Q97Z61 - SULSO - GREMLIN Contacts

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Q97Z61 - Second ORF in transposon ISC1904

UniProt:	Q97Z61	Sequences: MLKNLRIRKFEPEEEYVHFTYSIKNSEREKSKELIKEYRTLLQKAIDYLWNLTKIQVRKKNGNYKITLPKKKEVYKPLREELEKINHLASHYVDKAINDAFSILKSWRKRAIKGRASIEKPRVKKAYVRIKTTLRKVVGESVRITVRPHEYITFPWSKSWFSRRVRELELGEPIIKEEKVYLPFRYKLPWVTPVNFLAIDSNLYTLDAYDGEKFVTISLKQLYSLKYSMEVKRAKVQSFASKHTKRGRELLRKYSHRERNRVLDFVHKFVNTLLDLYPMTFFAVEKLNKESMFKDANGSLSRKISRTVWRSIHRVLKYKAPLYGSFVKEVNPHLTSRSCPRCGFVSRKVGKTFECERCGFKLDRQLNASLNIYLKMCGFPHIREIARVWVGVIPLMGRRGMNVRDFGEAQGLRVDIKYHEIL	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	422 (344)
Sequences:	4394 (2821)
Seq/√Len:	152.1

ECOLI - INSQ
HALSA - Q9HSW9 Q9HHJ9 Q9HM87 Q9HSW7 Q9HHG8 Q9HSZ6 Q9HSD4 Q9HHU6 Q9HSY4
SULSO - Q97Z61 Q97UQ8 Q97XQ0 Q97YW9 Q97U68 Q97U51 Q97YU1 Q97XB6 Q97YI5 Q97Y48 Q97YI2 Q97V25 Q97XQ8 Q97V47 Q97WZ3 Q97XP1 Q97Y44 Q97Y67 Q97X39 Q97YW4 Q7LXH2 Q97V53 Q97XA4 Q7LXD9 Q97WY6 Q7LXH6 Y2105 Q97YF7 Q7LX59 Q97U09

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
264_D	268_K	5.408	1.00
288_N	291_S	4.389	1.00
340_P	367_N	4.114	1.00
320_A	327_V	3.625	1.00
206_L	312_I	3.056	1.00
284_V	316_L	2.988	1.00
313_H	329_E	2.963	1.00
19_F	322_L	2.936	1.00
347_R	350_G	2.864	1.00
271_N	275_D	2.785	1.00
311_S	315_V	2.776	1.00
283_A	376_M	2.653	1.00
312_I	316_L	2.443	1.00
173_P	182_L	2.418	1.00
338_S	345_V	2.418	1.00
337_R	346_S	2.362	1.00
334_L	337_R	2.362	1.00
330_V	372_I	2.329	1.00
311_S	314_R	2.329	1.00
317_K	329_E	2.319	1.00
274_L	320_A	2.308	1.00
226_K	302_R	2.222	1.00
293_F	305_S	2.214	1.00
282_F	320_A	2.177	1.00
225_L	261_R	2.129	1.00
171_G	187_K	2.128	1.00
267_H	315_V	2.099	1.00
232_K	247_G	2.065	1.00
335_T	372_I	2.051	1.00
284_V	327_V	2.048	1.00
267_H	318_Y	2.025	1.00
354_E	361_K	2.015	1.00
51_N	55_I	1.995	1.00
28_R	31_S	1.975	1.00
54_K	120_K	1.974	1.00
206_L	265_F	1.968	1.00
298_G	301_S	1.955	1.00
49_L	97_I	1.917	1.00
36_K	180_V	1.911	1.00
342_C	358_C	1.896	1.00
263_L	311_S	1.873	1.00
270_V	315_V	1.864	1.00
351_K	364_R	1.818	1.00
317_K	321_P	1.818	1.00
266_V	315_V	1.809	1.00
267_H	319_K	1.808	1.00
37_E	87_H	1.807	1.00
247_G	251_L	1.806	1.00
341_R	360_F	1.799	1.00
50_W	100_A	1.785	1.00
342_C	355_C	1.763	1.00
