GATD - SULSO - GREMLIN Contacts

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GATD - Glutamyl-tRNA(Gln) amidotransferase subunit D

UniProt:	Q97ZH5	Sequences: MQENYKGKAYDILKNLNIEEGDLIEIKKGDLRIRGILLPSYSKDDRIFVIKLDNGYNIGISIDNITEIKPIEKKSSKDRESERREVHNGAKSEIKIISTGGTIVSRVEYETGAVRPALTTEEIIQFLPEINEIAKVDAEVLFSILSENMKPEFWVKIAESVKKAFDEGNTGIVIAHGTDTMAYTASALAFSLRSLQGPVVLVGSQRSSDRPSSDSAINLLSAVITAKYAPFGEVVVNMHAESSDTYALVHRGVKVRKMHSSRRDAFQSVNDKPLAKVLWKERKLVMLNEKYISKKDETLLDAKFDNRVFLLYYYPGLDRDFLEQMLTNTKIRGIIIAGTGLGHTSSDHIELFRKATKDGIFIGMTTQCLFGRVNMNVYTTGRQLLDAGVTPLEDMLPEVALVKLMWVLAHEQDLEKIQNLMITNLVGEINPRHTLDLFPRWSYE	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	444 (339)
Sequences:	682 (475)
Seq/√Len:	25.8

ECOLI - ASPG1 ASPG2
BACSU - ASPG2 ASPG1
HALSA - GATD
SULSO - GATD

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
312_Y	337_A	7.102	1.00
184_T	201_L	6.321	1.00
192_L	199_V	6.169	1.00
188_L	201_L	4.204	1.00
413_D	416_K	4.015	1.00
98_S	104_V	3.971	1.00
233_E	274_L	3.678	1.00
236_V	255_V	3.672	1.00
353_R	357_K	3.664	1.00
233_E	251_R	3.663	1.00
260_S	366_T	3.529	1.00
250_H	255_V	3.486	1.00
113_A	116_P	3.248	1.00
363_G	392_L	3.208	1.00
323_E	354_K	3.171	1.00
141_L	160_S	3.135	1.00
305_D	332_R	2.979	1.00
161_V	188_L	2.961	1.00
363_G	421_M	2.786	1.00
407_V	420_L	2.764	0.99
189_A	253_V	2.719	0.99
97_I	141_L	2.649	0.99
165_F	197_G	2.581	0.99
173_V	226_A	2.477	0.99
361_F	390_T	2.422	0.99
93_E	135_K	2.406	0.99
414_L	418_Q	2.306	0.98
364_M	389_V	2.298	0.98
204_S	260_S	2.268	0.98
338_G	364_M	2.267	0.98
337_A	397_P	2.243	0.98
407_V	411_E	2.236	0.98
326_L	360_I	2.192	0.97
249_V	275_A	2.191	0.97
323_E	351_L	2.156	0.97
416_K	419_N	2.076	0.96
333_G	400_A	2.065	0.96
94_I	291_Y	2.041	0.96
326_L	355_A	2.041	0.96
237_N	249_V	2.032	0.96
225_T	237_N	2.025	0.96
363_G	404_L	2.021	0.96
333_G	404_L	1.979	0.95
103_I	215_S	1.918	0.94
311_L	322_L	1.890	0.94
310_L	397_P	1.822	0.92
159_E	302_A	1.801	0.92
361_F	417_I	1.787	0.92
270_N	394_D	1.751	0.91
361_F	414_L	1.710	0.90
173_V	223_V	1.698	0.89
231_F	284_L	1.682	0.89
162_K	300_L	1.656	0.88
144_I	149_M	1.646	0.88
144_I	153_F	1.645	0.88
182_A	398_E	1.595	0.86
201_L	252_G	1.588	0.86
94_I	418_Q	1.562	0.85
237_N	277_V	1.560	0.85
155_V	191_S	1.534	0.84
114_V	207_S	1.531	0.84
196_Q	298_T	1.463	0.81
193_R	301_D	1.443	0.80
200_V	235_V	1.438	0.80
158_A	300_L	1.436	0.80
123_I	140_V	1.435	0.79
201_L	274_L	1.410	0.78
225_T	249_V	1.403	0.78
406_W	410_H	1.398	0.78
254_K	315_P	1.394	0.77
137_D	168_G	1.393	0.77
185_A	400_A	1.390	0.77
165_F	172_I	1.385	0.77
195_L	199_V	1.384	0.77
254_K	430_N	1.379	0.77
97_I	226_A	1.378	0.77
206_R	212_S	1.366	0.76
