Q97YX0 - SULSO - GREMLIN Contacts

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Q97YX0 - First ORF in transposon ISC1904

UniProt:	Q97YX0	Sequences: MLRPKEACQRLGISYATLREYVKKGYIKPVILQSGKWRFREEDVERLMGIIRKRKVILYARVSSNTQKDDLVNQVKYLEEQVKEYDLVITDIGSKLNMKRKGFLKLLRMILNNEVSRVVVTYSDRLVRFGFEILEEVCKAHNCEIVVLNQEDKEEELVEDLVSILVSLSGKLYGMRSHEYEKVKKCAEELKNWKI	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	195 (183)
Sequences:	478 (332)
Seq/√Len:	24.5

SULSO - Q97YX0 Q97YU0 Q97YH2 Q97U52 Q97U67 Q97XB5 Q97UQ9 Q97YI6 Q97XI0 Q97XQ1 Q97XZ4 Q97X40 Q97Z60 Q97X25 Q97WZ6 Q97Y49

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
56_V	117_R	4.348	1.00
60_A	78_L	4.026	1.00
82_V	119_V	3.567	1.00
117_R	144_E	3.480	1.00
58_L	148_L	2.743	0.99
103_F	126_L	2.652	0.99
7_A	11_L	2.643	0.99
110_I	138_C	2.591	0.99
13_I	21_Y	2.438	0.98
17_T	21_Y	2.394	0.98
91_D	102_G	2.384	0.98
85_Y	146_V	2.359	0.98
135_E	139_K	2.310	0.98
5_K	9_Q	2.261	0.97
111_L	141_H	2.203	0.97
2_L	7_A	2.159	0.97
16_A	19_R	2.155	0.96
106_L	115_V	2.057	0.95
75_V	88_V	2.049	0.95
58_L	78_L	1.958	0.94
18_L	39_F	1.952	0.94
98_M	132_E	1.945	0.94
22_V	37_W	1.910	0.93
96_L	129_F	1.837	0.92
110_I	115_V	1.759	0.90
55_K	109_M	1.743	0.89
77_Y	121_T	1.742	0.89
119_V	132_E	1.721	0.89
75_V	90_T	1.638	0.86
110_I	118_V	1.622	0.86
131_F	145_I	1.602	0.85
3_R	34_S	1.595	0.85
58_L	88_V	1.592	0.84
89_I	102_G	1.574	0.84
138_C	143_C	1.534	0.82
72_V	76_K	1.531	0.82
98_M	167_S	1.528	0.82
127_V	131_F	1.526	0.82
109_M	114_E	1.512	0.81
4_P	19_R	1.505	0.81
22_V	29_P	1.501	0.81
27_I	30_V	1.461	0.79
26_Y	33_Q	1.460	0.79
40_R	43_D	1.448	0.78
166_V	177_S	1.442	0.78
16_A	24_K	1.425	0.77
131_F	147_V	1.423	0.77
12_G	142_N	1.400	0.76
56_V	144_E	1.372	0.74
122_Y	159_E	1.363	0.74
70_D	73_N	1.354	0.73
31_I	37_W	1.337	0.72
155_E	159_E	1.324	0.72
119_V	146_V	1.313	0.71
121_T	178_H	1.301	0.70
17_T	20_E	1.290	0.70
96_L	130_G	1.280	0.69
167_S	170_G	1.273	0.69
8_C	13_I	1.263	0.68
76_K	79_E	1.247	0.67
57_I	75_V	1.223	0.65
107_L	111_L	1.217	0.65
4_P	18_L	1.215	0.65
22_V	27_I	1.214	0.65
7_A	44_V	1.204	0.64
89_I	118_V	1.188	0.63
7_A	27_I	1.179	0.62
138_C	172_L	1.179	0.62
95_K	126_L	1.168	0.62
3_R	38_R	1.167	0.62
91_D	167_S	1.160	0.61
15_Y	19_R	1.134	0.59
78_L	148_L	1.134	0.59
14_S	20_E	1.132	0.59
2_L	38_R	1.117	0.58
170_G	175_M	1.116	0.58
20_E	23_K	1.107	0.57
110_I	120_V	1.106	0.57
166_V	170_G	1.106	0.57
62_V	67_Q	1.094	0.57
8_C	15_Y	1.086	0.56