353_F	362_L	1.750	1.00
355_C	358_C	1.743	1.00
339_C	358_C	1.733	1.00
267_H	270_V	1.711	1.00
339_C	342_C	1.711	1.00
242_K	245_K	1.685	1.00
284_V	329_E	1.661	1.00
44_K	84_K	1.640	1.00
232_K	235_K	1.622	1.00
266_V	311_S	1.607	1.00
245_K	248_R	1.606	1.00
346_S	350_G	1.603	1.00
199_I	207_D	1.602	1.00
339_C	355_C	1.591	1.00
177_E	256_H	1.545	1.00
35_I	180_V	1.534	1.00
20_T	183_P	1.530	1.00
68_L	72_K	1.525	1.00
33_E	37_E	1.524	1.00
278_P	325_S	1.521	1.00
286_K	329_E	1.510	1.00
196_F	373_Y	1.510	1.00
284_V	313_H	1.493	1.00
208_A	366_L	1.491	1.00
270_V	318_Y	1.482	1.00
352_T	361_K	1.458	1.00
32_K	36_K	1.451	1.00
353_F	360_F	1.449	1.00
101_F	105_K	1.445	1.00
344_F	356_E	1.437	1.00
179_K	256_H	1.431	1.00
236_V	248_R	1.420	1.00
205_T	208_A	1.409	1.00
39_R	180_V	1.396	1.00
370_L	374_L	1.380	1.00
106_S	118_I	1.378	1.00
49_L	100_A	1.369	1.00
285_E	332_P	1.366	1.00
348_K	351_K	1.365	1.00
317_K	327_V	1.360	1.00
69_P	72_K	1.357	1.00
256_H	260_N	1.356	1.00
209_Y	213_K	1.350	1.00
54_K	58_R	1.344	1.00
110_R	118_I	1.344	1.00
270_V	319_K	1.336	1.00
295_D	301_S	1.335	1.00
145_T	151_Y	1.323	1.00
104_L	120_K	1.318	1.00
91_H	131_K	1.310	1.00
197_L	209_Y	1.306	1.00
111_A	118_I	1.302	1.00
233_R	237_Q	1.257	1.00
232_K	254_Y	1.250	1.00
24_K	179_K	1.236	1.00
105_K	109_K	1.234	1.00
304_I	309_W	1.232	1.00
196_F	281_F	1.222	1.00
29_E	32_K	1.214	1.00
42_L	96_A	1.192	1.00
366_L	370_L	1.191	1.00
70_K	73_E	1.190	1.00
288_N	332_P	1.189	1.00
102_S	106_S	1.187	1.00
229_M	255_S	1.186	1.00
272_T	276_L	1.183	1.00
95_K	98_N	1.182	1.00
27_E	31_S	1.180	1.00
144_I	182_L	1.176	1.00
347_R	351_K	1.168	1.00
197_L	277_Y	1.163	1.00
228_S	232_K	1.151	1.00
270_V	312_I	1.151	1.00
330_V	375_K	1.150	1.00
201_S	309_W	1.141	1.00
79_R	93_V	1.139	1.00
229_M	251_L	1.139	1.00
62_G	65_K	1.133	1.00
340_P	370_L	1.130	1.00
266_V	312_I	1.125	1.00
315_V	318_Y	1.115	0.99
198_A	373_Y	1.115	0.99
139_G	142_V	1.113	0.99
335_T	375_K	1.111	0.99
274_L	323_Y	1.104	0.99
225_L	258_E	1.101	0.99
146_V	175_I	1.096	0.99
263_L	315_V	1.096	0.99
338_S	375_K	1.094	0.99
271_N	323_Y	1.084	0.99
91_H	133_T	1.082	0.99
203_L	307_T	1.072	0.99
46_I	96_A	1.066	0.99
240_A	244_T	1.064	0.99
266_V	270_V	1.061	0.99
201_S	313_H	1.061	0.99
106_S	111_A	1.058	0.99
