Q97YC0 - SULSO - GREMLIN Contacts

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Q97YC0 - Uncharacterized protein

UniProt:	Q97YC0	Sequences: MIYTLTFRLKPSNDVIIPPFSSKLSRTLFLSFSPTYSKIIESKEPNKPLRITVVKDQGKPLYSNGKSKVVLKAENTYTFIVNTLLEDVVKEVIRVESVNREIYNTSFHVELVNVSVKENVMAEDARFYRVHFKTPTLLQPPRPRMKRKENRYVLFPYVPLLFYSIASHWNRYMDKKIVGVTGSKTLYYFREVNYRIRPMTAYYGNIPNKGFVGWVVFELSARKGSKIRENIRRLLDYVNYFGVGKSRNIGFGEVEVKSLNG	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	261 (228)
Sequences:	341 (293)
Seq/√Len:	19.4

SULSO - Q97YC0 CAS6B CAS6A Q97YE5 Q97YB0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
50_R	82_N	5.326	1.00
161_L	217_F	4.682	1.00
165_I	243_V	3.930	1.00
243_V	254_V	3.468	1.00
144_R	245_K	2.973	0.99
132_F	136_T	2.872	0.99
26_R	49_L	2.850	0.99
238_V	254_V	2.830	0.99
7_F	81_V	2.806	0.99
41_E	138_L	2.786	0.99
14_D	73_A	2.657	0.98
25_S	79_F	2.433	0.97
237_Y	241_F	2.425	0.96
56_D	77_Y	2.292	0.95
165_I	241_F	2.290	0.95
27_T	41_E	2.264	0.95
244_G	252_G	2.173	0.93
9_L	54_V	2.171	0.93
194_Y	211_F	2.167	0.93
168_H	247_R	2.154	0.93
26_R	41_E	2.142	0.93
132_F	164_S	2.127	0.93
7_F	50_R	2.094	0.92
26_R	247_R	2.052	0.91
247_R	251_F	2.041	0.91
246_S	249_I	2.032	0.91
74_E	104_N	1.950	0.89
246_S	251_F	1.941	0.89
244_G	250_G	1.859	0.86
197_R	205_N	1.801	0.85
17_I	229_E	1.753	0.83
129_R	216_V	1.725	0.82
92_V	168_H	1.655	0.79
24_L	134_T	1.655	0.79
28_L	47_K	1.645	0.79
54_V	74_E	1.626	0.78
22_S	53_V	1.597	0.77
82_N	253_E	1.546	0.74
30_L	54_V	1.533	0.74
136_T	211_F	1.526	0.73
77_Y	85_L	1.511	0.72
41_E	164_S	1.468	0.70
80_I	217_F	1.466	0.70
110_E	181_T	1.466	0.70
160_L	217_F	1.462	0.70
138_L	246_S	1.457	0.70
242_G	246_S	1.452	0.69
83_T	197_R	1.442	0.69
213_G	252_G	1.419	0.67
213_G	244_G	1.419	0.67
26_R	246_S	1.417	0.67
37_S	219_L	1.413	0.67
239_N	243_V	1.395	0.66
159_P	163_Y	1.395	0.66
101_E	107_F	1.395	0.66
47_K	251_F	1.390	0.66
233_R	254_V	1.380	0.65
82_N	238_V	1.347	0.63
154_L	158_V	1.332	0.62
130_V	235_L	1.329	0.62
26_R	138_L	1.319	0.62
11_P	14_D	1.305	0.61
21_S	26_R	1.301	0.61
242_G	252_G	1.279	0.59
192_V	218_E	1.273	0.59
108_H	117_K	1.247	0.57
137_L	251_F	1.243	0.57
83_T	89_V	1.241	0.57
165_I	237_Y	1.230	0.56
111_L	114_V	1.212	0.55
201_A	251_F	1.212	0.55