57_I	89_I	1.081	0.56
22_V	39_F	1.069	0.55
14_S	172_L	1.065	0.55
67_Q	70_D	1.062	0.54
58_L	82_V	1.049	0.53
56_V	119_V	1.042	0.53
173_Y	176_R	1.032	0.52
56_V	93_G	1.028	0.52
62_V	71_L	1.027	0.52
89_I	106_L	1.018	0.51
27_I	134_L	1.017	0.51
54_R	142_N	0.999	0.50
164_I	174_G	0.999	0.50
127_V	134_L	0.996	0.50
43_D	46_R	0.981	0.49
117_R	146_V	0.975	0.48
28_K	43_D	0.975	0.48
75_V	107_L	0.957	0.47
138_C	169_S	0.956	0.47
81_Q	146_V	0.949	0.46
8_C	18_L	0.941	0.46
26_Y	34_S	0.932	0.45
131_F	134_L	0.920	0.44
13_I	20_E	0.919	0.44
159_E	170_G	0.917	0.44
91_D	121_T	0.909	0.43
95_K	103_F	0.904	0.43
129_F	160_D	0.892	0.42
51_I	147_V	0.891	0.42
170_G	177_S	0.887	0.42
3_R	33_Q	0.878	0.41
171_K	175_M	0.877	0.41
158_V	161_L	0.863	0.40
110_I	143_C	0.863	0.40
161_L	165_L	0.858	0.40
27_I	43_D	0.855	0.40
11_L	18_L	0.852	0.39
22_V	31_I	0.851	0.39
82_V	85_Y	0.843	0.39
18_L	22_V	0.842	0.39
68_K	179_E	0.833	0.38
98_M	127_V	0.830	0.38
67_Q	73_N	0.829	0.38
4_P	15_Y	0.829	0.38
19_R	39_F	0.822	0.37
42_E	73_N	0.818	0.37
8_C	171_K	0.818	0.37
4_P	29_P	0.813	0.37
5_K	34_S	0.811	0.37
2_L	11_L	0.806	0.36
4_P	7_A	0.806	0.36
19_R	22_V	0.803	0.36
39_F	43_D	0.801	0.36
77_Y	149_N	0.797	0.36
64_S	73_N	0.795	0.36
44_V	118_V	0.789	0.35
155_E	158_V	0.786	0.35
73_N	162_V	0.786	0.35
7_A	18_L	0.783	0.35
134_L	138_C	0.780	0.35
157_L	166_V	0.780	0.35
97_N	141_H	0.770	0.34
103_F	127_V	0.755	0.33
72_V	75_V	0.754	0.33
156_E	168_L	0.754	0.33
33_Q	113_N	0.751	0.33
120_V	145_I	0.751	0.33
64_S	67_Q	0.744	0.32
63_S	67_Q	0.737	0.32
111_L	137_V	0.732	0.32
93_G	163_S	0.731	0.31
19_R	31_I	0.731	0.31
118_V	145_I	0.728	0.31
54_R	143_C	0.723	0.31
166_V	178_H	0.721	0.31
84_E	119_V	0.716	0.31
30_V	38_R	0.716	0.31
81_Q	149_N	0.715	0.30
57_I	106_L	0.712	0.30
78_L	143_C	0.712	0.30
97_N	128_R	0.709	0.30
121_T	166_V	0.709	0.30
6_E	146_V	0.701	0.30
85_Y	148_L	0.699	0.30
79_E	88_V	0.698	0.29
118_V	171_K	0.697	0.29
70_D	121_T	0.690	0.29
97_N	106_L	0.687	0.29
7_A	38_R	0.684	0.29
108_R	140_A	0.681	0.28
148_L	154_E	0.681	0.28
87_L	100_R	0.681	0.28
153_K	175_M	0.680	0.28
120_V	147_V	0.679	0.28
147_V	165_L	0.675	0.28
120_V	143_C	0.673	0.28
130_G	169_S	0.667	0.28
99_K	171_K	0.665	0.28
19_R	36_K	0.664	0.27
100_R	127_V	0.663	0.27
48_M	51_I	0.653	0.27
134_L	157_L	0.647	0.27
14_S	22_V	0.645	0.26
26_Y	165_L	0.645	0.26
56_V	116_S	0.640	0.26
120_V	160_D	0.637	0.26
107_L	146_V	0.635	0.26
101_K	154_E	0.631	0.26
