Q97Y67 - SULSO - GREMLIN Contacts

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Q97Y67 - Transposase ISC1476

UniProt:	Q97Y67	Sequences: MKRSNTVRLVVSKEQHEVLKRLGIATAKCWNEVNWLRMKQYKEGKRVDFGITEKAVYEKYKSVLRVNAQQVARKNAEDWRSFFSLTREKKEGKLPSWFKPRPPGYWKDGRLIVIIRNDRYTVDERNRVVYLKDFNLSLKFKGRLKWHGKQGRLEIIYDEARKIWYAHIPVEVETEKVKEGIKASIDLGIVNLATVYVEDGSWYLFKGGSALSHYENYSKRITIVQKTLHRHRQRRSRRLKLLHEKRSRFLKHALNSMVRRIMEILREKNVGEVVVGYPKEIAKHHGNKLTVNFWNYSYIIKRLEDIGEELGIKVITVDEAYTSKTCSLCGEAHEDGRVKRGLFVCPRTRKAINADLNGAINILHIPESRGSEGNFHRGIGVIGLKTQPVTLSDLSKIKNHYNYSISDYTILHYIQP	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	416 (348)
Sequences:	4485 (2888)
Seq/√Len:	154.8

ECOLI - INSQ
HALSA - Q9HSW9 Q9HHJ9 Q9HM87 Q9HSW7 Q9HHG8 Q9HSZ6 Q9HSD4 Q9HHU6 Q9HSY4 Q9HHX8
SULSO - Q97Y67 Q97WZ3 Q97YU1 Q97XP1 Q7LXD9 Q97X39 Q97XQ0 Q97YI2 Q97Y44 Q97XQ8 Q97U51 Q97Z61 Q7LXH2 Q97V47 Q97YI5 Q97YW9 Q97UQ8 Q97Y48 Q97V25 Q97XB6 Q97YW4 Q97U68 Q97V53 Q97WY6 Q97XA4 Q7LXH6 Q97YF7 Y2105 Q97U09 Q7LX59 Q7LXH9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
252_H	256_S	5.540	1.00
327_S	357_N	4.448	1.00
307_G	314_V	3.855	1.00
274_V	280_E	3.658	1.00
300_I	316_T	3.059	1.00
192_L	299_I	3.053	1.00
259_R	263_E	2.852	1.00
269_N	366_P	2.816	1.00
298_Y	302_R	2.794	1.00
3_R	309_E	2.683	1.00
298_Y	301_K	2.576	1.00
321_Y	324_K	2.533	1.00
299_I	303_L	2.503	1.00
153_L	166_A	2.499	1.00
214_Y	289_L	2.490	1.00
304_E	316_T	2.488	1.00
325_T	332_A	2.458	1.00
262_M	307_G	2.447	1.00
317_V	362_I	2.398	1.00
324_K	336_G	2.343	1.00
337_R	340_R	2.291	1.00
151_G	171_E	2.288	1.00
365_I	368_S	2.242	1.00
282_A	292_N	2.198	1.00
220_R	235_R	2.137	1.00
213_H	249_F	2.124	1.00
255_N	305_D	2.118	1.00
322_T	362_I	2.111	1.00
255_N	302_R	2.102	1.00
35_W	39_K	2.101	1.00
344_V	351_A	2.082	1.00
329_C	348_T	2.052	1.00
38_M	102_P	2.043	1.00
235_R	239_L	1.986	1.00
20_K	157_Y	1.984	1.00
326_C	329_C	1.978	1.00
258_V	302_R	1.943	1.00
33_V	72_A	1.939	1.00
21_R	62_S	1.937	1.00
20_K	164_W	1.925	1.00
53_E	56_V	1.887	1.00
266_R	312_I	1.885	1.00
12_S	15_Q	1.881	1.00
251_K	298_Y	1.880	1.00
329_C	345_C	1.855	1.00
230_R	233_Q	1.848	1.00
192_L	253_A	1.824	1.00
284_H	288_K	1.813	1.00
345_C	348_T	1.811	1.00
304_E	308_E	1.801	1.00
341_G	354_A	1.793	1.00
270_V	303_L	1.789	1.00