137_L	246_S	1.211	0.55
154_L	194_Y	1.208	0.55
155_F	194_Y	1.197	0.54
160_L	241_F	1.188	0.53
52_T	253_E	1.174	0.53
33_S	92_V	1.172	0.52
227_I	231_I	1.166	0.52
34_P	37_S	1.163	0.52
160_L	237_Y	1.162	0.52
202_Y	207_P	1.161	0.52
73_A	201_A	1.158	0.52
82_N	255_E	1.141	0.50
127_F	216_V	1.139	0.50
17_I	243_V	1.136	0.50
16_I	98_V	1.133	0.50
242_G	247_R	1.112	0.49
81_V	98_V	1.104	0.48
154_L	209_K	1.103	0.48
213_G	250_G	1.103	0.48
56_D	173_M	1.100	0.48
245_K	249_I	1.097	0.48
137_L	154_L	1.093	0.47
84_L	241_F	1.081	0.47
234_L	237_Y	1.072	0.46
110_E	240_Y	1.070	0.46
170_N	234_L	1.069	0.46
7_F	79_F	1.065	0.46
138_L	164_S	1.064	0.46
47_K	248_N	1.052	0.45
18_P	23_K	1.048	0.45
26_R	249_I	1.048	0.45
170_N	210_G	1.035	0.44
7_F	10_K	1.032	0.44
11_P	175_K	1.020	0.43
57_Q	129_R	1.015	0.42
111_L	240_Y	1.009	0.42
139_Q	219_L	1.006	0.42
238_V	243_V	0.996	0.41
228_R	232_R	0.991	0.41
26_R	242_G	0.989	0.41
7_F	57_Q	0.987	0.41
109_V	172_Y	0.987	0.41
8_R	133_K	0.976	0.40
238_V	247_R	0.973	0.40
52_T	238_V	0.970	0.40
233_R	248_N	0.968	0.40
134_T	221_A	0.966	0.39
16_I	19_P	0.961	0.39
137_L	245_K	0.949	0.38
145_M	157_Y	0.948	0.38
142_R	180_V	0.947	0.38
165_I	234_L	0.947	0.38
144_R	251_F	0.946	0.38
33_S	36_Y	0.944	0.38
134_T	208_N	0.943	0.38
41_E	198_P	0.941	0.38
23_K	26_R	0.941	0.38
31_S	218_E	0.937	0.38
103_Y	117_K	0.935	0.38
40_I	49_L	0.929	0.37
45_P	51_I	0.928	0.37
128_Y	232_R	0.926	0.37
27_T	138_L	0.925	0.37
30_L	34_P	0.925	0.37
26_R	169_W	0.921	0.37
170_N	182_G	0.918	0.37
169_W	234_L	0.917	0.36
86_E	90_K	0.913	0.36
160_L	165_I	0.909	0.36
241_F	254_V	0.905	0.36
164_S	243_V	0.904	0.36
86_E	115_S	0.904	0.36
130_V	238_V	0.903	0.36
100_R	119_N	0.902	0.36
42_S	154_L	0.897	0.35
48_P	205_N	0.891	0.35
78_T	128_Y	0.882	0.34
29_F	248_N	0.881	0.34
26_R	139_Q	0.880	0.34
8_R	11_P	0.877	0.34
38_K	142_R	0.876	0.34
145_M	160_L	0.870	0.34
128_Y	235_L	0.869	0.34
108_H	114_V	0.869	0.34
25_S	208_N	0.850	0.33
104_N	122_A	0.845	0.32
14_D	17_I	0.844	0.32
163_Y	183_S	0.842	0.32
10_K	14_D	0.840	0.32
242_G	250_G	0.840	0.32
167_S	177_I	0.838	0.32
234_L	240_Y	0.832	0.32
134_T	184_K	0.830	0.32
23_K	204_G	0.824	0.31
89_V	134_T	0.823	0.31
145_M	154_L	0.819	0.31
156_P	244_G	0.818	0.31
156_P	252_G	0.818	0.31