274_L	282_F	1.057	0.99
270_V	316_L	1.056	0.99
283_A	372_I	1.051	0.99
110_R	119_E	1.051	0.99
99_D	129_R	1.048	0.99
275_D	323_Y	1.046	0.99
348_K	364_R	1.045	0.99
362_L	370_L	1.043	0.99
244_T	248_R	1.031	0.99
89_A	92_Y	1.030	0.99
27_E	35_I	1.029	0.99
259_R	263_L	1.024	0.99
347_R	364_R	1.022	0.99
362_L	366_L	1.020	0.99
128_V	131_K	1.018	0.99
230_E	233_R	1.013	0.99
199_I	209_Y	1.013	0.99
236_V	251_L	1.013	0.99
214_F	363_D	1.009	0.99
77_P	81_E	1.006	0.99
340_P	362_L	1.005	0.99
21_Y	186_Y	1.004	0.99
295_D	299_S	1.004	0.99
73_E	76_K	1.004	0.99
233_R	251_L	0.994	0.99
286_K	332_P	0.993	0.99
41_L	84_K	0.980	0.99
107_W	120_K	0.980	0.99
274_L	325_S	0.976	0.99
197_L	210_D	0.975	0.99
232_K	250_L	0.975	0.99
106_S	110_R	0.973	0.99
314_R	317_K	0.973	0.99
50_W	104_L	0.972	0.99
32_K	180_V	0.971	0.99
319_K	322_L	0.966	0.99
136_K	145_T	0.966	0.99
128_V	132_T	0.958	0.98
289_K	310_R	0.958	0.98
351_K	363_D	0.957	0.98
232_K	251_L	0.951	0.98
346_S	353_F	0.951	0.98
229_M	258_E	0.948	0.98
107_W	121_P	0.947	0.98
71_K	75_Y	0.946	0.98
66_I	70_K	0.945	0.98
295_D	298_G	0.943	0.98
213_K	272_T	0.940	0.98
332_P	335_T	0.939	0.98
337_R	345_V	0.937	0.98
334_L	375_K	0.933	0.98
344_F	357_R	0.931	0.98
129_R	172_E	0.929	0.98
281_F	326_F	0.928	0.98
55_I	59_K	0.927	0.98
138_V	141_S	0.927	0.98
40_T	43_Q	0.925	0.98
46_I	100_A	0.922	0.98
352_T	362_L	0.917	0.98
193_P	278_P	0.912	0.98
229_M	237_Q	0.905	0.98
91_H	95_K	0.901	0.98
221_Q	225_L	0.901	0.98
196_F	283_A	0.900	0.98
44_K	48_Y	0.898	0.98
218_S	307_T	0.895	0.98
53_T	101_F	0.895	0.98
266_V	308_V	0.893	0.98
156_W	159_S	0.893	0.98
54_K	104_L	0.891	0.97
70_K	75_Y	0.891	0.97
18_H	187_K	0.887	0.97
208_A	214_F	0.883	0.97
203_L	303_K	0.882	0.97
106_S	109_K	0.878	0.97
207_D	269_F	0.878	0.97
340_P	360_F	0.876	0.97
205_T	216_T	0.875	0.97
142_V	145_T	0.874	0.97
236_V	247_G	0.872	0.97
143_R	151_Y	0.870	0.97
134_L	147_R	0.867	0.97
76_K	79_R	0.867	0.97
95_K	129_R	0.866	0.97
233_R	255_S	0.865	0.97
366_L	369_S	0.863	0.97
71_K	74_V	0.862	0.97
320_A	325_S	0.861	0.97
46_I	103_I	0.852	0.97
66_I	69_P	0.852	0.97
136_K	139_G	0.852	0.97
250_L	253_K	0.850	0.97
341_R	370_L	0.849	0.97
281_F	328_K	0.848	0.97
134_L	137_V	0.847	0.97
18_H	185_R	0.846	0.97
205_T	214_F	0.845	0.97
