Q97Z62 - SULSO - GREMLIN Contacts

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Q97Z62 - Partial transposase ISC1229

UniProt:	Q97Z62	Sequences: MVNKTKDTYNPLRELVSLYLFMKDAQTKQENRLRRALFLVNDEEKLSEERLEKFSKGDFSDVKLYQLEYTGTVLREIQVLARILLQTREELKEVGKMIESNVPQDHVLLTIPGIGKLAAGIIIGIVGDIRRFPRPESFVAYCGLDPVVERSGRIEVRKGISKRGNKYLRSLFYFLAETQYSRNPTLLKFYETHKDKLKGKKLYTALARKLARIVWSVWYNNKPYEVK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	227 (215)
Sequences:	4523 (3482)
Seq/√Len:	237.5

SULSO - Q97Z62 Q97Y06 Q97X29 Q97VB2 Q97YE1 Q97WZ5 Q97UQ6 Q97UF7 Q97X07 Q97X12 Q97Y59 Q97ZN4 Q97WC0 Q97XK2 Q97VS0 Q97X26 Q97XR9 Q97XV5 Q97WY7 Q7LXX5 Q97TX3 Q97XM7 Q97UU8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
75_R	97_M	2.908	1.00
138_F	211_A	2.757	1.00
193_H	196_K	2.716	1.00
79_V	90_E	2.661	1.00
126_V	138_F	2.476	1.00
107_V	223_P	2.400	1.00
142_C	168_L	2.389	1.00
26_Q	88_R	2.374	1.00
110_T	223_P	2.314	1.00
187_L	191_E	2.308	1.00
133_P	137_S	2.182	1.00
82_R	90_E	2.182	1.00
160_I	204_T	2.147	1.00
86_Q	90_E	2.145	1.00
122_I	214_V	2.136	1.00
25_A	28_K	2.081	1.00
25_A	32_R	1.931	1.00
83_I	86_Q	1.920	1.00
134_R	137_S	1.881	1.00
132_F	141_Y	1.847	1.00
144_L	207_A	1.839	1.00
198_K	201_K	1.834	1.00
208_R	212_R	1.818	1.00
111_I	214_V	1.751	1.00
107_V	217_V	1.741	1.00
142_C	211_A	1.731	1.00
150_R	155_E	1.727	1.00
121_I	171_L	1.725	1.00
192_T	196_K	1.698	1.00
194_K	200_K	1.694	1.00
83_I	90_E	1.693	1.00
114_I	119_A	1.677	1.00
54_F	58_D	1.662	1.00
108_L	214_V	1.657	1.00
25_A	83_I	1.640	1.00
222_K	225_E	1.632	1.00
33_L	84_L	1.629	1.00
23_K	91_L	1.624	1.00
174_F	177_E	1.612	1.00
97_M	100_S	1.598	1.00
19_Y	95_G	1.591	1.00
118_A	171_L	1.587	1.00
186_L	206_L	1.586	1.00
57_G	61_D	1.585	1.00
111_I	217_V	1.581	1.00
217_V	223_P	1.567	1.00
81_A	85_L	1.567	1.00
216_S	220_N	1.539	1.00
109_L	116_K	1.535	1.00
20_L	170_S	1.534	1.00
50_R	53_K	1.530	1.00
26_Q	91_L	1.519	1.00
107_V	110_T	1.509	1.00
27_T	31_N	1.486	1.00
215_W	219_Y	1.483	1.00
139_V	145_D	1.482	1.00
176_A	210_L	1.473	1.00
186_L	213_I	1.463	1.00
139_V	208_R	1.461	1.00
92_K	95_G	1.455	1.00
180_Y	190_Y	1.449	1.00
102_V	109_L	1.424	1.00
189_F	209_K	1.396	1.00
19_Y	23_K	1.393	1.00
190_Y	206_L	1.391	1.00
17_S	125_I	1.385	1.00
58_D	61_D	1.382	1.00
191_E	195_D	1.370	1.00
176_A	206_L	1.370	1.00
197_L	201_K	1.366	1.00
23_K	26_Q	1.366	1.00
99_E	116_K	1.361	1.00
111_I	210_L	1.325	1.00
82_R	89_E	1.322	1.00
82_R	86_Q	1.319	1.00
84_L	88_R	1.313	1.00
170_S	174_F	1.297	1.00
22_M	91_L	1.288	1.00
173_Y	207_A	1.285	1.00
118_A	175_L	1.269	1.00
33_L	77_I	1.261	1.00
125_I	165_N	1.259	1.00
175_L	210_L	1.255	1.00
189_F	193_H	1.236	1.00
176_A	207_A	1.230	1.00
26_Q	84_L	1.223	1.00
46_L	50_R	1.209	1.00
25_A	29_Q	1.194	1.00
24_D	28_K	1.178	1.00
108_L	119_A	1.158	1.00
86_Q	89_E	1.149	1.00
108_L	218_W	1.138	1.00
55_S	59_F	1.119	1.00
