Q97Y48 - SULSO - GREMLIN Contacts

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Q97Y48 - Second ORF in transposon ISC1904

UniProt:	Q97Y48	Sequences: MLKNLRIRKFEPEEKYAYFTYSIKNDKREKSKELIKEYRTLLQKAIDYLWNLTKIQVRKNGNYKITLPKKREVYKPLREELEKINHLASHYVDKAINDAFSILKSWRKRAIKGRASIGKPRVKKAYVRIKTTLRKVVGESVRITVRPYEYITFSWSKSWFSRRVRELELGEPVIKEEKVYLSFRYKLPWATPVNFLAIDSNLYTLDAYDGEKFVTISLKQLYSLKYSMEVKRAKVQSFASKHTKRGRELLRKYSHRERNRVLDFVHKFVNALLDLYPMTFFAVEKLDKEGMFVDANESLSRKISLTVWGSIHRVLRYKAPLYGSFVKEVNPRFTSRSCPRCGFVSRKVGKTFECERCGFKLDRQLNASLNIYLKMCGFPHIREGGVRGYSANGAERDERP	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	400 (343)
Sequences:	4512 (2881)
Seq/√Len:	155.6

ECOLI - INSQ
HALSA - Q9HSW9 Q9HHJ9 Q9HM87 Q9HHG8 Q9HSW7 Q9HSZ6 Q9HSD4 Q9HHU6 Q9HSY4 Q9HHX8
SULSO - Q97Y48 Q97YI5 Q97Z61 Q97U51 Q97XQ0 Q97YU1 Q97XB6 Q97U68 Q97UQ8 Q97YW9 Q97XP1 Q97V47 Q97Y44 Q97XQ8 Q97V25 Q97YI2 Q97YW4 Q97WZ3 Q97Y67 Q97X39 Q7LXD9 Q97WY6 Q97XA4 Q7LXH2 Q97V53 Q7LXH6 Q97U09 Q97YF7 Y2105 Q7LX59 Q97YK6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
263_D	267_K	5.356	1.00
287_D	290_G	4.381	1.00
339_P	366_N	4.300	1.00
319_A	326_V	3.657	1.00
282_A	375_M	3.082	1.00
205_L	311_I	3.023	1.00
283_V	315_L	2.973	1.00
19_F	321_L	2.948	1.00
312_H	328_E	2.946	1.00
346_R	349_G	2.830	1.00
310_S	314_V	2.710	1.00
270_N	274_D	2.679	1.00
336_R	345_S	2.468	1.00
310_S	313_R	2.459	1.00
333_F	336_R	2.437	1.00
172_P	181_L	2.412	1.00
311_I	315_L	2.378	1.00
337_S	344_V	2.343	1.00
316_R	328_E	2.336	1.00
273_L	319_A	2.320	1.00
225_K	301_R	2.277	1.00
329_V	371_I	2.218	1.00
292_F	304_S	2.183	1.00
266_H	317_Y	2.154	1.00
170_G	186_K	2.144	1.00
281_F	319_A	2.135	1.00
334_T	371_I	2.117	1.00
374_K	377_G	2.111	1.00
231_K	246_G	2.067	1.00
341_C	357_C	2.042	1.00
266_H	314_V	2.021	1.00
224_L	260_R	2.018	1.00
296_N	300_S	2.006	1.00
28_R	31_S	1.959	1.00
283_V	326_V	1.930	1.00
205_L	264_F	1.924	1.00
54_K	119_K	1.921	1.00
353_E	360_K	1.921	1.00
36_K	179_V	1.888	1.00
350_K	363_R	1.887	1.00
338_C	341_C	1.885	1.00
49_L	96_I	1.872	1.00
266_H	318_K	1.872	1.00
341_C	354_C	1.869	1.00
269_V	314_V	1.862	1.00
262_L	310_S	1.839	1.00
246_G	250_L	1.834	1.00
297_E	300_S	1.820	1.00
354_C	357_C	1.810	1.00
338_C	357_C	1.805	1.00