368_A	371_N	0.843	0.96
350_G	364_R	0.843	0.96
76_K	80_E	0.841	0.96
69_P	75_Y	0.837	0.96
85_I	93_V	0.834	0.96
291_S	304_I	0.834	0.96
221_Q	261_R	0.830	0.96
330_V	376_M	0.827	0.96
69_P	73_E	0.825	0.96
273_L	282_F	0.825	0.96
94_D	98_N	0.819	0.96
346_S	364_R	0.818	0.96
340_P	374_L	0.818	0.96
271_N	319_K	0.818	0.96
99_D	174_I	0.816	0.96
230_E	234_A	0.812	0.96
338_S	371_N	0.812	0.96
293_F	301_S	0.809	0.96
201_S	207_D	0.807	0.95
72_K	76_K	0.806	0.95
25_N	182_L	0.805	0.95
257_R	261_R	0.803	0.95
340_P	368_A	0.803	0.95
215_V	265_F	0.802	0.95
174_I	183_P	0.800	0.95
175_I	182_L	0.800	0.95
248_R	252_R	0.794	0.95
289_K	309_W	0.794	0.95
291_S	332_P	0.793	0.95
67_T	71_K	0.788	0.95
48_Y	52_L	0.787	0.95
370_L	373_Y	0.784	0.95
229_M	233_R	0.784	0.95
269_F	272_T	0.780	0.95
367_N	371_N	0.778	0.94
149_H	152_I	0.778	0.94
97_I	101_F	0.777	0.94
45_A	96_A	0.775	0.94
91_H	132_T	0.774	0.94
154_F	157_S	0.769	0.94
286_K	313_H	0.769	0.94
254_Y	258_E	0.767	0.94
57_V	61_N	0.766	0.94
231_V	235_K	0.765	0.94
75_Y	79_R	0.762	0.94
265_F	269_F	0.762	0.94
195_N	211_G	0.761	0.94
95_K	99_D	0.759	0.94
353_F	364_R	0.757	0.94
197_L	282_F	0.755	0.94
200_D	365_Q	0.754	0.94
146_V	149_H	0.744	0.93
75_Y	94_D	0.740	0.93
66_I	71_K	0.739	0.93
144_I	173_P	0.739	0.93
214_F	366_L	0.737	0.93
192_T	195_N	0.736	0.93
24_K	181_Y	0.735	0.93
53_T	104_L	0.735	0.93
230_E	237_Q	0.734	0.93
95_K	131_K	0.733	0.93
52_L	74_V	0.732	0.93
75_Y	78_L	0.731	0.92
300_L	304_I	0.731	0.92
241_S	244_T	0.727	0.92
23_I	182_L	0.726	0.92
65_K	70_K	0.725	0.92
26_S	155_P	0.724	0.92
48_Y	78_L	0.724	0.92
344_F	353_F	0.722	0.92
346_S	351_K	0.721	0.92
350_G	353_F	0.720	0.92
38_Y	42_L	0.719	0.92
258_E	306_R	0.718	0.92
283_A	373_Y	0.718	0.92
169_E	187_K	0.717	0.92
218_S	221_Q	0.716	0.92
34_L	38_Y	0.715	0.92
204_Y	208_A	0.713	0.92
67_T	70_K	0.712	0.91
41_L	88_L	0.712	0.91
334_L	338_S	0.709	0.91
225_L	257_R	0.709	0.91
61_N	64_Y	0.709	0.91
91_H	94_D	0.708	0.91
133_T	136_K	0.708	0.91
100_A	104_L	0.703	0.91
206_L	270_V	0.700	0.91
52_L	78_L	0.699	0.91
40_T	44_K	0.699	0.91
91_H	134_L	0.698	0.91
29_E	33_E	0.698	0.91
172_E	185_R	0.695	0.90
68_L	71_K	0.693	0.90
37_E	41_L	0.692	0.90
104_L	108_R	0.692	0.90
287_L	332_P	0.692	0.90
74_V	97_I	0.686	0.90
88_L	91_H	0.685	0.90
