Q97Y57 - SULSO - GREMLIN Contacts

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Q97Y57 - Transposase ISC1058

UniProt:	Q97Y57	Sequences: MEKSKYKRDWSKYDENVITRYKLMFPFYVFEHWWDLLAEENRNARTKYKAPKEFNEFLAFLHIFLPYRAIEGVLRALEQLKIIPTSLDYSTIWERVRNMNITFPETSDELEVIADATGISTNTGGQYIVAKWGKTRDSKFLKIEIVMDNNEFNVINAEVTSNEVESAVKTVKDLQDKGKKVKKFYGDKAYDANEVYKTGVEVVVPPKKNASTKRHLARRKAVREFRKLGYDRWKEEKGYGVRWRVESLFSAVKRTFGESVRQVLLGK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	267 (256)
Sequences:	312 (185)
Seq/√Len:	11.6

SULSO - Q97Y57 Q97YB8 Q97TV4 Q7LX34 Q97XJ2 Q97VP0 Q97UJ1 Q97WA4 Q97US4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
24_M	27_F	4.566	1.00
41_N	46_T	3.536	0.98
170_T	195_V	3.458	0.98
240_G	243_W	3.317	0.97
17_V	20_R	2.868	0.94
248_L	251_A	2.704	0.92
14_D	89_Y	2.693	0.91
10_W	13_Y	2.607	0.90
252_V	260_V	2.539	0.89
239_Y	246_E	2.493	0.88
156_N	200_V	2.341	0.84
213_K	216_L	2.284	0.83
17_V	21_Y	2.248	0.82
88_D	91_T	2.226	0.81
140_F	144_E	2.163	0.79
202_V	221_A	2.163	0.79
191_D	207_K	2.145	0.78
239_Y	242_R	2.119	0.77
121_T	139_K	2.018	0.74
43_N	46_T	2.004	0.73
51_P	54_F	1.967	0.72
114_A	174_L	1.960	0.72
63_I	83_I	1.954	0.71
242_R	246_E	1.947	0.71
243_W	249_F	1.942	0.71
95_R	233_W	1.913	0.70
65_L	193_N	1.902	0.69
191_D	218_R	1.893	0.69
145_I	249_F	1.893	0.69
58_L	74_L	1.864	0.68
130_A	135_T	1.819	0.66
90_S	232_R	1.801	0.65
218_R	233_W	1.788	0.64
159_V	260_V	1.784	0.64
69_A	160_T	1.770	0.63
119_I	159_V	1.752	0.63
123_T	126_Q	1.742	0.62
206_P	210_A	1.740	0.62
81_K	143_I	1.687	0.59
147_M	245_V	1.674	0.59
169_K	172_K	1.666	0.58
95_R	218_R	1.658	0.58
197_K	252_V	1.657	0.58
92_I	185_Y	1.631	0.57
229_G	233_W	1.627	0.57
146_V	150_N	1.579	0.54
119_I	260_V	1.576	0.54
195_V	213_K	1.560	0.53
161_S	245_V	1.558	0.53
127_Y	140_F	1.547	0.53
15_E	19_T	1.547	0.53
24_M	29_V	1.544	0.53
188_K	207_K	1.526	0.52
122_N	139_K	1.522	0.51
124_G	240_G	1.518	0.51
41_N	47_K	1.517	0.51
113_I	149_N	1.515	0.51
6_Y	114_A	1.513	0.51
246_E	253_K	1.501	0.50
231_D	234_K	1.480	0.49
88_D	202_V	1.473	0.49
55_N	95_R	1.465	0.49
29_V	57_F	1.459	0.48
163_E	225_F	1.445	0.48
125_G	181_V	1.421	0.46
25_F	61_L	1.419	0.46
159_V	252_V	1.417	0.46
114_A	139_K	1.392	0.45
18_I	75_R	1.375	0.44
65_L	149_N	1.375	0.44
5_K	155_I	1.346	0.43
134_K	259_S	1.335	0.42
247_S	250_S	1.320	0.42
242_R	253_K	1.320	0.42
127_Y	144_E	1.307	0.41
144_E	160_T	1.300	0.41
186_G	190_Y	1.271	0.39
244_R	248_L	1.254	0.38
244_R	247_S	1.251	0.38
252_V	256_F	1.251	0.38
