Q97Z08 - SULSO - GREMLIN Contacts

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Q97Z08 - Na(+)/H(+) antiporter

UniProt:	Q97Z08	Sequences: MNPIILALFDVSIFILLAEILSSLVQKYGLPKLIGELLAGMIIGPYALGSLLNQLLGFPLIGINSYIEFLAEFSVILLIFASGLEHGIAPIKSSGILGFLGATFGALLPFFAAYYFYISSFGLDSALILGTAMGATSLAAVASIIEEGKLKGKGINFMVSSAAADDVVDLLLLSVVIAILQGESTSVTSISLKIIALVVIWVVILVVSIILIPKITDRISDKYIEEFPLAVLFGLTLLMVSLGYSPIISAFVAGVAFANSVKNSKIKEISSTLLSVFGPLFFVYIGAEVNFLTLNLNTVILSLELTAIATIFKWLGIFPFALAYLRNAKAANTVALGMVPRGETGLVIASLGLSYNALNQEEFEGIVFMSIFTTLIGSALFKIYAKKHLVS	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	391 (365)
Sequences:	15143 (10387)
Seq/√Len:	543.7

ECOLI - NHAP2 YBAL KEFB KEFC YJCE
BACSU - YJBQ YHAU NHAK
HALSA - Q9HNK1
SULSO - Q97Z08 Q97UN8 Q980D8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
214_K	218_R	3.677	1.00
318_F	328_A	3.625	1.00
112_A	312_F	3.616	1.00
206_V	210_I	3.384	1.00
192_L	196_A	3.345	1.00
306_T	372_F	3.329	1.00
210_I	214_K	3.296	1.00
336_L	380_L	3.273	1.00
122_G	125_S	2.906	1.00
214_K	217_D	2.856	1.00
327_N	330_A	2.803	1.00
114_Y	130_G	2.615	1.00
208_S	235_L	2.414	1.00
235_L	253_A	2.411	1.00
196_A	200_I	2.389	1.00
303_L	307_A	2.382	1.00
200_I	244_Y	2.365	1.00
116_F	308_I	2.365	1.00
92_K	95_G	2.349	1.00
193_K	196_A	2.339	1.00
302_S	368_F	2.331	1.00
114_Y	123_L	2.250	1.00
188_T	192_L	2.170	1.00
114_Y	117_Y	2.120	1.00
111_F	115_Y	2.081	1.00
115_Y	123_L	2.023	1.00
299_V	303_L	2.014	1.00
345_G	366_I	1.994	1.00
195_I	199_V	1.986	1.00
98_G	160_S	1.943	1.00
350_S	353_L	1.936	1.00
300_I	304_E	1.927	1.00
318_F	335_A	1.909	1.00
350_S	354_S	1.903	1.00
65_S	68_E	1.883	1.00
322_L	331_A	1.874	1.00
191_S	195_I	1.854	1.00
207_V	210_I	1.839	1.00
128_I	352_G	1.831	1.00
353_L	363_F	1.813	1.00
80_F	232_L	1.805	1.00
21_L	39_A	1.778	1.00
87_G	90_P	1.773	1.00
124_D	176_V	1.757	1.00
45_P	49_G	1.753	1.00
236_T	246_P	1.742	1.00
305_L	369_M	1.730	1.00
319_P	323_A	1.730	1.00
321_A	331_A	1.720	1.00
187_V	190_I	1.718	1.00
231_V	253_A	1.714	1.00
314_W	335_A	1.710	1.00
42_I	47_A	1.696	1.00
154_G	334_V	1.694	1.00
117_Y	123_L	1.692	1.00
322_L	328_A	1.684	1.00
234_G	238_L	1.679	1.00
316_G	320_F	1.675	1.00
89_A	92_K	1.668	1.00
295_N	299_V	1.663	1.00
76_I	236_T	1.654	1.00
372_F	376_I	1.647	1.00
25_V	35_G	1.641	1.00
349_A	366_I	1.640	1.00
205_L	209_I	1.639	1.00
