GREMLIN Database
CSA2B - CRISPR-associated aCascade subunit Cas7/Csa2 2
UniProt: Q97Y91 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 321 (277)
Sequences: 100 (85)
Seq/√Len: 5.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
73_G76_S7.5851.00
177_V237_K3.2040.77
73_G115_D3.1080.74
57_Y121_G2.8660.68
73_G118_A2.8120.66
84_F118_A2.7870.66
196_A236_G2.7820.65
58_Q83_K2.7720.65
17_G115_D2.6370.61
17_G52_A2.6180.60
32_V77_S2.5930.60
74_S77_S2.5520.58
61_L116_V2.4510.55
83_K249_S2.4070.54
76_S115_D2.3660.52
66_K77_S2.3460.52
15_L243_P2.3020.50
271_K275_M2.2750.49
76_S118_A2.2540.49
124_M186_F2.1860.46
13_E83_K2.1200.44
274_I278_G2.0850.43
83_K121_G2.0200.41
13_E182_A2.0120.41
46_P240_R2.0050.41
211_L264_A1.9720.39
61_L70_L1.9430.39
16_N236_G1.9430.39
184_Y215_K1.9280.38
20_S142_M1.8730.36
178_E240_R1.8520.36
2_I269_Y1.8330.35
22_G133_R1.7700.33
180_S223_I1.7160.32
61_L117_I1.6990.31
55_H243_P1.6920.31
51_E83_K1.6680.30
131_V254_K1.6100.29
53_L142_M1.6050.29
46_P144_P1.6000.29
69_G106_R1.5800.28
54_A57_Y1.5690.28
139_L157_A1.5570.27
5_S147_R1.5490.27
137_I188_F1.5160.26
79_Y260_M1.5140.26
277_L291_A1.5090.26
28_R263_P1.5010.26
27_H182_A1.5010.26
47_V251_V1.4990.26
144_P240_R1.4980.26
5_S35_K1.4890.26
160_H258_P1.4880.26
65_A71_P1.4870.26
19_E26_K1.4850.26
51_E121_G1.4780.25
112_M198_P1.4750.25
17_G198_P1.4730.25
141_Y263_P1.4690.25
14_S237_K1.4680.25
115_D236_G1.4680.25
68_E224_K1.4300.24
19_E54_A1.4290.24
219_V251_V1.4260.24
14_S17_G1.4230.24
7_R240_R1.4190.24
72_V182_A1.4130.24
76_S137_I1.3980.23
92_I291_A1.3950.23
17_G141_Y1.3760.23
178_E237_K1.3720.23
238_R242_L1.3580.23
64_I221_S1.3530.22
161_V263_P1.3340.22
197_V247_L1.3310.22
42_V244_S1.3310.22
215_K219_V1.3260.22
211_L216_A1.3250.22
62_V132_R1.3230.22
206_K250_L1.3210.22
30_A115_D1.3170.22
51_E241_F1.3140.22
83_K187_S1.3140.22
19_E83_K1.3120.22
30_A73_G1.3110.22
24_L244_S1.3080.21
123_F162_R1.2990.21
141_Y178_E1.2910.21
48_I142_M1.2710.21
20_S161_V1.2570.20
214_Q217_K1.2550.20
123_F237_K1.2520.20
61_L182_A1.2420.20
15_L55_H1.2400.20
13_E141_Y1.2370.20
121_G137_I1.2310.20
72_V168_V1.2220.20
274_I292_Y1.2180.20
18_V182_A1.2170.19
13_E19_E1.2110.19
19_E58_Q1.2110.19
6_V248_M1.2080.19
64_I69_G1.1940.19
36_T274_I1.1900.19
16_N48_I1.1880.19
28_R49_S1.1770.19
17_G236_G1.1620.18
46_P108_E1.1610.18
108_E144_P1.1610.18
158_Q162_R1.1500.18
159_F181_S1.1380.18
161_V173_A1.1360.18
217_K220_K1.1360.18
19_E177_V1.1240.18
69_G214_Q1.1140.17
222_A225_A1.0980.17
172_Q176_N1.0960.17
27_H46_P1.0930.17
133_R243_P1.0920.17
22_G243_P1.0920.17
93_E113_L1.0850.17
19_E52_A1.0830.17
29_T149_D1.0790.17
31_P141_Y1.0650.17
126_A191_D1.0650.17
157_A295_N1.0630.17
77_S120_V1.0560.16
220_K224_K1.0540.16
84_F225_A1.0530.16
179_V241_F1.0530.16
54_A83_K1.0510.16
191_D253_T1.0510.16
69_G288_L1.0470.16
141_Y177_V1.0370.16
5_S264_A1.0270.16
79_Y226_L1.0230.16
158_Q163_F1.0220.16
111_V125_Y1.0200.16
13_E54_A1.0130.16
18_V141_Y1.0120.16