273_L	277_Y	0.684	0.90
129_R	174_I	0.684	0.90
30_K	33_E	0.681	0.90
57_V	104_L	0.678	0.89
237_Q	251_L	0.677	0.89
202_N	208_A	0.675	0.89
313_H	317_K	0.673	0.89
310_R	314_R	0.673	0.89
190_W	193_P	0.672	0.89
236_V	244_T	0.672	0.89
190_W	325_S	0.671	0.89
262_V	306_R	0.671	0.89
298_G	305_S	0.669	0.89
145_T	148_P	0.668	0.89
283_A	375_K	0.667	0.89
206_L	307_T	0.664	0.88
41_L	44_K	0.664	0.88
334_L	345_V	0.664	0.88
58_R	61_N	0.663	0.88
235_K	247_G	0.663	0.88
275_D	281_F	0.662	0.88
209_Y	215_V	0.662	0.88
356_E	364_R	0.661	0.88
295_D	304_I	0.659	0.88
18_H	171_G	0.659	0.88
135_R	170_L	0.659	0.88
225_L	229_M	0.658	0.88
263_L	267_H	0.650	0.87
20_T	185_R	0.648	0.87
52_L	56_Q	0.645	0.87
107_W	110_R	0.645	0.87
316_L	327_V	0.644	0.87
35_I	39_R	0.639	0.86
207_D	312_I	0.638	0.86
282_F	316_L	0.637	0.86
320_A	323_Y	0.635	0.86
22_S	183_P	0.630	0.86
222_L	225_L	0.630	0.86
142_V	146_V	0.629	0.86
225_L	254_Y	0.629	0.86
72_K	75_Y	0.628	0.85
341_R	362_L	0.627	0.85
283_A	328_K	0.625	0.85
338_S	343_G	0.624	0.85
52_L	55_I	0.623	0.85
38_Y	89_A	0.623	0.85
154_F	158_K	0.622	0.85
23_I	165_V	0.618	0.85
92_Y	130_I	0.617	0.84
372_I	375_K	0.617	0.84
76_K	81_E	0.617	0.84
289_K	292_M	0.617	0.84
234_A	238_S	0.616	0.84
233_R	238_S	0.616	0.84
103_I	121_P	0.615	0.84
243_H	246_R	0.613	0.84
130_I	133_T	0.612	0.84
375_K	378_G	0.611	0.84
72_K	97_I	0.611	0.84
44_K	82_L	0.611	0.84
200_D	336_S	0.609	0.84
362_L	367_N	0.607	0.83
255_S	259_R	0.604	0.83
104_L	107_W	0.604	0.83
315_V	319_K	0.603	0.83
41_L	85_I	0.601	0.83
96_A	100_A	0.601	0.83
255_S	262_V	0.599	0.83
129_R	132_T	0.597	0.82
76_K	97_I	0.597	0.82
318_Y	322_L	0.594	0.82
232_K	236_V	0.593	0.82
350_G	356_E	0.591	0.82
71_K	98_N	0.590	0.82
17_V	187_K	0.589	0.82
70_K	74_V	0.589	0.82
97_I	100_A	0.589	0.82
328_K	376_M	0.587	0.81
39_R	128_V	0.587	0.81
195_N	277_Y	0.587	0.81
338_S	353_F	0.587	0.81
93_V	96_A	0.586	0.81
109_K	118_I	0.585	0.81
215_V	272_T	0.584	0.81
66_I	75_Y	0.584	0.81
222_L	226_K	0.582	0.81
260_N	263_L	0.581	0.81
23_I	158_K	0.579	0.81
51_N	123_V	0.579	0.81
204_Y	351_K	0.578	0.80
206_L	266_V	0.578	0.80
331_N	334_L	0.577	0.80
245_K	249_E	0.576	0.80
74_V	78_L	0.576	0.80
127_Y	131_K	0.575	0.80
244_T	251_L	0.574	0.80
38_Y	88_L	0.571	0.80
88_L	93_V	0.570	0.79
