GREMLIN Database
CAS1B - CRISPR-associated endonuclease Cas1 2
UniProt: Q97Y84 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 307 (294)
Sequences: 2018 (1560)
Seq/√Len: 91.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
114_I145_A5.4051.00
181_S227_I5.3111.00
181_S256_L4.9071.00
95_E98_V3.0321.00
249_E252_S3.0211.00
238_G252_S3.0191.00
17_Y26_K2.9731.00
238_G255_D2.9551.00
59_L76_G2.8761.00
55_L65_V2.8431.00
36_P63_I2.8081.00
186_Y220_S2.5741.00
238_G249_E2.5351.00
167_D171_T2.5121.00
92_A95_E2.4401.00
57_L61_H2.3941.00
184_I260_I2.3641.00
170_G179_N2.3031.00
189_I220_S2.2981.00
248_I252_S2.2491.00
136_E139_D2.2301.00
188_T260_I2.2101.00
91_K208_D2.2081.00
181_S231_L2.1981.00
269_K276_P2.1571.00
16_V54_A2.0801.00
36_P57_L2.0021.00
185_L227_I1.9721.00
23_L54_A1.9561.00
177_A235_L1.9461.00
277_K285_A1.9461.00
140_K143_P1.9161.00
18_V57_L1.8821.00
96_A294_I1.8801.00
134_Y137_L1.8791.00
212_N217_Y1.8711.00
43_L68_L1.8481.00
123_Y229_F1.8461.00
15_Y49_S1.8051.00
259_L262_K1.7911.00
91_K97_I1.7901.00
258_K262_K1.7661.00
87_V202_L1.7551.00
27_K32_V1.7481.00
105_Y109_I1.7431.00
263_G266_E1.7091.00
193_L216_V1.7051.00
174_F231_L1.7021.00
90_K199_D1.6751.00
22_M33_E1.6741.00
44_I48_C1.6741.00
42_I58_A1.6411.00
117_Q146_A1.6391.00
103_N107_E1.6331.00
7_L42_I1.6141.00
133_D137_L1.5951.00
103_N138_L1.5931.00
96_A101_K1.5921.00
135_K139_D1.5721.00
177_A231_L1.5551.00
68_L195_I1.5541.00
149_Y228_D1.5531.00
174_F235_L1.5391.00
8_V45_T1.5261.00
15_Y51_S1.5221.00
56_S75_W1.4991.00
250_E254_G1.4881.00
219_F287_A1.4611.00
23_L36_P1.4571.00
92_A96_A1.4330.99
173_Q241_L1.4270.99
234_A259_L1.4230.99
108_E299_E1.4220.99
222_M286_H1.4100.99
54_A58_A1.3890.99
231_L256_L1.3870.99
42_I65_V1.3850.99
238_G248_I1.3740.99
190_F216_V1.3540.99
23_L42_I1.3520.99
23_L58_A1.3470.99
11_E27_K1.3470.99
161_I235_L1.3410.99
117_Q225_P1.3410.99
19_K33_E1.3270.99
269_K274_H1.3150.99
14_A25_I1.3000.99
284_R288_V1.2980.99
262_K265_E1.2830.99
69_N73_T1.2830.99
189_I283_I1.2710.99
135_K207_K1.2700.99
268_V282_A1.2660.99
275_N281_Q1.2620.99
10_S257_A1.2620.99
12_Y46_V1.2510.98
14_A27_K1.2490.98
24_V33_E1.2480.98
195_I284_R1.2470.98
13_G48_C1.2380.98
65_V78_L1.2260.98
139_D205_I1.2220.98
113_K228_D1.2180.98
193_L287_A1.2170.98
144_A147_R1.2130.98
267_N276_P1.2070.98
260_I264_M1.1970.98
186_Y217_Y1.1970.98
104_R299_E1.1890.98
222_M300_Y1.1880.98
147_R151_Q1.1780.98
120_H225_P1.1760.98
261_R280_I1.1720.98
66_M77_I1.1690.98
223_F282_A1.1680.98
58_A65_V1.1680.98
7_L39_V1.1610.97
36_P58_A1.1530.97
103_N134_Y1.1460.97
103_N132_N1.1440.97
91_K94_Y1.1380.97
109_I112_S1.1320.97
154_S172_D1.1300.97
241_L246_G1.1280.97
220_S224_K1.1160.97
110_I205_I1.1080.97
101_K104_R1.1070.97
121_L152_N1.1070.97
184_I257_A1.0990.96
47_S53_S1.0950.96
180_Y256_L1.0940.96
180_Y257_A1.0910.96
234_A255_D1.0900.96
180_Y253_R1.0660.96
124_W156_L1.0600.96
26_K31_K1.0600.96
148_V151_Q1.0580.96
143_P221_E1.0560.96
178_L231_L1.0440.95
154_S162_G1.0400.95
6_T43_L1.0370.95
85_E210_P1.0370.95
18_V36_P1.0310.95
12_Y70_S1.0230.95
157_L174_F1.0200.95
193_L219_F1.0170.95
250_E253_R1.0160.95
279_L282_A1.0090.94
23_L63_I1.0090.94
