GREMLIN Database
Q97ZD2 - Alpha amylase
UniProt: Q97ZD2 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 447 (386)
Sequences: 322 (198)
Seq/√Len: 10.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
102_A109_F8.1671.00
159_K294_E5.0061.00
56_T97_L4.1210.99
83_F87_A4.1120.99
149_T172_M4.0350.99
56_T98_F3.9300.99
55_A265_L3.9170.99
56_T248_Y3.9120.99
61_S266_K3.8980.99
188_N204_N3.8500.98
158_K298_T3.5570.97
44_F87_A3.4190.97
149_T283_K3.2760.96
140_K146_Y3.1910.95
172_M286_Y3.1800.95
98_F248_Y3.1740.95
312_L321_D3.0450.93
73_V279_M3.0440.93
165_V198_I3.0100.93
175_S299_T2.9900.93
112_Q151_E2.7930.90
50_N256_R2.7170.89
88_E138_T2.7020.88
200_M293_I2.6330.87
17_R31_E2.4890.84
59_I77_F2.4380.82
85_E114_Y2.4130.82
272_L277_V2.4010.81
162_V193_L2.3600.80
250_T253_E2.3120.79
96_E99_Q2.0510.69
76_F282_P2.0410.69
84_L142_Y2.0380.69
365_H368_F2.0000.67
253_E302_A1.9980.67
34_F387_T1.9970.67
74_K284_E1.9690.66
302_A365_H1.9070.64
7_G245_F1.8730.62
250_T302_A1.8680.62
55_A59_I1.8300.60
253_E365_H1.8200.60
64_E100_Q1.7910.58
220_W233_W1.7540.57
120_S161_I1.7510.57
124_D328_E1.7490.57
320_Y382_F1.6950.54
364_V368_F1.6940.54
234_I272_L1.6940.54
134_M343_R1.6920.54
149_T286_Y1.6750.53
15_R249_E1.6730.53
150_F165_V1.6270.51
213_F352_Y1.6160.50
151_E246_V1.6150.50
254_H367_Y1.6100.50
254_H302_A1.6060.50
114_Y143_F1.5860.49
53_I97_L1.5740.49
226_T229_K1.5630.48
234_I277_V1.5620.48
341_V391_E1.5450.47
91_G94_V1.5420.47
218_P223_Y1.5310.47
282_P285_V1.5260.46
93_E337_E1.5200.46
154_E254_H1.5180.46
12_Q15_R1.5180.46
85_E138_T1.5130.46
165_V173_M1.5090.46
246_V255_H1.5030.45
19_D34_F1.4960.45
220_W225_L1.4940.45
154_E368_F1.4740.44
51_C98_F1.4680.44
231_A272_L1.4670.44
249_E253_E1.4630.43
303_D306_K1.4550.43
184_G189_Y1.4530.43
154_E349_D1.4490.43
10_V98_F1.4440.43
109_F145_Q1.4330.42
96_E216_S1.3990.41
74_K286_Y1.3970.41
349_D353_Y1.3970.41
47_I256_R1.3920.40
302_A364_V1.3870.40
166_E195_G1.3870.40
154_E249_E1.3840.40
254_H352_Y1.3810.40
241_V285_V1.3590.39
148_T171_K1.3390.38
203_R244_I1.3360.38
249_E352_Y1.3310.38
118_V157_T1.3250.37
154_E213_F1.3170.37
97_L248_Y1.3120.37
249_E368_F1.3030.36
84_L138_T1.2970.36
310_S357_G1.2960.36
251_F265_L1.2950.36
213_F254_H1.2680.35
254_H364_V1.2660.35
156_I300_S1.2630.35
35_F90_W1.2600.35
340_R344_Y1.2600.35
230_Y234_I1.2570.35
307_D357_G1.2570.35
74_K171_K1.2530.34
15_R353_Y1.2520.34
80_S90_W1.2410.34
303_D307_D1.2340.34
