Q97Z67 - SULSO - GREMLIN Contacts

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Q97Z67 - Uncharacterized protein

UniProt:	Q97Z67	Sequences: MIKIIFVLEVNPADENEVFQIISESKKDNKKVGIRGFGSHTKREIKPDIMIITTEKLNNFEISENKVIADSGADVKKIREEALQKGLLFPSIYDGSIGGLLALNEMSSISTAYGTPWNFTEWVDFINTFGKIRWRAVIGSQGLFGVITKASLKLYEKPSKVFIYEREITDKKELKLELRKLIGLKPIALLVEYEGDNKAFEIHASYVTQYDLQSYSKDEGIPMISEVSDKNSYIVEISDFVEDFISIAERVSPYYMYGIYGVNLLKVYVTDDSLLRDLKYYPRNKPHPVFYKLKRVLDFYNIFA	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	304 (290)
Sequences:	9094 (6196)
Seq/√Len:	363.8

ECOLI - YDIJ GLCD YGCU GLCE
BACSU - GLCD YITY YVDP YGAK
HALSA - Q9HMR2
SULSO - Q97Z67 Q7LX43 Q97U60 Q97ZB9 Q97Y18 Q97U59 Q7LXI5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
101_L	150_A	4.452	1.00
170_D	173_E	3.793	1.00
285_K	288_P	3.620	1.00
15_E	124_D	3.458	1.00
124_D	149_K	3.275	1.00
172_K	291_Y	3.148	1.00
125_F	137_V	3.072	1.00
22_I	32_V	3.069	1.00
70_D	149_K	3.033	1.00
59_N	70_D	2.971	1.00
60_F	70_D	2.628	1.00
19_F	132_I	2.620	1.00
83_L	157_K	2.579	1.00
10_V	21_I	2.551	1.00
67_V	78_I	2.534	1.00
102_A	140_S	2.527	1.00
68_I	151_S	2.478	1.00
52_I	146_V	2.352	1.00
81_E	84_Q	2.346	1.00
35_R	38_G	2.223	1.00
87_L	154_L	2.216	1.00
73_A	78_I	2.207	1.00
68_I	149_K	2.178	1.00
24_E	28_D	2.178	1.00
76_K	80_E	2.174	1.00
60_F	149_K	2.164	1.00
15_E	132_I	2.144	1.00
62_I	81_E	2.132	1.00
137_V	145_G	2.110	1.00
74_D	77_K	2.083	1.00
67_V	152_L	2.078	1.00
13_A	56_K	2.072	1.00
77_K	80_E	2.069	1.00
58_N	77_K	2.062	1.00
63_S	66_K	2.037	1.00
15_E	148_T	2.004	1.00
13_A	17_E	2.001	1.00
16_N	20_Q	1.956	1.00
18_V	148_T	1.941	1.00
125_F	145_G	1.936	1.00
295_R	299_F	1.902	1.00
22_I	26_K	1.884	1.00
95_G	100_L	1.855	1.00
121_E	153_K	1.829	1.00
172_K	176_L	1.798	1.00
15_E	19_F	1.782	1.00
61_E	68_I	1.768	1.00
54_T	146_V	1.768	1.00
169_T	173_E	1.766	1.00
127_N	131_K	1.753	1.00
292_K	295_R	1.723	1.00
101_L	147_I	1.704	1.00
168_I	177_E	1.700	1.00
32_V	52_I	1.685	1.00
82_A	154_L	1.673	1.00
288_P	292_K	1.661	1.00
20_Q	23_S	1.654	1.00
197_N	201_E	1.648	1.00
11_N	56_K	1.648	1.00
120_T	138_I	1.626	1.00
10_V	50_M	1.623	1.00
173_E	176_L	1.595	1.00
124_D	148_T	1.593	1.00
64_E	85_K	1.573	1.00
124_D	132_I	1.545	1.00
14_D	17_E	1.544	1.00
