Q97ZK8 - SULSO - GREMLIN Contacts

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Q97ZK8 - Uncharacterized protein

UniProt:	Q97ZK8	Sequences: MRIAILGGGVAGSTLAYLLSRINYEVTIFDINQHYVKPCGDIVPNIYTPPFNWKETFGIRRFSFYIDGEKIYDVEYRHTKWLVIDKWKWINDMRKSSKVIVTHDNNAKEFDYVIDSRGPYPMDREVVYTTRAIIETEEFDDEAILEFNTRYTGFYWIFPSKENEYNIGAGFLEYKNSKELLNYYLRKRFKNFEVKDIRGAPISIGSVKNKKLRIGEARGLVFPLSGEGIRPSAISAEYAFEAIHKEKDLDGFLNEKLSIIEKRIKIQKMLLDLYRYSSLNLRKTFMRIFFKNEVLIDTYLEDKMDLNGIIESLKSVRANGGIIRKFEEQER	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	331 (277)
Sequences:	25485 (19349)
Seq/√Len:	1162.5

ECOLI - YDIS UBIH MHPA UBIF UBII YGCN FIXC CBRA
BACSU - YETM YHJG
HALSA - Q9HS24 Q9HQE5 Q9HMA0 Q9HS01
SULSO - Q97ZK8 Q97V18 Q97W89 Q97V59

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
4_A	110_F	4.251	1.00
11_A	232_S	4.019	1.00
257_L	261_E	3.446	1.00
2_R	25_E	3.203	1.00
22_I	244_H	3.161	1.00
29_F	108_K	3.067	1.00
2_R	111_D	3.067	1.00
18_L	240_F	2.906	1.00
15_L	114_I	2.880	1.00
4_A	29_F	2.850	1.00
178_K	182_N	2.770	1.00
17_Y	96_S	2.591	1.00
11_A	214_I	2.527	1.00
115_D	123_D	2.459	1.00
242_A	259_I	2.374	1.00
91_N	95_K	2.331	1.00
242_A	255_E	2.230	1.00
14_T	233_A	2.199	1.00
100_I	107_A	2.183	1.00
50_P	237_E	2.051	1.00
261_E	265_K	2.047	1.00
47_Y	230_R	2.043	1.00
24_Y	244_H	2.042	1.00
236_A	240_F	2.015	1.00
115_D	213_R	2.011	1.00
182_N	186_R	1.999	1.00
100_I	109_E	1.978	1.00
116_S	214_I	1.863	1.00
98_K	109_E	1.853	1.00
238_Y	259_I	1.793	1.00
16_A	28_I	1.786	1.00
184_Y	188_R	1.784	1.00
17_Y	21_R	1.768	1.00
238_Y	242_A	1.751	1.00
122_M	126_V	1.749	1.00
27_T	108_K	1.737	1.00
248_D	251_G	1.737	1.00
3_I	19_L	1.734	1.00
15_L	236_A	1.715	1.00
53_W	237_E	1.712	1.00
102_T	107_A	1.702	1.00
2_R	27_T	1.691	1.00
127_V	211_K	1.688	1.00
3_I	24_Y	1.684	1.00
117_R	121_P	1.635	1.00
114_I	214_I	1.622	1.00
180_L	183_Y	1.611	1.00
112_Y	253_L	1.609	1.00
29_F	103_H	1.589	1.00
103_H	108_K	1.584	1.00
179_E	182_N	1.582	1.00
235_S	260_I	1.580	1.00
179_E	183_Y	1.566	1.00
197_I	200_A	1.553	1.00
212_L	239_A	1.553	1.00
185_L	188_R	1.552	1.00
234_I	263_R	1.526	1.00
10_V	229_I	1.524	1.00
4_A	108_K	1.514	1.00
183_Y	186_R	1.513	1.00
181_L	185_L	1.513	1.00
4_A	101_V	1.505	1.00
103_H	106_N	1.503	1.00
242_A	252_F	1.474	1.00
194_V	197_I	1.452	1.00
180_L	184_Y	1.445	1.00
183_Y	187_K	1.396	1.00
242_A	245_K	1.385	1.00
102_T	105_N	1.383	1.00
114_I	212_L	1.381	1.00
241_E	259_I	1.362	1.00
119_P	220_L	1.361	1.00
17_Y	97_S	1.340	1.00
132_A	135_E	1.332	1.00
16_A	26_V	1.316	1.00
15_L	19_L	1.314	1.00
219_G	235_S	1.310	1.00
122_M	125_E	1.291	1.00
129_T	133_I	1.289	1.00
47_Y	233_A	1.288	1.00
128_Y	131_R	1.287	1.00
200_A	205_G	1.283	1.00
70_K	73_D	1.283	1.00