101_L104_T1.2330.34
210_D220_W1.2320.33
44_F83_F1.2290.33
121_L156_I1.2250.33
247_D251_F1.2250.33
232_D275_K1.2100.33
74_K148_T1.2050.32
15_R349_D1.1970.32
249_E349_D1.1850.32
223_Y234_I1.1810.31
58_I262_L1.1770.31
235_S276_G1.1760.31
129_K167_K1.1750.31
96_E161_I1.1730.31
247_D254_H1.1720.31
46_R203_R1.1720.31
253_E367_Y1.1660.31
159_K179_N1.1620.31
10_V51_C1.1500.30
213_F302_A1.1450.30
63_I106_K1.1450.30
10_V56_T1.1430.30
293_I369_N1.1420.30
40_N54_P1.1370.30
302_A310_S1.1340.30
17_R291_D1.1300.29
49_K93_E1.1270.29
265_L271_E1.1200.29
213_F253_E1.1170.29
51_C168_M1.1170.29
81_G187_P1.1040.28
15_R368_F1.1000.28
42_E231_A1.0960.28
355_Y359_G1.0950.28
111_A136_K1.0910.28
12_Q40_N1.0890.28
206_T230_Y1.0880.28
168_M300_S1.0830.28
43_I368_F1.0830.28
19_D32_D1.0790.28
147_P312_L1.0760.27
148_T283_K1.0750.27
15_R154_E1.0750.27
63_I107_V1.0740.27
302_A368_F1.0740.27
217_N232_D1.0670.27
55_A251_F1.0660.27
18_R230_Y1.0580.27
112_Q246_V1.0550.27
113_T352_Y1.0510.27
88_E142_Y1.0500.26
250_T367_Y1.0500.26
35_F363_E1.0480.26
99_Q132_V1.0400.26
7_G155_L1.0370.26
1_M234_I1.0350.26
250_T365_H1.0300.26
78_S113_T1.0290.26
7_G112_Q1.0240.26
13_P117_S1.0190.25
364_V367_Y1.0190.25
153_T260_G1.0180.25
247_D352_Y1.0180.25
378_F383_Y1.0100.25
77_F259_T0.9960.25
88_E95_I0.9940.25
209_D253_E0.9930.24
247_D253_E0.9930.24
249_E254_H0.9920.24
130_E343_R0.9920.24
58_I265_L0.9900.24
104_T107_V0.9900.24
162_V195_G0.9890.24
205_Y309_K0.9880.24
310_S365_H0.9870.24
53_I93_E0.9820.24
132_V293_I0.9810.24
253_E368_F0.9780.24
41_K70_G0.9650.24
122_W321_D0.9530.23
303_D310_S0.9510.23
203_R212_A0.9510.23
43_I153_T0.9490.23
170_F287_N0.9490.23
125_K160_D0.9430.23
319_A378_F0.9430.23
253_E352_Y0.9410.23
249_E302_A0.9400.23
140_K144_G0.9370.23
15_R260_G0.9360.23
84_L139_I0.9330.22
253_E364_V0.9320.22
46_R253_E0.9300.22
391_E394_S0.9280.22
357_G365_H0.9270.22
181_N298_T0.9270.22
335_G339_L0.9260.22
12_Q368_F0.9250.22
46_R352_Y0.9210.22
302_A352_Y0.9180.22
12_Q364_V0.9150.22
350_N370_A0.9140.22
84_L99_Q0.9100.22
82_T347_T0.9090.22
7_G151_E0.9090.22
151_E348_S0.9030.22
77_F98_F0.8980.21
155_L216_S0.8980.21
254_H368_F0.8980.21
220_W224_P0.8970.21
298_T316_M0.8930.21
313_G377_A0.8910.21
260_G353_Y0.8870.21
213_F249_E0.8860.21
213_F247_D0.8860.21
250_T254_H0.8860.21
3_R289_V0.8850.21
250_T349_D0.8810.21
278_E288_D0.8800.21
19_D22_W0.8800.21
57_N100_Q0.8750.21
20_F323_A0.8740.21
7_G246_V0.8740.21
193_L294_E0.8720.21