69_A	97_I	1.538	1.00
102_A	147_I	1.531	1.00
24_E	30_K	1.527	1.00
22_I	146_V	1.524	1.00
18_V	146_V	1.487	1.00
119_F	155_Y	1.482	1.00
101_L	120_T	1.462	1.00
294_K	298_D	1.457	1.00
62_I	67_V	1.455	1.00
34_I	146_V	1.425	1.00
258_G	262_V	1.401	1.00
36_G	96_S	1.393	1.00
18_V	52_I	1.374	1.00
19_F	23_S	1.362	1.00
16_N	19_F	1.360	1.00
172_K	288_P	1.355	1.00
243_D	246_S	1.346	1.00
122_W	151_S	1.344	1.00
12_P	17_E	1.329	1.00
295_R	298_D	1.319	1.00
242_E	246_S	1.303	1.00
46_K	49_I	1.301	1.00
81_E	85_K	1.298	1.00
233_Y	237_I	1.284	1.00
21_I	52_I	1.278	1.00
54_T	57_L	1.270	1.00
249_E	252_S	1.269	1.00
260_Y	263_N	1.254	1.00
25_S	50_M	1.232	1.00
9_E	51_I	1.230	1.00
70_D	73_A	1.229	1.00
97_I	150_A	1.204	1.00
55_E	96_S	1.203	1.00
126_I	132_I	1.198	1.00
231_N	235_V	1.195	1.00
140_S	144_F	1.179	1.00
59_N	73_A	1.174	1.00
57_L	70_D	1.166	1.00
260_Y	268_Y	1.165	1.00
23_S	27_K	1.156	1.00
23_S	26_K	1.153	1.00
75_V	100_L	1.151	1.00
101_L	116_P	1.145	1.00
22_I	126_I	1.137	1.00
292_K	296_V	1.125	1.00
257_Y	260_Y	1.123	1.00
264_L	268_Y	1.122	1.00
282_P	285_K	1.117	1.00
201_E	205_S	1.117	1.00
12_P	21_I	1.111	1.00
261_G	265_L	1.108	1.00
76_K	92_I	1.106	1.00
76_K	94_D	1.079	1.00
110_S	115_T	1.071	1.00
198_K	201_E	1.068	1.00
18_V	54_T	1.063	1.00
235_V	239_D	1.059	1.00
69_A	78_I	1.052	1.00
15_E	126_I	1.050	1.00
8_L	50_M	1.046	1.00
35_R	51_I	1.045	1.00
218_D	221_I	1.040	1.00
75_V	95_G	1.031	1.00
19_F	126_I	1.027	1.00
137_V	147_I	1.022	1.00
268_Y	271_D	1.016	1.00
15_E	70_D	1.015	1.00
134_W	138_I	1.010	1.00
17_E	20_Q	1.003	1.00
199_A	203_H	0.998	1.00
7_V	48_D	0.997	1.00
291_Y	295_R	0.987	1.00
123_V	134_W	0.983	1.00
67_V	82_A	0.980	1.00
281_Y	289_V	0.977	1.00
132_I	148_T	0.975	1.00
230_K	233_Y	0.975	1.00
66_K	284_N	0.972	1.00
79_R	88_L	0.969	1.00
25_S	32_V	0.968	1.00
40_H	43_R	0.967	1.00
34_I	54_T	0.966	1.00
76_K	93_Y	0.965	1.00
265_L	268_Y	0.947	1.00
176_L	180_K	0.943	1.00
36_G	54_T	0.936	1.00
80_E	84_Q	0.926	1.00
90_P	119_F	0.916	1.00
97_I	152_L	0.911	1.00
79_R	82_A	0.911	1.00
77_K	81_E	0.909	1.00
11_N	55_E	0.906	1.00
63_S	68_I	0.899	1.00
262_V	266_K	0.899	1.00
82_A	85_K	0.897	1.00
86_G	157_K	0.897	1.00
15_E	57_L	0.895	1.00
289_V	293_L	0.892	1.00
225_S	228_S	0.888	1.00
221_I	225_S	0.884	1.00