170_L	348_I	1.597	1.00
359_N	362_E	1.573	1.00
199_V	202_V	1.562	1.00
126_A	130_G	1.555	1.00
38_L	42_I	1.539	1.00
66_Y	69_F	1.533	1.00
158_M	337_G	1.526	1.00
114_Y	127_L	1.525	1.00
199_V	203_V	1.520	1.00
189_S	192_L	1.516	1.00
239_M	249_S	1.511	1.00
212_I	231_V	1.499	1.00
112_A	115_Y	1.485	1.00
68_E	72_E	1.485	1.00
21_L	25_V	1.483	1.00
327_N	331_A	1.462	1.00
308_I	312_F	1.459	1.00
123_L	127_L	1.446	1.00
110_F	134_G	1.440	1.00
152_G	155_I	1.434	1.00
290_N	293_T	1.433	1.00
218_R	221_D	1.417	1.00
318_F	331_A	1.410	1.00
154_G	330_A	1.402	1.00
158_M	334_V	1.396	1.00
117_Y	126_A	1.391	1.00
100_L	324_Y	1.388	1.00
103_T	107_L	1.384	1.00
200_I	204_I	1.380	1.00
42_I	45_P	1.377	1.00
318_F	322_L	1.364	1.00
12_S	16_L	1.358	1.00
95_G	99_F	1.352	1.00
379_A	383_I	1.342	1.00
193_K	197_L	1.341	1.00
192_L	195_I	1.327	1.00
145_I	155_I	1.326	1.00
309_A	369_M	1.316	1.00
111_F	114_Y	1.315	1.00
14_F	283_V	1.313	1.00
318_F	332_N	1.309	1.00
89_A	93_S	1.304	1.00
209_I	213_P	1.303	1.00
46_Y	49_G	1.302	1.00
240_V	246_P	1.299	1.00
135_A	169_D	1.298	1.00
45_P	48_L	1.298	1.00
187_V	191_S	1.296	1.00
44_G	47_A	1.291	1.00
153_K	330_A	1.288	1.00
23_S	27_K	1.286	1.00
107_L	111_F	1.285	1.00
353_L	360_Q	1.277	1.00
346_L	370_S	1.272	1.00
17_L	39_A	1.270	1.00
211_L	214_K	1.269	1.00
132_A	349_A	1.268	1.00
154_G	333_T	1.267	1.00
149_K	154_G	1.264	1.00
208_S	212_I	1.261	1.00
105_G	109_P	1.255	1.00
12_S	230_A	1.254	1.00
351_L	354_S	1.253	1.00
103_T	106_A	1.244	1.00
133_M	369_M	1.243	1.00
237_L	241_S	1.240	1.00
80_F	254_G	1.239	1.00
312_F	316_G	1.237	1.00
97_L	157_F	1.225	1.00
23_S	26_Q	1.221	1.00
356_N	360_Q	1.204	1.00
310_T	339_V	1.202	1.00
96_I	100_L	1.200	1.00
189_S	193_K	1.199	1.00
16_L	230_A	1.197	1.00
201_W	205_L	1.194	1.00
90_P	93_S	1.188	1.00
188_T	191_S	1.187	1.00
109_P	113_A	1.179	1.00
302_S	306_T	1.178	1.00
25_V	28_Y	1.177	1.00
110_F	131_T	1.174	1.00
94_S	97_L	1.169	1.00
218_R	222_K	1.162	1.00
104_F	107_L	1.157	1.00
143_S	147_E	1.149	1.00
235_L	249_S	1.148	1.00
309_A	341_R	1.147	1.00
300_I	303_L	1.145	1.00
328_A	332_N	1.144	1.00
157_F	326_R	1.134	1.00
90_P	156_N	1.133	1.00
129_L	133_M	1.131	1.00
95_G	98_G	1.130	1.00
239_M	244_Y	1.130	1.00
202_V	206_V	1.130	1.00
130_G	134_G	1.124	1.00
198_V	202_V	1.123	1.00
203_V	206_V	1.122	1.00
301_L	365_G	1.119	1.00
20_I	23_S	1.117	1.00
315_L	319_P	1.113	1.00
