Q97ZW7 - SULSO - GREMLIN Contacts

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Q97ZW7 - Uncharacterized protein

UniProt:	Q97ZW7	Sequences: MLLTYLGLGIILGLSMAAPPGPVNAMIANESMKSWLHGSSIGAGAMTADLIFFIIVYFIQGYIPTPIRNALYIIGGIFMLYLSYLTIRAKMPSKSIKGNYFIGLSMGLTNPYQISWWITVGISIIRSLSILIIPGFFVGILIWIIAFPKAVNMLGSKYVKYVKVISSVILVIFGVYLLYEGILNVI	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	186 (175)
Sequences:	12125 (8541)
Seq/√Len:	645.6

ECOLI - ARGO RHTC YAHN LEUE RHTB EAMB
BACSU - YISU YCGF YRHP
SULSO - Q97ZW7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
47_T	100_Y	5.160	1.00
83_S	171_V	3.601	1.00
84_Y	88_R	3.394	1.00
6_L	135_G	3.029	1.00
161_Y	164_V	2.991	1.00
69_N	72_Y	2.863	1.00
80_L	174_G	2.582	1.00
160_K	164_V	2.363	1.00
51_I	104_L	2.313	1.00
87_I	164_V	2.223	1.00
73_I	77_I	2.199	1.00
81_Y	85_L	2.177	1.00
76_G	178_L	2.060	1.00
87_I	167_S	2.003	1.00
26_M	41_I	1.983	1.00
30_E	33_K	1.895	1.00
168_V	172_I	1.877	1.00
85_L	88_R	1.868	1.00
31_S	38_G	1.866	1.00
155_G	159_V	1.844	1.00
10_I	139_G	1.794	1.00
90_K	164_V	1.792	1.00
48_A	108_L	1.770	1.00
12_L	120_V	1.761	1.00
80_L	171_V	1.750	1.00
83_S	167_S	1.743	1.00
37_H	99_N	1.729	1.00
23_V	45_A	1.717	1.00
76_G	174_G	1.688	1.00
24_N	147_F	1.674	1.00
38_G	148_P	1.623	1.00
68_R	72_Y	1.622	1.00
101_F	105_S	1.574	1.00
17_A	139_G	1.564	1.00
69_N	73_I	1.557	1.00
91_M	164_V	1.495	1.00
18_A	143_W	1.471	1.00
30_E	41_I	1.469	1.00
10_I	135_G	1.469	1.00
61_G	65_T	1.467	1.00
80_L	84_Y	1.466	1.00
50_L	137_F	1.466	1.00
43_A	100_Y	1.438	1.00
28_A	151_V	1.411	1.00
42_G	148_P	1.373	1.00
38_G	42_G	1.369	1.00
41_I	103_G	1.366	1.00
53_F	133_I	1.345	1.00
100_Y	104_L	1.340	1.00
31_S	148_P	1.338	1.00
161_Y	165_I	1.298	1.00
70_A	74_I	1.281	1.00
73_I	76_G	1.274	1.00
143_W	147_F	1.261	1.00
140_I	144_I	1.260	1.00
78_F	82_L	1.254	1.00
33_K	37_H	1.247	1.00
54_I	58_F	1.214	1.00
39_S	149_K	1.213	1.00
104_L	108_L	1.208	1.00
36_L	40_S	1.203	1.00
171_V	175_V	1.199	1.00
30_E	102_I	1.195	1.00
8_L	11_I	1.190	1.00
5_Y	8_L	1.163	1.00
9_G	13_G	1.149	1.00
51_I	108_L	1.148	1.00
9_G	132_I	1.146	1.00
88_R	91_M	1.132	1.00
26_M	106_M	1.125	1.00
46_M	141_L	1.124	1.00
35_W	149_K	1.123	1.00
44_G	100_Y	1.123	1.00
117_W	136_F	1.121	1.00
7_G	11_I	1.121	1.00
26_M	30_E	1.099	1.00
23_V	107_G	1.087	1.00
35_W	152_N	1.083	1.00