66_P	69_A	1.248	0.38
26_P	29_V	1.243	0.38
101_I	106_T	1.228	0.37
234_K	252_V	1.207	0.36
52_K	55_N	1.199	0.36
111_E	249_F	1.194	0.36
111_E	149_N	1.193	0.36
124_G	128_I	1.192	0.36
192_A	195_V	1.187	0.35
73_V	210_A	1.187	0.35
248_L	256_F	1.185	0.35
93_W	145_I	1.183	0.35
185_Y	203_V	1.181	0.35
120_S	132_W	1.171	0.35
208_K	211_S	1.171	0.35
46_T	250_S	1.165	0.34
12_K	31_E	1.163	0.34
77_L	102_T	1.162	0.34
144_E	166_S	1.160	0.34
122_N	136_R	1.158	0.34
112_V	155_I	1.158	0.34
152_F	251_A	1.156	0.34
119_I	251_A	1.155	0.34
77_L	155_I	1.146	0.34
42_R	46_T	1.137	0.33
67_Y	80_L	1.136	0.33
11_S	15_E	1.124	0.33
28_Y	200_V	1.122	0.33
132_W	140_F	1.119	0.32
17_V	191_D	1.117	0.32
71_E	89_Y	1.102	0.32
56_E	104_P	1.101	0.32
142_K	166_S	1.100	0.32
57_F	260_V	1.097	0.31
130_A	136_R	1.090	0.31
184_F	200_V	1.090	0.31
125_G	207_K	1.086	0.31
22_K	51_P	1.082	0.31
13_Y	115_D	1.077	0.31
19_T	90_S	1.076	0.31
234_K	240_G	1.062	0.30
50_A	121_T	1.054	0.30
5_K	14_D	1.053	0.30
199_G	224_E	1.051	0.30
231_D	237_K	1.051	0.30
23_L	108_D	1.050	0.29
51_P	59_A	1.043	0.29
70_I	73_V	1.038	0.29
99_M	241_V	1.035	0.29
11_S	29_V	1.025	0.28
52_K	95_R	1.016	0.28
152_F	248_L	1.011	0.28
148_D	189_A	1.009	0.28
140_F	205_P	1.008	0.28
139_K	162_N	1.007	0.28
86_S	133_G	1.006	0.28
94_E	160_T	1.001	0.28
90_S	164_V	0.998	0.27
47_K	196_Y	0.987	0.27
184_F	195_V	0.986	0.27
137_D	141_L	0.984	0.27
33_W	54_F	0.984	0.27
26_P	30_F	0.980	0.27
83_I	117_T	0.977	0.27
111_E	124_G	0.973	0.26
114_A	170_T	0.970	0.26
8_R	18_I	0.970	0.26
213_K	249_F	0.968	0.26
161_S	164_V	0.957	0.26
239_Y	253_K	0.956	0.26
24_M	206_P	0.955	0.26
83_I	154_V	0.954	0.26
12_K	216_L	0.942	0.25
120_S	126_Q	0.927	0.25
187_D	190_Y	0.922	0.25
56_E	96_V	0.921	0.24
93_W	214_R	0.914	0.24
45_R	144_E	0.913	0.24
184_F	202_V	0.911	0.24
61_L	136_R	0.909	0.24
90_S	207_K	0.905	0.24
13_Y	17_V	0.898	0.24
45_R	55_N	0.895	0.24
32_H	36_L	0.893	0.23
170_T	249_F	0.890	0.23
72_G	95_R	0.889	0.23
20_R	33_W	0.886	0.23
73_V	97_R	0.881	0.23
92_I	212_T	0.879	0.23
62_H	202_V	0.877	0.23
128_I	234_K	0.877	0.23
102_T	235_E	0.873	0.23
74_L	261_R	0.866	0.23
92_I	175_Q	0.866	0.23
116_A	166_S	0.865	0.22
16_N	62_H	0.863	0.22
71_E	75_R	0.860	0.22
167_A	259_S	0.859	0.22
81_K	197_K	0.855	0.22
174_L	262_Q	0.851	0.22
89_Y	180_K	0.849	0.22
128_I	135_T	0.848	0.22
13_Y	20_R	0.846	0.22
175_Q	194_E	0.845	0.22
76_A	127_Y	0.841	0.22
49_K	154_V	0.839	0.22
44_A	165_E	0.837	0.22
109_E	160_T	0.832	0.21
15_E	91_T	0.829	0.21