326_R	330_A	1.109	1.00
352_G	355_Y	1.104	1.00
238_L	241_S	1.096	1.00
358_L	361_E	1.089	1.00
194_I	198_V	1.083	1.00
203_V	207_V	1.072	1.00
12_S	234_G	1.070	1.00
230_A	234_G	1.070	1.00
124_D	180_L	1.063	1.00
197_L	201_W	1.055	1.00
106_A	168_V	1.051	1.00
194_I	197_L	1.050	1.00
12_S	237_L	1.049	1.00
222_K	225_E	1.039	1.00
145_I	158_M	1.035	1.00
305_L	365_G	1.034	1.00
129_L	366_I	1.033	1.00
337_G	381_F	1.032	1.00
81_A	85_E	1.029	1.00
94_S	98_G	1.027	1.00
296_L	300_I	1.026	1.00
163_A	251_F	1.025	1.00
136_T	141_V	1.023	1.00
132_A	366_I	1.023	1.00
269_I	273_L	1.022	1.00
46_Y	290_N	1.018	1.00
108_L	111_F	1.017	1.00
351_L	355_Y	1.011	1.00
361_E	365_G	1.009	1.00
340_P	377_G	1.007	1.00
366_I	370_S	1.000	1.00
307_A	311_I	0.998	1.00
206_V	256_A	0.998	1.00
22_S	32_K	0.996	1.00
260_S	263_N	0.995	1.00
100_L	104_F	0.989	1.00
9_F	12_S	0.982	1.00
100_L	323_A	0.982	1.00
314_W	332_N	0.980	1.00
67_I	284_Y	0.966	1.00
222_K	226_E	0.962	1.00
329_K	333_T	0.959	1.00
121_F	129_L	0.959	1.00
196_A	199_V	0.958	1.00
332_N	335_A	0.957	1.00
228_P	232_L	0.957	1.00
234_G	237_L	0.956	1.00
341_R	345_G	0.956	1.00
133_M	309_A	0.951	1.00
145_I	150_L	0.951	1.00
19_E	22_S	0.945	1.00
88_I	92_K	0.943	1.00
110_F	172_L	0.940	1.00
365_G	369_M	0.930	1.00
310_T	314_W	0.929	1.00
79_I	247_I	0.925	1.00
306_T	310_T	0.922	1.00
135_A	345_G	0.918	1.00
19_E	230_A	0.915	1.00
136_T	162_A	0.911	1.00
63_I	66_Y	0.911	1.00
326_R	331_A	0.910	1.00
127_L	176_V	0.910	1.00
21_L	24_L	0.910	1.00
302_S	365_G	0.909	1.00
96_I	99_F	0.908	1.00
122_G	126_A	0.905	1.00
116_F	304_E	0.903	1.00
41_M	291_F	0.900	1.00
26_Q	29_G	0.900	1.00
110_F	114_Y	0.898	1.00
320_F	324_Y	0.896	1.00
195_I	198_V	0.894	1.00
309_A	373_T	0.891	1.00
24_L	27_K	0.890	1.00
131_T	172_L	0.887	1.00
322_L	325_L	0.881	1.00
104_F	320_F	0.880	1.00
108_L	316_G	0.880	1.00
299_V	302_S	0.878	1.00
301_L	304_E	0.874	1.00
204_I	244_Y	0.873	1.00
87_G	259_N	0.871	1.00
329_K	332_N	0.871	1.00
17_L	21_L	0.868	1.00
352_G	356_N	0.868	1.00
18_A	283_V	0.866	1.00
112_A	116_F	0.864	1.00
270_S	273_L	0.855	1.00
112_A	316_G	0.854	1.00
97_L	100_L	0.851	1.00
177_I	355_Y	0.848	1.00
128_I	180_L	0.847	1.00
311_I	315_L	0.845	1.00
303_L	306_T	0.844	1.00
35_G	39_A	0.839	1.00
250_A	254_G	0.838	1.00
228_P	231_V	0.837	1.00
321_A	326_R	0.834	1.00
41_M	47_A	0.833	1.00
204_I	235_L	0.833	1.00