227_I231_L1.0020.94
64_S77_I0.9950.94
188_T280_I0.9930.94
90_K94_Y0.9920.94
102_D207_K0.9880.94
66_M187_N0.9860.94
50_I54_A0.9840.94
109_I222_M0.9820.94
21_N61_H0.9690.93
118_S122_K0.9680.93
153_I174_F0.9670.93
113_K197_G0.9660.93
144_A148_V0.9650.93
141_D144_A0.9610.93
113_K146_A0.9540.92
105_Y108_E0.9530.92
146_A228_D0.9480.92
42_I63_I0.9480.92
91_K98_V0.9460.92
177_A248_I0.9370.92
182_Y228_D0.9370.92
270_E285_A0.9330.92
41_E64_S0.9150.91
119_V122_K0.9120.91
113_K224_K0.9080.91
105_Y290_L0.9060.90
55_L78_L0.9040.90
213_E217_Y0.9040.90
124_W232_V0.9030.90
106_G205_I0.9030.90
274_H297_G0.9020.90
185_L220_S0.8950.90
36_P61_H0.8950.90
226_Y279_L0.8920.90
186_Y213_E0.8890.90
12_Y47_S0.8840.89
6_T41_E0.8830.89
121_L149_Y0.8730.89
181_S230_L0.8690.89
17_Y51_S0.8650.88
132_N137_L0.8620.88
188_T264_M0.8620.88
238_G251_N0.8570.88
192_Y196_A0.8560.88
21_N57_L0.8480.87
214_S217_Y0.8450.87
110_I138_L0.8400.87
85_E199_D0.8310.86
120_H232_V0.8300.86
186_Y190_F0.8220.86
171_T245_G0.8210.86
178_L182_Y0.8170.85
233_R237_S0.8130.85
74_P77_I0.8110.85
97_I102_D0.7990.84
267_N278_T0.7940.84
108_E112_S0.7930.83
266_E269_K0.7900.83
275_N288_V0.7890.83
257_A261_R0.7880.83
286_H300_Y0.7880.83
120_H124_W0.7870.83
108_E111_S0.7840.83
259_L266_E0.7840.83
281_Q284_R0.7830.83
104_R107_E0.7830.83
5_R38_E0.7830.83
183_A187_N0.7820.83
37_S61_H0.7800.82
190_F213_E0.7770.82
255_D258_K0.7770.82
107_E132_N0.7720.82
255_D259_L0.7710.82
157_L235_L0.7700.82
132_N141_D0.7670.81
27_K30_K0.7670.81
268_V277_K0.7580.81
49_S53_S0.7570.81
284_R289_K0.7570.81
105_Y293_S0.7570.81
270_E289_K0.7520.80
291_A295_R0.7510.80
18_V54_A0.7490.80
50_I55_L0.7480.80
23_L39_V0.7470.80
173_Q246_G0.7440.79
12_Y48_C0.7440.79
168_V179_N0.7440.79
287_A291_A0.7420.79
270_E277_K0.7410.79
9_I14_A0.7400.79
100_R103_N0.7390.79
148_V159_K0.7390.79
139_D207_K0.7370.79
28_G148_V0.7330.78
56_S60_T0.7330.78
197_G291_A0.7320.78
177_A256_L0.7250.78
24_V31_K0.7240.78
176_M246_G0.7220.77
115_Y119_V0.7220.77
142_E206_H0.7200.77
104_R108_E0.7200.77
90_K210_P0.7190.77
137_L141_D0.7140.77
251_N255_D0.7130.76
41_E66_M0.7130.76
183_A223_F0.7120.76
298_K301_R0.7100.76
201_Y216_V0.7080.76
166_R172_D0.7070.76
34_I42_I0.7070.76
150_W182_Y0.7040.76
277_K282_A0.7020.75
221_E224_K0.7020.75
113_K182_Y0.6990.75
12_Y53_S0.6970.75
106_G135_K0.6850.74
62_G196_A0.6830.73
158_P236_R0.6830.73
289_K301_R0.6810.73
230_L260_I0.6800.73
59_L78_L0.6790.73
58_A63_I0.6730.72
85_E211_G0.6690.72
153_I161_I0.6670.72
203_G208_D0.6650.71
117_Q224_K0.6650.71
169_D205_I0.6640.71
120_H229_F0.6620.71
50_I65_V0.6600.71
55_L67_F0.6590.71
158_P161_I0.6580.71
92_A101_K0.6580.71
201_Y213_E0.6580.71
103_N207_K0.6540.70
254_G258_K0.6530.70
57_L60_T0.6530.70
91_K207_K0.6520.70
274_H299_E0.6520.70
53_S56_S0.6490.70
171_T244_K0.6480.69
240_R249_E0.6480.69
97_I202_L0.6470.69
54_A57_L0.6450.69
159_K162_G0.6430.69
109_I204_F0.6430.69
89_T295_R0.6420.69
122_K126_R0.6390.68
125_A129_G0.6360.68
141_D148_V0.6360.68
271_E285_A0.6350.68
107_E111_S0.6340.68
85_E209_R0.6340.68
178_L224_K0.6310.67
13_G260_I0.6310.67
240_R252_S0.6290.67
91_K102_D0.6260.67
8_V261_R0.6260.67