316_R	320_P	1.782	1.00
352_F	361_L	1.778	1.00
266_H	269_V	1.770	1.00
265_V	314_V	1.748	1.00
37_E	86_H	1.737	1.00
50_W	99_A	1.717	1.00
340_R	359_F	1.699	1.00
345_S	349_G	1.677	1.00
51_N	55_I	1.654	1.00
265_V	310_S	1.652	1.00
338_C	354_C	1.647	1.00
143_I	181_L	1.642	1.00
35_I	179_V	1.642	1.00
241_K	244_K	1.616	1.00
283_V	328_E	1.583	1.00
244_K	247_R	1.573	1.00
198_I	206_D	1.556	1.00
285_K	328_E	1.519	1.00
284_E	331_P	1.518	1.00
231_K	234_K	1.511	1.00
178_K	255_H	1.504	1.00
44_K	83_K	1.502	1.00
277_P	324_S	1.494	1.00
176_E	255_H	1.468	1.00
207_A	365_L	1.460	1.00
20_T	182_S	1.457	1.00
283_V	312_H	1.455	1.00
269_V	317_Y	1.449	1.00
33_E	37_E	1.445	1.00
352_F	359_F	1.444	1.00
39_R	179_V	1.443	1.00
269_V	318_K	1.395	1.00
316_R	326_V	1.386	1.00
347_K	350_K	1.384	1.00
195_F	372_Y	1.375	1.00
68_P	71_R	1.372	1.00
239_A	243_T	1.371	1.00
67_L	71_R	1.370	1.00
109_R	117_I	1.365	1.00
343_F	355_E	1.355	1.00
49_L	99_A	1.353	1.00
105_S	117_I	1.350	1.00
369_L	373_L	1.349	1.00
208_Y	212_K	1.344	1.00
351_T	360_K	1.344	1.00
32_K	36_K	1.342	1.00
204_T	207_A	1.338	1.00
24_K	178_K	1.330	1.00
100_F	104_K	1.323	1.00
110_A	117_I	1.319	1.00
235_V	247_R	1.312	1.00
144_T	150_Y	1.312	1.00
103_L	119_K	1.305	1.00
196_L	208_Y	1.291	1.00
69_K	72_E	1.281	1.00
133_L	146_R	1.275	1.00
90_H	130_K	1.275	1.00
216_I	219_K	1.273	1.00
255_H	259_N	1.269	1.00
303_I	308_W	1.268	1.00
346_R	350_K	1.263	1.00
228_M	250_L	1.238	1.00
269_V	311_I	1.216	1.00
104_K	108_K	1.201	1.00
265_V	311_I	1.200	1.00
101_S	105_S	1.191	1.00
231_K	253_Y	1.186	1.00
54_K	58_R	1.186	1.00
224_L	257_E	1.181	1.00
196_L	276_Y	1.177	1.00
346_R	363_R	1.171	1.00
90_H	132_T	1.159	1.00
339_P	369_L	1.156	1.00
228_M	254_S	1.153	1.00
347_K	363_R	1.151	1.00
78_R	92_V	1.142	1.00
195_F	280_F	1.140	1.00
271_A	275_L	1.137	1.00
269_V	315_L	1.131	1.00
98_D	128_R	1.128	1.00
273_L	322_Y	1.124	1.00
314_V	317_Y	1.124	1.00
200_S	308_W	1.122	1.00
56_Q	60_N	1.105	0.99
94_K	97_N	1.105	0.99
42_L	95_A	1.103	0.99
145_V	174_I	1.101	0.99
365_L	369_L	1.091	0.99
345_S	352_F	1.089	0.99
287_D	331_P	1.087	0.99
29_E	32_K	1.082	0.99
318_K	321_L	1.071	0.99
109_R	118_G	1.069	0.99
227_S	231_K	1.060	0.99
46_I	95_A	1.056	0.99
232_R	236_Q	1.056	0.99
334_T	374_K	1.054	0.99