34_N	75_N	1.762	1.00
285_H	288_K	1.759	1.00
255_N	306_I	1.748	1.00
8_R	163_I	1.748	1.00
324_K	334_E	1.743	1.00
343_F	352_I	1.741	1.00
254_L	302_R	1.740	1.00
326_C	348_T	1.726	1.00
328_L	350_K	1.694	1.00
185_I	193_A	1.687	1.00
326_C	345_C	1.639	1.00
255_N	258_V	1.632	1.00
337_R	354_A	1.623	1.00
233_Q	236_S	1.610	1.00
19_L	164_W	1.609	1.00
128_V	166_A	1.584	1.00
333_H	336_G	1.553	1.00
254_L	298_Y	1.542	1.00
195_V	199_D	1.534	1.00
17_E	21_R	1.502	1.00
76_A	80_R	1.494	1.00
191_N	194_T	1.494	1.00
194_T	356_L	1.493	1.00
4_S	167_H	1.481	1.00
227_T	235_R	1.469	1.00
220_R	223_I	1.467	1.00
343_F	350_K	1.464	1.00
272_E	316_T	1.458	1.00
16_H	20_K	1.456	1.00
342_L	351_A	1.442	1.00
182_K	363_L	1.437	1.00
183_A	195_V	1.436	1.00
74_K	114_I	1.412	1.00
163_I	244_E	1.400	1.00
33_V	75_N	1.385	1.00
333_H	343_F	1.368	1.00
66_V	116_R	1.364	1.00
258_V	306_I	1.361	1.00
360_I	364_H	1.358	1.00
158_D	244_E	1.358	1.00
258_V	305_D	1.334	1.00
223_I	227_T	1.330	1.00
331_E	346_P	1.327	1.00
52_T	56_V	1.324	1.00
304_E	314_V	1.323	1.00
220_R	242_L	1.322	1.00
23_G	164_W	1.311	1.00
270_V	314_V	1.301	1.00
258_V	299_I	1.296	1.00
66_V	118_D	1.293	1.00
77_E	81_S	1.290	1.00
224_V	236_S	1.284	1.00
79_W	102_P	1.283	1.00
244_E	248_R	1.268	1.00
213_H	246_R	1.261	1.00
11_V	15_Q	1.259	1.00
291_V	296_Y	1.259	1.00
336_G	340_R	1.248	1.00
217_Y	239_L	1.246	1.00
51_I	56_V	1.241	1.00
327_S	360_I	1.222	1.00
270_V	300_I	1.220	1.00
281_I	284_H	1.219	1.00
26_T	71_V	1.219	1.00
13_K	16_H	1.217	1.00
262_M	310_L	1.212	1.00
161_R	244_E	1.206	1.00
80_R	84_S	1.200	1.00
224_V	239_L	1.199	1.00
327_S	352_I	1.195	1.00
274_V	319_E	1.193	1.00
356_L	360_I	1.192	1.00
338_V	341_G	1.186	1.00
333_H	346_P	1.185	1.00
275_V	297_S	1.161	1.00
260_R	264_I	1.156	1.00
141_K	144_L	1.156	1.00
187_L	296_Y	1.154	1.00
70_Q	73_R	1.152	1.00
196_Y	359_A	1.150	1.00
38_M	42_K	1.145	1.00
221_I	225_Q	1.136	1.00
280_E	284_H	1.136	1.00
183_A	265_L	1.134	1.00
115_I	130_Y	1.134	1.00
184_S	363_L	1.117	1.00
322_T	365_I	1.115	1.00
156_I	167_H	1.103	0.99
352_I	360_I	1.100	0.99
36_L	55_A	1.092	0.99
268_K	307_G	1.092	0.99
254_L	299_I	1.084	0.99
52_T	55_A	1.080	0.99
143_R	146_W	1.079	0.99
189_I	294_W	1.074	0.99
129_V	135_N	1.071	0.99
185_I	195_V	1.070	0.99
302_R	305_D	1.069	0.99
