Q97ZT6 - SULSO - GREMLIN Contacts

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Q97ZT6 - Phosphate transport system permease protein (PstA)

UniProt:	Q97ZT6	Sequences: MRRKDTVIVSLITLLVILFLLPITFLLYTVVVNGSKVIISYGPRFFIDLPPNPGEGLGGIGPALEGSLYMVSLAVLISAPISIFTGVFLSFFERSKIASLIRASLELMVEFPTIVIGIVIFTILVVEIGLGLNGITAAMALAIVMLPYSTIQISEALRIPKKLYEEVAYSLGLTTWQVIKLVMYIGRRGIINGLLIGFAKIIGETAPIIFLTSSTANLYIKTFNTAVTGIPVMIYNYAFSGYSNLNEVAWGASFVLIILVTIIFVVVRKMAK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	272 (246)
Sequences:	69595 (44533)
Seq/√Len:	2839.3

ECOLI - YCJP UGPE PSTC POTH POTI UGPA MALG PSTA YDCU YCJO YDCV THIP POTB POTC YNJC MALF CYST CYSW MODB TAUC PHNE SSUC YEHW YEHY METI PROW YECS GLNP
BACSU - AMYC GANP MDXG GANQ YQGH YESQ MDXF AMYD YURM YURN ARAP YTCP YQGI ARAQ YTEP LPLB LPLC YESP YVGM SSUC YTLD OPUAB TCYB METP YXEN TCYL OPUBD OPUCB OPUBB OPUCD ARTQ
HALSA - Q9HML6 Q9HP02 Q9HMT4 Q9HP01 Q9HHQ7 Q9HS11 Q9HHQ6 Q9HR05 Q9HPK3 Q9HNI9 Q9HS12 Q9HML7
SULSO - Q97ZT6 Q97ZT7 Q97YY2 Q97UF3 Q97YY3 Q97UY9 Q97ZC1 Q97ZC2 Q97UZ0 Q97UF4 Q97VC0 Q97Z98

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
177_Q	180_K	3.875	1.00
65_E	69_Y	3.278	1.00
169_Y	178_V	3.158	1.00
154_S	158_R	3.071	1.00
62_P	65_E	2.632	1.00
63_A	233_M	2.607	1.00
91_F	165_E	2.569	1.00
174_T	177_Q	2.560	1.00
34_G	51_P	2.540	1.00
168_A	178_V	2.535	1.00
156_A	189_G	2.508	1.00
165_E	182_V	2.495	1.00
73_L	138_A	2.446	1.00
49_L	52_N	2.422	1.00
228_T	236_N	2.420	1.00
175_T	178_V	2.365	1.00
61_G	65_E	2.354	1.00
75_V	194_L	2.347	1.00
49_L	53_P	2.220	1.00
52_N	56_G	2.218	1.00
153_I	190_I	2.214	1.00
95_S	98_A	2.147	1.00
77_I	142_A	2.127	1.00
173_L	177_Q	2.077	1.00
83_I	179_I	1.991	1.00
90_S	158_R	1.950	1.00
149_S	193_G	1.948	1.00
69_Y	134_G	1.939	1.00
157_L	186_G	1.937	1.00
164_Y	185_I	1.922	1.00
28_Y	32_V	1.888	1.00
32_V	36_K	1.881	1.00
168_A	173_L	1.868	1.00
83_I	186_G	1.811	1.00
102_R	154_S	1.799	1.00
79_A	194_L	1.784	1.00
62_P	66_G	1.781	1.00
169_Y	175_T	1.768	1.00
165_E	178_V	1.759	1.00
245_L	248_V	1.754	1.00
71_V	198_F	1.717	1.00
82_S	149_S	1.674	1.00
69_Y	73_L	1.673	1.00
74_A	201_I	1.667	1.00
53_P	248_V	1.626	1.00
89_L	150_T	1.626	1.00
83_I	153_I	1.619	1.00
165_E	169_Y	1.612	1.00
85_T	150_T	1.577	1.00
13_T	17_I	1.506	1.00
134_G	227_V	1.494	1.00
233_M	237_Y	1.492	1.00
162_K	165_E	1.467	1.00
97_I	100_L	1.437	1.00
205_T	209_I	1.420	1.00
149_S	197_G	1.416	1.00
86_G	153_I	1.398	1.00
25_F	29_T	1.397	1.00
183_M	190_I	1.394	1.00
59_G	62_P	1.392	1.00