167_S	171_V	1.082	1.00
146_A	150_A	1.076	1.00
13_G	136_F	1.076	1.00
153_M	156_S	1.074	1.00
83_S	174_G	1.056	1.00
172_I	176_Y	1.049	1.00
27_I	144_I	1.048	1.00
73_I	178_L	1.048	1.00
46_M	137_F	1.045	1.00
17_A	140_I	1.041	1.00
89_A	163_K	1.029	1.00
24_N	144_I	1.027	1.00
39_S	145_I	1.022	1.00
27_I	31_S	1.017	1.00
8_L	12_L	1.012	1.00
40_S	99_N	1.002	1.00
23_V	140_I	0.994	1.00
164_V	168_V	0.993	1.00
153_M	157_K	0.989	1.00
49_D	107_G	0.965	1.00
32_M	151_V	0.957	1.00
78_F	81_Y	0.956	1.00
49_D	113_Q	0.939	1.00
91_M	94_K	0.934	1.00
23_V	106_M	0.934	1.00
35_W	145_I	0.914	1.00
12_L	15_S	0.912	1.00
25_A	29_N	0.910	1.00
10_I	14_L	0.906	1.00
14_L	139_G	0.900	1.00
52_F	114_I	0.898	1.00
47_T	104_L	0.896	1.00
6_L	138_V	0.890	1.00
23_V	49_D	0.889	1.00
165_I	168_V	0.889	1.00
37_H	40_S	0.881	1.00
175_V	179_Y	0.872	1.00
48_A	104_L	0.867	1.00
119_T	123_S	0.855	1.00
105_S	109_T	0.852	1.00
130_I	133_I	0.849	1.00
27_I	148_P	0.837	1.00
99_N	102_I	0.836	1.00
25_A	166_S	0.833	1.00
35_W	148_P	0.833	1.00
49_D	52_F	0.827	1.00
24_N	143_W	0.826	1.00
33_K	99_N	0.822	1.00
145_I	149_K	0.818	1.00
13_G	17_A	0.818	1.00
52_F	108_L	0.817	1.00
176_Y	179_Y	0.817	1.00
162_V	166_S	0.816	1.00
150_A	153_M	0.810	1.00
76_G	80_L	0.809	1.00
91_M	160_K	0.807	1.00
85_L	89_A	0.806	1.00
24_N	28_A	0.806	1.00
77_I	80_L	0.803	1.00
44_G	104_L	0.802	1.00
57_Y	129_S	0.793	1.00
141_L	145_I	0.781	1.00
88_R	92_P	0.775	1.00
67_I	70_A	0.764	1.00
47_T	51_I	0.764	1.00
61_G	64_P	0.762	1.00
11_I	15_S	0.759	1.00
86_T	167_S	0.756	1.00
15_S	19_P	0.753	1.00
34_S	37_H	0.752	1.00
53_F	117_W	0.749	1.00
171_V	174_G	0.745	1.00
60_Q	64_P	0.743	1.00
109_T	118_I	0.737	1.00
31_S	151_V	0.734	1.00
84_Y	171_V	0.731	1.00
58_F	61_G	0.730	1.00
74_I	77_I	0.727	1.00
81_Y	84_Y	0.724	1.00
87_I	171_V	0.721	1.00
42_G	46_M	0.720	1.00
77_I	81_Y	0.719	1.00
7_G	10_I	0.710	1.00
53_F	144_I	0.701	1.00
144_I	147_F	0.700	1.00
23_V	144_I	0.697	1.00
66_P	69_N	0.697	1.00
52_F	113_Q	0.696	1.00
39_S	148_P	0.691	1.00
45_A	49_D	0.687	1.00
165_I	169_I	0.685	1.00
6_L	10_I	0.684	1.00
42_G	145_I	0.682	1.00
149_K	153_M	0.682	1.00
51_I	54_I	0.676	1.00
74_I	109_T	0.676	1.00
41_I	99_N	0.674	1.00
154_L	157_K	0.674	1.00
24_N	106_M	0.668	1.00
27_I	45_A	0.663	1.00
46_M	140_I	0.659	1.00
28_A	147_F	0.659	1.00
90_K	160_K	0.657	1.00
52_F	118_I	0.656	1.00
