GREMLIN Database
CAS1A - CRISPR-associated endonuclease Cas1 1
UniProt: Q97YC1 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 352 (294)
Sequences: 2019 (1587)
Seq/√Len: 92.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
249_G296_V5.8931.00
164_V210_A4.9781.00
146_T149_K3.7881.00
194_I203_V3.7111.00
68_K77_K3.0331.00
162_G348_P2.8381.00
143_K146_T2.8351.00
249_G300_L2.8091.00
107_V117_I2.6271.00
254_R288_M2.6031.00
142_L276_F2.4381.00
232_M239_L2.4361.00
88_P115_I2.4271.00
141_W267_N2.3621.00
201_E204_M2.3571.00
147_A340_I2.3051.00
109_V113_Y2.3041.00
257_V329_I2.2641.00
236_S247_N2.2411.00
74_I106_V2.2301.00
111_N128_K2.2211.00
253_L296_V2.1961.00
158_A348_P2.1421.00
164_V194_I2.1071.00
88_P109_V2.1001.00
95_V120_F2.0301.00
245_A304_A2.0211.00
280_R285_F2.0011.00
205_Q208_A1.9901.00
257_V288_M1.9881.00
67_L106_V1.9851.00
96_V100_S1.9481.00
69_V109_V1.9301.00
198_N202_E1.9301.00
66_Y101_S1.8871.00
323_D331_T1.8831.00
147_A151_R1.8601.00
199_T202_E1.8161.00
173_Y298_R1.7921.00
155_F159_F1.7891.00
242_F300_L1.7741.00
138_F270_I1.7421.00
73_L85_S1.7021.00
186_E213_V1.6981.00
153_V157_K1.6681.00
235_V247_N1.6561.00
245_A309_D1.6481.00
142_L148_W1.6411.00
245_A300_L1.6391.00
66_Y103_S1.6321.00
94_I110_A1.6211.00
120_F263_S1.6131.00
106_V110_A1.5971.00
158_A345_E1.5811.00
164_V187_L1.5671.00
241_P316_T1.5601.00
153_V203_V1.5561.00
200_A204_M1.5461.00
153_V197_V1.5451.00
58_A94_I1.5401.00
167_Q211_A1.5221.00
261_V284_V1.5161.00
143_K147_A1.5041.00
74_I88_P1.4991.00
190_L213_V1.4901.00
225_S311_V1.4881.00
287_L333_A1.4861.00
242_F304_A1.4761.00
78_I83_K1.4751.00
214_Y297_D1.4601.00
59_F97_L1.4441.00
62_D78_I1.4370.99
75_T85_S1.4330.99
70_E85_S1.4270.99
74_I110_A1.4220.99
290_E332_Q1.4200.99
74_I94_I1.4140.99
183_T220_S1.3940.99
168_W187_L1.3860.99
183_T217_G1.3670.99
241_P311_V1.3640.99
263_S330_Y1.3560.99
65_A76_C1.3480.99
167_Q293_S1.3430.99
65_A78_I1.3410.99
258_W284_V1.3310.99
154_E345_E1.3280.99
261_V333_A1.3150.99
164_V191_A1.2830.99
260_A333_A1.2650.99
200_A275_K1.2590.99
219_K240_D1.2540.99
204_M273_L1.2520.99
172_R184_S1.2420.98
99_N105_E1.2380.98
291_F328_E1.2360.98
64_G100_S1.2290.98
183_T213_V1.2280.98
58_A91_L1.2230.98
82_V85_S1.2210.98
254_R285_F1.2180.98
174_Y221_L1.2170.98
261_V287_L1.2130.98
141_W145_L1.2100.98
163_K297_D1.2050.98
153_V199_T1.2000.98
168_W188_D1.1990.98
160_I273_L1.1980.98
212_K216_R1.1860.98
226_L304_A1.1790.98
219_K227_G1.1740.98
302_G308_A1.1730.98
209_E212_K1.1440.97
63_Y99_N1.1430.97
110_A117_I1.1410.97
161_Y195_T1.1400.97
314_L321_F1.1320.97
189_R193_E1.1320.97
168_W184_S1.1290.97
256_V326_E1.1190.97
78_I81_Q1.1190.97
94_I117_I1.1020.97
169_V172_R1.0970.97
72_G113_Y1.0930.97
296_V300_L1.0910.97
165_H350_L1.0880.96
57_I95_V1.0870.96
88_P113_Y1.0840.96
63_Y98_T1.0830.96
168_W191_A1.0800.96
288_M292_R1.0800.96
170_T293_S1.0760.96
159_F290_E1.0760.96
253_L288_M1.0760.96
152_K275_K1.0750.96
151_R154_E1.0750.96
142_L149_K1.0710.96
250_Y297_D1.0650.96
172_R188_D1.0620.96
159_F162_G1.0570.96
190_L206_K1.0550.96
88_P110_A1.0470.96
156_A200_A1.0410.95
187_L210_A1.0370.95
121_N125_P1.0280.95
69_V88_P1.0250.95
218_V242_F1.0250.95
160_I203_V1.0230.95
161_Y194_I1.0170.95