129_I	218_W	1.110	1.00
29_Q	33_L	1.107	1.00
193_H	202_L	1.104	1.00
30_E	48_E	1.103	1.00
95_G	99_E	1.102	1.00
51_L	58_D	1.101	1.00
194_K	199_G	1.092	1.00
97_M	101_N	1.089	1.00
21_F	32_R	1.088	1.00
54_F	57_G	1.077	1.00
79_V	93_E	1.061	1.00
173_Y	204_T	1.051	1.00
50_R	57_G	1.045	1.00
56_K	60_S	1.043	1.00
114_I	214_V	1.035	1.00
26_Q	30_E	1.032	1.00
54_F	61_D	1.028	1.00
160_I	169_R	1.024	1.00
172_F	211_A	1.018	1.00
35_R	39_L	1.016	1.00
79_V	97_M	1.015	1.00
51_L	54_F	1.011	1.00
193_H	198_K	1.008	1.00
194_K	202_L	1.003	1.00
95_G	98_I	1.003	1.00
151_S	155_E	1.002	1.00
54_F	59_F	0.998	1.00
216_S	219_Y	0.996	1.00
189_F	205_A	0.992	1.00
200_K	203_Y	0.979	1.00
16_V	167_Y	0.970	1.00
114_I	118_A	0.965	1.00
177_E	200_K	0.964	1.00
213_I	217_V	0.962	1.00
72_T	97_M	0.961	1.00
88_R	92_K	0.960	1.00
189_F	192_T	0.959	1.00
184_P	187_L	0.959	1.00
56_K	59_F	0.958	1.00
186_L	210_L	0.958	1.00
11_P	70_T	0.954	1.00
190_Y	202_L	0.949	1.00
20_L	24_D	0.944	1.00
137_S	140_A	0.939	1.00
173_Y	177_E	0.926	1.00
47_S	51_L	0.924	1.00
90_E	93_E	0.912	1.00
48_E	52_E	0.912	1.00
176_A	203_Y	0.911	1.00
18_L	22_M	0.909	1.00
184_P	188_K	0.900	1.00
100_S	104_Q	0.899	1.00
126_V	129_I	0.898	1.00
188_K	192_T	0.884	0.99
177_E	180_Y	0.878	0.99
193_H	197_L	0.876	0.99
93_E	97_M	0.876	0.99
125_I	167_Y	0.875	0.99
189_F	206_L	0.874	0.99
20_L	167_Y	0.870	0.99
71_G	97_M	0.868	0.99
204_T	207_A	0.860	0.99
122_I	168_L	0.860	0.99
75_R	78_Q	0.858	0.99
148_V	155_E	0.856	0.99
217_V	222_K	0.850	0.99
141_Y	165_N	0.847	0.99
55_S	58_D	0.844	0.99
162_K	169_R	0.840	0.99
47_S	50_R	0.837	0.99
88_R	91_L	0.831	0.99
15_L	94_V	0.830	0.99
102_V	105_D	0.827	0.99
44_E	47_S	0.827	0.99
30_E	84_L	0.827	0.99
102_V	120_G	0.820	0.99
121_I	125_I	0.812	0.99
134_R	215_W	0.807	0.99
114_I	210_L	0.806	0.99
145_D	161_S	0.802	0.99
89_E	93_E	0.801	0.99
50_R	58_D	0.799	0.99
111_I	114_I	0.798	0.99
29_Q	80_L	0.794	0.99
23_K	27_T	0.793	0.99
145_D	148_V	0.793	0.99
192_T	195_D	0.791	0.99
53_K	56_K	0.791	0.99
99_E	103_P	0.789	0.99
50_R	54_F	0.789	0.99
28_K	31_N	0.785	0.99
75_R	79_V	0.775	0.99
193_H	205_A	0.768	0.98
177_E	203_Y	0.762	0.98
16_V	98_I	0.760	0.98
129_I	215_W	0.758	0.98
55_S	61_D	0.755	0.98
198_K	202_L	0.748	0.98
44_E	54_F	0.741	0.98
19_Y	117_L	0.737	0.98
51_L	57_G	0.734	0.98
29_Q	32_R	0.733	0.98
140_A	145_D	0.732	0.98
79_V	83_I	0.732	0.98
29_Q	83_I	0.727	0.98
147_V	161_S	0.726	0.98
180_Y	194_K	0.724	0.98
185_T	224_Y	0.722	0.98
74_L	78_Q	0.719	0.98
72_T	75_R	0.714	0.98
160_I	205_A	0.714	0.98
144_L	208_R	0.714	0.98
29_Q	84_L	0.711	0.98
13_R	125_I	0.706	0.97
19_Y	91_L	0.702	0.97
16_V	120_G	0.700	0.97
73_V	76_E	0.700	0.97
21_F	25_A	0.699	0.97
136_E	208_R	0.698	0.97
51_L	55_S	0.697	0.97
10_N	14_E	0.694	0.97
114_I	122_I	0.694	0.97
145_D	162_K	0.686	0.97
49_E	53_K	0.679	0.97
83_I	87_T	0.679	0.97
178_T	182_R	0.673	0.97
22_M	90_E	0.672	0.97
