GREMLIN Database
Q97ZE5 - 3-hydroxyisobutyrate dehydrogenase
UniProt: Q97ZE5 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 289 (276)
Sequences: 11402 (7257)
Seq/√Len: 436.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
144_N148_K3.7021.00
72_K100_S3.4511.00
137_K141_D3.3471.00
27_L48_V3.1931.00
111_T139_K2.9551.00
40_V47_G2.9091.00
111_T142_I2.7831.00
232_K272_E2.3921.00
141_D144_N2.2671.00
49_Q53_D2.2381.00
36_I47_G2.2271.00
105_I110_G2.1831.00
4_G54_L2.1801.00
20_A45_V2.1721.00
37_E41_K2.1471.00
86_I143_V2.1041.00
24_K149_E1.9921.00
13_Y38_S1.9841.00
50_N53_D1.9691.00
209_R214_E1.9551.00
137_K156_Y1.9521.00
79_I105_I1.9401.00
194_E197_Q1.9181.00
111_T136_P1.8871.00
63_T87_L1.8761.00
96_T247_N1.8541.00
25_L46_N1.8451.00
18_N21_K1.8441.00
82_M85_K1.8341.00
22_A149_E1.7881.00
244_E247_N1.7791.00
232_K266_E1.7451.00
188_G198_V1.7421.00
32_T35_K1.7371.00
102_A160_N1.7351.00
34_E37_E1.7231.00
75_V87_L1.7211.00
106_E136_P1.7071.00
136_P139_K1.6841.00
103_K107_S1.6831.00
131_V150_T1.6631.00
33_Q37_E1.6611.00
81_L85_K1.6481.00
28_V45_V1.6401.00
270_Y273_A1.6361.00
64_M90_M1.6211.00
86_I139_K1.6191.00
104_R107_S1.5851.00
113_F135_G1.5831.00
131_V151_A1.5631.00
102_A106_E1.5531.00
140_F144_N1.5521.00
100_S104_R1.5381.00
140_F156_Y1.5151.00
115_A131_V1.5041.00
113_F143_V1.4991.00
54_L58_S1.4891.00
18_N127_K1.4871.00
62_I90_M1.4771.00
196_Q200_N1.4681.00
67_D70_A1.4631.00
52_K56_E1.4571.00
27_L46_N1.4561.00
218_P222_K1.4491.00
122_V126_Q1.4361.00
68_D97_L1.4161.00
176_S180_A1.4121.00
27_L57_S1.4091.00
121_S125_E1.4061.00
37_E40_V1.4051.00
260_E263_K1.3971.00
157_M166_S1.3951.00
231_T265_A1.3901.00
168_L240_I1.3801.00
189_I195_P1.3681.00
256_S260_E1.3641.00
257_L260_E1.3601.00
232_K271_S1.3411.00
98_S112_M1.3381.00
259_L263_K1.3321.00
33_Q49_Q1.3241.00
128_K152_S1.3141.00
57_S82_M1.3141.00
86_I142_I1.3131.00
99_I160_N1.3081.00
79_I108_N1.3051.00
59_D82_M1.2851.00
105_I112_M1.2821.00
113_F140_F1.2741.00
14_R125_E1.2731.00
88_I143_V1.2681.00
273_A280_E1.2621.00
64_M117_V1.2561.00
203_T214_E1.2341.00
60_V86_I1.2341.00
21_K125_E1.2341.00
235_R266_E1.2281.00
246_Q256_S1.2281.00
4_G58_S1.2281.00
17_A125_E1.2221.00
186_N190_R1.2131.00
136_P159_P1.2081.00
245_A252_T1.2041.00
16_A26_N1.2011.00
77_P80_P1.1971.00
85_K110_G1.1931.00
69_Y73_S1.1891.00
81_L110_G1.1851.00
175_A234_M1.1791.00
143_V147_A1.1791.00
99_I165_Y1.1751.00
60_V146_I1.1721.00
5_L15_I1.1601.00
80_P108_N1.1581.00
72_K104_R1.1561.00
175_A238_L1.1521.00
5_L16_A1.1491.00
243_R247_N1.1471.00
27_L58_S1.1451.00
90_M131_V1.1441.00
94_S97_L1.1391.00
38_S41_K1.1261.00
87_L105_I1.1231.00
20_A44_K1.1221.00
5_L62_I1.1171.00
251_I256_S1.1161.00
65_L74_V1.1161.00
191_A248_L1.1131.00
39_F45_V1.1061.00
159_P162_M1.0921.00
17_A21_K1.0841.00
68_D94_S1.0771.00
242_M256_S1.0741.00
104_R108_N1.0651.00
72_K97_L1.0651.00
90_M150_T1.0641.00
10_I121_S1.0601.00
235_R263_K1.0551.00
99_I103_K1.0551.00
133_V140_F1.0541.00