103_F	167_S	0.631	0.26
6_E	157_L	0.630	0.26
160_D	174_G	0.628	0.25
139_K	171_K	0.626	0.25
8_C	103_F	0.622	0.25
31_I	36_K	0.621	0.25
92_I	145_I	0.620	0.25
161_L	164_I	0.618	0.25
77_Y	163_S	0.617	0.25
96_L	133_I	0.617	0.25
6_E	43_D	0.617	0.25
119_V	181_E	0.617	0.25
85_Y	96_L	0.616	0.25
63_S	128_R	0.614	0.25
30_V	43_D	0.614	0.25
66_T	122_Y	0.610	0.25
9_Q	68_K	0.609	0.24
19_R	37_W	0.609	0.24
86_D	148_L	0.606	0.24
92_I	100_R	0.605	0.24
64_S	120_V	0.605	0.24
3_R	36_K	0.602	0.24
107_L	118_V	0.601	0.24
20_E	29_P	0.600	0.24
57_I	150_Q	0.600	0.24
129_F	174_G	0.600	0.24
54_R	112_N	0.600	0.24
130_G	146_V	0.597	0.24
46_R	122_Y	0.597	0.24
118_V	126_L	0.595	0.24
119_V	136_E	0.594	0.24
126_L	133_I	0.592	0.24
22_V	32_L	0.589	0.23
160_D	164_I	0.588	0.23
55_K	116_S	0.587	0.23
4_P	8_C	0.585	0.23
81_Q	85_Y	0.585	0.23
103_F	116_S	0.579	0.23
85_Y	121_T	0.579	0.23
91_D	145_I	0.575	0.23
57_I	115_V	0.575	0.23
178_H	182_K	0.573	0.23
161_L	174_G	0.573	0.23
36_K	39_F	0.570	0.22
23_K	31_I	0.569	0.22
129_F	164_I	0.568	0.22
45_E	48_M	0.568	0.22
66_T	121_T	0.565	0.22
115_V	143_C	0.565	0.22
104_L	134_L	0.564	0.22
6_E	57_I	0.564	0.22
13_I	16_A	0.562	0.22
175_M	182_K	0.561	0.22
123_S	165_L	0.560	0.22
76_K	80_E	0.557	0.22
16_A	20_E	0.555	0.22
2_L	22_V	0.555	0.22
69_D	164_I	0.552	0.22
2_L	13_I	0.552	0.22
15_Y	37_W	0.552	0.22
77_Y	91_D	0.552	0.22
23_K	101_K	0.551	0.22
65_N	113_N	0.549	0.21
110_I	147_V	0.549	0.21
181_E	184_K	0.548	0.21
119_V	134_L	0.548	0.21
177_S	180_Y	0.547	0.21
39_F	44_V	0.546	0.21
12_G	129_F	0.540	0.21
157_L	160_D	0.539	0.21
70_D	132_E	0.538	0.21
165_L	169_S	0.536	0.21
34_S	113_N	0.531	0.21
81_Q	148_L	0.529	0.20
5_K	25_G	0.520	0.20
98_M	118_V	0.519	0.20
145_I	148_L	0.515	0.20
55_K	91_D	0.515	0.20
137_V	140_A	0.513	0.20
98_M	103_F	0.512	0.20
41_E	49_G	0.510	0.20
73_N	76_K	0.505	0.19
31_I	40_R	0.503	0.19
142_N	161_L	0.503	0.19
91_D	98_M	0.500	0.19

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3ilxA	2	0.7077	100	0.655	Contact Map
3lhkA	2	0.7231	99.9	0.663	Contact Map
3g13A	2	0.7179	99.8	0.74	Contact Map
3bvpA	4	0.6154	99.8	0.748	Contact Map
4bqqA	2	0.7026	99.8	0.748	Contact Map
2mhcA	1	0.5538	99.8	0.75	Contact Map
3guvA	2	0.6769	99.8	0.752	Contact Map
2gm5A	4	0.6051	99.8	0.759	Contact Map
3pkzA	4	0.5744	99.7	0.765	Contact Map
1gdtA	2	0.6923	99.7	0.766	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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