317_V	365_I	1.068	0.99
121_T	129_V	1.067	0.99
259_R	310_L	1.065	0.99
306_I	309_E	1.063	0.99
251_K	302_R	1.059	0.99
60_Y	68_A	1.053	0.99
194_T	200_G	1.052	0.99
30_W	71_V	1.051	0.99
317_V	366_P	1.036	0.99
187_L	300_I	1.036	0.99
81_S	87_R	1.035	0.99
39_K	43_E	1.034	0.99
82_F	102_P	1.033	0.99
158_D	161_R	1.030	0.99
217_Y	243_H	1.030	0.99
216_N	220_R	1.030	0.99
262_M	312_I	1.023	0.99
200_G	353_N	1.022	0.99
325_T	365_I	1.018	0.99
179_E	266_R	1.018	0.99
182_K	367_E	1.017	0.99
258_V	303_L	1.015	0.99
81_S	86_T	1.014	0.99
324_K	332_A	1.012	0.99
70_Q	116_R	1.008	0.99
113_V	117_N	1.005	0.99
6_T	158_D	1.005	0.99
272_E	319_E	0.997	0.99
263_E	310_L	0.994	0.99
64_L	67_N	0.992	0.99
218_S	222_T	0.984	0.99
220_R	239_L	0.983	0.99
352_I	356_L	0.977	0.99
273_V	307_G	0.975	0.99
336_G	343_F	0.973	0.99
66_V	70_Q	0.972	0.99
85_L	101_R	0.969	0.99
142_G	145_K	0.966	0.99
183_A	196_Y	0.966	0.99
5_N	170_V	0.965	0.99
254_L	258_V	0.962	0.99
130_Y	155_I	0.959	0.99
38_M	79_W	0.954	0.98
28_K	32_E	0.952	0.98
333_H	337_R	0.952	0.98
221_I	239_L	0.951	0.98
327_S	350_K	0.946	0.98
66_V	117_N	0.942	0.98
261_I	268_K	0.938	0.98
131_L	134_F	0.938	0.98
50_G	53_E	0.935	0.98
24_I	27_A	0.935	0.98
48_D	51_I	0.934	0.98
281_I	288_K	0.929	0.98
337_R	341_G	0.923	0.98
49_F	53_E	0.922	0.98
81_S	85_L	0.921	0.98
34_N	79_W	0.921	0.98
247_S	251_K	0.919	0.98
340_R	343_F	0.913	0.98
358_G	361_N	0.906	0.98
7_V	166_A	0.903	0.98
259_R	306_I	0.900	0.98
36_L	40_Q	0.896	0.98
341_G	353_N	0.895	0.98
254_L	295_N	0.893	0.98
74_K	154_E	0.892	0.98
199_D	260_R	0.889	0.98
301_K	304_E	0.887	0.98
140_F	143_R	0.882	0.97
224_V	235_R	0.879	0.97
269_N	362_I	0.878	0.97
187_L	193_A	0.877	0.97
238_R	241_L	0.873	0.97
319_E	322_T	0.871	0.97
220_R	238_R	0.869	0.97
41_Y	79_W	0.867	0.97
196_Y	200_G	0.866	0.97
232_R	236_S	0.865	0.97
158_D	163_I	0.863	0.97
145_K	148_G	0.862	0.97
197_V	265_L	0.860	0.97
245_K	249_F	0.860	0.97
11_V	19_L	0.859	0.97
65_R	118_D	0.858	0.97
41_Y	44_G	0.857	0.97
154_E	167_H	0.857	0.97
30_W	75_N	0.850	0.97
45_K	48_D	0.847	0.97
193_A	257_M	0.846	0.97
262_M	268_K	0.845	0.97
223_I	226_K	0.844	0.97
53_E	73_R	0.843	0.97
189_I	290_T	0.843	0.97
331_E	343_F	0.842	0.97
37_R	76_A	0.840	0.97
336_G	354_A	0.839	0.97
342_L	352_I	0.839	0.97
140_F	144_L	0.838	0.97