48_I69_G1.0090.16
146_L259_F1.0040.15
196_A223_I0.9830.15
13_E52_A0.9770.15
102_N113_L0.9770.15
83_K141_Y0.9720.15
27_H51_E0.9700.15
147_R291_A0.9690.15
215_K244_S0.9680.15
132_R191_D0.9660.15
134_T177_V0.9550.15
2_I192_E0.9540.15
160_H184_Y0.9520.15
48_I140_G0.9500.15
254_K281_K0.9460.15
16_N49_S0.9440.14
27_H144_P0.9430.14
47_V172_Q0.9390.14
223_I248_M0.9380.14
57_Y222_A0.9340.14
126_A280_A0.9270.14
259_F276_R0.9260.14
49_S65_A0.9220.14
92_I102_N0.9210.14
54_A185_T0.9160.14
17_G157_A0.9150.14
93_E112_M0.9120.14
68_E71_P0.9060.14
34_L244_S0.9060.14
24_L178_E0.9020.14
134_T172_Q0.8980.14
55_H223_I0.8940.14
62_V79_Y0.8920.14
57_Y229_L0.8920.14
73_G84_F0.8910.14
48_I96_K0.8890.14
132_R237_K0.8870.14
144_P178_E0.8860.14
27_H52_A0.8850.14
50_G122_G0.8840.14
119_D218_R0.8840.14
50_G218_R0.8840.14
159_F208_E0.8840.14
122_G218_R0.8840.14
119_D122_G0.8840.14
50_G119_D0.8840.14
112_M255_T0.8830.14
28_R141_Y0.8780.13
27_H178_E0.8780.13
136_R214_Q0.8720.13
13_E187_S0.8710.13
81_F141_Y0.8710.13
54_A141_Y0.8710.13
146_L260_M0.8650.13
54_A72_V0.8620.13
61_L269_Y0.8620.13
66_K277_L0.8610.13
10_V264_A0.8600.13
54_A121_G0.8600.13
291_A296_N0.8570.13
133_R160_H0.8550.13
22_G160_H0.8550.13
3_S28_R0.8540.13
57_Y225_A0.8460.13
102_N277_L0.8450.13
33_V87_D0.8440.13
6_V186_F0.8400.13
28_R65_A0.8380.13
25_T175_F0.8360.13
16_N115_D0.8360.13
69_G213_R0.8340.13
65_A117_I0.8310.13
51_E58_Q0.8300.13
25_T190_L0.8290.13
141_Y172_Q0.8270.13
41_L254_K0.8260.13
19_E141_Y0.8260.13
49_S236_G0.8250.13
141_Y240_R0.8250.13
54_A243_P0.8230.13
81_F239_S0.8220.13
3_S261_P0.8220.13
92_I271_K0.8200.13
52_A276_R0.8140.12
124_M127_G0.8120.12
193_D213_R0.8100.12
91_K295_N0.8090.12
30_A47_V0.8070.12
19_E57_Y0.8030.12
81_F161_V0.8000.12
56_A273_T0.7990.12
44_Y56_A0.7930.12
115_D263_P0.7920.12
190_L222_A0.7920.12
4_G268_D0.7900.12
11_N55_H0.7870.12
76_S199_S0.7820.12
14_S177_V0.7820.12
178_E244_S0.7820.12
239_S273_T0.7800.12
121_G238_R0.7770.12
10_V211_L0.7760.12
8_F60_S0.7750.12
181_S229_L0.7670.12
32_V214_Q0.7600.12
34_L42_V0.7600.12
29_T230_L0.7590.12
156_E174_I0.7590.12
30_A76_S0.7570.12
27_H240_R0.7560.12
264_A292_Y0.7420.11
131_V211_L0.7390.11
113_L211_L0.7370.11
21_I26_K0.7320.11
74_S207_G0.7320.11
46_P178_E0.7310.11
143_I292_Y0.7290.11
140_G266_D0.7290.11
173_A263_P0.7280.11
85_S180_S0.7280.11
72_V142_M0.7280.11
131_V181_S0.7260.11
3_S64_I0.7230.11
57_Y238_R0.7190.11
26_K48_I0.7180.11
137_I228_S0.7180.11
112_M180_S0.7160.11
120_V221_S0.7150.11
112_M191_D0.7030.11
131_V197_V0.6990.11
7_R48_I0.6970.11
179_V238_R0.6960.11
93_E127_G0.6940.11
135_S243_P0.6930.11
90_L210_E0.6910.11
134_T178_E0.6910.11
14_S120_V0.6890.11
51_E158_Q0.6880.11
161_V186_F0.6880.11
2_I43_R0.6880.11
54_A177_V0.6830.11
268_D292_Y0.6820.11
116_V210_E0.6800.11
30_A231_S0.6790.11
23_N184_Y0.6780.11
115_D118_A0.6760.11
2_I283_V0.6750.11
48_I103_D0.6740.11
36_T113_L0.6740.11
175_F179_V0.6730.11
6_V250_L0.6700.11
58_Q125_Y0.6690.11