31_V	254_F	1.388	1.00
80_P	84_F	1.386	1.00
22_P	26_L	1.377	1.00
90_S	154_S	1.374	1.00
92_F	95_S	1.372	1.00
78_S	197_G	1.358	1.00
192_N	196_I	1.356	1.00
70_M	229_G	1.353	1.00
157_L	182_V	1.342	1.00
29_T	33_N	1.329	1.00
215_T	226_A	1.323	1.00
48_D	52_N	1.319	1.00
53_P	56_G	1.315	1.00
153_I	193_G	1.314	1.00
214_S	226_A	1.289	1.00
207_P	211_L	1.282	1.00
162_K	166_E	1.282	1.00
91_F	162_K	1.277	1.00
94_R	98_A	1.269	1.00
168_A	181_L	1.266	1.00
83_I	190_I	1.259	1.00
205_T	235_Y	1.259	1.00
163_L	166_E	1.259	1.00
130_L	135_I	1.237	1.00
47_I	50_P	1.216	1.00
100_L	103_A	1.207	1.00
229_G	232_V	1.200	1.00
73_L	76_L	1.190	1.00
87_V	157_L	1.189	1.00
17_I	21_L	1.189	1.00
66_G	69_Y	1.189	1.00
67_S	230_I	1.188	1.00
124_L	136_T	1.183	1.00
228_T	233_M	1.164	1.00
40_S	45_F	1.160	1.00
140_A	211_L	1.157	1.00
174_T	178_V	1.156	1.00
212_T	227_V	1.156	1.00
160_P	185_I	1.154	1.00
76_L	80_P	1.149	1.00
237_Y	245_L	1.149	1.00
237_Y	241_G	1.149	1.00
29_T	32_V	1.131	1.00
187_R	191_I	1.128	1.00
130_L	133_N	1.119	1.00
79_A	190_I	1.114	1.00
132_L	135_I	1.114	1.00
30_V	254_F	1.090	1.00
133_N	227_V	1.084	1.00
10_S	14_L	1.080	1.00
165_E	175_T	1.079	1.00
228_T	232_V	1.073	1.00
100_L	104_S	1.071	1.00
146_L	150_T	1.066	1.00
153_I	189_G	1.057	1.00
236_N	240_S	1.056	1.00
90_S	157_L	1.048	1.00
53_P	57_L	1.043	1.00
77_I	138_A	1.032	1.00
74_A	78_S	1.031	1.00
152_Q	193_G	1.019	1.00
137_A	208_I	1.015	1.00
39_I	42_G	1.012	1.00
10_S	13_T	1.011	1.00
84_F	88_F	1.005	1.00
135_I	139_M	1.000	1.00
185_I	188_R	1.000	1.00
68_L	72_S	0.998	1.00
87_V	179_I	0.990	1.00
173_L	178_V	0.984	1.00
204_E	207_P	0.981	1.00
87_V	175_T	0.978	1.00
37_V	40_S	0.962	1.00
73_L	77_I	0.956	1.00
64_L	230_I	0.956	1.00
51_P	254_F	0.946	1.00
74_A	141_L	0.943	1.00
204_E	208_I	0.942	1.00
50_P	247_E	0.939	1.00
55_E	61_G	0.939	1.00
122_T	126_V	0.933	1.00
134_G	138_A	0.931	1.00
76_L	194_L	0.928	1.00
141_L	201_I	0.926	1.00
120_I	211_L	0.917	1.00
98_A	158_R	0.916	1.00
20_L	24_T	0.913	1.00
233_M	252_A	0.908	1.00
9_V	13_T	0.905	1.00
233_M	236_N	0.904	1.00
69_Y	135_I	0.903	1.00
208_I	229_G	0.902	1.00
69_Y	72_S	0.900	1.00
86_G	90_S	0.897	1.00
70_M	134_G	0.887	1.00
131_G	135_I	0.883	1.00
33_N	250_W	0.879	1.00
235_Y	239_F	0.875	1.00
82_S	150_T	0.874	1.00
72_S	76_L	0.873	1.00
214_S	227_V	0.868	1.00
12_I	16_V	0.867	1.00
238_A	249_A	0.864	1.00
64_L	68_L	0.862	1.00
99_S	102_R	0.860	1.00
77_I	80_P	0.858	1.00
186_G	189_G	0.856	1.00
96_K	99_S	0.854	1.00
153_I	186_G	0.849	1.00
78_S	82_S	0.847	1.00
187_R	190_I	0.846	1.00
