Q97ZS3 - SULSO - GREMLIN Contacts

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Q97ZS3 - Uncharacterized protein

UniProt:	Q97ZS3	Sequences: MERIVELEEQIKKERNKEKRNTLYISLIQEYHNLYKKYKNTEFLDRALSIANKIEKKNPTVLNILGLIYLEKRRPDIAILTFKELLSNYNLNNEDKNVILYNLSLAYFQKGELLNAYNTLKGLVLNSRGEINVLSKKLLVKVCLALGEIRYIEEARELLEEFNIPSEELAIAYAALAKYYNDNKLLKKAKDIAIVLGNEKILADILSISDDEEDLRKAIQIYEKLGDHNSELKPLYKLSNKDPSLFAQILEKIGNLEDSKDKLVILYDIYRRTKILQFLKEAIKTAEKLQDYLFLARAYSELANYEEEVTNLRKAISFYEKYIEKSQK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	328 (275)
Sequences:	4737 (4344)
Seq/√Len:	261.9

ECOLI - HEMY LPSRP PGAA YBEQ YPHG NLPI BCSC
BACSU - YVCD YPIA RAPE RAPJ RAPA RAPI RAPF RAPH RAPB RAPC RAPG RAPK RAPD YRRB
HALSA - Q9HMM8
SULSO - Q97ZS3 Q97VC4 Q97V36 Q97WM3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
74_R	77_I	6.410	1.00
112_E	115_N	6.374	1.00
36_K	39_K	5.736	1.00
148_E	154_E	5.144	1.00
311_N	314_K	4.560	1.00
214_D	217_K	4.376	1.00
278_F	281_E	4.240	1.00
185_L	188_K	3.992	1.00
245_L	248_Q	3.294	1.00
104_S	120_L	3.158	1.00
66_G	82_F	3.059	1.00
28_I	44_L	2.798	1.00
140_V	159_L	2.554	1.00
100_L	119_T	2.548	1.00
31_Y	39_K	2.453	1.00
45_D	65_L	2.433	1.00
147_G	152_I	2.226	1.00
69_Y	77_I	2.162	1.00
83_K	103_L	2.158	1.00
107_Y	119_T	2.126	1.00
101_Y	138_L	2.086	1.00
139_L	143_C	2.058	1.00
156_R	160_E	2.027	1.00
44_L	64_I	2.022	1.00
103_L	119_T	1.993	1.00
82_F	102_N	1.981	1.00
143_C	154_E	1.963	1.00
267_Y	298_A	1.945	1.00
104_S	119_T	1.942	1.00
28_I	47_A	1.925	1.00
117_Y	121_K	1.906	1.00
107_Y	115_N	1.890	1.00
27_L	31_Y	1.888	1.00
117_Y	143_C	1.801	1.00
136_K	158_L	1.792	1.00
120_L	138_L	1.768	1.00
41_T	45_D	1.718	1.00
66_G	85_L	1.716	1.00
65_L	81_T	1.702	1.00
180_Y	185_L	1.681	1.00
47_A	50_I	1.674	1.00
197_G	200_K	1.663	1.00
79_I	110_K	1.658	1.00
65_L	69_Y	1.643	1.00
101_Y	135_S	1.612	1.00
107_Y	112_E	1.581	1.00
62_L	81_T	1.570	1.00
104_S	123_L	1.565	1.00
63_N	102_N	1.562	1.00
188_K	191_D	1.555	1.00
69_Y	74_R	1.551	1.00
31_Y	36_K	1.551	1.00
269_I	273_T	1.544	1.00
103_L	107_Y	1.541	1.00
69_Y	81_T	1.516	1.00
79_I	83_K	1.514	1.00
143_C	158_L	1.501	1.00
62_L	85_L	1.496	1.00
143_C	148_E	1.491	1.00
28_I	43_F	1.451	1.00
31_Y	43_F	1.442	1.00
161_E	165_P	1.397	1.00
67_L	102_N	1.389	1.00
249_I	253_I	1.373	1.00
306_E	311_N	1.363	1.00
140_V	162_F	1.357	1.00
283_I	287_E	1.354	1.00
176_L	180_Y	1.330	1.00
235_L	239_S	1.328	1.00
117_Y	146_L	1.321	1.00
117_Y	139_L	1.307	1.00
83_K	115_N	1.304	1.00
41_T	65_L	1.300	1.00
273_T	281_E	1.292	1.00
299_Y	303_A	1.289	1.00
302_L	306_E	1.281	1.00
77_I	80_L	1.279	1.00
32_H	68_I	1.277	1.00
122_G	126_N	1.275	1.00
217_K	220_Q	1.270	1.00
39_K	42_E	1.268	1.00
263_L	270_Y	1.254	1.00