335_I	363_G	1.360	0.76
365_T	397_P	1.353	0.75
399_V	402_V	1.350	0.75
412_Q	416_K	1.348	0.75
251_R	274_L	1.330	0.74
168_G	197_G	1.320	0.73
130_I	141_L	1.308	0.73
417_I	421_M	1.301	0.72
109_Y	114_V	1.294	0.72
359_G	418_Q	1.280	0.71
151_P	316_G	1.268	0.70
213_S	216_A	1.267	0.70
176_H	184_T	1.256	0.70
152_E	306_N	1.229	0.68
366_T	372_R	1.219	0.67
364_M	384_L	1.217	0.67
206_R	263_R	1.216	0.67
265_A	405_M	1.211	0.67
161_V	174_I	1.206	0.66
185_A	255_V	1.197	0.66
130_I	171_G	1.194	0.66
313_Y	345_S	1.192	0.65
257_K	340_G	1.190	0.65
393_E	429_I	1.179	0.65
114_V	117_A	1.178	0.64
144_I	339_T	1.177	0.64
159_E	300_L	1.176	0.64
181_M	236_V	1.176	0.64
193_R	299_L	1.170	0.64
152_E	419_N	1.165	0.64
163_K	386_D	1.153	0.63
256_R	259_H	1.153	0.63
407_V	417_I	1.145	0.62
348_H	357_K	1.137	0.62
106_R	116_P	1.135	0.62
160_S	163_K	1.121	0.61
240_A	248_L	1.121	0.61
382_R	385_L	1.119	0.60
378_Y	381_G	1.112	0.60
322_L	352_F	1.111	0.60
376_N	381_G	1.111	0.60
103_I	204_S	1.110	0.60
191_S	304_F	1.103	0.59
165_F	168_G	1.101	0.59
92_S	134_A	1.095	0.59
221_S	237_N	1.081	0.58
139_E	160_S	1.077	0.57
385_L	406_W	1.062	0.56
164_A	413_D	1.062	0.56
194_S	297_E	1.055	0.56
384_L	389_V	1.053	0.56
130_I	220_L	1.052	0.56
325_M	330_K	1.049	0.55
375_M	381_G	1.045	0.55
343_H	364_M	1.044	0.55
112_G	174_I	1.043	0.55
209_D	259_H	1.042	0.55
110_E	116_P	1.040	0.55
215_S	309_F	1.021	0.53
200_V	249_V	1.019	0.53
236_V	306_N	1.018	0.53
235_V	275_A	1.018	0.53
95_K	164_A	1.006	0.52
256_R	306_N	1.004	0.52
335_I	392_L	1.000	0.52
94_I	227_K	0.996	0.52
154_W	315_P	0.993	0.51
250_H	268_S	0.990	0.51
150_K	222_A	0.985	0.51
339_T	343_H	0.985	0.51
94_I	170_T	0.978	0.50
186_S	402_V	0.977	0.50
150_K	304_F	0.974	0.50
106_R	119_T	0.973	0.50
149_M	184_T	0.971	0.50
127_L	130_I	0.970	0.50
121_E	222_A	0.970	0.50
213_S	274_L	0.968	0.49
239_H	366_T	0.968	0.49
297_E	416_K	0.967	0.49
148_N	380_T	0.966	0.49
150_K	153_F	0.966	0.49
108_E	417_I	0.953	0.48
332_R	409_A	0.953	0.48
95_K	139_E	0.951	0.48
233_E	249_V	0.948	0.48
147_E	343_H	0.943	0.48
162_K	166_D	0.939	0.47
133_I	136_V	0.935	0.47
322_L	348_H	0.932	0.47
239_H	264_D	0.922	0.46
192_L	252_G	0.921	0.46
289_E	413_D	0.921	0.46
259_H	370_F	0.916	0.46
364_M	375_M	0.916	0.46
161_V	172_I	0.914	0.45
124_I	418_Q	0.909	0.45
378_Y	388_G	0.904	0.45
317_L	345_S	0.901	0.45
235_V	274_L	0.900	0.44
97_I	139_E	0.894	0.44
332_R	418_Q	0.890	0.44
193_R	426_V	0.889	0.44
192_L	360_I	0.888	0.44
147_E	238_M	0.887	0.44
181_M	344_T	0.887	0.44
108_E	115_R	0.886	0.43
111_T	210_R	0.883	0.43
323_E	353_R	0.882	0.43
125_Q	222_A	0.882	0.43
133_I	202_V	0.878	0.43
268_S	394_D	0.873	0.43
101_G	177_G	0.873	0.43