198_I	208_Y	1.054	0.99
361_L	369_L	1.053	0.99
288_K	309_G	1.052	0.99
329_V	374_K	1.048	0.99
262_L	314_V	1.045	0.99
88_A	91_Y	1.041	0.99
265_V	269_V	1.031	0.99
337_S	374_K	1.030	0.99
197_A	372_Y	1.027	0.99
137_V	140_S	1.022	0.99
69_K	74_Y	1.019	0.99
21_Y	185_Y	1.017	0.99
105_S	110_A	1.016	0.99
213_F	362_D	1.013	0.99
202_L	306_T	1.006	0.99
339_P	361_L	1.004	0.99
135_K	144_T	1.000	0.99
65_I	69_K	0.998	0.99
106_W	119_K	0.998	0.99
155_W	158_S	0.997	0.99
313_R	316_R	0.994	0.99
235_V	250_L	0.993	0.99
232_R	250_L	0.993	0.99
231_K	250_L	0.986	0.99
55_I	59_K	0.986	0.99
336_R	344_V	0.985	0.99
105_S	109_R	0.983	0.99
273_L	324_S	0.983	0.99
274_D	322_Y	0.981	0.99
127_V	130_K	0.980	0.99
27_K	31_S	0.975	0.99
41_L	83_K	0.974	0.99
127_V	131_T	0.972	0.99
50_W	103_L	0.971	0.99
195_F	376_C	0.968	0.99
74_Y	77_L	0.967	0.99
40_T	43_Q	0.967	0.99
270_N	322_Y	0.965	0.99
207_A	213_F	0.964	0.99
128_R	171_E	0.959	0.99
229_E	232_R	0.959	0.99
200_S	312_H	0.958	0.99
339_P	359_F	0.951	0.98
243_T	247_R	0.947	0.98
273_L	281_F	0.945	0.98
350_K	362_D	0.943	0.98
361_L	365_L	0.941	0.98
105_S	108_K	0.936	0.98
285_K	331_P	0.936	0.98
57_V	61_G	0.935	0.98
296_N	299_L	0.931	0.98
72_E	75_K	0.930	0.98
204_T	215_T	0.925	0.98
329_V	375_M	0.920	0.98
212_K	271_A	0.918	0.98
206_D	268_F	0.917	0.98
258_R	262_L	0.916	0.98
343_F	356_R	0.914	0.98
98_D	173_V	0.913	0.98
192_P	277_P	0.912	0.98
32_K	179_V	0.907	0.98
229_E	233_A	0.906	0.98
339_P	367_A	0.905	0.98
234_K	238_F	0.904	0.98
76_P	80_E	0.903	0.98
231_K	249_L	0.900	0.98
18_Y	186_K	0.898	0.98
349_G	363_R	0.896	0.98
84_I	92_V	0.893	0.98
46_I	102_I	0.893	0.98
282_A	371_I	0.893	0.98
280_F	325_F	0.889	0.98
129_I	145_V	0.886	0.98
70_K	74_Y	0.884	0.98
90_H	94_K	0.882	0.97
228_M	257_E	0.879	0.97
106_W	120_P	0.879	0.97
44_K	48_Y	0.879	0.97
228_M	236_Q	0.879	0.97
365_L	368_S	0.878	0.97
272_L	281_F	0.876	0.97
200_S	206_D	0.875	0.97
54_K	103_L	0.874	0.97
53_T	100_F	0.874	0.97
220_Q	260_R	0.870	0.97
270_N	318_K	0.868	0.97
18_Y	184_R	0.867	0.97
195_F	282_A	0.864	0.97
90_H	131_T	0.862	0.97
196_L	209_D	0.862	0.97
27_K	35_I	0.861	0.97
52_L	73_V	0.861	0.97
94_K	128_R	0.861	0.97
292_F	296_N	0.860	0.97
46_I	99_A	0.858	0.97
352_F	363_R	0.857	0.97
345_S	363_R	0.856	0.97