8_G	13_S	1.277	1.00
69_E	72_Y	1.273	1.00
5_I	15_L	1.273	1.00
88_K	91_N	1.262	1.00
4_A	27_T	1.246	1.00
85_D	88_K	1.245	1.00
6_L	123_D	1.229	1.00
200_A	203_S	1.223	1.00
130_T	133_I	1.221	1.00
181_L	184_Y	1.207	1.00
135_E	138_E	1.200	1.00
133_I	136_T	1.189	1.00
13_S	97_S	1.182	1.00
59_I	62_F	1.180	1.00
178_K	181_L	1.179	1.00
207_V	212_L	1.170	1.00
130_T	134_I	1.159	1.00
129_T	137_E	1.159	1.00
14_T	18_L	1.156	1.00
221_V	268_K	1.152	1.00
189_F	194_V	1.152	1.00
197_I	201_P	1.147	1.00
6_L	117_R	1.137	1.00
254_N	258_S	1.134	1.00
27_T	110_F	1.131	1.00
110_F	113_V	1.129	1.00
156_W	168_G	1.125	1.00
20_S	26_V	1.122	1.00
265_K	269_M	1.118	1.00
5_I	16_A	1.118	1.00
221_V	231_P	1.106	1.00
238_Y	241_E	1.102	1.00
6_L	113_V	1.097	1.00
15_L	214_I	1.096	1.00
200_A	204_I	1.096	1.00
41_D	229_I	1.094	1.00
214_I	256_K	1.088	1.00
18_L	22_I	1.087	1.00
98_K	103_H	1.074	1.00
213_R	217_A	1.073	1.00
92_D	95_K	1.070	1.00
166_N	170_G	1.061	1.00
178_K	185_L	1.052	1.00
92_D	96_S	1.050	1.00
201_P	205_G	1.038	1.00
53_W	241_E	1.038	1.00
20_S	97_S	1.035	1.00
178_K	188_R	1.034	1.00
23_N	26_V	1.034	1.00
181_L	188_R	1.031	1.00
21_R	96_S	1.031	1.00
245_K	255_E	1.023	1.00
198_R	201_P	1.020	1.00
207_V	257_L	1.020	1.00
255_E	258_S	1.017	1.00
128_Y	132_A	1.010	1.00
86_K	90_I	1.010	1.00
250_D	254_N	1.007	1.00
131_R	135_E	1.006	1.00
59_I	64_F	1.003	1.00
5_I	26_V	0.999	1.00
10_V	233_A	0.994	1.00
2_R	24_Y	0.989	1.00
199_G	204_I	0.985	1.00
182_N	185_L	0.983	1.00
19_L	24_Y	0.981	1.00
197_I	205_G	0.970	1.00
41_D	86_K	0.969	1.00
18_L	21_R	0.967	1.00
212_L	256_K	0.959	1.00
136_T	139_F	0.958	1.00
199_G	203_S	0.954	1.00
129_T	132_A	0.952	1.00
225_S	271_L	0.948	1.00
90_I	94_R	0.942	1.00
130_T	135_E	0.938	1.00
137_E	140_D	0.938	1.00
61_R	144_I	0.935	1.00
129_T	136_T	0.926	1.00
130_T	136_T	0.921	1.00
38_P	41_D	0.919	1.00
99_V	103_H	0.918	1.00
131_R	139_F	0.913	1.00
208_K	254_N	0.910	1.00
237_E	241_E	0.905	1.00
198_R	203_S	0.905	1.00
202_I	205_G	0.904	1.00
121_P	124_R	0.903	1.00
128_Y	133_I	0.903	1.00
19_L	240_F	0.903	1.00
99_V	210_K	0.902	1.00
181_L	186_R	0.898	1.00
66_I	70_K	0.895	1.00
249_L	253_L	0.894	1.00
134_I	137_E	0.891	1.00
115_D	211_K	0.890	1.00
46_I	227_E	0.887	1.00
48_T	82_L	0.885	1.00
192_F	195_K	0.884	1.00
13_S	90_I	0.878	1.00
49_P	52_N	0.874	1.00
18_L	237_E	0.871	1.00
129_T	135_E	0.871	1.00
13_S	94_R	0.871	1.00
221_V	225_S	0.870	1.00
167_I	170_G	0.869	1.00
262_K	266_I	0.867	1.00
26_V	97_S	0.866	1.00
46_I	49_P	0.865	1.00
16_A	97_S	0.863	1.00
184_Y	187_K	0.862	1.00
115_D	121_P	0.859	1.00
29_F	110_F	0.857	1.00
255_E	259_I	0.854	1.00
112_Y	212_L	0.848	1.00
201_P	204_I	0.846	1.00
123_D	211_K	0.842	1.00
240_F	244_H	0.840	1.00
272_D	276_Y	0.832	1.00