285_I	367_V	0.831	1.00
131_T	348_I	0.830	1.00
22_S	35_G	0.829	1.00
125_S	359_N	0.829	1.00
293_T	364_E	0.827	1.00
319_P	322_L	0.823	1.00
84_L	257_F	0.821	1.00
36_E	279_P	0.821	1.00
357_A	361_E	0.821	1.00
229_L	232_L	0.819	1.00
331_A	335_A	0.819	1.00
380_L	383_I	0.817	1.00
80_F	228_P	0.817	1.00
46_Y	291_F	0.816	1.00
81_A	274_L	0.815	1.00
213_P	256_A	0.810	1.00
296_L	299_V	0.810	1.00
356_N	359_N	0.807	1.00
317_I	334_V	0.807	1.00
99_F	103_T	0.807	1.00
210_I	213_P	0.806	1.00
341_R	370_S	0.805	1.00
186_S	189_S	0.804	1.00
17_L	43_I	0.803	1.00
237_L	240_V	0.803	1.00
40_G	44_G	0.802	1.00
44_G	49_G	0.800	1.00
103_T	168_V	0.800	1.00
357_A	360_Q	0.799	1.00
137_S	165_D	0.798	1.00
145_I	149_K	0.793	1.00
37_L	371_I	0.791	1.00
232_L	236_T	0.791	1.00
304_E	307_A	0.784	1.00
43_I	287_A	0.777	1.00
22_S	26_Q	0.777	1.00
141_V	337_G	0.775	1.00
137_S	140_A	0.772	1.00
361_E	364_E	0.771	1.00
76_I	246_P	0.767	1.00
310_T	376_I	0.766	1.00
176_V	180_L	0.766	1.00
349_A	363_F	0.766	1.00
94_S	153_K	0.765	1.00
333_T	337_G	0.765	1.00
235_L	239_M	0.764	1.00
136_T	165_D	0.764	1.00
77_L	277_F	0.762	1.00
138_L	162_A	0.760	1.00
152_G	156_N	0.759	1.00
108_L	112_A	0.754	1.00
83_G	255_V	0.754	1.00
148_G	151_K	0.753	1.00
22_S	25_V	0.752	1.00
374_T	378_S	0.746	1.00
185_T	188_T	0.744	1.00
292_L	296_L	0.744	1.00
14_F	18_A	0.744	1.00
314_W	318_F	0.743	1.00
363_F	366_I	0.738	1.00
18_A	22_S	0.738	1.00
271_S	275_S	0.736	1.00
102_A	105_G	0.733	1.00
160_S	163_A	0.731	1.00
41_M	44_G	0.730	1.00
113_A	133_M	0.729	1.00
370_S	374_T	0.727	1.00
333_T	381_F	0.726	1.00
101_G	317_I	0.724	1.00
262_K	265_K	0.720	1.00
325_L	331_A	0.720	1.00
217_D	221_D	0.719	1.00
77_L	273_L	0.714	1.00
45_P	290_N	0.713	1.00
213_P	260_S	0.712	1.00
328_A	331_A	0.711	1.00
289_V	367_V	0.711	1.00
175_V	190_I	0.709	1.00
301_L	305_L	0.707	1.00
68_E	288_E	0.706	1.00
105_G	313_K	0.706	1.00
97_L	101_G	0.705	1.00
183_E	186_S	0.703	1.00
100_L	320_F	0.699	1.00
76_I	232_L	0.698	1.00
236_T	250_A	0.698	1.00
311_I	314_W	0.697	1.00
74_S	281_F	0.695	1.00
229_L	233_F	0.695	1.00
207_V	211_L	0.694	1.00
123_L	126_A	0.694	1.00
170_L	245_S	0.694	1.00
255_V	258_A	0.691	1.00
336_L	384_Y	0.690	1.00
12_S	19_E	0.690	1.00
281_F	343_E	0.690	1.00
97_L	324_Y	0.686	1.00
213_P	217_D	0.685	1.00
165_D	339_V	0.684	1.00
32_K	36_E	0.684	1.00
292_L	295_N	0.682	1.00