72_A	76_A	0.833	0.96
242_L	246_R	0.831	0.96
191_N	200_G	0.828	0.96
37_R	79_W	0.826	0.96
323_S	336_G	0.824	0.96
155_I	166_A	0.823	0.96
82_F	103_P	0.822	0.96
152_R	169_P	0.822	0.96
209_S	249_F	0.822	0.96
366_P	370_G	0.818	0.96
35_W	105_Y	0.817	0.96
275_V	296_Y	0.816	0.96
206_K	294_W	0.815	0.96
217_Y	225_Q	0.809	0.96
221_I	243_H	0.808	0.96
32_E	36_L	0.807	0.96
340_R	354_A	0.806	0.96
356_L	359_A	0.805	0.96
142_G	146_W	0.805	0.96
113_V	116_R	0.802	0.96
325_T	361_N	0.800	0.95
218_S	221_I	0.800	0.95
222_T	225_Q	0.798	0.95
69_Q	73_R	0.797	0.95
365_I	369_R	0.796	0.95
357_N	361_N	0.796	0.95
331_E	347_R	0.794	0.95
114_I	154_E	0.792	0.95
127_R	137_S	0.791	0.95
297_S	301_K	0.791	0.95
321_Y	365_I	0.791	0.95
327_S	358_G	0.791	0.95
307_G	312_I	0.790	0.95
191_N	205_F	0.788	0.95
270_V	316_T	0.787	0.95
127_R	135_N	0.787	0.95
217_Y	246_R	0.785	0.95
44_G	48_D	0.785	0.95
70_Q	74_K	0.781	0.95
368_S	371_S	0.780	0.95
9_L	166_A	0.780	0.95
30_W	78_D	0.779	0.95
194_T	204_L	0.778	0.95
157_Y	164_W	0.778	0.95
70_Q	114_I	0.774	0.95
81_S	84_S	0.774	0.95
16_H	164_W	0.771	0.94
190_V	194_T	0.769	0.94
338_V	354_A	0.764	0.94
24_I	28_K	0.764	0.94
114_I	152_R	0.764	0.94
335_D	340_R	0.763	0.94
119_R	131_L	0.762	0.94
315_I	366_P	0.757	0.94
33_V	56_V	0.755	0.94
123_D	129_V	0.755	0.94
182_K	267_E	0.751	0.94
236_S	240_K	0.751	0.94
115_I	118_D	0.747	0.94
40_Q	47_V	0.747	0.94
63_V	66_V	0.744	0.93
328_L	360_I	0.743	0.93
268_K	271_G	0.741	0.93
29_C	71_V	0.740	0.93
183_A	273_V	0.738	0.93
327_S	364_H	0.735	0.93
253_A	257_M	0.732	0.93
47_V	50_G	0.731	0.93
367_E	371_S	0.725	0.92
22_L	26_T	0.723	0.92
199_D	204_L	0.722	0.92
25_A	28_K	0.719	0.92
111_L	114_I	0.719	0.92
8_R	165_Y	0.717	0.92
66_V	69_Q	0.715	0.92
6_T	156_I	0.714	0.92
75_N	79_W	0.712	0.92
257_M	260_R	0.712	0.92
79_W	82_F	0.711	0.92
49_F	72_A	0.709	0.92
19_L	113_V	0.704	0.91
269_N	273_V	0.701	0.91
87_R	101_R	0.701	0.91
209_S	213_H	0.699	0.91
204_L	257_M	0.695	0.91
246_R	293_F	0.694	0.91
360_I	363_L	0.693	0.91
51_I	54_K	0.691	0.91
128_V	153_L	0.690	0.91
119_R	122_V	0.689	0.90
217_Y	221_I	0.688	0.90
36_L	39_K	0.686	0.90
25_A	63_V	0.685	0.90
225_Q	239_L	0.685	0.90
287_N	291_V	0.685	0.90
279_K	292_N	0.684	0.90
14_E	17_E	0.681	0.90
324_K	333_H	0.679	0.90
178_K	181_I	0.674	0.89