213_R	218_R	0.830	1.00
42_I	81_W	0.830	1.00
191_N	194_V	0.830	1.00
46_I	234_I	0.829	1.00
29_F	101_V	0.827	1.00
204_I	272_D	0.826	1.00
47_Y	50_P	0.823	1.00
19_L	243_I	0.821	1.00
209_N	250_D	0.817	1.00
22_I	240_F	0.817	1.00
199_G	202_I	0.815	1.00
99_V	113_V	0.815	1.00
249_L	252_F	0.808	1.00
136_T	140_D	0.807	1.00
166_N	171_F	0.806	1.00
8_G	30_D	0.806	1.00
143_A	146_E	0.805	1.00
14_T	93_M	0.805	1.00
259_I	262_K	0.804	1.00
48_T	52_N	0.804	1.00
63_S	73_D	0.803	1.00
61_R	73_D	0.802	1.00
51_F	233_A	0.797	1.00
220_L	235_S	0.795	1.00
150_R	153_G	0.794	1.00
114_I	239_A	0.793	1.00
208_K	212_L	0.793	1.00
129_T	134_I	0.792	1.00
248_D	252_F	0.792	1.00
167_I	171_F	0.787	1.00
143_A	148_N	0.782	1.00
229_I	232_S	0.778	1.00
63_S	66_I	0.773	1.00
2_R	108_K	0.772	1.00
203_S	206_S	0.769	1.00
131_R	134_I	0.764	1.00
132_A	136_T	0.758	1.00
198_R	202_I	0.757	1.00
85_D	161_K	0.754	1.00
32_N	35_Y	0.752	1.00
178_K	183_Y	0.752	1.00
88_K	161_K	0.752	1.00
17_Y	20_S	0.750	1.00
67_D	70_K	0.744	1.00
11_A	114_I	0.744	1.00
178_K	187_K	0.741	1.00
60_R	143_A	0.739	1.00
145_L	148_N	0.736	1.00
200_A	206_S	0.734	1.00
229_I	233_A	0.733	1.00
8_G	28_I	0.732	1.00
251_G	255_E	0.730	1.00
50_P	53_W	0.728	1.00
198_R	204_I	0.728	1.00
130_T	138_E	0.727	1.00
63_S	71_I	0.726	1.00
115_D	127_V	0.726	1.00
131_R	136_T	0.721	1.00
197_I	203_S	0.711	1.00
182_N	188_R	0.710	1.00
101_V	106_N	0.709	1.00
101_V	113_V	0.706	1.00
246_E	249_L	0.702	1.00
231_P	267_Q	0.702	1.00
174_Y	177_S	0.700	1.00
59_I	65_Y	0.699	1.00
190_K	196_D	0.698	1.00
84_I	89_W	0.698	1.00
196_D	199_G	0.697	1.00
191_N	195_K	0.696	1.00
60_R	65_Y	0.695	1.00
134_I	138_E	0.693	1.00
68_G	71_I	0.690	1.00
227_E	231_P	0.687	1.00
128_Y	135_E	0.686	1.00
197_I	202_I	0.680	1.00
42_I	83_V	0.678	1.00
202_I	206_S	0.676	1.00
2_R	110_F	0.675	1.00
189_F	192_F	0.672	1.00
112_Y	243_I	0.671	1.00
130_T	137_E	0.670	1.00
264_I	268_K	0.670	1.00
231_P	264_I	0.669	1.00
196_D	200_A	0.668	1.00
63_S	144_I	0.667	1.00
54_K	57_F	0.662	1.00
44_P	230_R	0.661	1.00
207_V	253_L	0.661	1.00
62_F	66_I	0.658	1.00
224_L	275_R	0.656	1.00
65_Y	146_E	0.654	1.00
68_G	72_Y	0.654	1.00
251_G	254_N	0.652	1.00
131_R	138_E	0.652	1.00
112_Y	249_L	0.652	1.00
205_G	220_L	0.646	1.00
113_V	211_K	0.644	1.00
269_M	273_L	0.644	1.00
15_L	240_F	0.644	1.00
225_S	274_Y	0.643	1.00
66_I	69_E	0.642	1.00
123_D	126_V	0.639	1.00
18_L	50_P	0.632	1.00
63_S	70_K	0.630	1.00
208_K	211_K	0.629	1.00
135_E	140_D	0.628	1.00
135_E	139_F	0.625	1.00
13_S	28_I	0.624	1.00
214_I	236_A	0.623	1.00
189_F	195_K	0.622	1.00
52_N	79_T	0.621	1.00
212_L	253_L	0.620	1.00
132_A	139_F	0.620	1.00
144_I	147_F	0.620	1.00
178_K	186_R	0.620	1.00
241_E	245_K	0.619	1.00
74_V	77_R	0.619	1.00
158_F	168_G	0.613	1.00
