Q97YH3 - SULSO - GREMLIN Contacts

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Q97YH3 - Second ORF in transposon ISC1904

UniProt:	Q97YH3	Sequences: MHFTYSIKNSEREKSKELIKEYRTLLQKAIDYLWNLTKIQVRKKNGNYKITLPKKKEVYKPLREELEKINHLASHYVDKAINDAFSILKSWRKRAIKGRASIEKPRVKKAYVRIKTTLRKVVGESVRITVRPHEYITFSWSKSWFSRRVKELELGEPIIKEEKVYLPFRYKLPWVTPVNFLAIDSNLYTLDAYDGEKFVTISLKQLYSLKYSMEVKRAKVQSFASKHTKRERVDEEVFA	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	239 (214)
Sequences:	147 (70)
Seq/√Len:	4.8

SULSO - Q97YH3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
59_Y	72_L	2.837	0.64
78_D	115_K	2.622	0.57
75_H	118_L	2.535	0.55
229_K	236_E	2.344	0.49
229_K	235_E	2.325	0.48
225_S	234_D	2.300	0.47
131_R	134_E	2.281	0.47
63_R	196_E	2.252	0.46
81_I	115_K	2.234	0.45
153_E	229_K	2.140	0.42
134_E	152_L	2.136	0.42
73_A	129_T	2.068	0.40
15_S	126_V	1.952	0.37
234_D	237_V	1.936	0.36
75_H	115_K	1.883	0.35
97_K	176_T	1.876	0.34
73_A	187_L	1.870	0.34
22_Y	26_L	1.795	0.32
78_D	81_I	1.736	0.30
76_Y	118_L	1.706	0.30
133_H	232_R	1.696	0.29
30_I	183_I	1.672	0.29
139_S	157_P	1.658	0.28
163_K	189_T	1.649	0.28
91_W	203_L	1.646	0.28
30_I	92_R	1.643	0.28
90_S	123_G	1.619	0.27
147_R	160_K	1.619	0.27
136_I	164_V	1.603	0.27
147_R	153_E	1.589	0.27
220_V	234_D	1.586	0.27
76_Y	82_N	1.584	0.27
59_Y	73_A	1.581	0.26
36_L	183_I	1.578	0.26
55_K	81_I	1.552	0.26
89_K	170_Y	1.551	0.26
17_E	147_R	1.534	0.25
37_T	153_E	1.526	0.25
27_Q	30_I	1.522	0.25
75_H	81_I	1.520	0.25
73_A	134_E	1.516	0.25
98_G	126_V	1.485	0.24
229_K	234_D	1.467	0.24
217_R	220_V	1.465	0.24
96_I	222_S	1.462	0.24
13_E	97_K	1.460	0.24
125_S	232_R	1.448	0.23
87_I	156_E	1.443	0.23
59_Y	74_S	1.442	0.23
109_K	222_S	1.438	0.23
166_L	201_I	1.431	0.23
79_K	116_T	1.417	0.23
104_K	126_V	1.400	0.22
219_K	229_K	1.395	0.22
132_P	155_G	1.384	0.22
132_P	167_P	1.383	0.22
63_R	219_K	1.362	0.21
20_K	24_T	1.355	0.21
131_R	152_L	1.350	0.21
215_V	219_K	1.348	0.21
89_K	190_L	1.339	0.21
33_L	115_K	1.336	0.21
76_Y	80_A	1.327	0.21
223_F	231_E	1.324	0.21
127_R	173_P	1.322	0.21
99_R	185_S	1.312	0.20
76_Y	79_K	1.306	0.20
166_L	226_K	1.305	0.20
146_S	182_A	1.304	0.20
89_K	163_K	1.298	0.20
128_I	138_F	1.296	0.20
73_A	131_R	1.293	0.20
82_N	116_T	1.289	0.20
75_H	78_D	1.288	0.20
29_A	54_K	1.276	0.20
14_K	94_R	1.269	0.20
92_R	166_L	1.258	0.19
138_F	141_S	1.252	0.19
91_W	119_R	1.243	0.19
221_Q	234_D	1.241	0.19
87_I	92_R	1.238	0.19
86_S	127_R	1.236	0.19
91_W	216_K	1.232	0.19
120_K	137_T	1.230	0.19
129_T	187_L	1.210	0.18
98_G	177_P	1.208	0.18
166_L	222_S	1.200	0.18
13_E	199_V	1.181	0.18
30_I	180_F	1.180	0.18
140_W	173_P	1.179	0.18
94_R	156_E	1.170	0.18
172_L	190_L	1.168	0.18
138_F	192_A	1.158	0.17
190_L	220_V	1.155	0.17
197_K	209_L	1.154	0.17
10_S	133_H	1.152	0.17
30_I	87_I	1.149	0.17
7_I	169_R	1.147	0.17