44_F286_Y0.8680.20
19_D387_T0.8630.20
42_E162_V0.8630.20
25_R32_D0.8600.20
159_K298_T0.8600.20
39_R332_R0.8590.20
137_D146_Y0.8550.20
348_S364_V0.8520.20
12_Q349_D0.8520.20
206_T222_Q0.8510.20
46_R254_H0.8470.20
113_T150_F0.8440.20
43_I249_E0.8430.20
161_I321_D0.8370.20
154_E310_S0.8360.20
88_E376_D0.8330.19
102_A145_Q0.8320.19
258_Q293_I0.8300.19
209_D255_H0.8300.19
337_E340_R0.8270.19
253_E310_S0.8260.19
247_D250_T0.8260.19
84_L269_P0.8220.19
233_W367_Y0.8200.19
324_V346_T0.8200.19
228_S378_F0.8180.19
58_I363_E0.8180.19
129_K164_E0.8160.19
79_I83_F0.8150.19
10_V254_H0.8130.19
305_E357_G0.8120.19
154_E302_A0.8110.19
383_Y387_T0.8080.19
111_A270_I0.8030.19
95_I144_G0.8010.19
375_I380_N0.8010.19
49_K385_I0.7990.18
153_T251_F0.7970.18
15_R213_F0.7970.18
61_S276_G0.7960.18
14_F82_T0.7960.18
225_L263_E0.7960.18
15_R352_Y0.7940.18
280_M285_V0.7940.18
295_I299_T0.7930.18
256_R347_T0.7920.18
43_I47_I0.7870.18
309_K357_G0.7870.18
210_D233_W0.7850.18
12_Q302_A0.7850.18
14_F89_R0.7840.18
196_H239_G0.7840.18
12_Q43_I0.7830.18
93_E292_E0.7820.18
324_V385_I0.7810.18
129_K132_V0.7800.18
226_T299_T0.7780.18
43_I154_E0.7780.18
154_E353_Y0.7760.18
6_V378_F0.7740.18
17_R23_N0.7730.18
213_F365_H0.7730.18
127_E343_R0.7730.18
304_I324_V0.7720.18
115_Y343_R0.7700.18
117_S260_G0.7700.18
112_Q150_F0.7680.18
62_S262_L0.7680.18
359_G392_E0.7660.18
213_F250_T0.7630.18
286_Y337_E0.7610.17
302_A367_Y0.7580.17
23_N84_L0.7580.17
120_S388_F0.7570.17
86_Q302_A0.7560.17
149_T276_G0.7550.17
6_V363_E0.7520.17
117_S248_Y0.7500.17
385_I391_E0.7480.17
153_T253_E0.7480.17
19_D24_P0.7460.17
217_N378_F0.7450.17
302_A349_D0.7430.17
247_D302_A0.7430.17
60_L150_F0.7410.17
249_E353_Y0.7410.17
19_D346_T0.7400.17
20_F378_F0.7400.17
226_T353_Y0.7390.17
194_K197_E0.7370.17
210_D214_R0.7370.17
51_C54_P0.7360.17
228_S238_E0.7340.17
167_K240_N0.7330.17
196_H341_V0.7330.17
54_P252_G0.7310.17
177_G233_W0.7310.17
88_E285_V0.7300.17
15_R253_E0.7300.17
111_A170_F0.7300.17
100_Q136_K0.7270.17
254_H365_H0.7210.16
158_K161_I0.7210.16
144_G279_M0.7180.16
70_G173_M0.7130.16
137_D385_I0.7120.16
77_F248_Y0.7120.16
157_T371_F0.7120.16
55_A282_P0.7100.16
17_R192_K0.7090.16
253_E349_D0.7070.16
13_P204_N0.7060.16
160_D335_G0.7050.16
12_Q249_E0.7050.16
4_V384_A0.7040.16
35_F106_K0.7030.16
251_F369_N0.7010.16
178_T282_P0.7010.16
139_I145_Q0.7000.16
15_R43_I0.6990.16
344_Y378_F0.6990.16
114_Y187_P0.6980.16
79_I84_L0.6960.16