101_Y	105_L	1.237	1.00
41_T	72_K	1.236	1.00
219_I	223_E	1.234	1.00
121_K	139_L	1.223	1.00
41_T	69_Y	1.222	1.00
190_K	194_I	1.219	1.00
203_A	206_L	1.207	1.00
41_T	68_I	1.193	1.00
306_E	314_K	1.183	1.00
169_L	173_Y	1.180	1.00
70_L	106_A	1.178	1.00
27_L	30_E	1.173	1.00
79_I	106_A	1.172	1.00
169_L	177_A	1.154	1.00
63_N	99_I	1.140	1.00
79_I	107_Y	1.138	1.00
248_Q	251_E	1.130	1.00
66_G	78_A	1.130	1.00
29_Q	44_L	1.129	1.00
115_N	118_N	1.127	1.00
108_F	120_L	1.120	1.00
178_K	182_D	1.091	1.00
231_E	234_P	1.089	1.00
121_K	126_N	1.086	1.00
59_P	85_L	1.074	1.00
97_N	123_L	1.062	1.00
300_S	304_N	1.059	1.00
37_K	68_I	1.048	1.00
139_L	158_L	1.044	1.00
270_Y	286_A	1.040	1.00
205_I	209_S	1.032	1.00
180_Y	188_K	1.028	1.00
45_D	49_S	1.020	1.00
43_F	46_R	1.014	1.00
30_E	34_L	1.010	1.00
47_A	52_N	0.994	1.00
64_I	67_L	0.989	1.00
159_L	163_N	0.981	1.00
266_L	270_Y	0.973	1.00
138_L	141_K	0.968	1.00
154_E	158_L	0.964	1.00
62_L	100_L	0.955	1.00
296_A	303_A	0.952	1.00
140_V	158_L	0.952	1.00
270_Y	282_A	0.948	1.00
144_L	159_L	0.945	1.00
283_I	306_E	0.944	1.00
231_E	235_L	0.942	1.00
44_L	48_L	0.941	1.00
174_A	177_A	0.939	1.00
141_K	145_A	0.936	1.00
67_L	82_F	0.934	1.00
63_N	66_G	0.930	1.00
46_R	50_I	0.924	1.00
86_L	100_L	0.923	1.00
124_V	136_K	0.919	1.00
108_F	145_A	0.917	1.00
68_I	72_K	0.916	1.00
51_A	58_N	0.913	1.00
62_L	136_K	0.912	1.00
281_E	284_K	0.908	1.00
113_L	142_V	0.908	1.00
105_L	108_F	0.907	1.00
263_L	267_Y	0.906	1.00
27_L	43_F	0.904	1.00
52_N	58_N	0.901	1.00
136_K	161_E	0.901	1.00
154_E	157_E	0.901	1.00
46_R	49_S	0.894	1.00
294_F	298_A	0.891	1.00
45_D	77_I	0.891	1.00
159_L	175_A	0.889	1.00
38_Y	72_K	0.887	1.00
156_R	180_Y	0.887	1.00
161_E	164_I	0.883	1.00
47_A	61_V	0.880	1.00
100_L	122_G	0.878	1.00
190_K	209_S	0.877	1.00
37_K	41_T	0.874	1.00
170_A	177_A	0.874	1.00
105_L	109_Q	0.870	1.00
300_S	303_A	0.866	1.00
262_K	266_L	0.866	1.00
250_L	254_G	0.862	1.00
296_A	300_S	0.862	1.00
48_L	61_V	0.854	1.00
297_R	304_N	0.849	0.99
59_P	63_N	0.848	0.99
133_V	162_F	0.846	0.99
162_F	172_A	0.844	0.99
177_A	180_Y	0.842	0.99
100_L	123_L	0.837	0.99
186_L	189_A	0.833	0.99
101_Y	132_N	0.832	0.99
230_S	235_L	0.830	0.99
84_E	87_S	0.821	0.99
304_N	308_E	0.818	0.99
283_I	286_A	0.814	0.99
141_K	159_L	0.812	0.99
244_S	249_I	0.809	0.99
284_K	287_E	0.808	0.99
77_I	81_T	0.805	0.99
102_N	105_L	0.805	0.99
76_D	110_K	0.805	0.99
252_K	255_N	0.803	0.99
299_Y	307_E	0.799	0.99
61_V	64_I	0.796	0.99
193_A	197_G	0.795	0.99
66_G	70_L	0.792	0.99
142_V	146_L	0.792	0.99
39_K	77_I	0.791	0.99
106_A	110_K	0.786	0.99
108_F	113_L	0.785	0.99
192_I	195_V	0.785	0.99
204_D	207_S	0.781	0.99
292_Y	295_L	0.781	0.99
159_L	172_A	0.780	0.99
117_Y	142_V	0.780	0.99
