Q97YW4 - SULSO - GREMLIN Contacts

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Q97YW4 - Transposase ISC1316

UniProt:	Q97YW4	Sequences: MPTLGFRFRAYTDEQTLRALKAQLKLTCEIYNTLRWADIYFYQRDGKGLTQTELRQLALDLRKQDDEYKQLYSQVVQQVADRYSEAKKRFFEGLARFPKEKKPHKYYSLVYTQSGWKILHVREIRKGKKNKKKLITLKLSNLGTFKVIVHRDFPLDKVKRVVVKLTRSERIYITFVVDHEFPKLPNTGKVVAIDVGVEKLLITSDGEYFPNLRPYEKALWKVKHIHRELSRKKFLSNNWFKAKVKLARAYEHLKNLRTDLYMKLGKWFAEHYDVVVMEGIHAKQLVGKSLRSLRRRLSDVGFGELRGVLKYQLEKYGKKLILVNPAYTSKTCARCGYVKNDLSLSDRVFVCPNCGWIADRDYNASLNILRGSGSERPLVWSSALYQYSGKVGL	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	393 (351)
Sequences:	4613 (2940)
Seq/√Len:	156.9

ECOLI - INSQ
HALSA - Q9HSW9 Q9HHJ9 Q9HM87 Q9HHG8 Q9HSW7 Q9HSD4 Q9HSZ6 Q9HHU6 Q9HSY4 Q9HHX8
SULSO - Q97YW4 Q97XQ8 Q97Y44 Q97V25 Q97XP1 Q97V47 Q97YI2 Q97XA4 Q97V53 Q97Y48 Q97YI5 Q97YU1 Q97XB6 Q97U68 Q97Z61 Q97U51 Q97XQ0 Q97WY6 Q97UQ8 Q97Y67 Q97YW9 Q7LXD9 Q97X39 Q97WZ3 Q7LXH2 Q7LXH6 Q97YF7 Q97U09 Q7LX59 Q7LX75 Y2105 Q97YK6 Q7LXH9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
259_D	263_K	5.468	1.00
281_H	284_Q	4.323	1.00
333_A	363_N	4.245	1.00
313_L	320_L	3.646	1.00
276_V	372_S	3.074	1.00
200_L	305_L	2.997	1.00
277_M	309_L	2.943	1.00
306_R	322_L	2.879	1.00
266_K	270_E	2.758	1.00
304_E	308_V	2.692	1.00
343_S	346_D	2.648	1.00
161_V	173_I	2.536	1.00
286_V	298_S	2.461	1.00
327_Y	330_K	2.375	1.00
219_L	295_R	2.362	1.00
304_E	307_G	2.340	1.00
331_T	338_V	2.340	1.00
310_K	322_L	2.318	1.00
305_L	309_L	2.261	1.00
323_V	368_I	2.257	1.00
269_A	313_L	2.246	1.00
371_G	374_S	2.211	1.00
275_V	313_L	2.188	1.00
330_K	342_L	2.139	1.00
350_V	357_I	2.138	1.00
13_D	16_T	2.088	1.00
328_T	368_I	2.079	1.00
225_I	242_A	2.074	1.00
159_K	178_D	2.073	1.00
277_M	320_L	2.062	1.00
262_M	311_Y	2.051	1.00
262_M	308_V	2.050	1.00
332_C	335_C	2.028	1.00
335_C	354_C	2.015	1.00
291_R	294_R	2.008	1.00
258_T	304_E	1.988	1.00
218_A	256_L	1.986	1.00
347_R	360_R	1.943	1.00
200_L	260_L	1.932	1.00
39_I	97_F	1.924	1.00
265_G	308_V	1.913	1.00
51_Q	55_R	1.898	1.00
262_M	265_G	1.851	1.00
335_C	351_C	1.843	1.00
242_A	246_L	1.839	1.00
21_K	165_L	1.838	1.00
34_L	80_A	1.834	1.00
351_C	354_C	1.833	1.00
262_M	312_Q	1.826	1.00
261_Y	308_V	1.804	1.00
22_A	70_Q	1.782	1.00
332_C	354_C	1.772	1.00
273_D	318_K	1.772	1.00
