Q97YI5 - SULSO - GREMLIN Contacts

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Q97YI5 - Second ORF in transposon ISC1904

UniProt:	Q97YI5	Sequences: MLKNLRLKSFQPEEEYVHFTYSIKNDKREESKELIKEYTTLLQKAIDYLWSLTKIQVRKNGNYKITLPKKKEVYKPLRDELEKINHLASHYVDKAINDAFSILKSWRKRAIKGRASIEKPRVKKAYVRIKTTLRKVVGESVRITVRPYEYITFSWSKSWFSRRVRELELGEPIIKEEKVYLPFRYKLPWATPLNFLAIDSNLYTLDAYDGEKFVTISLKQLYSLKYSMEVKRAKVQSFASKHTKRGRELLRKYSHRERNRVLDFVHKFVNALLDLYPMTFFAVEKLNKESMFVDANDSLSRKISRTVWRSIHRVLKYKAPLYGSFVKEVNPRFTSRSCPRCGFVSRKVGKTFECERCGFKLDRQLNASLNIYLKMCGFPHIRDIARVWVGVIPLMGRRGMNGFPRDFGEAQGLRIDIKYHEIP	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	423 (346)
Sequences:	4384 (2779)
Seq/√Len:	149.4

ECOLI - INSQ
HALSA - Q9HSW9 Q9HHJ9 Q9HM87 Q9HSW7 Q9HHG8 Q9HSZ6 Q9HSD4 Q9HHU6 Q9HSY4 Q9HHX8
SULSO - Q97YI5 Q97U68 Q97UQ8 Q97YU1 Q97XQ0 Q97U51 Q97XB6 Q97Y48 Q97Z61 Q97YW9 Q97XQ8 Q97YI2 Q97V25 Q97V47 Q97XP1 Q97Y44 Q97WZ3 Q97YW4 Q97X39 Q97Y67 Q7LXH2 Q7LXD9 Q97V53 Q97XA4 Q97WY6 Q7LXH6 Q97YF7 Y2105 Q97U09 Q7LX59

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
263_D	267_K	5.398	1.00
287_N	290_S	4.400	1.00
339_P	366_N	4.147	1.00
319_A	326_V	3.670	1.00
282_A	375_M	3.084	1.00
205_L	311_I	3.076	1.00
312_H	328_E	3.005	1.00
283_V	315_L	2.896	1.00
346_R	349_G	2.889	1.00
310_S	314_V	2.805	1.00
19_F	321_L	2.769	1.00
270_N	274_D	2.729	1.00
310_S	313_R	2.430	1.00
337_S	344_V	2.425	1.00
172_P	181_L	2.418	1.00
336_R	345_S	2.408	1.00
316_K	328_E	2.394	1.00
311_I	315_L	2.373	1.00
333_F	336_R	2.340	1.00
329_V	371_I	2.268	1.00
273_L	319_A	2.265	1.00
225_K	301_R	2.250	1.00
292_F	304_S	2.207	1.00
374_K	377_G	2.134	1.00
281_F	319_A	2.120	1.00
170_G	186_K	2.082	1.00
266_H	314_V	2.082	1.00
231_K	246_G	2.075	1.00
283_V	326_V	2.066	1.00
28_R	31_S	2.057	1.00
266_H	317_Y	2.056	1.00
334_T	371_I	2.042	1.00
224_L	260_R	2.018	1.00
296_N	300_S	1.996	1.00
353_E	360_K	1.982	1.00
54_K	119_K	1.963	1.00
341_C	357_C	1.960	1.00
246_G	250_L	1.907	1.00
297_D	300_S	1.902	1.00
205_L	264_F	1.885	1.00
262_L	310_S	1.883	1.00
36_K	179_V	1.882	1.00
49_L	96_I	1.868	1.00
316_K	320_P	1.864	1.00
37_E	86_H	1.853	1.00
269_V	314_V	1.821	1.00
350_K	363_R	1.807	1.00
345_S	349_G	1.780	1.00
338_C	341_C	1.770	1.00
354_C	357_C	1.750	1.00
265_V	314_V	1.749	1.00
