Q97ZT7 - SULSO - GREMLIN Contacts

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Q97ZT7 - Phosphate transport system permease protein (PstC)

UniProt:	Q97ZT7	Sequences: MITIKRKREKFFYFLLIPSSVVVFTFILILSVLVYNSIPILEKVGLSIYTKNIWNPIYDQYGGLAAIYGTIVTSVLSVMISIPFATAFSIFVNDISPKKVKSFLVNLSDLLAAFPTVLYGFWGLLSLGPFLSRTLFTFLSSHVGLHQTTNGPSILLASIILAIMITPFASSLIREVYFQVPRSFEEAVYSLGLGKWELITLKLSYIKKAFLGAYALAFGRAVGETVAVSLTIGNVLNISPNLFEPGYTISSLIISQFGTAYGIQYNAMFALALFLVVIGFIFVIISKLIVRMRS	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	294 (249)
Sequences:	69660 (45445)
Seq/√Len:	2880.0

ECOLI - POTH PSTC YCJO THIP PSTA UGPA YCJP YDCU UGPE MALG CYST POTI POTB MALF YNJC YDCV CYSW POTC MODB TAUC YEHY SSUC PHNE YEHW METI PROW YECS
BACSU - GANP MDXF GANQ YURN YTEP MDXG AMYD LPLB ARAQ YQGH ARAP YESQ AMYC YQGI YESP YURM YTCP LPLC YVGM YTLD SSUC OPUAB METP TCYB YXEN TCYL OPUBD OPUCB OPUBB OPUCD
HALSA - Q9HML6 Q9HMT4 Q9HHQ7 Q9HP01 Q9HP02 Q9HR05 Q9HHQ6 Q9HS11 Q9HPK3 Q9HNI9 Q9HS12 Q9HML7
SULSO - Q97ZT7 Q97YY2 Q97ZT6 Q97UZ0 Q97UF3 Q97YY3 Q97UF4 Q97UY9 Q97ZC2 Q97ZC1 Q97VC0 Q97Z98

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
197_E	200_T	3.911	1.00
68_Y	72_V	3.209	1.00
29_I	32_V	3.028	1.00
189_Y	198_L	3.014	1.00
174_R	178_F	2.981	1.00
65_A	68_Y	2.590	1.00
94_D	185_E	2.575	1.00
185_E	202_K	2.568	1.00
188_V	198_L	2.536	1.00
194_G	197_E	2.513	1.00
66_A	252_L	2.486	1.00
76_L	158_S	2.441	1.00
176_V	209_A	2.405	1.00
37_S	54_W	2.397	1.00
52_N	55_N	2.369	1.00
78_V	214_Y	2.318	1.00
64_L	68_Y	2.316	1.00
247_Y	255_S	2.279	1.00
195_K	198_L	2.275	1.00
173_I	210_F	2.253	1.00
52_N	56_P	2.249	1.00
264_Q	267_A	2.104	1.00
193_L	197_E	2.095	1.00
86_T	199_I	2.042	1.00
13_Y	18_P	2.031	1.00
55_N	59_D	2.027	1.00
80_I	162_A	2.025	1.00
98_K	101_K	1.975	1.00
31_S	35_Y	1.956	1.00
169_A	213_A	1.931	1.00
184_F	205_Y	1.927	1.00
93_N	178_F	1.906	1.00
177_Y	206_I	1.892	1.00
188_V	193_L	1.837	1.00
35_Y	39_P	1.797	1.00
86_T	206_I	1.790	1.00
72_V	154_I	1.789	1.00
189_Y	195_K	1.748	1.00
82_I	214_Y	1.747	1.00
284_I	288_L	1.742	1.00
65_A	69_G	1.730	1.00
15_L	18_P	1.719	1.00
105_V	174_R	1.694	1.00
185_E	198_L	1.687	1.00
74_S	218_F	1.684	1.00
14_F	18_P	1.672	1.00
85_A	169_A	1.667	1.00
77_S	221_A	1.622	1.00
14_F	17_I	1.614	1.00
72_V	76_L	1.602	1.00
92_V	170_S	1.589	1.00
28_L	32_V	1.586	1.00
86_T	173_I	1.584	1.00
17_I	20_S	1.563	1.00
185_E	189_Y	1.552	1.00
88_F	170_S	1.551	1.00
56_P	267_A	1.548	1.00
32_V	37_S	1.544	1.00
252_L	256_Q	1.541	1.00
32_V	36_N	1.478	1.00
169_A	217_A	1.402	1.00
203_L	210_F	1.397	1.00