310_K	314_E	1.760	1.00
36_W	40_Y	1.754	1.00
334_R	356_W	1.732	1.00
349_F	358_A	1.729	1.00
21_K	171_I	1.728	1.00
237_N	240_F	1.720	1.00
35_R	83_Y	1.711	1.00
52_T	55_R	1.704	1.00
332_C	351_C	1.693	1.00
9_R	170_R	1.654	1.00
82_R	110_V	1.644	1.00
20_L	171_I	1.615	1.00
137_L	173_I	1.597	1.00
193_I	201_L	1.597	1.00
277_M	322_L	1.595	1.00
261_Y	304_E	1.592	1.00
29_E	67_E	1.578	1.00
240_F	243_K	1.567	1.00
232_K	242_A	1.551	1.00
51_Q	81_D	1.548	1.00
342_L	346_D	1.525	1.00
89_R	95_A	1.524	1.00
229_L	243_K	1.521	1.00
74_Q	112_T	1.511	1.00
339_K	342_L	1.504	1.00
265_G	311_Y	1.503	1.00
277_M	306_R	1.498	1.00
228_E	232_K	1.485	1.00
348_V	357_I	1.475	1.00
279_G	322_L	1.475	1.00
74_Q	113_Q	1.473	1.00
18_R	22_A	1.472	1.00
190_V	369_L	1.465	1.00
349_F	356_W	1.458	1.00
339_K	349_F	1.433	1.00
17_L	21_K	1.420	1.00
202_I	362_Y	1.406	1.00
235_L	239_W	1.406	1.00
84_S	88_K	1.392	1.00
344_L	347_R	1.368	1.00
278_E	325_P	1.365	1.00
203_T	207_E	1.346	1.00
191_V	203_T	1.325	1.00
251_E	255_N	1.320	1.00
170_R	251_E	1.314	1.00
199_K	202_I	1.310	1.00
366_L	370_R	1.309	1.00
265_G	312_Q	1.299	1.00
339_K	352_P	1.297	1.00
5_G	174_T	1.283	1.00
337_Y	352_P	1.279	1.00
265_G	305_L	1.274	1.00
225_I	228_E	1.273	1.00
310_K	320_L	1.271	1.00
24_L	171_I	1.269	1.00
55_R	81_D	1.262	1.00
222_V	250_Y	1.260	1.00
330_K	341_D	1.259	1.00
168_S	251_E	1.255	1.00
297_L	302_F	1.253	1.00
218_A	253_L	1.252	1.00
39_I	43_Q	1.238	1.00
34_L	83_Y	1.230	1.00
87_K	97_F	1.228	1.00
221_K	225_I	1.209	1.00
52_T	56_Q	1.204	1.00
269_A	316_Y	1.198	1.00
333_A	366_L	1.196	1.00
222_V	246_L	1.178	1.00
51_Q	80_A	1.176	1.00
225_I	249_A	1.171	1.00
191_V	272_Y	1.164	1.00
38_D	54_L	1.152	1.00
14_E	17_L	1.139	1.00
27_T	79_V	1.139	1.00
190_V	274_V	1.138	1.00
12_T	16_T	1.136	1.00
343_S	347_R	1.133	1.00
195_V	302_F	1.128	1.00
261_Y	265_G	1.127	1.00
164_K	174_T	1.126	1.00
358_A	366_L	1.123	1.00
88_K	92_E	1.122	1.00
281_H	325_P	1.118	1.00
267_W	271_H	1.114	1.00
78_Q	81_D	1.112	1.00
343_S	360_R	1.111	1.00
362_Y	366_L	1.109	1.00
330_K	338_V	1.108	1.00
78_Q	112_T	1.108	1.00
85_E	89_R	1.107	1.00
266_K	316_Y	1.106	1.00
193_I	203_T	1.099	0.99
344_L	360_R	1.099	0.99
50_T	53_E	1.099	0.99
333_A	358_A	1.098	0.99
308_V	311_Y	1.090	0.99
229_L	246_L	1.089	0.99
192_A	369_L	1.089	0.99