360_Q	364_E	0.682	1.00
20_I	24_L	0.681	1.00
212_I	216_T	0.681	1.00
182_G	186_S	0.673	1.00
41_M	46_Y	0.670	1.00
306_T	373_T	0.669	1.00
314_W	336_L	0.669	1.00
88_I	91_I	0.666	1.00
213_P	216_T	0.666	1.00
162_A	165_D	0.665	1.00
281_F	347_V	0.664	1.00
77_L	232_L	0.664	1.00
102_A	107_L	0.662	1.00
99_F	102_A	0.661	1.00
293_T	296_L	0.661	1.00
309_A	313_K	0.661	1.00
228_P	266_I	0.659	1.00
196_A	244_Y	0.655	1.00
330_A	333_T	0.653	1.00
236_T	240_V	0.652	1.00
349_A	362_E	0.651	1.00
107_L	110_F	0.649	1.00
185_T	190_I	0.647	1.00
13_I	17_L	0.646	1.00
124_D	179_I	0.645	1.00
232_L	235_L	0.642	1.00
212_I	253_A	0.641	1.00
228_P	270_S	0.641	1.00
85_E	146_E	0.640	1.00
375_L	379_A	0.639	1.00
216_T	226_E	0.639	1.00
9_F	13_I	0.634	1.00
121_F	125_S	0.632	1.00
186_S	190_I	0.631	1.00
129_L	365_G	0.629	1.00
228_P	257_F	0.629	1.00
376_I	380_L	0.627	1.00
97_L	326_R	0.627	1.00
49_G	60_L	0.627	1.00
102_A	161_S	0.627	1.00
198_V	201_W	0.626	1.00
285_I	343_E	0.626	1.00
117_Y	121_F	0.626	1.00
21_L	35_G	0.625	1.00
279_P	283_V	0.623	1.00
10_D	13_I	0.623	1.00
174_S	178_A	0.615	1.00
304_E	308_I	0.613	1.00
106_A	110_F	0.612	1.00
204_I	207_V	0.612	1.00
86_H	251_F	0.612	1.00
21_L	27_K	0.611	1.00
79_I	135_A	0.611	1.00
161_S	334_V	0.610	1.00
22_S	36_E	0.608	1.00
44_G	287_A	0.607	1.00
119_S	122_G	0.605	1.00
173_L	348_I	0.604	1.00
77_L	229_L	0.602	1.00
161_S	317_I	0.602	1.00
115_Y	118_I	0.602	1.00
88_I	255_V	0.602	1.00
72_E	240_V	0.601	1.00
344_T	347_V	0.597	1.00
185_T	189_S	0.596	1.00
135_A	138_L	0.589	1.00
204_I	239_M	0.588	1.00
97_L	153_K	0.587	1.00
67_I	71_A	0.585	0.99
332_N	384_Y	0.583	0.99
362_E	366_I	0.582	0.99
70_L	336_L	0.582	0.99
238_L	242_L	0.581	0.99
83_G	250_A	0.581	0.99
137_S	370_S	0.580	0.99
265_K	268_E	0.580	0.99
161_S	165_D	0.578	0.99
245_S	248_I	0.578	0.99
101_G	161_S	0.578	0.99
318_F	321_A	0.577	0.99
18_A	279_P	0.576	0.99
364_E	367_V	0.574	0.99
15_I	18_A	0.573	0.99
39_A	283_V	0.570	0.99
36_E	283_V	0.569	0.99
128_I	132_A	0.569	0.99
306_T	376_I	0.568	0.99
79_I	251_F	0.567	0.99
80_F	253_A	0.567	0.99
101_G	334_V	0.565	0.99
43_I	46_Y	0.565	0.99
200_I	242_L	0.565	0.99
290_N	360_Q	0.564	0.99
85_E	267_K	0.563	0.99
169_D	344_T	0.562	0.99
131_T	135_A	0.561	0.99
169_D	348_I	0.561	0.99
18_A	25_V	0.561	0.99
345_G	348_I	0.559	0.99
320_F	323_A	0.559	0.99
98_G	161_S	0.558	0.99
101_G	157_F	0.556	0.99