100_G	266_F	0.872	0.42
96_I	141_L	0.870	0.42
361_F	407_V	0.868	0.42
404_L	421_M	0.866	0.42
230_P	360_I	0.866	0.42
159_E	162_K	0.865	0.42
334_I	355_A	0.864	0.42
254_K	394_D	0.863	0.42
212_S	338_G	0.860	0.42
183_Y	367_Q	0.857	0.41
333_G	365_T	0.855	0.41
256_R	263_R	0.853	0.41
324_Q	329_T	0.852	0.41
190_F	428_E	0.848	0.41
204_S	261_S	0.846	0.41
256_R	397_P	0.843	0.40
210_R	239_H	0.840	0.40
294_K	298_T	0.840	0.40
331_I	417_I	0.839	0.40
212_S	279_W	0.838	0.40
136_V	204_S	0.835	0.40
284_L	418_Q	0.833	0.40
170_T	197_G	0.832	0.40
236_V	250_H	0.829	0.39
165_F	194_S	0.829	0.39
181_M	222_A	0.827	0.39
188_L	252_G	0.826	0.39
411_E	416_K	0.823	0.39
171_G	198_P	0.822	0.39
209_D	213_S	0.822	0.39
168_G	172_I	0.821	0.39
331_I	390_T	0.820	0.39
178_T	340_G	0.812	0.38
386_D	424_N	0.811	0.38
356_T	387_A	0.811	0.38
184_T	187_A	0.806	0.38
157_I	164_A	0.805	0.38
351_L	354_K	0.805	0.38
235_V	249_V	0.804	0.38
355_A	360_I	0.801	0.37
154_W	388_G	0.801	0.37
94_I	139_E	0.800	0.37
338_G	343_H	0.798	0.37
200_V	302_A	0.791	0.37
311_L	333_G	0.790	0.37
154_W	394_D	0.784	0.36
186_S	253_V	0.780	0.36
168_G	171_G	0.779	0.36
154_W	402_V	0.778	0.36
129_E	216_A	0.777	0.36
145_L	339_T	0.773	0.36
174_I	201_L	0.773	0.36
98_S	148_N	0.765	0.35
202_V	237_N	0.764	0.35
200_V	220_L	0.763	0.35
150_K	395_M	0.762	0.35
128_P	173_V	0.759	0.35
205_Q	263_R	0.759	0.35
95_K	168_G	0.756	0.34
97_I	150_K	0.752	0.34
200_V	415_E	0.752	0.34
263_R	317_L	0.751	0.34
127_L	201_L	0.746	0.34
176_H	203_G	0.746	0.34
210_R	264_D	0.745	0.34
318_D	321_F	0.744	0.34
96_I	407_V	0.744	0.34
336_I	352_F	0.743	0.34
100_G	177_G	0.743	0.34
406_W	426_V	0.740	0.33
238_M	263_R	0.738	0.33
365_T	392_L	0.737	0.33
207_S	239_H	0.734	0.33
419_N	423_T	0.734	0.33
258_M	267_Q	0.727	0.32
321_F	325_M	0.726	0.32
227_K	291_Y	0.726	0.32
97_I	156_K	0.721	0.32
157_I	171_G	0.721	0.32
367_Q	398_E	0.720	0.32
345_S	416_K	0.720	0.32
367_Q	378_Y	0.720	0.32
97_I	174_I	0.719	0.32
148_N	172_I	0.718	0.32
176_H	252_G	0.717	0.32
305_D	311_L	0.715	0.32
198_P	263_R	0.714	0.32
186_S	249_V	0.713	0.32
133_I	227_K	0.712	0.32
103_I	176_H	0.712	0.32
156_K	335_I	0.711	0.31
224_I	228_Y	0.709	0.31
181_M	413_D	0.706	0.31
84_R	359_G	0.706	0.31
381_G	384_L	0.702	0.31
193_R	336_I	0.702	0.31
378_Y	384_L	0.702	0.31
194_S	291_Y	0.702	0.31
200_V	226_A	0.702	0.31
186_S	189_A	0.698	0.31
172_I	199_V	0.696	0.31
117_A	391_P	0.695	0.30
154_W	368_C	0.694	0.30
235_V	331_I	0.694	0.30
403_K	428_E	0.693	0.30
164_A	167_E	0.689	0.30
159_E	352_F	0.687	0.30
231_F	406_W	0.687	0.30
100_G	257_K	0.682	0.30
124_I	138_A	0.682	0.30
223_V	392_L	0.681	0.30
326_L	425_L	0.681	0.30
111_T	222_A	0.680	0.30
163_K	419_N	0.679	0.29