288_P	291_Y	0.884	1.00
58_N	70_D	0.880	1.00
23_S	131_K	0.878	1.00
26_K	29_N	0.878	1.00
201_E	217_K	0.878	1.00
7_V	49_I	0.875	1.00
80_E	83_L	0.875	1.00
123_V	150_A	0.872	1.00
65_N	87_L	0.869	1.00
147_I	150_A	0.864	1.00
239_D	242_E	0.850	1.00
20_Q	24_E	0.848	1.00
12_P	54_T	0.847	1.00
258_G	261_G	0.847	1.00
79_R	157_K	0.845	1.00
259_I	263_N	0.843	1.00
31_K	49_I	0.842	1.00
17_E	21_I	0.836	1.00
265_L	269_V	0.835	1.00
78_I	152_L	0.834	1.00
18_V	126_I	0.833	1.00
195_G	198_K	0.830	1.00
126_I	148_T	0.825	1.00
233_Y	236_E	0.810	1.00
71_S	150_A	0.809	1.00
37_F	96_S	0.808	1.00
247_I	250_R	0.808	1.00
201_E	204_A	0.805	1.00
189_L	227_V	0.802	1.00
222_P	226_E	0.801	1.00
19_F	22_I	0.800	1.00
180_K	183_G	0.800	1.00
255_Y	258_G	0.797	1.00
263_N	266_K	0.797	1.00
11_N	53_T	0.793	1.00
123_V	147_I	0.792	1.00
241_V	245_I	0.791	1.00
127_N	133_R	0.789	1.00
260_Y	271_D	0.789	1.00
30_K	50_M	0.789	1.00
89_F	100_L	0.786	1.00
62_I	85_K	0.785	1.00
192_E	195_G	0.780	1.00
265_L	272_D	0.779	1.00
257_Y	261_G	0.777	1.00
279_K	293_L	0.776	1.00
160_K	231_N	0.774	1.00
260_Y	264_L	0.770	1.00
62_I	87_L	0.767	1.00
198_K	294_K	0.765	1.00
135_R	138_I	0.762	1.00
33_G	49_I	0.760	1.00
120_T	123_V	0.759	1.00
225_S	229_D	0.753	1.00
67_V	154_L	0.753	1.00
296_V	299_F	0.749	1.00
287_H	291_Y	0.749	1.00
264_L	275_L	0.748	1.00
101_L	138_I	0.746	1.00
197_N	200_F	0.741	1.00
221_I	226_E	0.740	1.00
118_N	135_R	0.740	1.00
264_L	274_L	0.739	1.00
88_L	157_K	0.737	1.00
200_F	204_A	0.736	1.00
12_P	56_K	0.734	1.00
88_L	158_P	0.733	1.00
222_P	225_S	0.733	1.00
241_V	244_F	0.732	1.00
176_L	291_Y	0.731	1.00
226_E	229_D	0.729	1.00
162_F	235_V	0.729	1.00
221_I	227_V	0.727	1.00
100_L	116_P	0.725	1.00
19_F	26_K	0.722	1.00
125_F	133_R	0.717	0.99
157_K	161_V	0.717	0.99
241_V	246_S	0.716	0.99
242_E	245_I	0.716	0.99
45_I	49_I	0.714	0.99
268_Y	272_D	0.713	0.99
54_T	71_S	0.712	0.99
119_F	153_K	0.712	0.99
89_F	116_P	0.711	0.99
272_D	275_L	0.711	0.99
267_V	272_D	0.710	0.99
248_A	251_V	0.709	0.99
79_R	83_L	0.709	0.99
127_N	137_V	0.708	0.99
190_L	193_Y	0.706	0.99
220_G	227_V	0.704	0.99
260_Y	266_K	0.701	0.99
215_Y	218_D	0.701	0.99
293_L	296_V	0.700	0.99
159_S	229_D	0.700	0.99
256_M	260_Y	0.699	0.99
254_Y	258_G	0.699	0.99