55_L59_L0.6260.67
63_I148_V0.6250.67
153_I232_V0.6250.67
45_T72_E0.6230.66
290_L294_I0.6230.66
252_S255_D0.6220.66
69_N75_W0.6200.66
234_A256_L0.6200.66
230_L234_A0.6170.66
94_Y202_L0.6160.66
246_G253_R0.6130.65
136_E140_K0.6120.65
106_G109_I0.6110.65
100_R135_K0.6080.65
181_S185_L0.6080.65
38_E151_Q0.6060.64
281_Q285_A0.6040.64
87_V91_K0.6030.64
251_N254_G0.6020.64
51_S54_A0.6020.64
217_Y220_S0.6010.64
275_N284_R0.6010.64
102_D135_K0.6000.63
112_S222_M0.6000.63
85_E89_T0.5990.63
293_S296_E0.5990.63
123_Y127_V0.5980.63
60_T172_D0.5950.63
202_L208_D0.5940.63
23_L92_A0.5930.63
53_S70_S0.5930.63
278_T281_Q0.5930.63
113_K221_E0.5910.62
197_G224_K0.5890.62
180_Y183_A0.5890.62
101_K105_Y0.5860.62
114_I141_D0.5830.61
264_M283_I0.5830.61
206_H221_E0.5820.61
10_S45_T0.5810.61
265_E280_I0.5800.61
284_R292_S0.5800.61
75_W78_L0.5800.61
117_Q149_Y0.5780.61
169_D217_Y0.5760.60
185_L260_I0.5730.60
116_N229_F0.5720.60
283_I301_R0.5720.60
7_L23_L0.5720.60
146_A182_Y0.5720.60
123_Y126_R0.5710.60
95_E204_F0.5700.60
106_G215_L0.5690.59
97_I207_K0.5690.59
168_V225_P0.5690.59
85_E90_K0.5670.59
263_G279_L0.5660.59
204_F290_L0.5640.59
199_D210_P0.5630.59
144_A296_E0.5620.58
146_A224_K0.5610.58
173_Q176_M0.5610.58
192_Y287_A0.5610.58
157_L161_I0.5590.58
15_Y58_A0.5570.58
252_S256_L0.5570.58
123_Y232_V0.5530.57
66_M74_P0.5530.57
19_K24_V0.5520.57
35_S38_E0.5510.57
154_S165_G0.5480.57
76_G153_I0.5480.57
17_Y24_V0.5460.56
154_S158_P0.5450.56
154_S164_D0.5440.56
152_N155_Q0.5430.56
203_G216_V0.5420.56
67_F78_L0.5420.56
256_L259_L0.5410.56
74_P194_V0.5400.56
126_R152_N0.5400.56
87_V94_Y0.5390.55
106_G139_D0.5390.55
87_V208_D0.5390.55
198_L215_L0.5370.55
190_F200_P0.5370.55
230_L259_L0.5370.55
27_K31_K0.5360.55
21_N37_S0.5360.55
170_G212_N0.5350.55
203_G210_P0.5350.55
233_R263_G0.5330.55
196_A288_V0.5330.55
143_P170_G0.5310.54
68_L78_L0.5310.54
201_Y208_D0.5300.54
9_I122_K0.5300.54
94_Y98_V0.5290.54
248_I260_I0.5260.54
140_K144_A0.5260.54
189_I219_F0.5250.53
143_P224_K0.5250.53
109_I290_L0.5240.53
36_P54_A0.5240.53
44_I67_F0.5240.53
57_L63_I0.5240.53
144_A282_A0.5230.53
192_Y195_I0.5210.53
92_A171_T0.5190.53
9_I54_A0.5170.52
153_I158_P0.5160.52
271_E294_I0.5160.52
280_I283_I0.5150.52
132_N138_L0.5130.52
89_T294_I0.5100.51
242_K247_L0.5100.51
240_R247_L0.5090.51
164_D171_T0.5090.51
109_I205_I0.5090.51
113_K117_Q0.5090.51
139_D179_N0.5080.51
89_T212_N0.5070.51
47_S51_S0.5060.51
22_M37_S0.5060.51
277_K293_S0.5060.51
239_F246_G0.5050.51
107_E269_K0.5040.51
199_D217_Y0.5030.50
97_I204_F0.5020.50
189_I192_Y0.5020.50
90_K202_L0.5010.50
13_G47_S0.5010.50
133_D136_E0.5000.50
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4wj0A 2 0.9805 100 0.143 Contact Map
4n06A 2 0.9837 100 0.159 Contact Map
4xtkA 2 0.987 100 0.162 Contact Map
2yzsA 4 0.9739 100 0.166 Contact Map
4w8kA 2 0.8534 100 0.339 Contact Map
3godA 4 0.8795 100 0.398 Contact Map
3nkdA 2 0.8371 100 0.424 Contact Map
3nkeA 3 0.5896 100 0.575 Contact Map
1fw8A 1 0.2248 78.4 0.95 Contact Map
2iojA 1 0.2899 35.5 0.962 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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