129_E	220_L	0.679	0.29
316_G	344_T	0.678	0.29
107_V	210_R	0.678	0.29
138_A	141_L	0.678	0.29
364_M	373_V	0.677	0.29
201_L	337_A	0.677	0.29
212_S	281_E	0.676	0.29
174_I	355_A	0.675	0.29
291_Y	294_K	0.674	0.29
174_I	359_G	0.673	0.29
410_H	420_L	0.672	0.29
409_A	424_N	0.671	0.29
129_E	211_P	0.670	0.29
359_G	430_N	0.667	0.29
202_V	222_A	0.666	0.29
162_K	302_A	0.664	0.29
145_L	234_V	0.663	0.28
354_K	358_D	0.663	0.28
154_W	252_G	0.662	0.28
343_H	378_Y	0.662	0.28
224_I	405_M	0.661	0.28
95_K	165_F	0.660	0.28
425_L	431_P	0.660	0.28
155_V	303_K	0.659	0.28
199_V	359_G	0.658	0.28
204_S	351_L	0.658	0.28
149_M	256_R	0.657	0.28
352_F	418_Q	0.657	0.28
120_T	212_S	0.654	0.28
209_D	239_H	0.653	0.28
278_L	281_E	0.653	0.28
140_V	153_F	0.651	0.28
105_S	119_T	0.648	0.28
401_L	405_M	0.644	0.27
195_L	425_L	0.643	0.27
336_I	364_M	0.643	0.27
315_P	398_E	0.642	0.27
171_G	404_L	0.641	0.27
129_E	134_A	0.641	0.27
219_L	374_N	0.640	0.27
162_K	192_L	0.639	0.27
318_D	426_V	0.637	0.27
307_R	332_R	0.637	0.27
254_K	403_K	0.637	0.27
259_H	377_V	0.636	0.27
205_Q	378_Y	0.636	0.27
204_S	209_D	0.636	0.27
205_Q	364_M	0.633	0.27
97_I	160_S	0.631	0.27
323_E	420_L	0.629	0.26
212_S	365_T	0.627	0.26
265_A	270_N	0.627	0.26
149_M	157_I	0.627	0.26
376_N	391_P	0.626	0.26
168_G	310_L	0.623	0.26
99_T	266_F	0.623	0.26
350_E	432_R	0.622	0.26
310_L	407_V	0.618	0.26
184_T	255_V	0.618	0.26
227_K	426_V	0.618	0.26
356_T	397_P	0.618	0.26
125_Q	332_R	0.617	0.26
248_L	276_K	0.617	0.26
206_R	240_A	0.617	0.26
88_N	357_K	0.616	0.26
315_P	403_K	0.616	0.26
94_I	134_A	0.616	0.26
224_I	352_F	0.615	0.26
226_A	233_E	0.613	0.26
213_S	286_M	0.612	0.26
309_F	331_I	0.612	0.26
90_A	419_N	0.609	0.25
154_W	387_A	0.608	0.25
204_S	411_E	0.606	0.25
110_E	383_Q	0.604	0.25
381_G	385_L	0.604	0.25
174_I	184_T	0.601	0.25
285_V	288_N	0.600	0.25
187_A	205_Q	0.599	0.25
363_G	417_I	0.599	0.25
322_L	355_A	0.599	0.25
159_E	284_L	0.598	0.25
214_D	257_K	0.597	0.25
174_I	422_I	0.596	0.25
94_I	150_K	0.595	0.25
222_A	338_G	0.592	0.24
345_S	398_E	0.590	0.24
159_E	389_V	0.588	0.24
139_E	220_L	0.586	0.24
102_T	146_S	0.586	0.24
148_N	270_N	0.584	0.24
153_F	157_I	0.583	0.24
226_A	274_L	0.583	0.24
175_A	249_V	0.582	0.24
207_S	263_R	0.582	0.24
94_I	279_W	0.578	0.24
320_D	339_T	0.578	0.24
311_L	331_I	0.575	0.24
97_I	199_V	0.575	0.24
204_S	366_T	0.575	0.24
166_D	416_K	0.575	0.24
89_G	174_I	0.574	0.23
261_S	393_E	0.574	0.23
202_V	238_M	0.572	0.23
100_G	340_G	0.571	0.23
222_A	326_L	0.569	0.23
404_L	417_I	0.568	0.23
161_V	249_V	0.568	0.23
298_T	337_A	0.567	0.23
177_G	266_F	0.567	0.23
343_H	388_G	0.567	0.23
178_T	257_K	0.566	0.23
105_S	172_I	0.566	0.23