261_Y	305_L	1.079	0.99
229_L	242_A	1.078	0.99
323_V	371_G	1.074	0.99
197_V	300_V	1.072	0.99
55_R	80_A	1.067	0.99
312_Q	315_K	1.061	0.99
37_A	57_L	1.061	0.99
56_Q	59_L	1.061	0.99
40_Y	44_R	1.059	0.99
269_A	318_K	1.059	0.99
58_A	77_Q	1.053	0.99
265_G	309_L	1.038	0.99
258_T	308_V	1.035	0.99
187_T	273_D	1.034	0.99
226_H	230_S	1.031	0.99
72_Y	75_V	1.024	0.99
6_F	177_V	1.021	0.99
286_V	294_R	1.011	0.99
270_E	316_Y	1.007	0.99
269_A	275_V	0.994	0.99
342_L	349_F	0.992	0.99
117_K	120_H	0.983	0.99
331_T	371_G	0.981	0.99
333_A	364_A	0.980	0.99
339_K	343_S	0.978	0.99
78_Q	110_V	0.978	0.99
58_A	80_A	0.977	0.99
239_W	243_K	0.975	0.99
313_L	318_K	0.971	0.99
115_G	140_S	0.971	0.99
35_R	87_K	0.968	0.99
31_Y	79_V	0.967	0.99
74_Q	114_S	0.966	0.99
139_L	163_V	0.964	0.99
110_V	160_R	0.961	0.99
204_S	208_Y	0.958	0.99
358_A	362_Y	0.957	0.99
346_D	360_R	0.950	0.98
328_T	371_G	0.947	0.98
195_V	306_R	0.946	0.98
208_Y	359_D	0.943	0.98
8_F	173_I	0.939	0.98
29_E	33_T	0.938	0.98
225_I	246_L	0.934	0.98
62_R	76_V	0.933	0.98
199_K	210_P	0.932	0.98
41_F	45_D	0.929	0.98
323_V	372_S	0.928	0.98
90_F	98_P	0.925	0.98
111_Y	114_S	0.920	0.98
26_L	67_E	0.919	0.98
282_A	303_G	0.918	0.98
254_K	258_T	0.916	0.98
51_Q	54_L	0.914	0.98
222_V	253_L	0.914	0.98
110_V	162_V	0.912	0.98
333_A	356_W	0.909	0.98
364_A	367_N	0.907	0.98
138_K	144_T	0.904	0.98
74_Q	78_Q	0.903	0.98
347_R	359_D	0.901	0.98
116_W	120_H	0.900	0.98
190_V	373_G	0.898	0.98
149_V	152_D	0.896	0.98
346_D	349_F	0.895	0.98
89_R	94_L	0.894	0.98
90_F	97_F	0.893	0.98
337_Y	353_N	0.891	0.98
279_G	325_P	0.890	0.98
77_Q	81_D	0.888	0.98
104_H	107_Y	0.888	0.98
199_K	208_Y	0.884	0.98
12_T	20_L	0.882	0.98
362_Y	365_S	0.882	0.98
232_K	238_N	0.881	0.98
325_P	328_T	0.880	0.98
348_V	358_A	0.877	0.97
261_Y	301_G	0.875	0.97
245_K	248_R	0.874	0.97
82_R	162_V	0.873	0.97
307_G	310_K	0.872	0.97
31_Y	86_A	0.871	0.97
223_K	226_H	0.869	0.97
39_I	87_K	0.865	0.97
136_T	144_T	0.864	0.97
89_R	92_E	0.863	0.97
276_V	368_I	0.859	0.97
252_H	256_L	0.858	0.97
25_K	28_C	0.858	0.97
349_F	360_R	0.855	0.97
134_L	139_L	0.848	0.97
222_V	230_S	0.847	0.97
31_Y	83_Y	0.847	0.97
53_E	56_Q	0.846	0.97
249_A	253_L	0.845	0.97
303_G	307_G	0.842	0.97
197_V	286_V	0.838	0.97
68_Y	76_V	0.836	0.97
363_N	367_N	0.835	0.97