182_R	185_E	1.393	1.00
100_V	103_F	1.391	1.00
212_G	216_L	1.373	1.00
225_T	229_S	1.372	1.00
93_N	174_R	1.362	1.00
177_Y	202_K	1.353	1.00
12_F	16_L	1.352	1.00
89_S	173_I	1.347	1.00
81_S	217_A	1.336	1.00
62_G	65_A	1.330	1.00
29_I	41_L	1.322	1.00
227_A	231_T	1.320	1.00
173_I	213_A	1.314	1.00
97_P	101_K	1.288	1.00
95_I	98_K	1.285	1.00
83_P	87_A	1.281	1.00
51_K	55_N	1.269	1.00
94_D	182_R	1.244	1.00
153_S	246_G	1.241	1.00
188_V	201_L	1.236	1.00
248_T	251_S	1.236	1.00
73_T	248_T	1.232	1.00
256_Q	264_Q	1.224	1.00
182_R	186_E	1.218	1.00
90_I	177_Y	1.207	1.00
225_T	254_I	1.202	1.00
50_T	53_I	1.189	1.00
86_T	210_F	1.185	1.00
56_P	59_D	1.181	1.00
70_T	249_I	1.176	1.00
34_V	273_L	1.175	1.00
54_W	273_L	1.172	1.00
183_S	186_E	1.172	1.00
247_Y	252_L	1.167	1.00
40_I	43_K	1.160	1.00
76_L	79_M	1.154	1.00
160_I	231_T	1.150	1.00
180_V	205_Y	1.132	1.00
127_L	156_L	1.129	1.00
194_G	198_L	1.119	1.00
103_F	106_N	1.102	1.00
79_M	83_P	1.096	1.00
207_K	211_L	1.094	1.00
69_G	72_V	1.088	1.00
152_P	155_L	1.072	1.00
39_P	43_K	1.070	1.00
82_I	210_F	1.065	1.00
103_F	107_L	1.064	1.00
157_A	228_V	1.062	1.00
166_T	170_S	1.056	1.00
36_N	39_P	1.054	1.00
29_I	37_S	1.046	1.00
173_I	209_A	1.046	1.00
185_E	195_K	1.032	1.00
154_I	246_G	1.026	1.00
77_S	81_S	1.018	1.00
43_K	48_I	1.018	1.00
90_I	199_I	1.014	1.00
13_Y	17_I	1.006	1.00
58_Y	64_L	1.000	1.00
205_Y	208_K	0.993	1.00
155_L	159_I	0.992	1.00
247_Y	251_S	0.990	1.00
80_I	158_S	0.990	1.00
71_I	75_V	0.988	1.00
77_S	161_L	0.985	1.00
53_I	266_N	0.982	1.00
255_S	259_T	0.982	1.00
224_E	227_A	0.978	1.00
76_L	80_I	0.976	1.00
87_A	91_F	0.974	1.00
252_L	271_L	0.972	1.00
93_N	177_Y	0.970	1.00
172_L	213_A	0.967	1.00
12_F	18_P	0.963	1.00
161_L	221_A	0.962	1.00
13_Y	16_L	0.961	1.00
224_E	228_V	0.958	1.00
101_K	178_F	0.954	1.00
90_I	195_K	0.949	1.00
193_L	198_L	0.949	1.00
33_L	273_L	0.939	1.00
125_L	129_P	0.935	1.00
252_L	255_S	0.916	1.00
32_V	35_Y	0.915	1.00
67_I	249_I	0.911	1.00
36_N	269_F	0.909	1.00
228_V	248_T	0.900	1.00
75_V	79_M	0.899	1.00
23_V	27_I	0.891	1.00
56_P	60_Q	0.886	1.00
72_V	75_V	0.886	1.00
151_G	155_L	0.883	1.00
257_F	268_M	0.882	1.00
123_G	231_T	0.881	1.00
60_Q	64_L	0.875	1.00
63_G	271_L	0.874	1.00
89_S	93_N	0.870	1.00
123_G	160_I	0.868	1.00
176_V	206_I	0.867	1.00
99_K	102_S	0.866	1.00
77_S	158_S	0.865	1.00
285_I	288_L	0.864	1.00
207_K	210_F	0.864	1.00
73_T	154_I	0.860	1.00
154_I	158_S	0.858	1.00
25_T	29_I	0.856	1.00
79_M	214_Y	0.852	1.00
67_I	71_I	0.847	1.00
285_I	289_I	0.845	1.00
206_I	209_A	0.842	1.00
102_S	105_V	0.841	1.00