266_H	318_K	1.745	1.00
352_F	361_L	1.745	1.00
51_S	55_I	1.744	1.00
341_C	354_C	1.730	1.00
50_W	99_A	1.730	1.00
338_C	357_C	1.726	1.00
340_R	359_F	1.720	1.00
277_P	324_S	1.717	1.00
241_K	244_K	1.706	1.00
266_H	269_V	1.703	1.00
244_K	247_R	1.654	1.00
20_T	182_P	1.624	1.00
283_V	328_E	1.622	1.00
231_K	234_K	1.609	1.00
265_V	310_S	1.607	1.00
338_C	354_C	1.560	1.00
35_I	179_V	1.549	1.00
198_I	206_D	1.546	1.00
44_K	83_K	1.537	1.00
285_K	328_E	1.512	1.00
67_L	71_K	1.499	1.00
207_A	365_L	1.498	1.00
33_E	37_E	1.490	1.00
195_F	372_Y	1.483	1.00
100_F	104_K	1.477	1.00
143_I	181_L	1.472	1.00
283_V	312_H	1.457	1.00
269_V	317_Y	1.440	1.00
343_F	355_E	1.439	1.00
39_T	179_V	1.438	1.00
68_P	71_K	1.438	1.00
351_T	360_K	1.437	1.00
352_F	359_F	1.437	1.00
49_L	99_A	1.430	1.00
235_V	247_R	1.392	1.00
105_S	117_I	1.391	1.00
144_T	150_Y	1.382	1.00
316_K	326_V	1.372	1.00
204_T	207_A	1.370	1.00
32_K	36_K	1.370	1.00
284_E	331_P	1.353	1.00
269_V	318_K	1.314	1.00
103_L	119_K	1.311	1.00
255_H	259_N	1.308	1.00
196_L	208_Y	1.307	1.00
369_L	373_L	1.301	1.00
208_Y	212_K	1.294	1.00
347_K	350_K	1.277	1.00
231_K	253_Y	1.260	1.00
195_F	280_F	1.252	1.00
110_A	117_I	1.251	1.00
109_R	117_I	1.245	1.00
90_H	130_K	1.244	1.00
303_I	308_W	1.240	1.00
104_K	108_K	1.225	1.00
346_R	350_K	1.219	1.00
216_I	219_K	1.214	1.00
69_K	72_E	1.202	1.00
42_L	95_A	1.197	1.00
29_E	32_K	1.195	1.00
228_M	250_L	1.193	1.00
365_L	369_L	1.189	1.00
54_K	58_R	1.189	1.00
239_A	243_T	1.186	1.00
224_L	257_E	1.182	1.00
265_V	311_I	1.177	1.00
273_L	322_Y	1.172	1.00
90_H	132_T	1.170	1.00
196_L	276_Y	1.168	1.00
271_A	275_L	1.166	1.00
94_K	97_N	1.158	1.00
176_E	255_H	1.155	1.00
287_N	331_P	1.153	1.00
178_K	255_H	1.153	1.00
339_P	369_L	1.136	1.00
228_M	254_S	1.134	1.00
339_P	361_L	1.129	0.99
72_E	75_K	1.123	0.99
197_A	372_Y	1.122	0.99
78_R	92_V	1.117	0.99
101_S	105_S	1.116	0.99
200_S	308_W	1.108	0.99
314_V	317_Y	1.107	0.99
213_F	362_D	1.107	0.99
269_V	311_I	1.105	0.99
55_I	59_K	1.101	0.99
98_D	128_R	1.096	0.99
202_L	306_T	1.095	0.99
361_L	369_L	1.090	0.99
262_L	314_V	1.080	0.99
155_W	158_S	1.072	0.99
258_R	262_L	1.068	0.99
270_N	322_Y	1.064	0.99
227_S	231_K	1.063	0.99
46_I	95_A	1.060	0.99
212_K	271_A	1.060	0.99
27_K	35_I	1.057	0.99
137_V	140_S	1.056	0.99