165_Y	169_A	0.613	1.00
144_I	148_N	0.611	1.00
24_Y	243_I	0.609	1.00
128_Y	136_T	0.609	1.00
266_I	269_M	0.607	1.00
180_L	187_K	0.606	1.00
124_R	213_R	0.605	1.00
142_E	146_E	0.605	1.00
221_V	271_L	0.604	1.00
156_W	160_S	0.603	1.00
69_E	73_D	0.602	1.00
182_N	187_K	0.601	1.00
193_E	196_D	0.599	1.00
46_I	263_R	0.597	1.00
115_D	122_M	0.597	1.00
132_A	137_E	0.595	1.00
219_G	260_I	0.595	1.00
133_I	138_E	0.590	1.00
234_I	238_Y	0.589	1.00
180_L	188_R	0.589	1.00
39_C	86_K	0.589	1.00
135_E	164_E	0.589	1.00
160_S	164_E	0.583	1.00
51_F	89_W	0.582	1.00
254_N	257_L	0.580	1.00
50_P	234_I	0.580	1.00
208_K	250_D	0.580	1.00
62_F	65_Y	0.579	1.00
195_K	198_R	0.578	1.00
131_R	137_E	0.578	1.00
161_K	164_E	0.577	1.00
141_D	144_I	0.577	1.00
113_V	210_K	0.575	1.00
87_W	91_N	0.575	1.00
65_Y	71_I	0.571	1.00
18_L	236_A	0.570	1.00
234_I	260_I	0.570	1.00
258_S	262_K	0.569	1.00
6_L	31_I	0.568	1.00
133_I	140_D	0.568	1.00
178_K	184_Y	0.566	1.00
219_G	264_I	0.566	1.00
167_I	172_L	0.565	1.00
196_D	205_G	0.564	1.00
130_T	139_F	0.562	1.00
134_I	140_D	0.559	1.00
227_E	230_R	0.559	1.00
201_P	206_S	0.558	1.00
49_P	53_W	0.558	1.00
128_Y	137_E	0.554	1.00
43_V	47_Y	0.554	1.00
179_E	186_R	0.552	1.00
68_G	73_D	0.551	1.00
67_D	73_D	0.550	1.00
45_N	49_P	0.548	1.00
166_N	169_A	0.547	1.00
190_K	195_K	0.546	1.00
164_E	167_I	0.545	1.00
58_G	62_F	0.545	1.00
4_A	99_V	0.543	1.00
210_K	246_E	0.542	1.00
227_E	267_Q	0.541	1.00
133_I	137_E	0.541	1.00
31_I	120_Y	0.540	1.00
238_Y	256_K	0.539	1.00
171_F	175_K	0.538	1.00
115_D	217_A	0.535	1.00
270_L	273_L	0.534	1.00
128_Y	134_I	0.532	1.00
101_V	110_F	0.531	1.00
52_N	78_H	0.530	1.00
111_D	210_K	0.529	1.00
199_G	205_G	0.529	1.00
67_D	72_Y	0.529	1.00
22_I	241_E	0.527	1.00
25_E	111_D	0.527	1.00
135_E	193_E	0.526	1.00
190_K	194_V	0.522	1.00
197_I	204_I	0.521	1.00
101_V	108_K	0.520	1.00
115_D	126_V	0.517	1.00
192_F	196_D	0.517	1.00
66_I	71_I	0.517	1.00
132_A	140_D	0.517	1.00
88_K	92_D	0.515	1.00
112_Y	250_D	0.514	1.00
58_G	80_K	0.513	1.00
12_G	116_S	0.511	1.00
274_Y	277_S	0.509	1.00
222_F	225_S	0.506	1.00
182_N	190_K	0.506	1.00
212_L	257_L	0.505	1.00
259_I	263_R	0.503	1.00
11_A	236_A	0.502	1.00
199_G	206_S	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1pn0A	3	1	100	0.424	Contact Map
2dkhA	2	0.9637	100	0.442	Contact Map
3aljA	1	0.9245	100	0.442	Contact Map
2qa1A	2	0.9637	100	0.444	Contact Map
3rp8A	1	0.9426	100	0.446	Contact Map
4bjzA	2	0.9456	100	0.446	Contact Map
2qa2A	2	0.9668	100	0.447	Contact Map
2x3nA	1	0.8671	100	0.448	Contact Map
4k22A	4	0.8429	100	0.448	Contact Map
4j33A	1	0.8701	100	0.45	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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