319_A	324_S	0.856	0.97
253_Y	257_E	0.852	0.97
351_T	361_L	0.852	0.97
339_P	373_L	0.851	0.97
340_R	369_L	0.851	0.97
265_V	307_V	0.850	0.97
58_R	61_G	0.846	0.97
93_D	97_N	0.839	0.97
367_A	370_N	0.838	0.97
256_R	260_R	0.834	0.96
75_K	78_R	0.831	0.96
75_K	79_E	0.828	0.96
214_V	264_F	0.827	0.96
68_P	72_E	0.826	0.96
70_K	73_V	0.825	0.96
235_V	246_G	0.824	0.96
202_L	302_K	0.823	0.96
366_N	370_N	0.823	0.96
94_K	98_D	0.815	0.96
249_L	252_K	0.814	0.96
374_K	378_F	0.814	0.96
230_V	234_K	0.807	0.96
153_F	156_S	0.806	0.96
71_R	75_K	0.804	0.96
331_P	334_T	0.804	0.96
240_S	243_T	0.803	0.96
290_G	331_P	0.802	0.96
236_Q	250_L	0.800	0.96
173_V	182_S	0.800	0.96
288_K	308_W	0.799	0.96
333_F	374_K	0.796	0.95
60_N	63_Y	0.791	0.95
220_Q	224_L	0.787	0.95
232_R	254_S	0.785	0.95
280_F	327_K	0.783	0.95
292_F	300_S	0.780	0.95
194_N	210_G	0.778	0.95
343_F	352_F	0.778	0.95
309_G	313_R	0.773	0.95
203_Y	207_A	0.772	0.95
142_R	150_Y	0.771	0.95
247_R	251_R	0.768	0.94
199_D	364_Q	0.767	0.94
23_I	181_L	0.767	0.94
285_K	312_H	0.765	0.94
286_L	331_P	0.761	0.94
48_Y	52_L	0.760	0.94
57_V	103_L	0.756	0.94
369_L	372_Y	0.755	0.94
268_F	271_A	0.751	0.94
70_K	97_N	0.750	0.94
355_E	363_R	0.745	0.94
204_T	213_F	0.745	0.94
337_S	370_N	0.744	0.93
201_N	207_A	0.741	0.93
65_I	68_P	0.740	0.93
299_L	303_I	0.738	0.93
282_A	327_K	0.737	0.93
96_I	100_F	0.735	0.93
327_K	375_M	0.734	0.93
133_L	136_V	0.733	0.93
87_L	90_H	0.731	0.93
290_G	303_I	0.730	0.93
24_K	180_Y	0.726	0.93
205_L	306_T	0.719	0.92
34_L	38_Y	0.719	0.92
135_K	138_G	0.716	0.92
282_A	372_Y	0.716	0.92
53_T	103_L	0.716	0.92
25_N	181_L	0.715	0.92
68_P	74_Y	0.714	0.92
191_T	194_N	0.712	0.92
264_F	268_F	0.712	0.92
196_L	281_F	0.711	0.92
134_R	169_L	0.711	0.92
73_V	96_I	0.711	0.92
272_L	276_Y	0.707	0.92
90_H	93_D	0.705	0.92
168_E	186_K	0.704	0.91
38_Y	42_L	0.704	0.91
41_L	87_L	0.701	0.91
234_K	246_G	0.697	0.91
297_E	304_S	0.695	0.91
231_K	235_V	0.695	0.91
94_K	130_K	0.694	0.91
29_E	33_E	0.693	0.91
213_F	365_L	0.693	0.91
45_A	95_A	0.688	0.91
345_S	350_K	0.683	0.90
40_T	44_K	0.683	0.90
243_T	250_L	0.681	0.90
361_L	366_N	0.680	0.90
235_V	243_T	0.680	0.90
205_L	269_V	0.679	0.90
103_L	107_R	0.677	0.90
75_K	96_I	0.677	0.90
234_K	237_S	0.674	0.90