244_S	257_E	0.779	0.99
253_I	269_I	0.776	0.99
287_E	295_L	0.774	0.99
120_L	135_S	0.770	0.99
63_N	82_F	0.769	0.99
86_L	103_L	0.768	0.99
295_L	299_Y	0.765	0.99
255_N	259_S	0.764	0.99
301_E	305_Y	0.764	0.99
70_L	78_A	0.762	0.99
232_L	236_Y	0.761	0.99
205_I	208_I	0.760	0.99
118_N	122_G	0.755	0.99
38_Y	42_E	0.751	0.99
273_T	278_F	0.751	0.99
82_F	103_L	0.749	0.99
70_L	75_P	0.749	0.99
97_N	101_Y	0.749	0.99
274_K	305_Y	0.748	0.99
42_E	53_K	0.748	0.99
32_H	44_L	0.745	0.99
137_K	140_V	0.742	0.99
28_I	42_E	0.741	0.99
141_K	144_L	0.740	0.99
266_L	269_I	0.737	0.99
104_S	116_A	0.736	0.99
178_K	181_N	0.732	0.99
80_L	83_K	0.731	0.98
85_L	99_I	0.728	0.98
267_Y	301_E	0.725	0.98
203_A	208_I	0.724	0.98
158_L	161_E	0.722	0.98
267_Y	270_Y	0.720	0.98
230_S	239_S	0.711	0.98
231_E	239_S	0.710	0.98
180_Y	192_I	0.710	0.98
119_T	122_G	0.702	0.98
247_A	250_L	0.701	0.98
124_V	135_S	0.699	0.98
75_P	79_I	0.698	0.98
215_L	219_I	0.692	0.98
234_P	237_K	0.692	0.98
206_L	210_D	0.691	0.98
307_E	312_L	0.691	0.98
158_L	221_I	0.688	0.98
78_A	85_L	0.688	0.98
104_S	108_F	0.688	0.98
289_L	294_F	0.687	0.98
303_A	307_E	0.685	0.98
279_L	309_V	0.683	0.98
230_S	234_P	0.683	0.98
173_Y	195_V	0.682	0.98
269_I	286_A	0.680	0.98
58_N	96_K	0.677	0.98
29_Q	64_I	0.675	0.97
129_G	132_N	0.675	0.97
60_T	64_I	0.673	0.97
75_P	106_A	0.673	0.97
288_K	291_D	0.671	0.97
43_F	47_A	0.666	0.97
99_I	102_N	0.664	0.97
254_G	258_D	0.660	0.97
221_I	224_K	0.659	0.97
266_L	289_L	0.659	0.97
294_F	307_E	0.659	0.97
268_D	271_R	0.659	0.97
96_K	99_I	0.658	0.97
42_E	46_R	0.658	0.97
101_Y	123_L	0.654	0.97
209_S	214_D	0.653	0.97
48_L	62_L	0.652	0.97
47_A	51_A	0.651	0.97
181_N	193_A	0.650	0.97
49_S	52_N	0.650	0.97
62_L	69_Y	0.642	0.97
262_K	272_R	0.642	0.97
101_Y	104_S	0.640	0.97
67_L	71_E	0.638	0.96
32_H	37_K	0.636	0.96
79_I	103_L	0.634	0.96
203_A	234_P	0.631	0.96
105_L	138_L	0.629	0.96
160_E	164_I	0.628	0.96
36_K	74_R	0.628	0.96
297_R	301_E	0.627	0.96
233_K	236_Y	0.627	0.96
230_S	236_Y	0.624	0.96
60_T	63_N	0.623	0.96
137_K	172_A	0.622	0.96
298_A	306_E	0.619	0.96
62_L	84_E	0.619	0.96
123_L	127_S	0.616	0.96
115_N	119_T	0.614	0.96
173_Y	177_A	0.614	0.96
28_I	40_N	0.614	0.96
120_L	142_V	0.612	0.96
234_P	241_K	0.611	0.96
205_I	210_D	0.608	0.95
292_Y	296_A	0.607	0.95
204_D	208_I	0.606	0.95
58_N	62_L	0.605	0.95
31_Y	69_Y	0.604	0.95
218_A	225_L	0.602	0.95
304_N	307_E	0.601	0.95
299_Y	305_Y	0.598	0.95
221_I	225_L	0.598	0.95
123_L	135_S	0.597	0.95
108_F	138_L	0.596	0.95
144_L	175_A	0.596	0.95
43_F	119_T	0.596	0.95
288_K	292_Y	0.595	0.95
286_A	290_Q	0.595	0.95
80_L	84_E	0.592	0.95
236_Y	265_I	0.590	0.95
246_F	250_L	0.589	0.95
31_Y	34_L	0.588	0.94
162_F	167_E	0.588	0.94
66_G	102_N	0.588	0.94