263_E	267_E	0.673	0.89
233_Q	237_R	0.672	0.89
331_E	334_E	0.670	0.89
21_R	25_A	0.669	0.89
219_K	223_I	0.667	0.89
18_V	22_L	0.663	0.89
343_F	354_A	0.661	0.89
275_V	303_L	0.659	0.88
79_W	83_F	0.658	0.88
284_H	287_N	0.653	0.88
200_G	356_L	0.649	0.88
206_K	209_S	0.648	0.88
211_L	289_L	0.646	0.87
49_F	73_R	0.640	0.87
352_I	357_N	0.640	0.87
192_L	258_V	0.639	0.87
181_I	197_V	0.639	0.87
186_D	355_D	0.635	0.87
149_K	171_E	0.634	0.86
71_V	75_N	0.632	0.86
218_S	225_Q	0.631	0.86
141_K	146_W	0.625	0.86
118_D	121_T	0.625	0.86
321_Y	332_A	0.625	0.86
323_S	337_R	0.624	0.86
333_H	340_R	0.618	0.85
123_D	127_R	0.617	0.85
56_V	72_A	0.617	0.85
19_L	23_G	0.616	0.85
362_I	365_I	0.616	0.85
228_L	232_R	0.616	0.85
321_Y	325_T	0.615	0.85
213_H	242_L	0.615	0.85
223_I	235_R	0.614	0.85
22_L	115_I	0.614	0.85
224_V	227_T	0.613	0.85
302_R	306_I	0.612	0.84
124_E	127_R	0.611	0.84
193_A	204_L	0.611	0.84
196_Y	356_L	0.610	0.84
67_N	113_V	0.610	0.84
192_L	294_W	0.607	0.84
28_K	59_K	0.607	0.84
13_K	17_E	0.607	0.84
50_G	54_K	0.606	0.84
364_H	367_E	0.605	0.84
303_L	314_V	0.605	0.84
84_S	87_R	0.604	0.84
112_I	116_R	0.604	0.84
243_H	250_L	0.602	0.84
180_G	266_R	0.601	0.83
261_I	265_L	0.600	0.83
121_T	124_E	0.598	0.83
127_R	130_Y	0.598	0.83
66_V	119_R	0.597	0.83
282_A	288_K	0.597	0.83
112_I	115_I	0.596	0.83
22_L	113_V	0.595	0.83
338_V	353_N	0.593	0.83
63_V	68_A	0.592	0.82
111_L	115_I	0.591	0.82
18_V	21_R	0.591	0.82
115_I	119_R	0.589	0.82
193_A	299_I	0.589	0.82
232_R	235_R	0.588	0.82
4_S	156_I	0.587	0.82
163_I	248_R	0.586	0.82
46_R	49_F	0.586	0.82
46_R	50_G	0.586	0.82
157_Y	165_Y	0.584	0.82
265_L	273_V	0.584	0.82
25_A	60_Y	0.583	0.82
210_A	213_H	0.583	0.82
37_R	52_T	0.580	0.81
188_G	194_T	0.578	0.81
305_D	309_E	0.577	0.81
120_Y	131_L	0.576	0.81
367_E	370_G	0.576	0.81
182_K	269_N	0.576	0.81
45_K	49_F	0.575	0.81
282_A	285_H	0.574	0.81
329_C	333_H	0.573	0.80
35_W	38_M	0.573	0.80
184_S	196_Y	0.570	0.80
248_R	252_H	0.567	0.80
364_H	369_R	0.567	0.80
82_F	85_L	0.567	0.80
251_K	255_N	0.566	0.80
250_L	293_F	0.565	0.79
307_G	310_L	0.564	0.79
51_I	55_A	0.564	0.79
138_L	141_K	0.563	0.79
6_T	167_H	0.563	0.79
73_R	116_R	0.563	0.79
56_V	69_Q	0.563	0.79
229_H	232_R	0.562	0.79
337_R	343_F	0.558	0.79
192_L	254_L	0.557	0.79
220_R	224_V	0.557	0.79