74_A	138_A	0.846	1.00
156_A	186_G	0.841	1.00
131_G	134_G	0.841	1.00
106_E	151_I	0.840	1.00
209_I	232_V	0.840	1.00
36_K	40_S	0.837	1.00
140_A	207_P	0.835	1.00
173_L	180_K	0.825	1.00
138_A	142_A	0.824	1.00
53_P	245_L	0.821	1.00
88_F	95_S	0.820	1.00
89_L	102_R	0.819	1.00
178_V	182_V	0.819	1.00
78_S	145_M	0.819	1.00
66_G	134_G	0.816	1.00
117_G	206_A	0.815	1.00
121_F	125_V	0.813	1.00
86_G	157_L	0.809	1.00
35_S	46_F	0.808	1.00
65_E	68_L	0.807	1.00
135_I	138_A	0.805	1.00
197_G	201_I	0.802	1.00
113_T	144_V	0.799	1.00
86_G	150_T	0.798	1.00
70_M	141_L	0.797	1.00
179_I	183_M	0.795	1.00
149_S	153_I	0.793	1.00
175_T	179_I	0.790	1.00
120_I	140_A	0.789	1.00
71_V	75_V	0.788	1.00
60_I	252_A	0.787	1.00
132_L	214_S	0.775	1.00
237_Y	248_V	0.761	1.00
33_N	36_K	0.755	1.00
30_V	250_W	0.748	1.00
37_V	44_R	0.747	1.00
164_Y	181_L	0.745	1.00
125_V	131_G	0.739	1.00
179_I	182_V	0.733	1.00
14_L	17_I	0.732	1.00
48_D	51_P	0.731	1.00
133_N	136_T	0.730	1.00
78_S	149_S	0.729	1.00
215_T	228_T	0.728	1.00
232_V	236_N	0.728	1.00
237_Y	252_A	0.728	1.00
262_I	266_V	0.727	1.00
20_L	23_I	0.719	1.00
79_A	153_I	0.718	1.00
260_V	264_F	0.718	1.00
46_F	49_L	0.715	1.00
82_S	153_I	0.713	1.00
167_V	171_L	0.713	1.00
132_L	136_T	0.712	1.00
63_A	230_I	0.708	1.00
78_S	201_I	0.708	1.00
106_E	109_V	0.708	1.00
89_L	101_I	0.706	1.00
256_L	260_V	0.705	1.00
265_V	268_R	0.704	1.00
89_L	154_S	0.702	1.00
117_G	210_F	0.700	1.00
37_V	45_F	0.699	1.00
58_G	62_P	0.699	1.00
75_V	198_F	0.697	1.00
247_E	250_W	0.693	1.00
248_V	252_A	0.692	1.00
30_V	253_S	0.692	1.00
242_Y	245_L	0.689	1.00
250_W	254_F	0.688	1.00
25_F	28_Y	0.686	1.00
153_I	157_L	0.686	1.00
63_A	66_G	0.685	1.00
234_I	252_A	0.685	1.00
75_V	79_A	0.684	1.00
40_S	44_R	0.682	1.00
162_K	169_Y	0.681	1.00
125_V	128_I	0.680	1.00
61_G	64_L	0.677	1.00
54_G	255_V	0.676	1.00
45_F	48_D	0.673	1.00
190_I	193_G	0.673	1.00
160_P	186_G	0.669	1.00
204_E	229_G	0.667	1.00
70_M	137_A	0.666	1.00
95_S	101_I	0.665	1.00
19_F	23_I	0.665	1.00
190_I	194_L	0.663	1.00
33_N	37_V	0.662	1.00
231_P	256_L	0.660	1.00
88_F	92_F	0.660	1.00
167_V	170_S	0.659	1.00
92_F	98_A	0.659	1.00
85_T	105_L	0.658	1.00
85_T	146_L	0.656	1.00
35_S	47_I	0.655	1.00
113_T	206_A	0.655	1.00
11_L	15_L	0.651	1.00
264_F	268_R	0.641	1.00
136_T	140_A	0.639	1.00
189_G	193_G	0.638	1.00
11_L	14_L	0.638	1.00
246_N	250_W	0.638	1.00
54_G	248_V	0.634	1.00
57_L	61_G	0.632	1.00
102_R	151_I	0.631	1.00
137_A	141_L	0.630	1.00
58_G	61_G	0.628	1.00
121_F	131_G	0.624	1.00
182_V	185_I	0.624	1.00
