53
287_Q291_K0.6160.53
72_G102_N0.6150.53
106_V122_C0.6120.53
60_R267_N0.6110.53
77_A107_H0.6110.53
81_L204_L0.6090.53
220_C224_G0.6090.53
123_A280_N0.6070.52
184_G193_A0.6050.52
169_L172_D0.6050.52
93_V169_L0.6050.52
72_G118_K0.6020.52
144_E287_Q0.6020.52
283_P287_Q0.5990.51
55_L223_I0.5980.51
40_Y47_S0.5980.51
165_Y216_I0.5970.51
60_R64_L0.5970.51
243_N246_R0.5970.51
159_D167_K0.5950.51
159_D163_S0.5910.50
67_L91_T0.5910.50
274_C278_V0.5900.50
211_S242_L0.5890.50
77_A234_A0.5870.50
121_V126_I0.5840.50
192_S204_L0.5840.50
94_D119_A0.5840.50
160_A223_I0.5830.49
290_S294_C0.5820.49
53_L189_E0.5810.49
49_Q84_R0.5800.49
65_K125_K0.5790.49
189_E228_L0.5770.49
57_I246_R0.5760.49
44_E47_S0.5750.49
276_T283_P0.5740.48
256_I261_L0.5710.48
159_D183_Y0.5700.48
116_K121_V0.5700.48
39_H255_Y0.5690.48
193_A233_T0.5680.48
198_P201_T0.5670.48
109_F264_E0.5660.47
281_E285_L0.5650.47
96_D101_T0.5650.47
276_T285_L0.5640.47
101_T162_D0.5640.47
67_L74_L0.5640.47
197_D244_H0.5620.47
104_H237_Q0.5620.47
78_V193_A0.5600.47
84_R104_H0.5590.47
49_Q229_I0.5560.46
244_H251_Y0.5550.46
76_T121_V0.5550.46
81_L238_V0.5540.46
40_Y139_I0.5530.46
103_L106_V0.5510.46
128_L165_Y0.5500.46
132_S152_S0.5490.45
77_A84_R0.5490.45
182_I195_L0.5490.45
194_K202_S0.5480.45
94_D118_K0.5470.45
64_L247_G0.5460.45
99_D102_N0.5450.45
236_L239_N0.5440.45
228_L234_A0.5430.45
73_A115_G0.5420.45
213_Q223_I0.5420.45
171_N225_T0.5400.44
271_N279_L0.5400.44
39_H132_S0.5400.44
77_A231_E0.5400.44
156_Y244_H0.5390.44
285_L289_I0.5340.44
69_A142_L0.5340.44
248_N251_Y0.5340.44
55_L59_Q0.5320.43
171_N276_T0.5320.43
287_Q292_P0.5320.43
147_V150_L0.5310.43
114_V214_D0.5300.43
164_L212_D0.5300.43
38_L43_V0.5290.43
165_Y188_G0.5270.43
159_D170_L0.5260.43
291_K294_C0.5260.43
235_T239_N0.5260.43
44_E188_G0.5260.43
63_E134_K0.5250.43
288_K293_K0.5250.43
94_D109_F0.5220.42
43_V47_S0.5210.42
65_K154_K0.5200.42
58_Q85_L0.5180.42
194_K268_I0.5160.42
221_D226_T0.5150.41
162_D167_K0.5150.41
171_N181_L0.5140.41
89_E153_D0.5140.41
239_N243_N0.5130.41
52_V254_F0.5120.41
125_K183_Y0.5110.41
73_A104_H0.5110.41
131_S135_I0.5100.41
143_D146_N0.5100.41
91_T179_K0.5070.41
49_Q85_L0.5060.40
145_E165_Y0.5060.40
124_K152_S0.5060.40
171_N277_C0.5060.40
282_Y289_I0.5040.40
250_D260_E0.5040.40
124_K275_K0.5020.40
284_F293_K0.5010.40
228_L239_N0.5010.40
236_L261_L0.5000.40
286_Y293_K0.5000.40
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1tt5B 1 0.9099 100 0.343 Contact Map
1zud1 2 0.7057 100 0.345 Contact Map
2nvuB 1 0.997 100 0.353 Contact Map
1jw9B 2 0.7027 100 0.357 Contact Map
4gslA 2 0.9099 100 0.367 Contact Map
3ruiA 2 0.8258 100 0.375 Contact Map
3h8vA 2 0.6366 100 0.378 Contact Map
3h5nA 3 0.8138 100 0.398 Contact Map
4nnjA 2 0.973 100 0.404 Contact Map
4ii2A 1 0.988 100 0.405 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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