208_S	238_L	0.555	0.99
114_Y	126_A	0.554	0.99
140_A	374_T	0.553	0.99
359_N	363_F	0.553	0.99
136_T	342_G	0.553	0.99
44_G	48_L	0.553	0.99
34_I	375_L	0.551	0.99
89_A	259_N	0.550	0.99
321_A	334_V	0.550	0.99
256_A	259_N	0.549	0.99
62_G	65_S	0.549	0.99
113_A	130_G	0.547	0.99
24_L	28_Y	0.547	0.99
173_L	351_L	0.545	0.99
171_L	174_S	0.545	0.99
128_I	176_V	0.542	0.99
235_L	238_L	0.542	0.99
244_Y	249_S	0.542	0.99
252_V	256_A	0.541	0.99
210_I	256_A	0.540	0.99
263_N	267_K	0.539	0.99
19_E	227_F	0.538	0.99
261_V	264_S	0.537	0.99
375_L	378_S	0.536	0.99
219_I	225_E	0.535	0.99
314_W	317_I	0.534	0.99
141_V	144_I	0.534	0.99
190_I	194_I	0.533	0.99
377_G	380_L	0.532	0.99
336_L	377_G	0.532	0.99
133_M	341_R	0.532	0.99
204_I	252_V	0.531	0.99
137_S	345_G	0.531	0.99
135_A	165_D	0.531	0.99
221_D	224_I	0.530	0.99
229_L	273_L	0.530	0.99
191_S	194_I	0.530	0.99
220_S	225_E	0.529	0.99
360_Q	363_F	0.528	0.99
369_M	372_F	0.528	0.99
10_D	63_I	0.526	0.99
197_L	243_G	0.526	0.99
42_I	48_L	0.525	0.99
231_V	235_L	0.525	0.99
90_P	94_S	0.524	0.99
241_S	244_Y	0.524	0.99
170_L	344_T	0.523	0.99
131_T	134_G	0.523	0.99
177_I	180_L	0.522	0.99
105_G	165_D	0.521	0.99
268_E	271_S	0.520	0.99
125_S	129_L	0.520	0.99
197_L	200_I	0.519	0.99
217_D	261_V	0.519	0.99
101_G	105_G	0.517	0.99
211_L	215_I	0.516	0.99
142_A	159_V	0.516	0.99
189_S	196_A	0.515	0.99
110_F	113_A	0.514	0.99
221_D	226_E	0.514	0.99
201_W	209_I	0.513	0.99
202_V	248_I	0.512	0.99
230_A	237_L	0.512	0.99
144_I	147_E	0.512	0.99
22_S	279_P	0.512	0.99
374_T	377_G	0.511	0.99
15_I	22_S	0.511	0.99
71_A	75_V	0.510	0.99
315_L	318_F	0.508	0.99
369_M	373_T	0.508	0.99
178_A	182_G	0.506	0.99
306_T	309_A	0.506	0.99
334_V	337_G	0.505	0.99
118_I	122_G	0.505	0.99
79_I	169_D	0.504	0.99
101_G	321_A	0.503	0.98
289_V	371_I	0.500	0.98

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4bwzA	2	0.9591	100	0.219	Contact Map
4cz8A	2	0.9693	100	0.235	Contact Map
4czbA	2	0.9693	100	0.245	Contact Map
1zcdA	1	0.8491	99.9	0.598	Contact Map
2mdfA	1	0.1458	98.1	0.854	Contact Map
3zuxA	1	0.7647	97.7	0.869	Contact Map
4n7wA	2	0.7596	97.4	0.875	Contact Map
2k3cA	1	0.0793	88.9	0.919	Contact Map
2l0eA	1	0.0793	85.9	0.923	Contact Map
2m7xA	1	0.0767	71.2	0.933	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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