113_N	175_K	0.817	0.31
41_E	246_S	0.810	0.30
156_P	213_G	0.809	0.30
212_V	255_E	0.804	0.30
50_R	93_I	0.800	0.30
164_S	238_V	0.799	0.30
83_T	88_V	0.796	0.30
26_R	251_F	0.795	0.30
130_V	254_V	0.794	0.30
41_E	139_Q	0.792	0.29
29_F	43_K	0.790	0.29
114_V	118_E	0.788	0.29
130_V	239_N	0.786	0.29
76_T	125_A	0.784	0.29
35_T	216_V	0.777	0.29
166_A	254_V	0.775	0.29
41_E	168_H	0.770	0.28
30_L	37_S	0.770	0.28
10_K	16_I	0.765	0.28
156_P	242_G	0.755	0.28
29_F	171_R	0.753	0.27
203_Y	245_K	0.753	0.27
193_N	216_V	0.747	0.27
33_S	118_E	0.735	0.27
41_E	247_R	0.733	0.26
43_K	171_R	0.732	0.26
51_I	144_R	0.731	0.26
75_N	238_V	0.729	0.26
16_I	106_S	0.722	0.26
161_L	165_I	0.721	0.26
32_F	91_E	0.720	0.26
37_S	181_T	0.715	0.26
169_W	173_M	0.710	0.25
111_L	182_G	0.709	0.25
165_I	169_W	0.705	0.25
26_R	137_L	0.705	0.25
8_R	205_N	0.703	0.25
131_H	137_L	0.702	0.25
145_M	158_V	0.700	0.25
190_R	228_R	0.700	0.25
194_Y	254_V	0.700	0.25
113_N	220_S	0.694	0.25
116_V	191_E	0.690	0.24
40_I	253_E	0.689	0.24
229_E	248_N	0.689	0.24
108_H	199_M	0.688	0.24
131_H	171_R	0.688	0.24
132_F	211_F	0.687	0.24
200_T	209_K	0.687	0.24
71_L	77_Y	0.685	0.24
226_K	230_N	0.684	0.24
102_I	116_V	0.683	0.24
196_I	246_S	0.679	0.24
168_H	246_S	0.676	0.24
123_E	133_K	0.674	0.24
117_K	212_V	0.673	0.24
133_K	212_V	0.670	0.23
9_L	203_Y	0.669	0.23
189_F	219_L	0.667	0.23
21_S	30_L	0.666	0.23
128_Y	256_V	0.666	0.23
85_L	92_V	0.666	0.23
88_V	240_Y	0.662	0.23
57_Q	112_V	0.661	0.23
128_Y	231_I	0.661	0.23
50_R	84_L	0.659	0.23
122_A	256_V	0.656	0.23
32_F	45_P	0.655	0.23
184_K	187_Y	0.653	0.23
9_L	15_V	0.651	0.23
74_E	77_Y	0.649	0.22
208_N	249_I	0.648	0.22
81_V	229_E	0.647	0.22
195_R	221_A	0.645	0.22
81_V	92_V	0.645	0.22
108_H	111_L	0.641	0.22
39_I	197_R	0.640	0.22
79_F	202_Y	0.640	0.22
27_T	181_T	0.638	0.22
33_S	222_R	0.638	0.22
130_V	231_I	0.636	0.22
23_K	251_F	0.634	0.22
138_L	226_K	0.634	0.22
26_R	30_L	0.631	0.22
20_F	140_P	0.630	0.22
100_R	197_R	0.629	0.22
166_A	234_L	0.629	0.22
139_Q	251_F	0.626	0.21
88_V	225_S	0.625	0.21
27_T	245_K	0.625	0.21
194_Y	249_I	0.625	0.21
86_E	112_V	0.624	0.21
21_S	24_L	0.624	0.21
20_F	104_N	0.624	0.21
100_R	104_N	0.623	0.21
75_N	85_L	0.621	0.21
168_H	241_F	0.618	0.21
185_T	242_G	0.618	0.21