80_I	83_P	0.841	1.00
169_A	173_I	0.841	1.00
69_G	154_I	0.840	1.00
109_D	171_S	0.837	1.00
72_V	155_L	0.828	1.00
81_S	85_A	0.824	1.00
158_S	162_A	0.824	1.00
57_I	274_F	0.824	1.00
199_I	203_L	0.823	1.00
81_S	165_I	0.820	1.00
254_I	258_G	0.820	1.00
217_A	221_A	0.817	1.00
173_I	206_I	0.815	1.00
91_F	98_K	0.811	1.00
73_T	161_L	0.811	1.00
193_L	200_T	0.809	1.00
32_V	40_I	0.808	1.00
229_S	251_S	0.807	1.00
151_G	154_I	0.805	1.00
85_A	170_S	0.805	1.00
116_T	164_M	0.800	1.00
152_P	234_N	0.796	1.00
120_G	226_V	0.796	1.00
128_G	132_S	0.793	1.00
150_N	153_S	0.792	1.00
15_L	19_S	0.785	1.00
256_Q	267_A	0.784	1.00
256_Q	262_G	0.782	1.00
198_L	202_K	0.782	1.00
195_K	199_I	0.780	1.00
89_S	177_Y	0.779	1.00
37_S	40_I	0.777	1.00
32_V	41_L	0.776	1.00
153_S	156_L	0.771	1.00
281_I	285_I	0.770	1.00
92_V	105_V	0.766	1.00
68_Y	71_I	0.759	1.00
232_I	246_G	0.753	1.00
180_V	206_I	0.750	1.00
279_G	283_V	0.745	1.00
89_S	170_S	0.739	1.00
14_F	20_S	0.737	1.00
109_D	112_A	0.731	1.00
129_P	132_S	0.729	1.00
199_I	202_K	0.727	1.00
74_S	78_V	0.727	1.00
66_A	69_G	0.726	1.00
160_I	227_A	0.726	1.00
267_A	271_L	0.725	1.00
12_F	15_L	0.725	1.00
56_P	264_Q	0.724	1.00
184_F	201_L	0.723	1.00
124_L	128_G	0.723	1.00
28_L	31_S	0.720	1.00
256_Q	271_L	0.717	1.00
27_I	30_L	0.711	1.00
82_I	173_I	0.711	1.00
269_F	273_L	0.707	1.00
38_I	49_Y	0.700	1.00
92_V	104_L	0.698	1.00
33_L	269_F	0.697	1.00
33_L	272_A	0.695	1.00
120_G	230_L	0.695	1.00
23_V	26_F	0.691	1.00
78_V	218_F	0.688	1.00
85_A	173_I	0.687	1.00
132_S	155_L	0.685	1.00
182_R	189_Y	0.684	1.00
235_V	247_Y	0.684	1.00
173_I	177_Y	0.684	1.00
250_S	275_L	0.684	1.00
232_I	236_L	0.683	1.00
81_S	169_A	0.681	1.00
48_I	51_K	0.680	1.00
33_L	36_N	0.680	1.00
43_K	47_S	0.679	1.00
16_L	20_S	0.676	1.00
128_G	151_G	0.674	1.00
152_P	156_L	0.667	1.00
40_I	47_S	0.663	1.00
21_V	25_T	0.663	1.00
187_A	191_L	0.663	1.00
78_V	82_I	0.663	1.00
128_G	131_L	0.660	1.00
210_F	213_A	0.660	1.00
156_L	160_I	0.659	1.00
112_A	167_P	0.658	1.00
265_Y	268_M	0.657	1.00
54_W	270_A	0.656	1.00
155_L	158_S	0.655	1.00
70_T	250_S	0.654	1.00
224_E	248_T	0.648	1.00
92_V	174_R	0.647	1.00
157_A	161_L	0.638	1.00
21_V	24_F	0.637	1.00
57_I	267_A	0.637	1.00
64_L	67_I	0.637	1.00
116_T	226_V	0.636	1.00
253_I	271_L	0.635	1.00
73_T	157_A	0.629	1.00
232_I	247_Y	0.629	1.00
202_K	205_Y	0.628	1.00
66_A	249_I	0.627	1.00
55_N	58_Y	0.624	1.00
81_S	221_A	0.623	1.00
88_F	166_T	0.623	1.00
251_S	255_S	0.622	1.00
172_L	209_A	0.620	1.00
30_L	33_L	0.619	1.00
36_N	41_L	0.617	1.00
49_Y	52_N	0.616	1.00
