GREMLIN Database
RIFK - Riboflavin kinase
UniProt: Q97ZG3 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 175 (170)
Sequences: 167 (129)
Seq/√Len: 9.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
35_L39_I3.2750.95
47_I88_I3.2280.95
120_A140_L2.8590.91
72_N78_D2.8300.90
60_I111_Y2.8090.90
105_S124_Y2.8030.90
77_F135_A2.6630.87
16_E31_C2.5370.84
70_Q74_I2.3190.78
31_C35_L2.1600.73
66_Y143_T2.1260.72
15_G93_S2.1160.71
164_K167_D2.1100.71
74_I109_P2.0880.70
18_I41_S1.9800.66
45_I140_L1.8670.61
60_I144_H1.8490.60
53_S59_K1.8370.60
156_H159_E1.8340.60
123_L148_V1.8180.59
16_E71_I1.8150.59
158_R165_D1.7260.55
29_S38_A1.7030.54
47_I62_L1.7030.54
124_Y153_S1.6940.53
36_K86_I1.6860.53
71_I123_L1.6770.53
113_Q124_Y1.6610.52
137_V145_P1.6420.51
118_L139_P1.6380.51
31_C118_L1.6350.51
118_L151_I1.6060.49
105_S122_K1.5720.48
136_V163_L1.5670.48
5_K18_I1.5660.48
59_K121_V1.5580.47
47_I121_V1.5560.47
68_K78_D1.4930.44
88_I143_T1.4870.44
105_S126_A1.4850.44
134_A154_P1.4810.44
31_C144_H1.4520.42
116_R141_R1.4410.42
20_L115_D1.4130.41
86_I142_T1.3700.39
100_L173_V1.3420.38
110_E151_I1.3410.38
125_P134_A1.3360.37
8_V169_V1.3340.37
16_E38_A1.3330.37
12_S47_I1.3310.37
118_L144_H1.3250.37
3_D169_V1.3230.37
51_I163_L1.3150.36
70_Q123_L1.2990.36
157_L161_L1.2650.34
88_I123_L1.2600.34
80_F164_K1.2500.34
110_E134_A1.2420.33
16_E70_Q1.2360.33
69_S89_Y1.2350.33
55_L145_P1.2310.33
87_V144_H1.2260.33
88_I97_R1.2210.33
38_A151_I1.2120.32
16_E32_L1.2030.32
28_L139_P1.2030.32
12_S17_I1.2020.32
100_L118_L1.1890.31
16_E35_L1.1770.31
77_F137_V1.1760.31
75_M165_D1.1680.30
106_L152_I1.1470.30
39_I143_T1.1410.29
4_D55_L1.1380.29
87_V134_A1.1330.29
35_L121_V1.1270.29
77_F145_P1.1200.29
31_C38_A1.1170.29
106_L153_S1.1150.28
62_L140_L1.1140.28
64_M69_S1.1110.28
109_P137_V1.1070.28
60_I118_L1.0970.28
52_V144_H1.0900.28
3_D6_L1.0800.27
159_E171_I1.0760.27
14_E25_E1.0510.26
159_E164_K1.0470.26
21_T32_L1.0340.26
11_I122_K1.0140.25
31_C139_P1.0060.25
1_M15_G1.0030.24
157_L165_D1.0010.24
44_S171_I0.9960.24
20_L30_S0.9940.24
123_L137_V0.9880.24
120_A139_P0.9750.24
120_A132_T0.9680.23
52_V87_V0.9640.23
158_R163_L0.9580.23
2_E24_G0.9440.23
20_L24_G0.9400.22
23_E169_V0.9290.22
9_R16_E0.9210.22
134_A151_I0.9180.22
5_K108_V0.8990.21
61_F111_Y0.8930.21
27_F129_N0.8930.21
110_E118_L0.8900.21
167_D171_I0.8890.21
89_Y149_I0.8890.21
142_T173_V0.8870.21
151_I163_L0.8840.21
150_E158_R0.8760.20
26_R37_D0.8730.20
162_N167_D0.8660.20
55_L77_F0.8660.20
61_F118_L0.8520.20
86_I172_E0.8480.20
86_I151_I0.8410.19
89_Y143_T0.8410.19
110_E120_A0.8270.19
18_I142_T0.8260.19
14_E35_L0.8250.19
88_I118_L0.8240.19
122_K138_I0.8230.19
14_E39_I0.8190.19
57_E79_P0.8180.19
66_Y113_Q0.8040.18
44_S103_S0.8000.18
16_E139_P0.7990.18
31_C39_I0.7950.18
52_V148_V0.7860.18
3_D138_I0.7780.18