18_I	71_E	0.826	0.21
72_G	206_P	0.826	0.21
145_I	183_K	0.825	0.21
224_E	236_E	0.820	0.21
109_E	180_K	0.819	0.21
100_N	202_V	0.815	0.21
165_E	168_V	0.809	0.21
236_E	240_G	0.809	0.21
80_L	124_G	0.805	0.20
95_R	191_D	0.801	0.20
99_M	107_S	0.799	0.20
115_D	131_K	0.798	0.20
206_P	221_A	0.797	0.20
145_I	189_A	0.794	0.20
141_L	251_A	0.792	0.20
72_G	126_Q	0.791	0.20
123_T	251_A	0.789	0.20
153_N	165_E	0.789	0.20
181_V	256_F	0.787	0.20
35_D	259_S	0.787	0.20
128_I	244_R	0.785	0.20
131_K	144_E	0.779	0.20
92_I	227_K	0.778	0.20
94_E	177_K	0.773	0.19
152_F	256_F	0.773	0.19
19_T	197_K	0.772	0.19
251_A	260_V	0.771	0.19
59_A	67_Y	0.765	0.19
206_P	218_R	0.764	0.19
151_E	240_G	0.756	0.19
212_T	222_V	0.756	0.19
25_F	220_K	0.755	0.19
63_I	157_A	0.754	0.19
8_R	58_L	0.753	0.19
136_R	202_V	0.753	0.19
146_V	162_N	0.749	0.19
129_V	135_T	0.747	0.19
168_V	221_A	0.746	0.19
241_V	247_S	0.745	0.19
72_G	125_G	0.742	0.19
213_K	240_G	0.741	0.19
100_N	163_E	0.741	0.19
81_K	255_T	0.734	0.18
33_W	218_R	0.733	0.18
16_N	32_H	0.732	0.18
86_S	230_Y	0.730	0.18
93_W	111_E	0.729	0.18
116_A	144_E	0.728	0.18
26_P	56_E	0.725	0.18
72_G	144_E	0.725	0.18
206_P	251_A	0.723	0.18
73_V	206_P	0.721	0.18
29_V	32_H	0.720	0.18
200_V	244_R	0.719	0.18
11_S	19_T	0.716	0.18
156_N	173_D	0.714	0.18
166_S	187_D	0.713	0.18
52_K	145_I	0.712	0.18
136_R	240_G	0.711	0.18
135_T	208_K	0.707	0.18
28_Y	58_L	0.707	0.18
66_P	262_Q	0.706	0.17
115_D	190_Y	0.704	0.17
148_D	243_W	0.703	0.17
148_D	207_K	0.701	0.17
247_S	251_A	0.692	0.17
223_R	226_R	0.690	0.17
115_D	118_G	0.689	0.17
162_N	208_K	0.687	0.17
227_K	264_L	0.684	0.17
19_T	30_F	0.683	0.17
45_R	218_R	0.677	0.17
82_I	92_I	0.675	0.17
29_V	258_E	0.675	0.17
30_F	92_I	0.668	0.16
15_E	25_F	0.662	0.16
116_A	160_T	0.661	0.16
107_S	262_Q	0.661	0.16
15_E	200_V	0.660	0.16
172_K	208_K	0.659	0.16
72_G	88_D	0.659	0.16
242_R	257_G	0.659	0.16
153_N	158_E	0.657	0.16
61_L	167_A	0.655	0.16
143_I	206_P	0.653	0.16
251_A	256_F	0.650	0.16
175_Q	180_K	0.650	0.16
123_T	249_F	0.647	0.16
99_M	134_K	0.646	0.16
128_I	185_Y	0.645	0.16
90_S	150_N	0.644	0.16
123_T	154_V	0.641	0.16
85_T	89_Y	0.641	0.16
48_Y	90_S	0.639	0.16
9_D	21_Y	0.637	0.16
58_L	248_L	0.636	0.16
55_N	126_Q	0.635	0.16
253_K	262_Q	0.634	0.16
17_V	187_D	0.633	0.16
7_K	11_S	0.633	0.16
134_K	138_S	0.630	0.15
130_A	213_K	0.627	0.15
41_N	202_V	0.625	0.15
128_I	216_L	0.624	0.15
148_D	203_V	0.617	0.15
114_A	161_S	0.617	0.15
167_A	262_Q	0.615	0.15
46_T	127_Y	0.612	0.15
65_L	174_L	0.611	0.15