40_I	48_I	0.614	1.00
209_A	213_A	0.614	1.00
70_T	222_V	0.613	1.00
210_F	214_Y	0.613	1.00
98_K	104_L	0.608	1.00
61_Y	65_A	0.607	1.00
84_F	162_A	0.607	1.00
29_I	40_I	0.604	1.00
259_T	262_G	0.604	1.00
61_Y	64_L	0.603	1.00
105_V	171_S	0.602	1.00
88_F	108_S	0.602	1.00
156_L	231_T	0.601	1.00
77_S	162_A	0.600	1.00
165_I	217_A	0.596	1.00
37_S	273_L	0.595	1.00
266_N	269_F	0.595	1.00
22_V	26_F	0.590	1.00
187_A	190_S	0.588	1.00
43_K	46_L	0.587	1.00
81_S	162_A	0.587	1.00
95_I	101_K	0.585	1.00
36_N	40_I	0.585	1.00
278_I	281_I	0.584	1.00
157_A	232_I	0.584	1.00
269_F	272_A	0.582	1.00
91_F	95_I	0.581	1.00
249_I	275_L	0.581	1.00
198_L	201_L	0.580	1.00
228_V	251_S	0.572	1.00
251_S	254_I	0.572	1.00
39_P	42_E	0.571	1.00
73_T	158_S	0.570	1.00
275_L	279_G	0.570	1.00
76_L	162_A	0.570	1.00
123_G	126_S	0.570	1.00
100_V	104_L	0.569	1.00
37_S	41_L	0.569	1.00
112_A	168_F	0.569	1.00
38_I	50_T	0.568	1.00
265_Y	269_F	0.568	1.00
57_I	271_L	0.564	1.00
12_F	17_I	0.560	1.00
42_E	45_G	0.560	1.00
280_F	284_I	0.559	1.00
74_S	221_A	0.558	1.00
16_L	19_S	0.550	1.00
165_I	220_R	0.550	1.00
263_I	267_A	0.549	1.00
236_L	246_G	0.549	1.00
226_V	230_L	0.548	1.00
120_G	124_L	0.543	1.00
283_V	287_K	0.539	1.00
125_L	128_G	0.538	1.00
119_Y	163_I	0.536	1.00
219_G	223_G	0.534	1.00
267_A	270_A	0.534	1.00
226_V	229_S	0.533	1.00
272_A	275_L	0.531	1.00
35_Y	38_I	0.530	1.00
117_V	230_L	0.528	1.00
161_L	228_V	0.524	1.00
78_V	221_A	0.523	1.00
89_S	174_R	0.522	1.00
74_S	222_V	0.521	1.00
24_F	28_L	0.519	1.00
15_L	20_S	0.519	1.00
211_L	214_Y	0.517	1.00
102_S	106_N	0.517	1.00
220_R	223_G	0.516	1.00
73_T	77_S	0.512	1.00
124_L	151_G	0.511	1.00
90_I	202_K	0.511	1.00
53_I	56_P	0.510	1.00
108_S	167_P	0.510	1.00
29_I	38_I	0.510	1.00
99_K	103_F	0.509	1.00
82_I	86_T	0.509	1.00
180_V	185_E	0.509	1.00
62_G	247_Y	0.508	1.00
114_F	118_L	0.507	1.00
84_F	166_T	0.507	1.00
107_L	111_L	0.504	1.00
131_L	150_N	0.502	1.00
88_F	91_F	0.502	1.00
32_V	39_P	0.502	1.00
171_S	175_E	0.502	1.00
104_L	108_S	0.500	1.00
132_S	150_N	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4tquM	1	0.9354	100	0.332	Contact Map
2onkC	2	0.8333	100	0.335	Contact Map
3d31C	2	0.8401	100	0.339	Contact Map
3rlfG	1	0.8844	100	0.377	Contact Map
3rlfF	1	0.9456	100	0.38	Contact Map
4tquN	1	0.8844	100	0.39	Contact Map
4ymuD	2	0.7245	99.9	0.512	Contact Map
3tuiA	2	0.7075	99.9	0.538	Contact Map
2m8rA	1	0.1259	4.8	0.955	Contact Map
2k9pA	1	0.1633	4	0.957	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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