200_S	312_H	1.055	0.99
347_K	363_R	1.053	0.99
198_I	208_Y	1.053	0.99
73_V	77_L	1.048	0.99
65_I	69_K	1.046	0.99
334_T	374_K	1.045	0.99
27_K	31_S	1.044	0.99
269_V	315_L	1.043	0.99
88_A	91_Y	1.038	0.99
105_S	110_A	1.036	0.99
336_R	344_V	1.034	0.99
329_V	374_K	1.025	0.99
265_V	269_V	1.024	0.99
138_G	141_V	1.022	0.99
274_D	322_Y	1.018	0.99
337_S	374_K	1.017	0.99
288_K	309_R	1.014	0.99
273_L	281_F	1.014	0.99
21_Y	185_Y	1.013	0.99
318_K	321_L	1.012	0.99
282_A	371_I	1.010	0.99
195_F	376_C	1.009	0.99
232_R	250_L	1.009	0.99
346_R	363_R	1.003	0.99
127_V	131_T	1.002	0.99
313_R	316_K	1.002	0.99
70_K	73_V	0.998	0.99
345_S	352_F	0.995	0.99
280_F	325_F	0.993	0.99
232_R	236_Q	0.990	0.99
40_T	43_Q	0.988	0.99
231_K	250_L	0.988	0.99
235_V	250_L	0.986	0.99
70_K	74_Y	0.985	0.99
105_S	109_R	0.984	0.99
54_K	103_L	0.979	0.99
207_A	213_F	0.978	0.99
234_K	238_F	0.978	0.99
339_P	359_F	0.977	0.99
285_K	331_P	0.977	0.99
127_V	130_K	0.975	0.99
361_L	365_L	0.972	0.98
339_P	367_A	0.965	0.98
94_K	128_R	0.965	0.98
365_L	368_S	0.951	0.98
50_W	103_L	0.949	0.98
273_L	324_S	0.948	0.98
56_Q	60_N	0.942	0.98
350_K	362_D	0.941	0.98
32_K	179_V	0.940	0.98
329_V	375_M	0.938	0.98
228_M	257_E	0.936	0.98
243_T	247_R	0.933	0.98
74_Y	77_L	0.932	0.98
204_T	215_T	0.923	0.98
44_K	48_Y	0.921	0.98
296_N	299_L	0.919	0.98
196_L	209_D	0.918	0.98
46_I	99_A	0.917	0.98
206_D	268_F	0.911	0.98
192_P	277_P	0.911	0.98
75_K	78_R	0.910	0.98
90_H	94_K	0.906	0.98
272_L	281_F	0.905	0.98
109_R	118_E	0.899	0.97
220_Q	260_R	0.896	0.97
106_W	120_P	0.895	0.97
75_K	79_D	0.895	0.97
46_I	102_I	0.894	0.97
24_K	178_K	0.891	0.97
133_L	146_R	0.890	0.97
71_K	75_K	0.888	0.97
265_V	307_V	0.887	0.97
98_D	173_I	0.886	0.97
343_F	356_R	0.885	0.97
331_P	334_T	0.884	0.97
202_L	302_K	0.883	0.97
106_W	119_K	0.883	0.97
249_L	252_K	0.883	0.97
231_K	249_L	0.877	0.97
340_R	369_L	0.876	0.97
52_L	73_V	0.875	0.97
142_R	150_Y	0.874	0.97
41_L	83_K	0.874	0.97
280_F	327_K	0.873	0.97
240_S	243_T	0.871	0.97
333_F	374_K	0.870	0.97
74_Y	78_R	0.866	0.97
105_S	108_K	0.866	0.97
288_K	308_W	0.859	0.97
58_R	61_G	0.856	0.97
133_L	136_V	0.853	0.97
90_H	131_T	0.849	0.96
351_T	361_L	0.849	0.96
128_R	171_E	0.848	0.96
292_F	296_N	0.847	0.96
200_S	206_D	0.847	0.96
195_F	282_A	0.845	0.96