28_Y	100_N	0.609	0.15
140_F	190_Y	0.607	0.15
9_D	13_Y	0.607	0.15
106_T	230_Y	0.606	0.15
62_H	145_I	0.606	0.15
23_L	168_V	0.606	0.15
185_Y	216_L	0.605	0.15
132_W	195_V	0.604	0.15
176_D	263_V	0.600	0.15
31_E	34_W	0.600	0.15
123_T	132_W	0.593	0.15
67_Y	250_S	0.593	0.15
12_K	100_N	0.592	0.14
245_V	259_S	0.592	0.14
138_S	233_W	0.591	0.14
149_N	189_A	0.589	0.14
49_K	152_F	0.587	0.14
187_D	204_V	0.587	0.14
193_N	219_R	0.587	0.14
120_S	247_S	0.584	0.14
66_P	238_G	0.582	0.14
154_V	235_E	0.579	0.14
133_G	141_L	0.578	0.14
99_M	145_I	0.578	0.14
257_G	262_Q	0.578	0.14
232_R	240_G	0.577	0.14
35_D	47_K	0.577	0.14
33_W	229_G	0.573	0.14
119_I	261_R	0.571	0.14
30_F	34_W	0.567	0.14
248_L	255_T	0.567	0.14
253_K	257_G	0.566	0.14
76_A	95_R	0.565	0.14
95_R	126_Q	0.565	0.14
65_L	73_V	0.564	0.14
66_P	99_M	0.563	0.14
92_I	194_E	0.563	0.14
33_W	95_R	0.563	0.14
143_I	159_V	0.562	0.14
117_T	245_V	0.561	0.14
72_G	112_V	0.561	0.14
61_L	65_L	0.560	0.14
19_T	22_K	0.560	0.14
113_I	185_Y	0.558	0.14
47_K	169_K	0.555	0.14
56_E	90_S	0.554	0.14
25_F	264_L	0.553	0.14
45_R	95_R	0.553	0.14
24_M	33_W	0.553	0.14
59_A	96_V	0.552	0.14
10_W	133_G	0.551	0.14
206_P	260_V	0.546	0.13
20_R	218_R	0.544	0.13
8_R	37_L	0.543	0.13
121_T	127_Y	0.540	0.13
67_Y	93_W	0.539	0.13
35_D	92_I	0.538	0.13
198_T	207_K	0.538	0.13
62_H	249_F	0.538	0.13
14_D	32_H	0.536	0.13
110_L	186_G	0.533	0.13
25_F	34_W	0.533	0.13
47_K	183_K	0.532	0.13
21_Y	45_R	0.530	0.13
16_N	117_T	0.530	0.13
205_P	260_V	0.527	0.13
62_H	219_R	0.526	0.13
86_S	141_L	0.523	0.13
158_E	165_E	0.522	0.13
11_S	25_F	0.521	0.13
100_N	117_T	0.521	0.13
8_R	215_H	0.520	0.13
108_D	199_G	0.520	0.13
92_I	161_S	0.518	0.13
16_N	162_N	0.516	0.13
55_N	140_F	0.515	0.13
123_T	141_L	0.514	0.13
104_P	222_V	0.510	0.13
228_L	232_R	0.509	0.13
231_D	236_E	0.509	0.13
19_T	37_L	0.508	0.13
14_D	90_S	0.507	0.13
58_L	92_I	0.505	0.12
112_V	126_Q	0.504	0.12
72_G	140_F	0.501	0.12
159_V	224_E	0.500	0.12

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1musA	2	0.8689	92	0.943	Contact Map
4fcyA	4	0.8801	71.7	0.956	Contact Map
4fw1A	2	0.4419	46.2	0.962	Contact Map
4u7bA	2	0.824	32.6	0.965	Contact Map
1bcoA	1	0.5206	30.8	0.966	Contact Map
1cxqA	2	0.4307	30.1	0.966	Contact Map
2gloA	1	0.221	25.2	0.967	Contact Map
3l3uA	2	0.412	16.2	0.97	Contact Map
2cobA	1	0.2584	12.2	0.971	Contact Map
4fw2A	2	0.4195	11.9	0.972	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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