123_V	137_V	0.697	0.99
293_L	297_L	0.696	0.99
120_T	150_A	0.696	0.99
203_H	206_Y	0.695	0.99
195_G	199_A	0.693	0.99
78_I	97_I	0.692	0.99
180_K	184_L	0.691	0.99
60_F	68_I	0.691	0.99
78_I	89_F	0.688	0.99
268_Y	274_L	0.688	0.99
166_R	224_I	0.687	0.99
104_N	135_R	0.687	0.99
197_N	294_K	0.686	0.99
26_K	129_F	0.684	0.99
250_R	254_Y	0.682	0.99
221_I	228_S	0.681	0.99
15_E	18_V	0.680	0.99
197_N	219_E	0.678	0.99
39_S	94_D	0.677	0.99
239_D	243_D	0.677	0.99
21_I	50_M	0.677	0.99
75_V	92_I	0.677	0.99
87_L	156_E	0.675	0.99
268_Y	273_S	0.674	0.99
223_M	226_E	0.673	0.99
19_F	127_N	0.672	0.99
262_V	265_L	0.672	0.99
78_I	82_A	0.671	0.99
136_A	144_F	0.669	0.99
260_Y	265_L	0.665	0.99
203_H	207_V	0.662	0.99
265_L	271_D	0.660	0.99
67_V	81_E	0.650	0.99
246_S	249_E	0.649	0.99
231_N	234_I	0.647	0.99
16_N	132_I	0.645	0.99
223_M	227_V	0.645	0.99
172_K	287_H	0.645	0.99
166_R	245_I	0.645	0.99
205_S	208_T	0.644	0.99
271_D	275_L	0.643	0.99
219_E	222_P	0.642	0.99
188_A	191_V	0.641	0.99
247_I	251_V	0.641	0.99
271_D	274_L	0.640	0.99
188_A	192_E	0.640	0.99
261_G	264_L	0.638	0.99
256_M	267_V	0.638	0.99
75_V	91_S	0.636	0.99
232_S	236_E	0.635	0.99
171_K	290_F	0.629	0.99
263_N	268_Y	0.628	0.99
115_T	118_N	0.625	0.99
234_I	238_S	0.624	0.99
245_I	249_E	0.623	0.99
254_Y	257_Y	0.623	0.99
199_A	202_I	0.622	0.99
257_Y	263_N	0.621	0.99
24_E	50_M	0.621	0.99
22_I	128_T	0.617	0.98
230_K	236_E	0.617	0.98
215_Y	226_E	0.616	0.98
66_K	151_S	0.616	0.98
79_R	92_I	0.615	0.98
104_N	115_T	0.614	0.98
171_K	193_Y	0.613	0.98
236_E	239_D	0.613	0.98
213_Q	219_E	0.613	0.98
74_D	94_D	0.611	0.98
104_N	116_P	0.608	0.98
18_V	57_L	0.607	0.98
258_G	267_V	0.605	0.98
258_G	268_Y	0.603	0.98
256_M	263_N	0.602	0.98
123_V	138_I	0.601	0.98
216_S	219_E	0.596	0.98
195_G	294_K	0.591	0.98
69_A	152_L	0.589	0.98
101_L	152_L	0.587	0.98
21_I	24_E	0.586	0.98
160_K	234_I	0.586	0.98
255_Y	260_Y	0.584	0.98
40_H	44_E	0.584	0.98
17_E	56_K	0.584	0.98
257_Y	268_Y	0.584	0.98
91_S	94_D	0.583	0.98
40_H	103_L	0.583	0.98
32_V	146_V	0.582	0.98
220_G	224_I	0.580	0.98
190_L	194_E	0.579	0.98
74_D	96_S	0.577	0.98
21_I	146_V	0.577	0.98
176_L	179_R	0.576	0.98
89_F	152_L	0.576	0.98
25_S	30_K	0.575	0.98
175_K	290_F	0.575	0.98
267_V	271_D	0.575	0.98
118_N	153_K	0.572	0.98