143_I	172_P	0.843	0.96
76_P	80_E	0.840	0.96
173_I	182_P	0.836	0.96
367_A	370_N	0.831	0.96
53_T	100_F	0.830	0.96
253_Y	257_E	0.829	0.96
93_D	97_N	0.824	0.96
319_A	324_S	0.824	0.96
69_K	74_Y	0.820	0.96
339_P	373_L	0.817	0.96
214_V	264_F	0.817	0.96
270_N	318_K	0.815	0.95
345_S	363_R	0.813	0.95
48_Y	52_L	0.812	0.95
229_E	233_A	0.808	0.95
57_V	61_G	0.808	0.95
343_F	352_F	0.807	0.95
135_K	144_T	0.805	0.95
153_F	156_S	0.799	0.95
66_T	69_K	0.798	0.95
349_G	363_R	0.795	0.95
232_R	254_S	0.794	0.95
369_L	372_Y	0.792	0.95
84_I	92_V	0.790	0.95
292_F	300_S	0.788	0.95
196_L	281_F	0.787	0.95
268_F	271_A	0.787	0.95
145_V	174_I	0.784	0.94
175_K	178_K	0.782	0.94
135_K	138_G	0.782	0.94
290_S	331_P	0.781	0.94
68_P	72_E	0.781	0.94
366_N	370_N	0.779	0.94
333_F	337_S	0.778	0.94
229_E	232_R	0.773	0.94
256_R	260_R	0.772	0.94
94_K	98_D	0.772	0.94
337_S	370_N	0.770	0.94
45_A	95_A	0.767	0.94
194_N	210_G	0.765	0.94
24_K	180_Y	0.765	0.94
69_K	73_V	0.764	0.94
220_Q	224_L	0.761	0.94
69_K	77_L	0.760	0.93
235_V	246_G	0.759	0.93
264_F	268_F	0.757	0.93
247_R	251_R	0.755	0.93
327_K	375_M	0.753	0.93
236_Q	250_L	0.750	0.93
213_F	365_L	0.749	0.93
53_T	103_L	0.749	0.93
374_K	378_F	0.744	0.93
285_K	312_H	0.738	0.92
94_K	130_K	0.738	0.92
352_F	363_R	0.737	0.92
25_N	181_L	0.736	0.92
191_T	194_N	0.735	0.92
103_L	106_W	0.734	0.92
57_V	103_L	0.732	0.92
235_V	243_T	0.728	0.92
23_I	157_K	0.721	0.92
228_M	232_R	0.720	0.92
201_N	207_A	0.717	0.91
282_A	372_Y	0.716	0.91
102_I	120_P	0.716	0.91
272_L	276_Y	0.714	0.91
199_D	364_Q	0.714	0.91
41_L	87_L	0.714	0.91
228_M	236_Q	0.714	0.91
290_S	303_I	0.713	0.91
38_Y	42_L	0.710	0.91
34_L	38_Y	0.709	0.91
153_F	157_K	0.708	0.91
204_T	213_F	0.708	0.91
297_D	304_S	0.708	0.91
129_I	145_V	0.708	0.91
203_Y	207_A	0.707	0.91
90_H	133_L	0.705	0.91
148_Y	151_I	0.705	0.91
71_K	96_I	0.705	0.91
96_I	100_F	0.703	0.91
286_L	331_P	0.702	0.90
29_E	33_E	0.700	0.90
299_L	303_I	0.700	0.90
87_L	90_H	0.700	0.90
141_V	145_V	0.695	0.90
224_L	256_R	0.695	0.90
40_T	44_K	0.694	0.90
230_V	234_K	0.693	0.90
206_D	311_I	0.692	0.90
48_Y	77_L	0.690	0.90
229_E	236_Q	0.688	0.90
41_L	44_K	0.685	0.89
205_L	306_T	0.684	0.89
90_H	93_D	0.683	0.89
23_I	181_L	0.683	0.89
65_I	70_K	0.682	0.89
132_T	135_K	0.682	0.89