333_F	337_S	0.673	0.90
171_E	184_R	0.671	0.89
39_R	127_V	0.670	0.89
216_I	306_T	0.669	0.89
261_V	305_L	0.668	0.89
189_W	324_S	0.667	0.89
137_V	144_T	0.667	0.89
51_N	122_V	0.666	0.89
317_Y	321_L	0.666	0.89
174_I	181_L	0.666	0.89
23_I	157_K	0.665	0.89
257_E	305_L	0.664	0.89
102_I	120_P	0.663	0.89
74_Y	78_R	0.663	0.89
103_L	106_W	0.662	0.89
99_A	103_L	0.662	0.89
41_L	44_K	0.661	0.89
89_S	130_K	0.659	0.89
71_R	96_I	0.658	0.88
349_G	352_F	0.658	0.88
74_Y	93_D	0.658	0.88
92_V	95_A	0.657	0.88
144_T	147_P	0.657	0.88
222_Y	302_K	0.654	0.88
206_D	311_I	0.650	0.88
229_E	236_Q	0.649	0.88
235_V	238_F	0.644	0.87
35_I	39_R	0.640	0.87
228_M	232_R	0.640	0.87
189_W	192_P	0.640	0.87
55_I	58_R	0.640	0.87
128_R	173_V	0.637	0.87
52_L	55_I	0.636	0.87
333_F	344_V	0.636	0.87
95_A	99_A	0.636	0.87
41_L	84_I	0.635	0.87
288_K	291_M	0.635	0.87
314_V	318_K	0.633	0.86
90_H	133_L	0.632	0.86
37_E	41_L	0.631	0.86
337_S	342_G	0.631	0.86
76_P	79_E	0.629	0.86
49_L	73_V	0.626	0.86
347_K	362_D	0.625	0.86
66_T	69_K	0.624	0.86
224_L	256_R	0.619	0.85
319_A	322_Y	0.619	0.85
69_K	73_V	0.619	0.85
26_D	154_S	0.618	0.85
315_L	326_V	0.618	0.85
106_W	109_R	0.614	0.85
371_I	374_K	0.614	0.85
130_K	133_L	0.612	0.85
262_L	266_H	0.612	0.85
274_D	280_F	0.611	0.85
292_F	297_E	0.605	0.84
143_I	172_P	0.605	0.84
153_F	157_K	0.603	0.84
347_K	351_T	0.603	0.84
38_Y	88_A	0.601	0.84
30_K	33_E	0.599	0.83
340_R	361_L	0.597	0.83
107_R	110_A	0.595	0.83
281_F	315_L	0.595	0.83
282_A	374_K	0.594	0.83
233_A	236_Q	0.593	0.83
276_Y	281_F	0.592	0.83
62_N	65_I	0.592	0.83
234_K	249_L	0.592	0.83
373_L	378_F	0.591	0.83
207_A	215_T	0.590	0.82
205_L	265_V	0.590	0.82
332_R	336_R	0.590	0.82
91_Y	127_V	0.589	0.82
238_F	246_G	0.586	0.82
221_L	224_L	0.586	0.82
194_N	276_Y	0.586	0.82
224_L	228_M	0.585	0.82
337_S	352_F	0.581	0.81
71_R	74_Y	0.581	0.81
44_K	81_L	0.581	0.81
222_Y	225_K	0.579	0.81
254_S	261_V	0.577	0.81
244_K	248_E	0.577	0.81
48_Y	77_L	0.576	0.81
70_K	77_L	0.575	0.81
27_K	151_I	0.575	0.81
64_K	69_K	0.574	0.81
96_I	99_A	0.574	0.81
145_V	148_Y	0.572	0.80
87_L	92_V	0.572	0.80
89_S	132_T	0.570	0.80
243_T	246_G	0.569	0.80
48_Y	83_K	0.567	0.80
330_N	333_F	0.561	0.79
208_Y	214_V	0.561	0.79
53_T	70_K	0.559	0.79
224_L	253_Y	0.559	0.79