169_L	174_A	0.585	0.94
116_A	142_V	0.584	0.94
105_L	120_L	0.582	0.94
296_A	301_E	0.579	0.94
298_A	308_E	0.579	0.94
180_Y	184_K	0.578	0.94
177_A	189_A	0.578	0.94
234_P	239_S	0.577	0.94
30_E	48_L	0.576	0.94
119_T	123_L	0.575	0.94
37_K	71_E	0.574	0.94
39_K	115_N	0.573	0.94
279_L	305_Y	0.569	0.93
228_H	231_E	0.568	0.93
294_F	300_S	0.567	0.93
79_I	112_E	0.564	0.93
305_Y	311_N	0.563	0.93
70_L	73_R	0.562	0.93
95_D	99_I	0.562	0.93
153_E	157_E	0.561	0.93
245_L	249_I	0.561	0.93
231_E	241_K	0.559	0.93
37_K	72_K	0.559	0.93
209_S	217_K	0.558	0.93
63_N	67_L	0.558	0.93
298_A	301_E	0.558	0.93
160_E	176_L	0.555	0.93
249_I	252_K	0.554	0.93
39_K	43_F	0.552	0.92
132_N	135_S	0.552	0.92
97_N	132_N	0.551	0.92
82_F	86_L	0.551	0.92
177_A	184_K	0.550	0.92
34_L	43_F	0.549	0.92
123_L	129_G	0.549	0.92
40_N	44_L	0.548	0.92
249_I	257_E	0.548	0.92
34_L	37_K	0.545	0.92
122_G	127_S	0.545	0.92
273_T	276_L	0.544	0.92
134_L	137_K	0.544	0.92
270_Y	273_T	0.541	0.92
84_E	88_N	0.539	0.92
184_K	189_A	0.538	0.91
222_Y	226_G	0.536	0.91
231_E	237_K	0.536	0.91
137_K	175_A	0.535	0.91
144_L	152_I	0.535	0.91
120_L	124_V	0.535	0.91
301_E	308_E	0.534	0.91
235_L	238_L	0.533	0.91
270_Y	289_L	0.533	0.91
29_Q	33_N	0.533	0.91
83_K	87_S	0.531	0.91
191_D	195_V	0.531	0.91
282_A	285_T	0.531	0.91
62_L	66_G	0.531	0.91
309_V	313_R	0.530	0.91
28_I	32_H	0.530	0.91
290_Q	293_L	0.529	0.91
48_L	52_N	0.528	0.91
250_L	253_I	0.528	0.91
290_Q	295_L	0.527	0.91
291_D	294_F	0.526	0.91
298_A	302_L	0.526	0.91
202_L	210_D	0.526	0.91
297_R	312_L	0.526	0.91
166_S	169_L	0.525	0.90
229_N	237_K	0.524	0.90
262_K	267_Y	0.523	0.90
78_A	82_F	0.522	0.90
171_I	175_A	0.518	0.90
66_G	81_T	0.518	0.90
269_I	272_R	0.518	0.90
299_Y	306_E	0.518	0.90
308_E	312_L	0.517	0.90
143_C	146_L	0.516	0.90
97_N	133_V	0.515	0.90
136_K	143_C	0.514	0.90
58_N	61_V	0.514	0.90
290_Q	294_F	0.514	0.90
177_A	182_D	0.513	0.89
286_A	294_F	0.512	0.89
136_K	162_F	0.512	0.89
286_A	296_A	0.510	0.89
305_Y	309_V	0.509	0.89
233_K	237_K	0.509	0.89
64_I	68_I	0.508	0.89
235_L	240_N	0.506	0.89
301_E	306_E	0.505	0.89
37_K	42_E	0.503	0.89
299_Y	309_V	0.501	0.88
163_N	176_L	0.501	0.88
176_L	179_Y	0.500	0.88

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1w3bA	2	0.9604	100	0.278	Contact Map
5aioA	1	0.8902	100	0.293	Contact Map
4kvmA	3	0.9512	100	0.296	Contact Map
4ui9X	2	0.9726	100	0.297	Contact Map
4bujB	1	0.9634	100	0.301	Contact Map
4ui9F	2	0.8872	100	0.302	Contact Map
2y4tA	1	0.9055	100	0.314	Contact Map
2xpiA	2	0.9573	100	0.317	Contact Map
4ui9C	2	0.9634	100	0.322	Contact Map
4rg9A	2	0.8232	100	0.322	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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