93_S	96_I	0.656	1.00
49_D	137_F	0.656	1.00
107_G	113_Q	0.650	1.00
130_I	134_P	0.649	1.00
43_A	46_M	0.635	1.00
17_A	24_N	0.634	1.00
48_A	107_G	0.634	1.00
114_I	118_I	0.631	1.00
102_I	106_M	0.631	1.00
56_V	60_Q	0.630	1.00
94_K	97_K	0.629	1.00
131_L	135_G	0.627	1.00
53_F	137_F	0.626	1.00
70_A	73_I	0.622	1.00
39_S	42_G	0.619	1.00
49_D	140_I	0.615	1.00
52_F	56_V	0.613	1.00
83_S	87_I	0.610	1.00
75_G	115_S	0.607	1.00
138_V	142_I	0.607	1.00
151_V	154_L	0.606	1.00
45_A	107_G	0.604	1.00
49_D	117_W	0.601	1.00
27_I	41_I	0.598	1.00
143_W	146_A	0.597	1.00
56_V	117_W	0.594	1.00
17_A	144_I	0.592	1.00
17_A	143_W	0.590	1.00
20_P	112_Y	0.589	1.00
19_P	24_N	0.585	1.00
134_P	138_V	0.584	1.00
49_D	136_F	0.584	1.00
52_F	107_G	0.583	1.00
12_L	16_M	0.581	1.00
46_M	144_I	0.576	1.00
54_I	133_I	0.575	1.00
57_Y	133_I	0.575	1.00
41_I	106_M	0.573	1.00
148_P	152_N	0.573	1.00
22_P	110_N	0.570	1.00
107_G	144_I	0.570	1.00
143_W	148_P	0.566	1.00
14_L	18_A	0.565	1.00
147_F	151_V	0.562	1.00
17_A	49_D	0.561	1.00
93_S	97_K	0.561	1.00
29_N	163_K	0.559	1.00
168_V	171_V	0.554	1.00
76_G	171_V	0.554	1.00
156_S	159_V	0.551	1.00
38_G	41_I	0.551	1.00
94_K	98_G	0.547	1.00
87_I	168_V	0.547	1.00
91_M	163_K	0.541	1.00
125_I	129_S	0.539	1.00
137_F	141_L	0.538	1.00
35_W	153_M	0.537	1.00
166_S	170_L	0.534	0.99
63_I	71_L	0.529	0.99
9_G	122_I	0.529	0.99
117_W	140_I	0.526	0.99
22_P	86_T	0.524	0.99
136_F	140_I	0.523	0.99
50_L	54_I	0.522	0.99
150_A	154_L	0.521	0.99
172_I	175_V	0.518	0.99
56_V	125_I	0.518	0.99
66_P	71_L	0.516	0.99
19_P	163_K	0.515	0.99
56_V	114_I	0.510	0.99
142_I	146_A	0.507	0.99
23_V	113_Q	0.507	0.99
44_G	103_G	0.506	0.99
142_I	145_I	0.504	0.99
87_I	91_M	0.502	0.99
16_M	116_W	0.501	0.99
113_Q	140_I	0.501	0.99
146_A	149_K	0.501	0.99
45_A	113_Q	0.500	0.99
95_S	98_G	0.500	0.99

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3j1zP	8	0.5161	19.3	0.926	Contact Map
2cfqA	1	0.957	8.1	0.938	Contact Map
4uvmA	1	0.4839	7.4	0.939	Contact Map
4m64A	1	0.9785	7.1	0.939	Contact Map
2gfpA	2	0.9462	6.7	0.94	Contact Map
3wdoA	1	0.5054	5.8	0.942	Contact Map
4p6vD	1	0.4355	4.4	0.945	Contact Map
4q65A	1	0.4785	4.2	0.945	Contact Map
3h90A	3	0.4677	3	0.949	Contact Map
4g1uA	2	0.414	2.6	0.951	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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