207_E	267_W	0.833	0.97
33_T	37_A	0.829	0.96
230_S	246_L	0.827	0.96
34_L	54_L	0.827	0.96
342_L	360_R	0.826	0.96
264_L	267_W	0.826	0.96
195_V	201_L	0.824	0.96
17_L	171_I	0.821	0.96
229_L	239_W	0.821	0.96
202_I	208_Y	0.820	0.96
226_H	246_L	0.819	0.96
293_L	297_L	0.819	0.96
162_V	174_T	0.816	0.96
41_F	49_L	0.814	0.96
260_L	264_L	0.814	0.96
214_P	256_L	0.813	0.96
190_V	276_V	0.813	0.96
366_L	369_L	0.807	0.96
333_A	370_R	0.804	0.96
266_K	312_Q	0.804	0.96
36_W	100_E	0.802	0.96
163_V	173_I	0.802	0.96
337_Y	349_F	0.801	0.96
42_Y	87_K	0.796	0.96
222_V	226_H	0.793	0.95
25_K	29_E	0.791	0.95
71_L	74_Q	0.791	0.95
268_F	275_V	0.788	0.95
218_A	222_V	0.788	0.95
189_K	273_D	0.787	0.95
59_L	62_R	0.783	0.95
226_H	250_Y	0.782	0.95
229_L	232_K	0.782	0.95
188_G	273_D	0.780	0.95
160_R	176_V	0.780	0.95
38_D	84_S	0.779	0.95
23_Q	27_T	0.779	0.95
34_L	58_A	0.775	0.95
93_G	96_R	0.772	0.95
74_Q	77_Q	0.772	0.95
330_K	339_K	0.771	0.95
225_I	245_K	0.770	0.95
274_V	321_I	0.765	0.94
274_V	319_K	0.765	0.94
26_L	68_Y	0.764	0.94
291_R	298_S	0.764	0.94
37_A	54_L	0.763	0.94
191_V	204_S	0.758	0.94
78_Q	82_R	0.757	0.94
243_K	247_A	0.757	0.94
214_P	218_A	0.756	0.94
137_L	161_V	0.751	0.94
228_E	231_R	0.750	0.94
165_L	171_I	0.748	0.94
191_V	275_V	0.747	0.94
197_V	296_R	0.747	0.94
59_L	63_K	0.746	0.94
279_G	306_R	0.746	0.94
224_H	228_E	0.745	0.94
327_Y	371_G	0.744	0.94
211_N	214_P	0.740	0.93
334_R	366_L	0.736	0.93
223_K	227_R	0.736	0.93
10_A	173_I	0.735	0.93
30_I	79_V	0.735	0.93
198_E	202_I	0.734	0.93
7_R	164_K	0.731	0.93
276_V	321_I	0.727	0.93
11_Y	148_I	0.723	0.93
8_F	151_R	0.722	0.93
209_F	260_L	0.720	0.92
117_K	138_K	0.719	0.92
280_I	325_P	0.716	0.92
12_T	145_F	0.716	0.92
139_L	145_F	0.715	0.92
218_A	249_A	0.714	0.92
215_Y	218_A	0.710	0.92
331_T	367_N	0.708	0.92
201_L	264_L	0.702	0.92
284_Q	325_P	0.702	0.92
119_L	138_K	0.702	0.92
136_T	139_L	0.701	0.91
186_N	189_K	0.701	0.91
139_L	142_L	0.700	0.91
196_G	202_I	0.699	0.91
211_N	300_V	0.698	0.91
42_Y	46_G	0.697	0.91
157_K	178_D	0.697	0.91
327_Y	331_T	0.696	0.91
268_F	272_Y	0.692	0.91
26_L	71_L	0.691	0.91
194_D	361_D	0.690	0.91
223_K	230_S	0.688	0.91
342_L	347_R	0.687	0.91
74_Q	115_G	0.685	0.91
19_A	23_Q	0.685	0.91
218_A	252_H	0.684	0.90
87_K	91_F	0.684	0.90
14_E	18_R	0.682	0.90
238_N	241_K	0.680	0.90