99_R	236_E	1.139	0.17
53_P	229_K	1.130	0.17
92_R	183_I	1.126	0.17
106_R	128_I	1.119	0.17
91_W	95_A	1.118	0.17
54_K	59_Y	1.101	0.16
63_R	172_L	1.092	0.16
55_K	76_Y	1.088	0.16
123_G	166_L	1.087	0.16
34_W	181_L	1.087	0.16
136_I	233_V	1.086	0.16
93_K	154_L	1.083	0.16
127_R	176_T	1.079	0.16
189_T	213_M	1.075	0.16
78_D	116_T	1.070	0.16
27_Q	222_S	1.062	0.16
13_E	31_D	1.058	0.16
107_V	215_V	1.053	0.16
118_L	221_Q	1.048	0.15
195_G	214_E	1.042	0.15
78_D	117_T	1.042	0.15
11_E	14_K	1.037	0.15
35_N	57_E	1.020	0.15
188_Y	191_D	1.018	0.15
63_R	164_V	1.017	0.15
192_A	201_I	1.011	0.15
94_R	126_V	1.005	0.15
159_I	233_V	1.002	0.15
40_Q	89_K	0.994	0.15
8_K	11_E	0.993	0.15
8_K	14_K	0.993	0.15
25_L	36_L	0.991	0.15
63_R	229_K	0.984	0.14
7_I	11_E	0.977	0.14
58_V	82_N	0.971	0.14
55_K	74_S	0.969	0.14
97_K	159_I	0.964	0.14
7_I	10_S	0.958	0.14
150_K	181_L	0.952	0.14
34_W	85_F	0.952	0.14
55_K	75_H	0.950	0.14
227_H	230_R	0.950	0.14
35_N	125_S	0.949	0.14
79_K	210_K	0.930	0.14
109_K	196_E	0.925	0.14
78_D	118_L	0.925	0.14
81_I	114_I	0.924	0.14
29_A	33_L	0.924	0.14
29_A	183_I	0.922	0.14
99_R	162_E	0.920	0.13
219_K	222_S	0.911	0.13
180_F	185_S	0.910	0.13
7_I	162_E	0.899	0.13
30_I	94_R	0.896	0.13
63_R	222_S	0.896	0.13
163_K	232_R	0.891	0.13
153_E	178_V	0.890	0.13
109_K	221_Q	0.887	0.13
182_A	195_G	0.884	0.13
15_S	111_Y	0.882	0.13
139_S	145_F	0.880	0.13
41_V	117_T	0.877	0.13
64_E	167_P	0.875	0.13
140_W	157_P	0.868	0.13
168_F	206_L	0.866	0.13
78_D	135_Y	0.858	0.13
79_K	115_K	0.857	0.13
75_H	80_A	0.852	0.13
39_I	96_I	0.852	0.13
120_K	164_V	0.850	0.12
27_Q	94_R	0.846	0.12
92_R	154_L	0.845	0.12
119_R	210_K	0.845	0.12
18_L	120_K	0.841	0.12
66_L	72_L	0.841	0.12
99_R	144_W	0.835	0.12
138_F	190_L	0.835	0.12
147_R	225_S	0.833	0.12
97_K	104_K	0.831	0.12
151_E	222_S	0.830	0.12
90_S	162_E	0.825	0.12
151_E	165_Y	0.817	0.12
154_L	199_V	0.817	0.12
228_T	231_E	0.816	0.12
145_F	223_F	0.810	0.12
11_E	139_S	0.809	0.12
101_S	112_V	0.806	0.12
59_Y	83_D	0.805	0.12
73_A	152_L	0.805	0.12
31_D	34_W	0.803	0.12
31_D	97_K	0.799	0.12
65_E	233_V	0.791	0.12
99_R	135_Y	0.791	0.12
79_K	82_N	0.789	0.12
79_K	117_T	0.788	0.12
181_L	227_H	0.786	0.12
138_F	194_D	0.784	0.12
126_V	192_A	0.782	0.12
153_E	171_K	0.777	0.12
63_R	149_V	0.775	0.11
113_R	155_G	0.771	0.11
132_P	144_W	0.771	0.11
30_I	89_K	0.758	0.11
76_Y	81_I	0.757	0.11
30_I	216_K	0.756	0.11
119_R	216_K	0.756	0.11
155_G	214_E	0.755	0.11
22_Y	107_V	0.754	0.11
113_R	156_E	0.752	0.11
16_K	20_K	0.750	0.11
23_R	232_R	0.748	0.11
59_Y	152_L	0.743	0.11
109_K	214_E	0.742	0.11
86_S	99_R	0.741	0.11
58_V	117_T	0.741	0.11
87_I	94_R	0.737	0.11
16_K	112_V	0.737	0.11
173_P	189_T	0.737	0.11
39_I	129_T	0.736	0.11
20_K	34_W	0.734	0.11
66_L	210_K	0.725	0.11
160_K	209_L	0.724	0.11
106_R	162_E	0.722	0.11