30_D35_K1.0481.00
62_I146_I1.0411.00
102_A136_P1.0331.00
239_E259_L1.0291.00
52_K77_P1.0281.00
148_K154_V1.0231.00
48_V53_D1.0211.00
103_K106_E1.0171.00
81_L87_L1.0161.00
173_L184_A1.0111.00
33_Q47_G1.0061.00
48_V54_L1.0021.00
243_R246_Q1.0021.00
114_D161_G1.0011.00
231_T266_E0.9981.00
197_Q200_N0.9911.00
156_Y159_P0.9851.00
75_V78_L0.9851.00
76_E80_P0.9801.00
113_F133_V0.9801.00
70_A73_S0.9671.00
62_I88_I0.9621.00
169_V184_A0.9481.00
211_P215_L0.9431.00
185_Y199_V0.9431.00
169_V188_G0.9421.00
132_L201_I0.9411.00
31_R70_A0.9401.00
239_E243_R0.9381.00
75_V105_I0.9341.00
83_K109_G0.9301.00
141_D156_Y0.9261.00
118_I170_N0.9261.00
229_F233_H0.9181.00
94_S243_R0.9171.00
124_V129_L0.9111.00
71_V93_I0.9091.00
176_S254_V0.9071.00
157_M162_M0.9051.00
135_G140_F0.9041.00
190_R250_I0.9041.00
97_L100_S0.9031.00
76_E104_R0.9021.00
199_V217_A0.9001.00
34_E38_S0.9001.00
86_I111_T0.9001.00
72_K101_L0.8991.00
273_A277_S0.8991.00
8_L39_F0.8981.00
18_N150_T0.8971.00
203_T213_T0.8941.00
263_K267_A0.8941.00
199_V203_T0.8891.00
118_I132_L0.8871.00
5_L26_N0.8871.00
239_E263_K0.8861.00
165_Y191_A0.8851.00
266_E271_S0.8831.00
13_Y17_A0.8801.00
157_M201_I0.8771.00
8_L28_V0.8671.00
145_D148_K0.8641.00
53_D56_E0.8601.00
98_S164_L0.8591.00
139_K142_I0.8561.00
27_L54_L0.8541.00
165_Y244_E0.8541.00
93_I97_L0.8541.00
235_R259_L0.8531.00
270_Y280_E0.8501.00
15_I150_T0.8501.00
19_L62_I0.8471.00
35_K38_S0.8431.00
60_V142_I0.8371.00
179_A254_V0.8311.00
100_S103_K0.8281.00
88_I115_A0.8241.00
96_T99_I0.8181.00
99_I161_G0.8121.00
114_D163_G0.8111.00
166_S193_L0.8091.00
196_Q222_K0.8061.00
226_S273_A0.8021.00
265_A277_S0.8021.00
203_T209_R0.8021.00
230_A272_E0.8021.00
51_T55_V0.8001.00
61_I78_L0.8001.00
63_T71_V0.7971.00
230_A233_H0.7901.00
172_L255_S0.7901.00
137_K159_P0.7891.00
254_V258_V0.7821.00
134_G163_G0.7811.00
177_Y210_S0.7801.00
66_A70_A0.7761.00
3_I24_K0.7751.00
139_K143_V0.7721.00
92_T240_I0.7671.00
225_Y280_E0.7671.00
177_Y181_I0.7671.00
89_D112_M0.7621.00
264_M268_L0.7611.00
140_F154_V0.7581.00
172_L241_I0.7571.00
217_A220_L0.7571.00
190_R248_L0.7551.00
264_M281_V0.7531.00
111_T138_D0.7511.00
182_A278_V0.7491.00
15_I90_M0.7491.00
242_M259_L0.7491.00
147_A154_V0.7461.00
31_R35_K0.7451.00
60_V88_I0.7421.00
28_V36_I0.7381.00
144_N154_V0.7341.00
244_E248_L0.7341.00
181_I213_T0.7331.00
120_T123_F0.7321.00
63_T75_V0.7311.00
231_T277_S0.7311.00
65_L70_A0.7281.00
173_L181_I0.7271.00
15_I129_L0.7261.00
188_G193_L0.7261.00
6_I29_Y0.7231.00
265_A270_Y0.7221.00
113_F139_K0.7211.00
5_L64_M0.7201.00
19_L26_N0.7131.00
33_Q36_I0.7101.00
79_I110_G0.7101.00
3_I146_I0.7091.00
263_K266_E0.7081.00
101_L112_M0.7071.00
196_Q221_L0.7061.00
24_K146_I0.7051.00
217_A221_L0.7041.00
246_Q259_L0.7031.00
15_I64_M0.7011.00
155_V201_I0.7011.00
186_N189_I0.6951.00
20_A26_N0.6901.00
268_L281_V0.6871.00
265_A281_V0.6841.00
264_M267_A0.6771.00
6_I51_T0.6771.00
61_I81_L0.6751.00
261_L264_M0.6751.00