282_A	302_F	0.680	0.90
235_L	240_F	0.678	0.90
181_F	314_E	0.675	0.90
275_V	309_L	0.668	0.89
80_A	84_S	0.665	0.89
204_S	362_Y	0.664	0.89
253_L	299_D	0.664	0.89
79_V	83_Y	0.663	0.89
184_L	187_T	0.662	0.89
339_K	346_D	0.662	0.89
201_L	209_F	0.661	0.89
38_D	87_K	0.660	0.89
147_V	150_H	0.660	0.89
22_A	26_L	0.659	0.89
138_K	142_L	0.658	0.89
321_I	372_S	0.655	0.88
83_Y	87_K	0.651	0.88
201_L	305_L	0.650	0.88
341_D	346_D	0.649	0.88
40_Y	43_Q	0.647	0.88
87_K	90_F	0.646	0.88
189_K	205_D	0.644	0.88
327_Y	338_V	0.644	0.88
335_C	339_K	0.640	0.87
358_A	363_N	0.639	0.87
20_L	24_L	0.635	0.87
208_Y	362_Y	0.634	0.87
26_L	29_E	0.634	0.87
233_K	236_S	0.634	0.87
313_L	316_Y	0.633	0.87
5_G	164_K	0.632	0.87
344_L	359_D	0.632	0.87
72_Y	111_Y	0.631	0.87
38_D	48_G	0.626	0.86
311_Y	315_K	0.626	0.86
71_L	76_V	0.625	0.86
200_L	300_V	0.623	0.86
368_I	371_G	0.623	0.86
107_Y	165_L	0.623	0.86
134_L	138_K	0.622	0.86
204_S	365_S	0.619	0.86
216_E	219_L	0.618	0.85
216_E	295_R	0.617	0.85
81_D	112_T	0.617	0.85
48_G	54_L	0.617	0.85
308_V	312_Q	0.617	0.85
45_D	48_G	0.615	0.85
19_A	22_A	0.615	0.85
37_A	41_F	0.613	0.85
15_Q	18_R	0.611	0.85
284_Q	297_L	0.610	0.85
170_R	255_N	0.610	0.85
337_Y	341_D	0.610	0.85
285_L	297_L	0.608	0.85
276_V	369_L	0.607	0.84
255_N	258_T	0.604	0.84
111_Y	115_G	0.604	0.84
184_L	318_K	0.603	0.84
250_Y	257_R	0.603	0.84
228_E	242_A	0.602	0.84
60_D	64_Q	0.601	0.84
5_G	176_V	0.599	0.84
270_E	274_V	0.598	0.84
233_K	239_W	0.598	0.84
257_R	299_D	0.597	0.83
6_F	151_R	0.596	0.83
284_Q	293_L	0.595	0.83
200_L	265_G	0.595	0.83
216_E	220_W	0.595	0.83
215_Y	219_L	0.594	0.83
309_L	320_L	0.594	0.83
192_A	276_V	0.593	0.83
86_A	98_P	0.593	0.83
109_L	113_Q	0.592	0.83
23_Q	109_L	0.589	0.83
272_Y	275_V	0.589	0.83
339_K	360_R	0.588	0.83
236_S	239_W	0.587	0.82
9_R	172_Y	0.585	0.82
200_L	261_Y	0.583	0.82
37_A	40_Y	0.582	0.82
23_Q	111_Y	0.581	0.82
111_Y	139_L	0.579	0.82
36_W	39_I	0.578	0.81
350_V	356_W	0.577	0.81
194_D	329_S	0.577	0.81
116_W	161_V	0.576	0.81
334_R	358_A	0.576	0.81
209_F	267_W	0.574	0.81
55_R	59_L	0.574	0.81
42_Y	90_F	0.572	0.81
306_R	310_K	0.571	0.81
223_K	291_R	0.570	0.81
198_E	347_R	0.570	0.81
108_S	162_V	0.568	0.80
189_K	272_Y	0.566	0.80
73_S	112_T	0.561	0.79
116_W	140_S	0.560	0.79
227_R	230_S	0.559	0.79