13_N	125_A	0.612	0.21
114_V	174_D	0.609	0.21
41_E	137_L	0.608	0.21
23_K	245_K	0.607	0.21
33_S	127_F	0.602	0.20
71_L	190_R	0.599	0.20
167_S	222_R	0.599	0.20
34_P	204_G	0.595	0.20
23_K	201_A	0.595	0.20
71_L	211_F	0.591	0.20
154_L	196_I	0.589	0.20
107_F	111_L	0.586	0.20
20_F	193_N	0.586	0.20
243_V	249_I	0.585	0.20
119_N	233_R	0.581	0.20
183_S	189_F	0.580	0.20
98_V	243_V	0.580	0.20
174_D	217_F	0.579	0.20
6_T	16_I	0.579	0.20
209_K	241_F	0.577	0.19
22_S	192_V	0.577	0.19
30_L	249_I	0.574	0.19
13_N	19_P	0.573	0.19
97_S	111_L	0.572	0.19
77_Y	166_A	0.571	0.19
44_E	101_E	0.571	0.19
111_L	190_R	0.570	0.19
194_Y	243_V	0.568	0.19
9_L	110_E	0.566	0.19
81_V	115_S	0.564	0.19
211_F	234_L	0.563	0.19
57_Q	126_R	0.563	0.19
14_D	72_K	0.561	0.19
154_L	211_F	0.559	0.19
5_L	13_N	0.558	0.19
138_L	198_P	0.556	0.19
160_L	245_K	0.555	0.19
204_G	216_V	0.555	0.19
22_S	228_R	0.553	0.18
6_T	80_I	0.551	0.18
27_T	49_L	0.551	0.18
165_I	254_V	0.550	0.18
30_L	161_L	0.547	0.18
234_L	244_G	0.546	0.18
234_L	252_G	0.546	0.18
93_I	113_N	0.546	0.18
76_T	94_R	0.545	0.18
119_N	215_V	0.544	0.18
164_S	221_A	0.541	0.18
169_W	172_Y	0.541	0.18
90_K	198_P	0.540	0.18
22_S	89_V	0.537	0.18
141_P	229_E	0.537	0.18
83_T	92_V	0.534	0.18
79_F	102_I	0.530	0.18
8_R	15_V	0.529	0.18
93_I	175_K	0.529	0.18
112_V	219_L	0.529	0.18
84_L	138_L	0.527	0.18
213_G	242_G	0.526	0.17
11_P	127_F	0.525	0.17
155_F	193_N	0.524	0.17
139_Q	246_S	0.522	0.17
7_F	75_N	0.518	0.17
23_K	30_L	0.517	0.17
11_P	17_I	0.515	0.17
96_E	113_N	0.512	0.17
39_I	42_S	0.510	0.17
56_D	254_V	0.508	0.17
23_K	203_Y	0.508	0.17
74_E	117_K	0.506	0.17
200_T	249_I	0.504	0.17
108_H	134_T	0.504	0.17
47_K	219_L	0.501	0.17
26_R	168_H	0.500	0.17

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4illA	2	0.9617	100	0.379	Contact Map
4c98A	2	0.8161	100	0.449	Contact Map
4c97A	1	0.8352	100	0.459	Contact Map
3i4hX	1	0.8276	100	0.556	Contact Map
3ufcX	1	0.8812	100	0.561	Contact Map
4w8zA	1	0.4981	85.9	0.927	Contact Map
4l6uA	1	0.4904	74.8	0.935	Contact Map
4qtsA	1	0.613	29.9	0.951	Contact Map
4qtsC	1	0.4828	22.7	0.954	Contact Map
3x1lH	1	0.6973	13.8	0.958	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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