22_S	263_L	0.570	0.79
223_Y	302_R	0.567	0.79
179_K	260_N	0.565	0.79
269_F	312_I	0.564	0.79
34_L	37_E	0.564	0.79
96_A	99_D	0.563	0.79
333_H	337_R	0.563	0.79
235_K	238_S	0.563	0.79
27_E	32_K	0.562	0.79
90_S	133_T	0.561	0.78
203_L	294_K	0.560	0.78
268_K	271_N	0.559	0.78
293_F	298_G	0.559	0.78
208_A	216_T	0.558	0.78
244_T	247_G	0.557	0.78
223_Y	227_Y	0.557	0.78
203_L	293_F	0.556	0.78
37_E	40_T	0.553	0.77
38_Y	130_I	0.550	0.77
348_K	352_T	0.547	0.77
131_K	135_R	0.546	0.77
123_V	126_A	0.546	0.77
181_Y	256_H	0.546	0.77
291_S	294_K	0.545	0.76
51_N	54_K	0.543	0.76
310_R	313_H	0.542	0.76
289_K	304_I	0.541	0.76
165_V	186_Y	0.540	0.76
48_Y	84_K	0.539	0.76
210_D	366_L	0.539	0.76
130_I	134_L	0.539	0.76
127_Y	130_I	0.538	0.75
363_D	368_A	0.538	0.75
266_V	269_F	0.538	0.75
223_Y	303_K	0.537	0.75
354_E	360_F	0.536	0.75
201_S	284_V	0.536	0.75
277_Y	282_F	0.535	0.75
92_Y	128_V	0.534	0.75
291_S	295_D	0.534	0.75
77_P	80_E	0.533	0.75
301_S	305_S	0.530	0.74
176_K	179_K	0.530	0.74
198_A	283_A	0.529	0.74
135_R	139_G	0.529	0.74
237_Q	247_G	0.528	0.74
334_L	353_F	0.526	0.74
300_L	303_K	0.526	0.74
143_R	146_V	0.526	0.74
135_R	173_P	0.524	0.74
348_K	363_D	0.522	0.73
130_I	146_V	0.522	0.73
234_A	237_Q	0.522	0.73
131_K	134_L	0.522	0.73
21_Y	158_K	0.520	0.73
204_Y	347_R	0.519	0.73
68_L	98_N	0.519	0.73
90_S	134_L	0.518	0.73
39_R	42_L	0.517	0.73
230_E	298_G	0.517	0.73
230_E	238_S	0.515	0.72
223_Y	226_K	0.510	0.72
22_S	181_Y	0.510	0.72
295_D	305_S	0.508	0.71
91_H	97_I	0.508	0.71
181_Y	260_N	0.508	0.71
90_S	131_K	0.505	0.71
46_I	218_S	0.505	0.71
235_K	250_L	0.504	0.71
367_N	370_L	0.503	0.71
67_T	98_N	0.503	0.71
42_L	128_V	0.503	0.71
46_I	50_W	0.500	0.70
45_A	93_V	0.500	0.70

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1nu0A	1	0.3081	95.2	0.935	Contact Map
1vhxA	2	0.3246	94.9	0.936	Contact Map
1iv0A	1	0.2299	93.8	0.939	Contact Map
1qypA	1	0.1019	83	0.951	Contact Map
4aybP	1	0.0948	81.4	0.952	Contact Map
2k4xA	1	0.1256	79.1	0.953	Contact Map
3j20Y	1	0.1137	78.7	0.953	Contact Map
1ffkW	1	0.1564	75	0.955	Contact Map
3j3w0	1	0.1303	73.3	0.955	Contact Map
1vd4A	1	0.1256	69.4	0.956	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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