331_T	336_G	0.559	0.79
29_E	65_D	0.558	0.79
331_T	337_Y	0.556	0.79
209_F	264_L	0.556	0.79
54_L	58_A	0.556	0.79
216_E	296_R	0.556	0.79
326_A	330_K	0.555	0.79
15_Q	19_A	0.555	0.79
342_L	345_S	0.553	0.79
370_R	373_G	0.553	0.79
205_D	272_Y	0.552	0.78
31_Y	35_R	0.552	0.78
75_V	109_L	0.552	0.78
50_T	55_R	0.551	0.78
12_T	17_L	0.550	0.78
54_L	57_L	0.550	0.78
22_A	25_K	0.547	0.78
294_R	298_S	0.547	0.78
301_G	304_E	0.547	0.78
139_L	161_V	0.545	0.78
261_Y	264_L	0.545	0.78
75_V	111_Y	0.543	0.77
293_L	296_R	0.543	0.77
276_V	371_G	0.541	0.77
49_L	54_L	0.541	0.77
329_S	343_S	0.540	0.77
57_L	61_L	0.539	0.77
164_K	167_R	0.537	0.77
59_L	80_A	0.537	0.77
23_Q	72_Y	0.535	0.76
142_L	145_F	0.535	0.76
258_T	262_M	0.534	0.76
73_S	115_G	0.532	0.76
195_V	282_A	0.531	0.76
262_M	266_K	0.531	0.76
352_P	360_R	0.531	0.76
80_A	83_Y	0.530	0.76
203_T	268_F	0.530	0.76
33_T	67_E	0.529	0.75
224_H	227_R	0.527	0.75
192_A	365_S	0.526	0.75
72_Y	113_Q	0.526	0.75
286_V	297_L	0.525	0.75
72_Y	109_L	0.525	0.75
276_V	373_G	0.523	0.75
8_F	149_V	0.523	0.75
228_E	245_K	0.522	0.74
219_L	299_D	0.521	0.74
23_Q	71_L	0.521	0.74
359_D	364_A	0.520	0.74
253_L	257_R	0.519	0.74
59_L	73_S	0.519	0.74
200_L	309_L	0.519	0.74
41_F	47_K	0.518	0.74
192_A	204_S	0.517	0.74
53_E	57_L	0.516	0.74
111_Y	118_I	0.516	0.74
76_V	79_V	0.515	0.74
324_N	327_Y	0.514	0.73
20_L	109_L	0.514	0.73
48_G	84_S	0.513	0.73
250_Y	254_K	0.513	0.73
264_L	305_L	0.511	0.73
151_R	154_P	0.510	0.73
214_P	299_D	0.509	0.73
183_K	314_E	0.508	0.73
330_K	343_S	0.507	0.72
269_A	309_L	0.507	0.72
196_G	199_K	0.506	0.72
331_T	349_F	0.505	0.72
217_K	220_W	0.505	0.72
38_D	45_D	0.505	0.72
266_K	308_V	0.502	0.72
116_W	158_V	0.502	0.72
231_R	242_A	0.502	0.72
38_D	41_F	0.500	0.71

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1vhxA	2	0.3486	93.5	0.932	Contact Map
1nu0A	1	0.3308	92.8	0.934	Contact Map
1iv0A	1	0.2468	88.8	0.94	Contact Map
4aybP	1	0.1094	87.8	0.941	Contact Map
1ffkW	1	0.1705	84.9	0.943	Contact Map
1vq8Z	1	0.1756	84.1	0.944	Contact Map
1qypA	1	0.117	83.5	0.944	Contact Map
2k4xA	1	0.1399	82.8	0.945	Contact Map
3j20Y	1	0.1272	82.6	0.945	Contact Map
1vd4A	1	0.1425	81.7	0.945	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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