73_A	79_K	0.721	0.11
153_E	190_L	0.718	0.11
35_N	65_E	0.715	0.11
93_K	124_E	0.708	0.11
138_F	201_I	0.708	0.11
105_P	186_N	0.701	0.11
105_P	184_D	0.701	0.11
17_E	151_E	0.700	0.11
128_I	169_R	0.698	0.11
214_E	220_V	0.695	0.11
83_D	87_I	0.693	0.10
128_I	159_I	0.693	0.10
138_F	169_R	0.685	0.10
157_P	173_P	0.675	0.10
75_H	79_K	0.672	0.10
127_R	145_F	0.671	0.10
119_R	148_R	0.665	0.10
56_K	175_V	0.664	0.10
75_H	127_R	0.664	0.10
80_A	237_V	0.663	0.10
55_K	59_Y	0.662	0.10
191_D	201_I	0.662	0.10
173_P	225_S	0.662	0.10
108_K	191_D	0.658	0.10
109_K	174_W	0.652	0.10
170_Y	213_M	0.646	0.10
54_K	115_K	0.645	0.10
55_K	79_K	0.644	0.10
42_R	175_V	0.641	0.10
84_A	100_A	0.638	0.10
73_A	203_L	0.637	0.10
80_A	209_L	0.635	0.10
88_L	113_R	0.634	0.10
17_E	90_S	0.624	0.10
207_Y	214_E	0.621	0.10
62_L	216_K	0.620	0.10
100_A	177_P	0.620	0.10
80_A	129_T	0.619	0.10
178_V	181_L	0.618	0.10
89_K	189_T	0.617	0.10
7_I	164_V	0.616	0.10
128_I	182_A	0.615	0.10
60_K	129_T	0.614	0.10
71_H	220_V	0.613	0.10
93_K	236_E	0.613	0.10
118_L	127_R	0.612	0.10
55_K	212_S	0.611	0.10
98_G	170_Y	0.611	0.10
160_K	172_L	0.610	0.10
41_V	55_K	0.609	0.10
191_D	210_K	0.609	0.10
63_R	235_E	0.609	0.10
26_L	107_V	0.607	0.09
190_L	217_R	0.605	0.09
88_L	149_V	0.603	0.09
118_L	129_T	0.603	0.09
133_H	233_V	0.602	0.09
41_V	116_T	0.600	0.09
160_K	166_L	0.600	0.09
147_R	220_V	0.594	0.09
7_I	26_L	0.594	0.09
182_A	215_V	0.592	0.09
124_E	166_L	0.591	0.09
59_Y	96_I	0.589	0.09
67_E	189_T	0.588	0.09
17_E	154_L	0.584	0.09
53_P	113_R	0.581	0.09
120_K	155_G	0.578	0.09
54_K	112_V	0.576	0.09
82_N	111_Y	0.574	0.09
84_A	92_R	0.573	0.09
134_E	170_Y	0.568	0.09
63_R	216_K	0.564	0.09
157_P	161_E	0.564	0.09
75_H	116_T	0.562	0.09
20_K	88_L	0.560	0.09
123_G	181_L	0.554	0.09
190_L	237_V	0.548	0.09
182_A	201_I	0.545	0.09
79_K	114_I	0.543	0.09
103_E	162_E	0.540	0.09
30_I	42_R	0.540	0.09
63_R	74_S	0.537	0.09
11_E	140_W	0.537	0.09
147_R	234_D	0.533	0.09
53_P	191_D	0.529	0.09
125_S	154_L	0.526	0.09
63_R	212_S	0.524	0.09
136_I	160_K	0.523	0.09
123_G	154_L	0.522	0.09
88_L	159_I	0.521	0.09
129_T	135_Y	0.519	0.09
32_Y	79_K	0.517	0.09
168_F	190_L	0.516	0.09
120_K	193_Y	0.514	0.09
110_A	151_E	0.510	0.08
76_Y	213_M	0.508	0.08
94_R	119_R	0.505	0.08
161_E	233_V	0.502	0.08
83_D	107_V	0.500	0.08

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2bw2A	1	0.523	22.6	0.962	Contact Map
3e9vA	1	0.431	7.7	0.969	Contact Map
4oh8B	1	0.1757	7.2	0.969	Contact Map
2e5yA	2	0.5146	7.1	0.969	Contact Map
3fxdA	1	0.205	6.6	0.97	Contact Map
4fkeA	1	0.2343	5.5	0.971	Contact Map
2qe7H	1	0.5146	5.5	0.971	Contact Map
4lvqA	1	0.2176	4.4	0.972	Contact Map
4i2oA	2	0.251	4.3	0.972	Contact Map
2rq6A	1	0.5105	4.2	0.973	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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