75_Y324_V0.6950.16
307_D310_S0.6950.16
15_R86_Q0.6940.16
81_G114_Y0.6940.16
22_W104_T0.6940.16
133_K211_I0.6920.16
4_V167_K0.6910.16
17_R277_V0.6880.16
239_G341_V0.6870.16
65_R80_S0.6870.16
127_E353_Y0.6830.15
303_D365_H0.6790.15
209_D301_W0.6790.15
105_H306_K0.6770.15
246_V354_L0.6770.15
13_P349_D0.6770.15
89_R228_S0.6770.15
15_R365_H0.6760.15
46_R302_A0.6750.15
125_K331_S0.6750.15
129_K165_V0.6730.15
9_E379_I0.6720.15
66_A76_F0.6710.15
116_H348_S0.6700.15
214_R220_W0.6700.15
65_R270_I0.6690.15
225_L233_W0.6690.15
229_K233_W0.6670.15
188_N260_G0.6670.15
380_N386_S0.6670.15
15_R311_W0.6660.15
130_E277_V0.6650.15
120_S141_S0.6630.15
83_F299_T0.6600.15
205_Y247_D0.6600.15
212_A244_I0.6590.15
39_R43_I0.6590.15
13_P260_G0.6570.15
247_D349_D0.6560.15
153_T368_F0.6560.15
151_E255_H0.6540.15
227_A267_W0.6530.15
45_E84_L0.6520.15
182_L185_R0.6510.15
77_F276_G0.6510.15
79_I110_L0.6510.15
222_Q229_K0.6480.15
118_V262_L0.6460.15
43_I353_Y0.6450.15
84_L287_N0.6430.15
249_E365_H0.6410.14
234_I278_E0.6410.14
190_V331_S0.6390.14
58_I271_E0.6390.14
229_K352_Y0.6340.14
301_W311_W0.6330.14
253_E353_Y0.6320.14
180_R380_N0.6300.14
46_R339_L0.6300.14
13_P127_E0.6290.14
376_D379_I0.6280.14
43_I365_H0.6280.14
63_I149_T0.6270.14
307_D311_W0.6270.14
353_Y368_F0.6250.14
150_F155_L0.6240.14
355_Y374_P0.6240.14
247_D367_Y0.6220.14
135_H187_P0.6160.14
260_G349_D0.6150.14
46_R113_T0.6140.14
165_V170_F0.6140.14
138_T278_E0.6130.14
181_N241_V0.6120.14
89_R335_G0.6090.14
159_K162_V0.6090.14
10_V60_L0.6090.14
352_Y365_H0.6070.14
250_T353_Y0.6060.14
133_K137_D0.6060.14
159_K315_I0.6050.14
21_F25_R0.6030.14
92_R244_I0.6010.14
265_L340_R0.6000.14
45_E49_K0.6000.14
171_K287_N0.6000.14
98_F379_I0.5990.14
32_D179_N0.5970.14
183_N299_T0.5970.14
235_S277_V0.5970.14
113_T248_Y0.5930.13
6_V109_F0.5930.13
20_F31_E0.5920.13
117_S131_Q0.5910.13
4_V278_E0.5910.13
112_Q355_Y0.5910.13
113_T349_D0.5910.13
77_F84_L0.5910.13
210_D225_L0.5890.13
230_Y238_E0.5880.13
291_D318_W0.5880.13
4_V338_Y0.5870.13
358_H369_N0.5850.13
320_Y381_E0.5850.13
78_S301_W0.5850.13
154_E365_H0.5850.13
197_E291_D0.5840.13
89_R218_P0.5840.13
179_N185_R0.5820.13
22_W25_R0.5810.13
352_Y368_F0.5810.13
181_N316_M0.5810.13
116_H154_E0.5800.13
127_E260_G0.5800.13
50_N245_F0.5790.13
10_V77_F0.5780.13
159_K193_L0.5770.13
282_P295_I0.5760.13
61_S65_R0.5740.13
155_L202_F0.5740.13
91_G341_V0.5730.13
303_D347_T0.5720.13
6_V141_S0.5710.13
119_T131_Q0.5700.13