67_S	231_P	0.621	1.00
137_A	212_T	0.619	1.00
230_I	256_L	0.618	1.00
70_M	138_A	0.614	1.00
81_I	142_A	0.614	1.00
67_S	202_I	0.612	1.00
50_P	53_P	0.612	1.00
122_T	125_V	0.608	1.00
97_I	101_I	0.602	1.00
120_I	123_I	0.598	1.00
212_T	228_T	0.593	1.00
78_S	142_A	0.591	1.00
96_K	100_L	0.591	1.00
34_G	251_G	0.590	1.00
194_L	197_G	0.588	1.00
9_V	12_I	0.584	1.00
152_Q	189_G	0.584	1.00
87_V	182_V	0.584	1.00
116_I	143_I	0.582	1.00
232_V	235_Y	0.582	1.00
74_A	142_A	0.581	1.00
38_I	42_G	0.581	1.00
206_A	209_I	0.580	1.00
52_N	55_E	0.580	1.00
145_M	197_G	0.578	1.00
16_V	20_L	0.574	1.00
178_V	181_L	0.573	1.00
105_L	147_P	0.572	1.00
246_N	249_A	0.570	1.00
199_A	203_G	0.568	1.00
109_V	147_P	0.568	1.00
36_K	44_R	0.567	1.00
114_I	210_F	0.566	1.00
136_T	211_L	0.565	1.00
71_V	201_I	0.564	1.00
51_P	251_G	0.562	1.00
99_S	103_A	0.560	1.00
145_M	200_K	0.558	1.00
71_V	202_I	0.553	1.00
191_I	194_L	0.552	1.00
109_V	148_Y	0.552	1.00
75_V	201_I	0.551	1.00
259_L	263_I	0.551	1.00
113_T	117_G	0.550	1.00
13_T	16_V	0.549	1.00
117_G	121_F	0.548	1.00
241_G	245_L	0.546	1.00
250_W	253_S	0.546	1.00
24_T	28_Y	0.545	1.00
259_L	262_I	0.544	1.00
86_G	154_S	0.544	1.00
81_I	146_L	0.543	1.00
21_L	25_F	0.543	1.00
36_K	45_F	0.542	1.00
141_L	208_I	0.542	1.00
34_G	37_V	0.541	1.00
66_G	227_V	0.539	1.00
206_A	210_F	0.536	1.00
248_V	251_G	0.531	1.00
34_G	38_I	0.531	1.00
261_T	265_V	0.530	1.00
70_M	74_A	0.528	1.00
103_A	107_L	0.526	1.00
130_L	139_M	0.526	1.00
126_V	129_G	0.524	1.00
200_K	203_G	0.521	1.00
79_A	83_I	0.521	1.00
83_I	183_M	0.521	1.00
166_E	169_Y	0.520	1.00
253_S	256_L	0.520	1.00
59_G	233_M	0.519	1.00
111_F	115_V	0.518	1.00
15_L	19_F	0.516	1.00
62_P	69_Y	0.513	1.00
102_R	158_R	0.513	1.00
73_L	142_A	0.510	1.00
85_T	88_F	0.509	1.00
160_P	165_E	0.508	1.00
75_V	80_P	0.507	1.00
32_V	46_F	0.507	1.00
104_S	108_M	0.507	1.00
47_I	51_P	0.505	1.00
59_G	228_T	0.505	1.00
101_I	105_L	0.504	1.00
31_V	35_S	0.503	1.00
201_I	204_E	0.502	1.00
108_M	147_P	0.501	1.00
14_L	18_L	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2onkC	2	0.886	100	0.284	Contact Map
4tquM	1	0.9301	100	0.301	Contact Map
3d31C	2	0.8676	100	0.308	Contact Map
4tquN	1	0.9412	100	0.314	Contact Map
3rlfG	1	0.9265	100	0.317	Contact Map
3rlfF	1	0.9559	100	0.353	Contact Map
4ymuD	2	0.7684	99.9	0.472	Contact Map
3tuiA	2	0.7537	99.9	0.489	Contact Map
3mktA	1	0.5368	9	0.946	Contact Map
3w4tA	1	0.5	5.7	0.95	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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