155_F158_A1.0120.95
222_L242_F1.0110.95
290_E346_Y0.9980.94
272_F336_L0.9970.94
93_S116_E0.9930.94
249_G299_K0.9930.94
281_I285_F0.9900.94
58_A74_I0.9890.94
186_E189_R0.9880.94
310_K313_D0.9840.94
118_V255_K0.9770.94
264_V333_A0.9730.93
208_A289_E0.9710.93
127_A131_P0.9640.93
211_A297_D0.9630.93
190_L209_E0.9600.93
142_L145_L0.9560.93
246_L300_L0.9500.93
102_I106_V0.9490.93
160_I194_I0.9490.93
77_K82_V0.9470.93
63_Y122_K0.9440.92
155_F336_L0.9380.92
138_F145_L0.9370.92
168_W172_R0.9370.92
206_K209_E0.9340.92
330_Y334_R0.9310.92
213_V216_R0.9220.92
157_K197_V0.9190.91
258_W281_I0.9140.91
74_I115_I0.9140.91
254_R281_I0.9060.91
170_T174_Y0.9050.91
235_V244_R0.8940.90
165_H191_A0.8830.90
75_T82_V0.8820.90
175_E184_S0.8810.90
209_E213_V0.8790.89
163_K211_A0.8760.89
170_T301_I0.8760.89
246_L250_Y0.8710.89
190_L210_A0.8700.89
153_V194_I0.8650.89
294_P325_K0.8620.89
163_K265_G0.8560.88
139_K143_K0.8560.88
102_I107_V0.8500.88
157_K194_I0.8480.88
171_L217_G0.8460.88
68_K103_S0.8430.87
156_A273_L0.8430.87
163_K292_R0.8380.87
171_L184_S0.8330.87
309_D313_D0.8300.87
151_R339_A0.8280.86
314_L318_Y0.8250.86
108_K127_A0.8250.86
254_R258_W0.8200.86
185_Q188_D0.8200.86
339_A342_N0.8170.86
175_E179_G0.8100.85
68_K75_T0.8090.85
57_I93_S0.8060.85
204_M275_K0.8050.85
154_E157_K0.8040.85
328_E349_Y0.7990.84
222_L304_A0.7970.84
148_W152_K0.7960.84
215_W250_Y0.7950.84
260_A264_V0.7870.84
155_F339_A0.7870.84
234_R285_F0.7870.84
158_A161_Y0.7850.83
93_S118_V0.7840.83
107_V119_F0.7820.83
233_K247_N0.7740.83
141_W278_S0.7740.83
136_G277_R0.7720.82
140_V340_I0.7710.82
161_Y191_A0.7690.82
171_L214_Y0.7680.82
72_G109_V0.7660.82
142_L275_K0.7660.82
232_M240_D0.7620.82
163_K250_Y0.7600.81
269_Y281_I0.7570.81
337_V341_L0.7540.81
96_V119_F0.7530.81
183_T216_R0.7530.81
289_E292_R0.7520.81
171_L183_T0.7510.81
143_K341_L0.7490.80
194_I197_V0.7460.80
150_R196_F0.7460.80
74_I91_L0.7440.80
101_S105_E0.7440.80
136_G278_S0.7430.80
233_K236_S0.7420.80
173_Y301_I0.7410.80
56_K90_E0.7400.80
89_T113_Y0.7400.80
282_S285_F0.7390.80
138_F142_L0.7380.79
140_V278_S0.7370.79
56_K76_C0.7370.79
325_K328_E0.7330.79
154_E158_A0.7300.79
69_V106_V0.7300.79
264_V334_R0.7290.79
150_R154_E0.7260.78
240_D318_Y0.7260.78
197_V202_E0.7240.78
136_G267_N0.7230.78
158_A162_G0.7210.78
302_G306_E0.7210.78
253_L257_V0.7180.78
191_A194_I0.7150.77
333_A337_V0.7110.77
157_K161_Y0.7090.77
112_E132_A0.7080.77
264_V330_Y0.7070.77
172_R176_R0.7060.76
314_L319_S0.7040.76
174_Y301_I0.7030.76
327_D330_Y0.7030.76
336_L340_I0.7010.76
94_I115_I0.6990.76
105_E108_K0.6990.76
159_F272_F0.6980.76
108_K112_E0.6970.75
106_V109_V0.6970.75
152_K200_A0.6960.75
257_V287_L0.6960.75
153_V275_K0.6930.75
206_K213_V0.6930.75
257_V333_A0.6900.75
314_L317_V0.6840.74
167_Q292_R0.6830.74
257_V260_A0.6820.74
140_V341_L0.6800.74
143_K151_R0.6760.73
173_Y176_R0.6750.73
159_F273_L0.6720.73
201_E205_Q0.6700.73
251_G255_K0.6690.72
241_P314_L0.6640.72
114_G264_V0.6630.72
186_E209_E0.6580.71
197_V206_K0.6560.71
63_Y105_E0.6540.71
88_P106_V0.6540.71
249_G253_L0.6530.71
270_I276_F0.6520.71
197_V203_V0.6500.70
118_V129_L0.6490.70
94_I224_K0.6490.70
170_T298_R0.6470.70
115_I213_V0.6450.70
192_R195_T0.6350.69