104_V	120_T	0.566	0.23
420_L	426_V	0.565	0.23
157_I	230_P	0.564	0.23
407_V	421_M	0.564	0.23
335_I	373_V	0.564	0.23
379_T	383_Q	0.562	0.23
125_Q	292_I	0.561	0.23
149_M	259_H	0.560	0.23
106_R	122_E	0.557	0.23
153_F	156_K	0.556	0.23
104_V	141_L	0.555	0.22
104_V	126_F	0.555	0.22
206_R	239_H	0.553	0.22
215_S	219_L	0.553	0.22
341_L	364_M	0.552	0.22
130_I	353_R	0.548	0.22
139_E	164_A	0.548	0.22
323_E	334_I	0.547	0.22
357_K	413_D	0.547	0.22
100_G	178_T	0.546	0.22
91_K	135_K	0.546	0.22
201_L	397_P	0.546	0.22
223_V	345_S	0.544	0.22
116_P	370_F	0.544	0.22
137_D	167_E	0.544	0.22
414_L	417_I	0.543	0.22
409_A	420_L	0.541	0.22
217_I	221_S	0.540	0.22
396_L	399_V	0.540	0.22
101_G	214_D	0.540	0.22
313_Y	344_T	0.538	0.22
148_N	180_T	0.536	0.22
170_T	249_V	0.536	0.22
408_L	411_E	0.535	0.21
261_S	366_T	0.532	0.21
182_A	399_V	0.532	0.21
290_K	295_K	0.531	0.21
94_I	232_G	0.530	0.21
368_C	398_E	0.528	0.21
185_A	240_A	0.528	0.21
303_K	335_I	0.526	0.21
159_E	350_E	0.525	0.21
268_S	275_A	0.525	0.21
104_V	212_S	0.525	0.21
269_V	389_V	0.525	0.21
183_Y	253_V	0.525	0.21
415_E	419_N	0.524	0.21
147_E	339_T	0.522	0.21
316_G	387_A	0.522	0.21
267_Q	422_I	0.522	0.21
322_L	351_L	0.521	0.21
182_A	363_G	0.521	0.21
353_R	419_N	0.520	0.21
270_N	345_S	0.520	0.21
145_L	252_G	0.520	0.21
186_S	252_G	0.519	0.21
120_T	204_S	0.519	0.21
199_V	290_K	0.519	0.21
102_T	112_G	0.519	0.21
145_L	396_L	0.519	0.21
257_K	266_F	0.519	0.21
338_G	341_L	0.518	0.21
196_Q	362_I	0.517	0.21
190_F	406_W	0.516	0.21
371_G	400_A	0.515	0.20
367_Q	403_K	0.515	0.20
103_I	259_H	0.515	0.20
163_K	199_V	0.515	0.20
256_R	369_L	0.514	0.20
319_R	351_L	0.513	0.20
366_T	393_E	0.513	0.20
124_I	127_L	0.513	0.20
251_R	384_L	0.512	0.20
174_I	271_D	0.509	0.20
342_G	367_Q	0.508	0.20
161_V	170_T	0.508	0.20
109_Y	112_G	0.508	0.20
138_A	285_V	0.508	0.20
254_K	367_Q	0.507	0.20
280_K	322_L	0.506	0.20
236_V	286_M	0.505	0.20
279_W	308_V	0.505	0.20
332_R	411_E	0.504	0.20
239_H	250_H	0.504	0.20
387_A	415_E	0.503	0.20
312_Y	397_P	0.501	0.20
167_E	326_L	0.501	0.20
146_S	266_F	0.501	0.20
236_V	362_I	0.501	0.20
161_V	165_F	0.501	0.20
86_V	89_G	0.500	0.20

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2d6fA	2	0.9572	100	0.184	Contact Map
1zq1A	2	0.9685	100	0.195	Contact Map
1wlsA	2	0.732	100	0.353	Contact Map
4q0mA	2	0.7297	100	0.353	Contact Map
2himA	4	0.741	100	0.355	Contact Map
4r8kA	3	0.741	100	0.359	Contact Map
2wltA	4	0.7162	100	0.38	Contact Map
1nnsA	4	0.723	100	0.383	Contact Map
1wsaA	4	0.7095	100	0.383	Contact Map
1agxA	4	0.7207	100	0.385	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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