53_D57_S0.6711.00
36_I40_V0.6701.00
177_Y212_T0.6691.00
138_D141_D0.6691.00
3_I19_L0.6681.00
97_L101_L0.6641.00
65_L71_V0.6631.00
173_L180_A0.6631.00
153_L200_N0.6611.00
115_A133_V0.6591.00
133_V147_A0.6551.00
165_Y193_L0.6510.99
153_L204_T0.6510.99
52_K76_E0.6480.99
79_I104_R0.6480.99
82_M110_G0.6480.99
71_V91_S0.6450.99
166_S198_V0.6450.99
13_Y39_F0.6430.99
187_F252_T0.6420.99
173_L177_Y0.6410.99
52_K80_P0.6410.99
73_S77_P0.6400.99
112_M160_N0.6400.99
277_S280_E0.6370.99
134_G156_Y0.6310.99
260_E264_M0.6310.99
173_L202_L0.6290.99
169_V187_F0.6290.99
123_F128_K0.6280.99
71_V89_D0.6270.99
168_L241_I0.6240.99
16_A28_V0.6230.99
73_S76_E0.6230.99
144_N156_Y0.6190.99
17_A20_A0.6190.99
219_K224_D0.6180.99
178_V275_F0.6180.99
137_K140_F0.6180.99
193_L198_V0.6140.99
176_S258_V0.6130.99
71_V97_L0.6130.99
147_A151_A0.6110.99
93_I98_S0.6090.99
242_M246_Q0.6070.99
239_E246_Q0.6060.99
6_I54_L0.6060.99
94_S240_I0.6050.99
30_D33_Q0.6030.99
37_E47_G0.6010.99
202_L208_A0.6000.99
9_G66_A0.6000.99
133_V154_V0.5990.99
14_R121_S0.5990.99
93_I164_L0.5980.99
115_A147_A0.5980.99
11_M15_I0.5960.99
54_L57_S0.5930.99
69_Y72_K0.5930.99
211_P214_E0.5930.99
58_S61_I0.5930.99
235_R239_E0.5880.99
29_Y51_T0.5860.99
51_T54_L0.5820.99
199_V221_L0.5800.99
30_D70_A0.5800.99
251_I264_M0.5790.99
83_K108_N0.5790.99
88_I147_A0.5740.99
260_E267_A0.5730.99
63_T89_D0.5720.99
157_M197_Q0.5720.99
61_I85_K0.5710.99
8_L16_A0.5690.99
75_V101_L0.5640.99
146_I149_E0.5640.99
46_N57_S0.5640.99
78_L81_L0.5620.98
60_V143_V0.5590.98
71_V101_L0.5590.98
36_I39_F0.5570.98
195_P221_L0.5570.98
123_F126_Q0.5570.98
95_P165_Y0.5540.98
224_D227_T0.5490.98
212_T216_K0.5480.98
162_M165_Y0.5480.98
182_A220_L0.5460.98
234_M262_Y0.5430.98
138_D142_I0.5420.98
62_I150_T0.5410.98
254_V257_L0.5410.98
152_S204_T0.5410.98
186_N250_I0.5380.98
137_K144_N0.5340.98
51_T78_L0.5340.98
52_K55_V0.5330.98
36_I49_Q0.5310.98
96_T100_S0.5270.98
216_K219_K0.5240.98
106_E111_T0.5240.98
14_R127_K0.5230.98
40_V45_V0.5220.98
18_N22_A0.5220.98
185_Y217_A0.5210.98
238_L262_Y0.5190.97
59_D85_K0.5180.97
145_D149_E0.5170.97
139_K160_N0.5160.97
89_D98_S0.5150.97
174_L212_T0.5140.97
169_V198_V0.5140.97
172_L187_F0.5140.97
66_A233_H0.5130.97
216_K274_D0.5110.97
219_K276_I0.5110.97
4_G27_L0.5100.97
30_D36_I0.5080.97
62_I147_A0.5060.97
74_V77_P0.5060.97
155_V197_Q0.5050.97
216_K220_L0.5040.97
130_V206_S0.5040.97
207_S212_T0.5000.97
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3dojA 3 0.9827 100 0.357 Contact Map
3zgyA 2 0.9758 100 0.357 Contact Map
3obbA 4 0.9792 100 0.359 Contact Map
3pefA 5 0.9758 100 0.361 Contact Map
3pduA 4 0.9758 100 0.361 Contact Map
3ckyA 4 0.9931 100 0.362 Contact Map
1vpdA 1 0.9931 100 0.365 Contact Map
4gbjA 2 0.9931 100 0.366 Contact Map
4oqzA 2 0.9723 100 0.368 Contact Map
3g0oA 3 0.9689 100 0.368 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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