189_Y287_N0.5700.13
303_D357_G0.5690.13
269_P294_E0.5650.13
200_M217_N0.5650.13
122_W322_D0.5640.13
46_R212_A0.5630.13
205_Y303_D0.5600.13
113_T204_N0.5590.13
222_Q255_H0.5590.13
72_N106_K0.5580.13
140_K259_T0.5560.13
120_S296_A0.5560.13
182_L186_S0.5560.13
21_F319_A0.5560.13
272_L276_G0.5540.13
373_S377_A0.5540.13
5_I384_A0.5540.13
272_L395_K0.5530.13
267_W332_R0.5530.13
84_L202_F0.5530.13
21_F24_P0.5530.13
83_F216_S0.5500.13
130_E332_R0.5500.13
120_S159_K0.5490.13
23_N263_E0.5470.12
154_E364_V0.5470.12
303_D348_S0.5450.12
87_A384_A0.5450.12
221_D375_I0.5440.12
356_L377_A0.5440.12
47_I247_D0.5420.12
293_I326_R0.5420.12
303_D311_W0.5420.12
154_E247_D0.5410.12
47_I253_E0.5400.12
35_F117_S0.5400.12
318_W346_T0.5400.12
55_A88_E0.5390.12
82_T263_E0.5390.12
98_F102_A0.5380.12
105_H259_T0.5370.12
332_R339_L0.5370.12
141_S324_V0.5360.12
272_L285_V0.5360.12
154_E250_T0.5360.12
45_E196_H0.5350.12
13_P154_E0.5340.12
178_T300_S0.5340.12
97_L101_L0.5330.12
334_L339_L0.5320.12
55_A99_Q0.5320.12
230_Y336_N0.5320.12
136_K259_T0.5310.12
16_I259_T0.5300.12
249_E260_G0.5290.12
213_F349_D0.5270.12
66_A275_K0.5270.12
84_L164_E0.5270.12
250_T368_F0.5260.12
198_I258_Q0.5260.12
213_F251_F0.5250.12
356_L371_F0.5250.12
265_L294_E0.5240.12
254_H310_S0.5230.12
19_D383_Y0.5230.12
145_Q270_I0.5220.12
74_K280_M0.5210.12
145_Q324_V0.5210.12
80_S161_I0.5210.12
117_S127_E0.5200.12
193_L321_D0.5180.12
100_Q103_Y0.5170.12
244_I318_W0.5160.12
99_Q218_P0.5140.12
6_V203_R0.5140.12
94_V208_S0.5130.12
36_D107_V0.5120.12
59_I75_Y0.5120.12
63_I109_F0.5110.12
162_V342_W0.5090.12
316_M370_A0.5080.12
13_P353_Y0.5080.12
230_Y346_T0.5080.12
113_T247_D0.5070.12
2_K256_R0.5070.12
255_H325_R0.5070.12
151_E155_L0.5060.12
121_L304_I0.5050.12
36_D104_T0.5050.12
147_P170_F0.5040.12
99_Q103_Y0.5030.12
51_C77_F0.5020.12
84_L137_D0.5020.12
44_F325_R0.5020.12
175_S219_N0.5020.12
153_T310_S0.5000.12
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3n98A 1 0.8635 100 0.637 Contact Map
2b5dX 3 0.83 100 0.645 Contact Map
3p0bA 1 0.8389 100 0.646 Contact Map
1k1xA 2 0.8546 100 0.723 Contact Map
4cmrA 3 0.9262 100 0.77 Contact Map
4ly4A 3 0.5772 99.8 0.896 Contact Map
1o7dA 1 0.5481 99.7 0.904 Contact Map
3rxzA 3 0.5884 99.7 0.907 Contact Map
2vyoA 1 0.443 99.6 0.912 Contact Map
4m1bA 1 0.4385 99.6 0.916 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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