222_Y	301_R	0.681	0.89
205_L	269_V	0.681	0.89
234_K	237_S	0.678	0.89
35_I	39_T	0.674	0.89
103_L	107_R	0.674	0.89
309_R	313_R	0.674	0.89
171_E	184_R	0.673	0.89
168_E	186_K	0.669	0.88
89_S	130_K	0.666	0.88
174_I	181_L	0.665	0.88
281_F	315_L	0.664	0.88
234_K	246_G	0.663	0.88
134_R	169_L	0.663	0.88
37_E	41_L	0.661	0.88
44_K	81_L	0.661	0.88
282_A	327_K	0.660	0.88
257_E	305_R	0.659	0.88
189_W	324_S	0.659	0.88
261_V	305_R	0.658	0.87
141_V	144_T	0.657	0.87
216_I	306_T	0.653	0.87
238_F	246_G	0.653	0.87
333_F	344_V	0.648	0.87
99_A	103_L	0.647	0.87
157_K	160_F	0.647	0.87
68_P	74_Y	0.646	0.86
73_V	96_I	0.645	0.86
224_L	228_M	0.643	0.86
349_G	352_F	0.640	0.86
106_W	109_R	0.634	0.85
52_L	77_L	0.632	0.85
20_T	184_R	0.632	0.85
67_L	75_K	0.631	0.85
26_D	154_S	0.631	0.85
75_K	96_I	0.630	0.85
145_V	148_Y	0.629	0.85
56_Q	61_G	0.629	0.85
60_N	64_K	0.628	0.85
189_W	192_P	0.628	0.85
355_E	363_R	0.626	0.85
262_L	266_H	0.626	0.85
274_D	280_F	0.626	0.85
144_T	147_P	0.626	0.85
69_K	75_K	0.625	0.85
38_Y	88_A	0.625	0.85
221_L	224_L	0.624	0.85
292_F	297_D	0.623	0.84
371_I	374_K	0.621	0.84
340_R	361_L	0.620	0.84
137_V	142_R	0.619	0.84
234_K	249_L	0.618	0.84
51_S	122_V	0.617	0.84
52_L	55_I	0.615	0.84
53_T	70_K	0.614	0.84
41_L	84_I	0.613	0.83
149_E	152_T	0.613	0.83
337_S	342_G	0.611	0.83
243_T	250_L	0.610	0.83
70_K	93_D	0.610	0.83
92_V	95_A	0.610	0.83
231_K	235_V	0.609	0.83
127_V	133_L	0.608	0.83
288_K	303_I	0.608	0.83
315_L	326_V	0.608	0.83
55_I	58_R	0.607	0.83
22_S	182_P	0.606	0.83
72_E	77_L	0.605	0.83
317_Y	321_L	0.605	0.83
67_L	70_K	0.605	0.83
224_L	253_Y	0.600	0.82
254_S	261_V	0.599	0.82
67_L	97_N	0.599	0.82
25_N	153_F	0.599	0.82
361_L	366_N	0.598	0.82
203_Y	350_K	0.596	0.82
27_K	32_K	0.595	0.82
276_Y	281_F	0.595	0.82
244_K	248_E	0.593	0.81
21_Y	157_K	0.592	0.81
347_K	362_D	0.592	0.81
312_H	316_K	0.591	0.81
128_R	131_T	0.587	0.81
74_Y	96_I	0.587	0.81
108_K	117_I	0.585	0.81
209_D	365_L	0.584	0.80
126_Y	130_K	0.583	0.80
128_R	173_I	0.583	0.80
71_K	74_Y	0.583	0.80
199_D	335_S	0.582	0.80
126_Y	129_I	0.582	0.80
205_L	265_V	0.580	0.80
375_M	379_P	0.580	0.80
194_N	276_Y	0.580	0.80
330_N	333_F	0.580	0.80
38_Y	87_L	0.579	0.80
345_S	350_K	0.579	0.80
130_K	133_L	0.578	0.80
70_K	77_L	0.578	0.80
337_S	352_F	0.577	0.80