165_H169_V0.6340.68
112_E240_D0.6340.68
248_I251_G0.6320.68
246_L292_R0.6300.68
148_W275_K0.6290.68
107_V111_N0.6290.68
226_L244_R0.6290.68
208_A314_L0.6280.68
138_F276_F0.6260.67
295_F325_K0.6250.67
116_E128_K0.6240.67
130_I281_I0.6240.67
313_D318_Y0.6230.67
79_K213_V0.6230.67
183_T188_D0.6190.67
285_F288_M0.6180.66
66_Y110_A0.6140.66
223_P227_G0.6140.66
158_A350_L0.6130.66
260_A263_S0.6090.65
81_Q84_W0.6090.65
180_Y202_E0.6080.65
287_L340_I0.6080.65
79_K82_V0.6070.65
232_M236_S0.6060.65
203_V227_G0.6060.65
137_S280_R0.6050.65
338_N343_D0.6050.65
230_G242_F0.6030.65
265_G337_V0.6020.64
271_G276_F0.6010.64
74_I256_V0.5990.64
129_L262_I0.5980.64
299_K303_L0.5960.64
219_K229_K0.5960.64
251_G291_F0.5950.64
226_L245_A0.5940.63
86_I94_I0.5940.63
194_I210_A0.5930.63
127_A130_I0.5920.63
324_V327_D0.5910.63
262_I268_P0.5910.63
202_E206_K0.5900.63
86_I229_K0.5880.63
317_V320_L0.5870.63
90_E216_R0.5860.62
159_F346_Y0.5860.62
274_H289_E0.5840.62
156_A204_M0.5810.62
63_Y100_S0.5800.62
264_V337_V0.5790.61
232_M241_P0.5780.61
145_L270_I0.5730.61
193_E224_K0.5730.61
147_A339_A0.5710.60
141_W269_Y0.5700.60
185_Q189_R0.5700.60
298_R305_R0.5690.60
130_I135_A0.5680.60
98_T314_L0.5660.60
236_S314_L0.5630.59
298_R303_L0.5620.59
317_V321_F0.5620.59
291_F333_A0.5610.59
161_Y203_V0.5600.59
163_K167_Q0.5600.59
163_K289_E0.5560.58
205_Q242_F0.5560.58
222_L306_E0.5550.58
191_A195_T0.5550.58
128_K131_P0.5540.58
57_I252_M0.5510.58
90_E113_Y0.5500.58
323_D328_E0.5500.58
194_I206_K0.5500.58
260_A330_Y0.5500.58
156_A283_L0.5490.57
157_K195_T0.5480.57
60_V65_A0.5470.57
230_G240_D0.5470.57
189_R197_V0.5460.57
217_G220_S0.5460.57
267_N271_G0.5460.57
207_E274_H0.5450.57
111_N127_A0.5450.57
248_I255_K0.5440.57
70_E75_T0.5430.57
155_F272_F0.5430.57
332_Q346_Y0.5430.57
173_Y233_K0.5420.56
211_A292_R0.5420.56
225_S241_P0.5400.56
140_V280_R0.5380.56
269_Y276_F0.5360.56
323_D327_D0.5360.56
141_W279_G0.5360.56
116_E259_G0.5350.56
110_A115_I0.5350.56
150_R202_E0.5330.55
339_A343_D0.5310.55
121_N128_K0.5310.55
218_V226_L0.5300.55
188_D192_R0.5250.54
103_S163_K0.5230.54
265_G292_R0.5210.54
104_S119_F0.5210.54
267_N278_S0.5210.54
247_N285_F0.5200.53
311_V315_K0.5180.53
157_K203_V0.5180.53
266_L283_L0.5180.53
102_I119_F0.5160.53
211_A250_Y0.5150.53
172_R325_K0.5150.53
103_S265_G0.5150.53
98_T313_D0.5130.52
304_A309_D0.5130.52
139_K277_R0.5130.52
102_I117_I0.5130.52
235_V314_L0.5120.52
137_S141_W0.5100.52
141_W280_R0.5080.52
143_K340_I0.5080.52
148_W270_I0.5070.52
233_K320_L0.5070.52
195_T199_T0.5060.51
166_N298_R0.5050.51
309_D314_L0.5040.51
171_L310_K0.5040.51
100_S104_S0.5020.51
145_L267_N0.5010.51
256_V329_I0.5000.51
159_F297_D0.5000.51
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4n06A 2 0.8438 100 0.249 Contact Map
4wj0A 2 0.8097 100 0.261 Contact Map
4xtkA 2 0.8324 100 0.269 Contact Map
2yzsA 4 0.7898 100 0.282 Contact Map
4w8kA 2 0.821 100 0.367 Contact Map
3godA 4 0.8438 100 0.433 Contact Map
3nkdA 2 0.7159 100 0.46 Contact Map
3nkeA 3 0.5028 100 0.614 Contact Map
4ujqJ 1 0.5455 20.1 0.969 Contact Map
3zf7x 1 0.5511 11.4 0.972 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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