68_K72_N0.7770.18
61_F117_V0.7700.17
118_L145_P0.7650.17
14_E80_F0.7650.17
62_L86_I0.7610.17
9_R88_I0.7600.17
16_E147_S0.7560.17
122_K159_E0.7550.17
51_I123_L0.7490.17
31_C70_Q0.7480.17
88_I121_V0.7360.17
105_S118_L0.7290.16
2_E13_K0.7250.16
30_S34_S0.7230.16
31_C59_K0.7220.16
137_V163_L0.7210.16
46_E72_N0.7200.16
106_L171_I0.7160.16
117_V141_R0.7070.16
64_M153_S0.7060.16
135_A145_P0.7030.16
121_V148_V0.7000.16
106_L123_L0.6960.16
61_F110_E0.6960.16
77_F123_L0.6940.16
86_I162_N0.6940.16
67_Y119_G0.6890.15
35_L70_Q0.6760.15
45_I120_A0.6740.15
113_Q123_L0.6730.15
23_E171_I0.6710.15
139_P161_L0.6700.15
125_P128_I0.6690.15
150_E157_L0.6640.15
25_E125_P0.6590.15
6_L32_L0.6570.15
46_E80_F0.6550.15
18_I53_S0.6540.15
20_L23_E0.6540.15
11_I45_I0.6520.15
71_I113_Q0.6510.15
80_F163_L0.6490.15
80_F112_K0.6480.15
90_D147_S0.6470.14
64_M97_R0.6440.14
45_I149_I0.6430.14
80_F156_H0.6420.14
3_D103_S0.6420.14
154_P161_L0.6380.14
62_L137_V0.6370.14
16_E118_L0.6350.14
126_A140_L0.6310.14
47_I129_N0.6310.14
110_E115_D0.6240.14
61_F143_T0.6220.14
63_S76_G0.6210.14
22_E26_R0.6110.14
70_Q117_V0.6110.14
23_E164_K0.6100.14
122_K160_K0.6100.14
66_Y70_Q0.6090.14
2_E7_I0.6060.14
31_C34_S0.6060.14
105_S125_P0.6010.13
3_D8_V0.6010.13
37_D153_S0.6010.13
51_I115_D0.6000.13
100_L171_I0.6000.13
74_I135_A0.6000.13
58_G67_Y0.5970.13
77_F109_P0.5960.13
20_L68_K0.5960.13
75_M108_V0.5930.13
13_K167_D0.5920.13
14_E53_S0.5920.13
55_L135_A0.5920.13
9_R71_I0.5900.13
154_P173_V0.5780.13
6_L147_S0.5750.13
59_K155_F0.5750.13
149_I167_D0.5710.13
59_K139_P0.5680.13
15_G139_P0.5660.13
80_F114_K0.5640.13
68_K120_A0.5610.13
10_I55_L0.5610.13
33_S99_L0.5610.13
60_I70_Q0.5600.13
2_E9_R0.5580.13
119_G141_R0.5570.13
56_G152_I0.5570.13
22_E151_I0.5540.13
13_K146_K0.5530.12
144_H147_S0.5530.12
84_L163_L0.5520.12
124_Y149_I0.5480.12
64_M117_V0.5460.12
18_I26_R0.5440.12
3_D148_V0.5430.12
31_C61_F0.5430.12
100_L129_N0.5430.12
46_E170_T0.5420.12
35_L143_T0.5420.12
29_S35_L0.5370.12
4_D19_R0.5360.12
72_N128_I0.5330.12
77_F136_V0.5290.12
58_G141_R0.5260.12
64_M88_I0.5260.12
2_E90_D0.5240.12
5_K51_I0.5200.12
19_R43_H0.5150.12
22_E34_S0.5130.12
28_L39_I0.5120.12
6_L46_E0.5100.12
103_S114_K0.5090.12
74_I106_L0.5080.12
1_M9_R0.5070.12
28_L165_D0.5010.11
131_L171_I0.5000.11
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2oynA 2 0.7371 100 0.351 Contact Map
3ctaA 2 0.8171 99.8 0.732 Contact Map
2p8tA 1 0.6571 99.3 0.818 Contact Map
2dqlA 2 0.2514 95.4 0.916 Contact Map
4l9nA 2 0.3829 93.9 0.923 Contact Map
2obpA 1 0.1829 93.6 0.924 Contact Map
3f8bA 2 0.2743 93.1 0.925 Contact Map
2qvoA 2 0.2171 93.1 0.925 Contact Map
2zfwA 2 0.2343 93 0.925 Contact Map
1xmaA 2 0.2686 93 0.926 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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