180_Y	203_Y	0.668	0.96
212_R	215_W	0.663	0.96
143_G	169_R	0.661	0.96
14_E	36_A	0.660	0.96
48_E	51_L	0.659	0.96
177_E	207_A	0.658	0.96
109_L	119_A	0.658	0.96
164_G	169_R	0.657	0.96
172_F	207_A	0.652	0.96
52_E	56_K	0.650	0.96
21_F	24_D	0.649	0.96
91_L	95_G	0.649	0.96
188_K	191_E	0.648	0.96
80_L	83_I	0.646	0.96
190_Y	194_K	0.645	0.96
85_L	89_E	0.645	0.96
53_K	57_G	0.643	0.96
105_D	109_L	0.642	0.95
128_D	131_R	0.637	0.95
169_R	204_T	0.629	0.95
158_K	161_S	0.628	0.95
214_V	217_V	0.626	0.95
121_I	167_Y	0.626	0.95
18_L	21_F	0.614	0.94
139_V	212_R	0.613	0.94
135_P	212_R	0.612	0.94
107_V	221_N	0.612	0.94
51_L	59_F	0.609	0.94
46_L	54_F	0.609	0.94
31_N	35_R	0.608	0.94
22_M	94_V	0.608	0.94
8_T	104_Q	0.605	0.94
183_N	224_Y	0.601	0.94
38_F	43_E	0.596	0.93
148_V	157_R	0.596	0.93
46_L	58_D	0.595	0.93
9_Y	12_L	0.595	0.93
30_E	33_L	0.595	0.93
14_E	76_E	0.592	0.93
145_D	163_R	0.589	0.93
18_L	76_E	0.589	0.93
35_R	41_N	0.589	0.93
89_E	92_K	0.588	0.93
21_F	28_K	0.584	0.93
61_D	67_L	0.582	0.93
76_E	79_V	0.581	0.92
15_L	97_M	0.581	0.92
108_L	114_I	0.575	0.92
179_Q	186_L	0.572	0.92
7_D	11_P	0.570	0.92
194_K	197_L	0.570	0.92
95_G	117_L	0.565	0.91
16_V	121_I	0.564	0.91
98_I	120_G	0.564	0.91
135_P	215_W	0.563	0.91
126_V	142_C	0.559	0.91
47_S	58_D	0.558	0.91
49_E	60_S	0.557	0.91
99_E	109_L	0.555	0.91
70_T	73_V	0.553	0.91
13_R	16_V	0.553	0.91
204_T	217_V	0.552	0.90
45_K	50_R	0.552	0.90
120_G	167_Y	0.550	0.90
72_T	76_E	0.548	0.90
34_R	38_F	0.543	0.90
209_K	212_R	0.543	0.90
46_L	51_L	0.541	0.90
185_T	213_I	0.540	0.89
174_F	178_T	0.539	0.89
54_F	60_S	0.539	0.89
175_L	207_A	0.538	0.89
10_N	13_R	0.538	0.89
150_R	154_I	0.536	0.89
177_E	181_S	0.532	0.89
107_V	111_I	0.532	0.89
139_V	148_V	0.531	0.89
78_Q	82_R	0.530	0.89
44_E	56_K	0.529	0.89
180_Y	206_L	0.529	0.89
213_I	216_S	0.528	0.88
130_R	219_Y	0.527	0.88
144_L	169_R	0.525	0.88
81_A	131_R	0.523	0.88
93_E	96_K	0.522	0.88
170_S	173_Y	0.519	0.88
18_L	32_R	0.518	0.87
138_F	214_V	0.518	0.87
117_L	121_I	0.516	0.87
53_K	59_F	0.514	0.87
95_G	116_K	0.514	0.87
52_E	59_F	0.513	0.87
87_T	90_E	0.513	0.87
201_K	205_A	0.512	0.87
18_L	80_L	0.509	0.87
32_R	80_L	0.509	0.87
183_N	186_L	0.509	0.87
126_V	141_Y	0.507	0.86
105_D	123_I	0.505	0.86
127_G	131_R	0.504	0.86
29_Q	37_L	0.502	0.86
110_T	123_I	0.500	0.86

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2nrtA	1	0.6476	87.7	0.913	Contact Map
1keaA	2	0.7974	87.3	0.914	Contact Map
2a1jB	2	0.3392	84.6	0.917	Contact Map
3c65A	1	0.3568	84.1	0.917	Contact Map
3icxA	2	0.7665	84	0.918	Contact Map
4glxA	1	0.6035	83.3	0.918	Contact Map
3c1yA	5	0.7225	81.1	0.92	Contact Map
3plaA	2	0.8899	80.1	0.921	Contact Map
2duyA	1	0.2863	79.2	0.922	Contact Map
2a1jA	1	0.2599	79.2	0.922	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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