177_V238_R0.6670.11
31_P44_Y0.6620.10
82_I169_S0.6580.10
190_L252_V0.6530.10
14_S52_A0.6510.10
134_T141_Y0.6480.10
124_M229_L0.6470.10
45_V221_S0.6460.10
110_E289_A0.6440.10
13_E57_Y0.6410.10
210_E223_I0.6410.10
115_D198_P0.6410.10
141_Y199_S0.6400.10
106_R143_I0.6400.10
17_G47_V0.6390.10
262_E276_R0.6360.10
11_N108_E0.6330.10
79_Y146_L0.6320.10
87_D275_M0.6290.10
249_S269_Y0.6210.10
140_G156_E0.6190.10
72_V230_L0.6180.10
207_G279_K0.6170.10
116_V129_A0.6170.10
16_N28_R0.6170.10
57_Y141_Y0.6160.10
110_E113_L0.6140.10
131_V150_E0.6140.10
126_A294_I0.6120.10
2_I165_N0.6100.10
13_E27_H0.6080.10
35_K170_G0.6080.10
7_R237_K0.6080.10
57_Y81_F0.6070.10
14_S176_N0.6050.10
182_A262_E0.6030.10
211_L283_V0.6030.10
51_E141_Y0.6000.10
44_Y273_T0.6000.10
108_E250_L0.5990.10
48_I177_V0.5980.10
141_Y237_K0.5940.10
74_S101_Y0.5920.10
13_E18_V0.5900.10
128_G282_G0.5880.10
237_K240_R0.5880.10
2_I65_A0.5870.10
60_S229_L0.5860.10
174_I275_M0.5860.10
236_G244_S0.5850.10
225_A281_K0.5840.10
66_K74_S0.5830.10
102_N271_K0.5810.10
112_M222_A0.5810.10
53_L193_D0.5780.09
175_F285_N0.5770.09
143_I242_L0.5710.09
117_I226_L0.5640.09
137_I140_G0.5630.09
188_F226_L0.5630.09
83_K239_S0.5610.09
77_S183_L0.5600.09
143_I181_S0.5560.09
102_N268_D0.5550.09
133_R218_R0.5550.09
22_G122_G0.5550.09
50_G133_R0.5550.09
119_D133_R0.5550.09
22_G50_G0.5550.09
122_G133_R0.5550.09
22_G218_R0.5550.09
22_G119_D0.5550.09
69_G275_M0.5520.09
185_T266_D0.5510.09
57_Y259_F0.5510.09
6_V272_T0.5500.09
18_V52_A0.5500.09
76_S104_A0.5490.09
62_V130_P0.5480.09
57_Y182_A0.5460.09
2_I112_M0.5460.09
194_L197_V0.5430.09
168_V220_K0.5410.09
81_F108_E0.5410.09
17_G263_P0.5410.09
268_D282_G0.5410.09
106_R109_V0.5400.09
217_K291_A0.5390.09
108_E240_R0.5360.09
76_S129_A0.5360.09
26_K264_A0.5340.09
48_I63_D0.5340.09
55_H83_K0.5300.09
28_R117_I0.5260.09
27_H237_K0.5240.09
106_R249_S0.5230.09
91_K102_N0.5230.09
57_Y83_K0.5220.09
16_N47_V0.5220.09
216_A256_D0.5210.09
53_L127_G0.5210.09
140_G222_A0.5190.09
134_T144_P0.5180.09
3_S221_S0.5180.09
48_I156_E0.5160.09
125_Y131_V0.5150.09
88_E226_L0.5150.09
183_L195_I0.5130.09
124_M250_L0.5110.09
46_P79_Y0.5110.09
16_N196_A0.5100.09
7_R26_K0.5090.09
275_M278_G0.5090.09
14_S111_V0.5090.09
69_G212_E0.5070.09
83_K125_Y0.5050.09
28_R172_Q0.5050.09
148_G295_N0.5040.09
157_A289_A0.5030.09
85_S223_I0.5030.09
49_S61_L0.5010.09
45_V58_Q0.5010.09
6_V53_L0.5000.09
69_G221_S0.5000.09
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3ps0A 1 0.9283 100 0.301 Contact Map
4u7uE 3 0.8411 88.8 0.954 Contact Map
4txdA 1 0.8318 38.5 0.969 Contact Map
3k7nA 1 0.2492 24.9 0.972 Contact Map
3k7lA 1 0.2679 16.3 0.974 Contact Map
4qtsC 1 0.5514 11.7 0.976 Contact Map
2eroA 2 0.271 10.2 0.976 Contact Map
3wwvA 2 0.1433 9.3 0.977 Contact Map
3k59A 1 0.7913 9.3 0.977 Contact Map
3o2pE 1 0.2679 8.5 0.977 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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