331_E	335_D	0.557	0.79
325_T	343_F	0.556	0.78
72_A	75_N	0.554	0.78
131_L	136_L	0.554	0.78
53_E	72_A	0.553	0.78
190_V	341_G	0.553	0.78
267_E	313_K	0.551	0.78
248_R	251_K	0.551	0.78
318_D	321_Y	0.550	0.78
181_I	266_R	0.549	0.78
44_G	47_V	0.549	0.78
141_K	145_K	0.548	0.77
195_V	200_G	0.548	0.77
40_Q	43_E	0.547	0.77
332_A	335_D	0.547	0.77
22_L	63_V	0.547	0.77
210_A	214_Y	0.547	0.77
211_L	214_Y	0.545	0.77
228_L	236_S	0.543	0.77
284_H	291_V	0.542	0.77
21_R	24_I	0.540	0.76
280_E	288_K	0.540	0.76
281_I	292_N	0.539	0.76
254_L	257_M	0.537	0.76
281_I	286_G	0.536	0.76
64_L	115_I	0.536	0.76
271_G	274_V	0.535	0.76
4_S	169_P	0.535	0.76
53_E	57_Y	0.533	0.75
130_Y	136_L	0.533	0.75
6_T	251_K	0.533	0.75
332_A	336_G	0.533	0.75
333_H	354_A	0.532	0.75
213_H	217_Y	0.531	0.75
68_A	71_V	0.531	0.75
36_L	52_T	0.531	0.75
108_D	111_L	0.531	0.75
328_L	352_I	0.526	0.75
344_V	350_K	0.526	0.75
174_T	308_E	0.526	0.75
362_I	366_P	0.526	0.75
22_L	64_L	0.525	0.74
295_N	298_Y	0.525	0.74
221_I	224_V	0.525	0.74
33_V	52_T	0.524	0.74
300_I	304_E	0.524	0.74
71_V	74_K	0.524	0.74
130_Y	133_D	0.523	0.74
268_K	273_V	0.522	0.74
262_M	314_V	0.522	0.74
32_E	35_W	0.522	0.74
285_H	292_N	0.521	0.74
69_Q	72_A	0.520	0.74
211_L	290_T	0.516	0.73
243_H	247_S	0.516	0.73
232_R	239_L	0.515	0.73
275_V	291_V	0.515	0.73
223_I	238_R	0.515	0.73
135_N	139_K	0.515	0.73
26_T	113_V	0.513	0.73
29_C	68_A	0.511	0.72
322_T	361_N	0.511	0.72
46_R	52_T	0.510	0.72
41_Y	83_F	0.509	0.72
47_V	52_T	0.509	0.72
219_K	222_T	0.508	0.72
144_L	148_G	0.507	0.72
21_R	63_V	0.506	0.72
325_T	331_E	0.506	0.72
207_G	253_A	0.505	0.72
181_I	265_L	0.503	0.71
137_S	140_F	0.502	0.71
246_R	250_L	0.502	0.71
41_Y	82_F	0.500	0.71
218_S	226_K	0.500	0.71

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1nu0A	1	0.3149	94.6	0.933	Contact Map
1vhxA	2	0.3317	93.8	0.936	Contact Map
1iv0A	1	0.2356	92.9	0.938	Contact Map
1ffkW	1	0.1635	88.1	0.944	Contact Map
4aybP	1	0.1034	86.9	0.945	Contact Map
1vq8Z	1	0.1683	80.2	0.95	Contact Map
1vd4A	1	0.1346	78.9	0.95	Contact Map
1qypA	1	0.1106	78.8	0.95	Contact Map
3j20Y	1	0.1202	77.4	0.951	Contact Map
2k4xA	1	0.1322	73.5	0.952	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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