203_Y	350_K	0.556	0.79
38_Y	87_L	0.555	0.78
22_S	182_S	0.554	0.78
21_Y	157_K	0.553	0.78
56_Q	62_N	0.553	0.78
56_Q	61_G	0.553	0.78
52_L	77_L	0.552	0.78
349_G	355_E	0.552	0.78
108_K	117_I	0.551	0.78
132_T	135_K	0.551	0.78
254_S	258_R	0.550	0.78
73_V	93_D	0.547	0.77
70_K	93_D	0.546	0.77
267_K	270_N	0.545	0.77
27_K	32_K	0.545	0.77
20_T	184_R	0.545	0.77
126_Y	129_I	0.544	0.77
34_L	37_E	0.544	0.77
345_S	348_V	0.544	0.77
73_V	77_L	0.543	0.77
37_E	40_T	0.543	0.77
222_Y	301_R	0.543	0.77
209_D	365_L	0.541	0.77
199_D	335_S	0.540	0.77
38_Y	129_I	0.540	0.77
288_K	303_I	0.539	0.76
202_L	293_V	0.537	0.76
242_H	245_R	0.536	0.76
290_G	293_V	0.535	0.76
61_G	64_K	0.534	0.76
336_R	346_R	0.534	0.76
238_F	242_H	0.532	0.76
203_Y	346_R	0.532	0.76
148_Y	151_I	0.531	0.75
95_A	98_D	0.530	0.75
293_V	296_N	0.529	0.75
74_Y	96_I	0.528	0.75
309_G	312_H	0.527	0.75
146_R	151_I	0.526	0.75
134_R	140_S	0.526	0.75
126_Y	130_K	0.524	0.74
67_L	72_E	0.522	0.74
91_Y	129_I	0.522	0.74
200_S	283_V	0.518	0.74
178_K	259_N	0.515	0.73
259_N	263_D	0.515	0.73
218_L	306_T	0.515	0.73
214_V	268_F	0.514	0.73
312_H	316_R	0.514	0.73
52_L	56_Q	0.513	0.73
220_Q	305_L	0.513	0.73
57_V	107_R	0.513	0.73
22_S	262_L	0.513	0.73
259_N	262_L	0.512	0.73
38_Y	41_L	0.511	0.73
270_N	314_V	0.510	0.72
45_A	92_V	0.510	0.72
299_L	302_K	0.509	0.72
364_Q	367_A	0.509	0.72
145_V	151_I	0.508	0.72
202_L	292_F	0.506	0.72
197_A	282_A	0.506	0.72
66_T	97_N	0.506	0.72
229_E	301_R	0.505	0.72
229_E	237_S	0.505	0.72
333_F	352_F	0.504	0.72
265_V	268_F	0.503	0.71
235_V	239_A	0.502	0.71
18_Y	170_G	0.502	0.71
39_R	42_L	0.502	0.71
37_E	87_L	0.501	0.71
300_S	304_S	0.501	0.71
52_L	72_E	0.500	0.71
341_C	344_V	0.500	0.71
359_F	365_L	0.500	0.71

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1nu0A	1	0.325	95.4	0.93	Contact Map
1vhxA	2	0.345	95.3	0.93	Contact Map
1iv0A	1	0.2425	93	0.937	Contact Map
4aybP	1	0.1	84.8	0.947	Contact Map
1qypA	1	0.1075	81.2	0.949	Contact Map
2k4xA	1	0.1325	79.6	0.95	Contact Map
1ffkW	1	0.1525	79.2	0.95	Contact Map
3j20Y	1	0.12	77.9	0.95	Contact Map
4bbrM	1	0.1625	73.9	0.952	Contact Map
1vq8Z	1	0.1775	73	0.952	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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