291_Y	294_K	0.572	0.98
263_N	272_D	0.570	0.97
220_G	225_S	0.570	0.97
197_N	204_A	0.570	0.97
162_F	238_S	0.567	0.97
248_A	252_S	0.566	0.97
101_L	123_V	0.565	0.97
6_F	38_G	0.564	0.97
269_V	273_S	0.564	0.97
213_Q	218_D	0.562	0.97
228_S	234_I	0.560	0.97
22_I	131_K	0.558	0.97
261_G	272_D	0.557	0.97
242_E	248_A	0.556	0.97
214_S	217_K	0.556	0.97
214_S	220_G	0.556	0.97
194_E	200_F	0.555	0.97
215_Y	221_I	0.554	0.97
97_I	100_L	0.552	0.97
258_G	263_N	0.552	0.97
111_T	182_I	0.551	0.97
257_Y	275_L	0.548	0.97
89_F	119_F	0.548	0.97
67_V	73_A	0.548	0.97
179_R	182_I	0.547	0.97
267_V	275_L	0.545	0.97
219_E	226_E	0.545	0.97
171_K	175_K	0.545	0.97
86_G	159_S	0.543	0.97
73_A	77_K	0.542	0.97
24_E	27_K	0.541	0.97
69_A	150_A	0.541	0.97
122_W	134_W	0.541	0.97
88_L	155_Y	0.539	0.96
266_K	269_V	0.538	0.96
181_L	191_V	0.538	0.96
214_S	218_D	0.536	0.96
58_N	73_A	0.535	0.96
261_G	268_Y	0.534	0.96
229_D	233_Y	0.533	0.96
37_F	74_D	0.532	0.96
178_L	191_V	0.531	0.96
232_S	235_V	0.529	0.96
204_A	207_V	0.529	0.96
15_E	149_K	0.528	0.96
194_E	203_H	0.527	0.96
264_L	270_T	0.525	0.96
226_E	230_K	0.524	0.96
179_R	183_G	0.523	0.96
266_K	271_D	0.522	0.96
182_I	283_R	0.522	0.96
267_V	274_L	0.522	0.96
35_R	44_E	0.521	0.96
170_D	176_L	0.518	0.96
238_S	241_V	0.517	0.96
6_F	51_I	0.517	0.96
33_G	48_D	0.516	0.96
243_D	247_I	0.516	0.96
264_L	267_V	0.515	0.95
123_V	133_R	0.513	0.95
256_M	259_I	0.512	0.95
175_K	179_R	0.511	0.95
214_S	219_E	0.510	0.95
224_I	227_V	0.509	0.95
258_G	275_L	0.508	0.95
200_F	203_H	0.508	0.95
294_K	297_L	0.507	0.95
228_S	233_Y	0.507	0.95
136_A	139_G	0.506	0.95
255_Y	272_D	0.506	0.95
258_G	264_L	0.504	0.95
68_I	71_S	0.503	0.95
61_E	151_S	0.501	0.95
264_L	272_D	0.500	0.95

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3pm9A	5	0.9868	100	0.292	Contact Map
1f0xA	1	0.9638	100	0.296	Contact Map
2uuuA	2	0.9934	100	0.3	Contact Map
4bbyA	2	0.9737	100	0.314	Contact Map
2exrA	1	0.977	100	0.333	Contact Map
1w1oA	1	0.9704	100	0.335	Contact Map
4mlaA	1	0.9474	100	0.341	Contact Map
4o95A	1	0.9309	100	0.349	Contact Map
1wvfA	2	0.9868	100	0.363	Contact Map
4p8nA	1	0.9638	100	0.395	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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