332_R	336_R	0.577	0.80
347_K	351_T	0.576	0.80
314_V	318_K	0.576	0.80
89_S	133_L	0.576	0.80
208_Y	214_V	0.574	0.79
134_R	172_P	0.573	0.79
37_E	87_L	0.572	0.79
96_I	99_A	0.571	0.79
87_L	92_V	0.571	0.79
49_L	73_V	0.569	0.79
233_A	236_Q	0.568	0.79
376_C	379_P	0.567	0.78
51_S	54_K	0.567	0.78
38_Y	129_I	0.567	0.78
153_F	158_S	0.567	0.78
319_A	322_Y	0.567	0.78
34_L	37_E	0.567	0.78
222_Y	302_K	0.565	0.78
30_E	33_E	0.563	0.78
282_A	374_K	0.562	0.78
91_Y	129_I	0.562	0.78
309_R	312_H	0.560	0.78
202_L	293_V	0.560	0.78
288_K	291_M	0.559	0.78
349_G	355_E	0.559	0.78
95_A	99_A	0.553	0.77
60_N	63_Y	0.552	0.77
214_V	271_A	0.551	0.77
91_Y	127_V	0.551	0.77
259_N	262_L	0.550	0.76
335_S	345_S	0.545	0.76
37_E	40_T	0.545	0.76
333_F	352_F	0.544	0.76
214_V	268_F	0.544	0.76
74_Y	93_D	0.544	0.76
137_V	144_T	0.542	0.75
197_A	282_A	0.541	0.75
62_N	65_I	0.541	0.75
267_K	270_N	0.540	0.75
39_T	42_L	0.540	0.75
218_L	306_T	0.536	0.75
52_L	56_Q	0.536	0.75
69_K	76_P	0.536	0.75
180_Y	255_H	0.536	0.75
373_L	376_C	0.535	0.74
243_T	246_G	0.535	0.74
268_F	311_I	0.533	0.74
95_A	98_D	0.532	0.74
203_Y	346_R	0.531	0.74
178_K	259_N	0.531	0.74
342_G	370_N	0.530	0.74
194_N	277_P	0.523	0.73
373_L	378_F	0.522	0.73
64_K	69_K	0.522	0.73
254_S	258_R	0.520	0.72
65_I	68_P	0.520	0.72
73_V	76_P	0.519	0.72
129_I	133_L	0.519	0.72
229_E	297_D	0.517	0.72
296_N	303_I	0.516	0.72
222_Y	225_K	0.516	0.72
108_K	111_I	0.515	0.72
221_L	225_K	0.515	0.72
282_A	376_C	0.514	0.71
225_K	229_E	0.511	0.71
353_E	359_F	0.509	0.71
39_T	127_V	0.508	0.71
299_L	302_K	0.508	0.71
25_N	174_I	0.505	0.70
48_Y	83_K	0.505	0.70
262_L	307_V	0.505	0.70
185_Y	274_D	0.504	0.70
345_S	348_V	0.502	0.70
290_S	293_V	0.501	0.70
229_E	237_S	0.500	0.69

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1nu0A	1	0.3073	95.1	0.933	Contact Map
1vhxA	2	0.3239	94.5	0.936	Contact Map
1iv0A	1	0.2293	91.4	0.942	Contact Map
2k4xA	1	0.1253	84.4	0.949	Contact Map
1qypA	1	0.1017	84.3	0.949	Contact Map
4aybP	1	0.0946	83.3	0.949	Contact Map
3j20Y	1	0.1135	81.6	0.95	Contact Map
1ffkW	1	0.1655	77.3	0.952	Contact Map
1vd4A	1	0.1253	72.4	0.954	Contact Map
3j3w0	1	0.13	67.9	0.956	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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