Q97Y17 - SULSO - GREMLIN Contacts

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Q97Y17 - Uncharacterized protein

UniProt:	Q97Y17	Sequences: MVENKIKPELFWVMDYYSESGLTKREYYEQVAKEIEFAEKLGYRGVWIAEHHFIDYGIVPSTSVYLSHIASRTKNIRIGPAVATIVFKNPIQVAEEYSLLDTLSNGRLNLAMGSGYLKHEFAGFNIPQEAKREIFDEALDIILKLMSGESVSVNGKYFKYENIKINVLPLQKPYPPIWIAVLRAEAAYHVGKKGFNLMMIPYATVDKIDDVKPVTDSYKQGLKENTNAEISLAFHTHISKSFHDAVEESKEYLHRYVFSRLYAKRRTLDDLYNSGLLLLGGPEDVANQIYKIWKLTEVNRLMFLVDYGMKPLALIQDILINIREKVAKLLEERGIELDFIT	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	341 (292)
Sequences:	16548 (10396)
Seq/√Len:	608.4

ECOLI - RUTA YHBW SSUD
BACSU - SSUD YXEK YDDN MOXC YTMO YVBT YWCH YCEB
HALSA - Q9HQZ9
SULSO - Q97Y17

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
242_F	284_D	4.819	1.00
154_N	159_K	3.282	1.00
36_E	72_R	3.231	1.00
37_F	320_I	2.722	1.00
77_R	109_N	2.588	1.00
242_F	281_G	2.485	1.00
37_F	40_K	2.441	1.00
136_D	192_K	2.433	1.00
39_E	75_N	2.388	1.00
288_Q	291_K	2.377	1.00
216_D	220_Q	2.335	1.00
70_A	104_S	2.286	1.00
66_L	78_I	2.262	1.00
281_G	284_D	2.235	1.00
263_A	266_R	2.228	1.00
133_E	156_K	2.197	1.00
242_F	246_V	2.195	1.00
241_S	244_D	2.167	1.00
140_D	193_K	2.140	1.00
188_Y	221_G	2.134	1.00
290_Y	294_K	2.107	1.00
239_S	244_D	2.061	1.00
35_I	69_I	1.998	1.00
320_I	324_E	1.988	1.00
32_A	36_E	1.982	1.00
322_I	326_V	1.981	1.00
29_E	105_N	1.949	1.00
36_E	40_K	1.948	1.00
217_S	221_G	1.932	1.00
212_K	216_D	1.928	1.00
215_T	230_I	1.917	1.00
240_K	325_K	1.892	1.00
243_H	247_E	1.877	1.00
147_S	174_Y	1.810	1.00
97_Y	108_L	1.797	1.00
41_L	323_R	1.792	1.00
191_G	221_G	1.769	1.00
46_V	76_I	1.766	1.00
33_K	36_E	1.762	1.00
32_A	72_R	1.761	1.00
70_A	78_I	1.752	1.00
47_W	79_G	1.747	1.00
250_K	254_H	1.740	1.00
101_D	106_G	1.729	1.00
222_L	227_N	1.724	1.00
61_S	96_E	1.718	1.00
213_P	216_D	1.708	1.00
317_D	320_I	1.683	1.00
278_L	285_V	1.658	1.00
130_A	156_K	1.638	1.00
222_L	228_A	1.625	1.00
291_K	295_L	1.602	1.00
133_E	157_Y	1.599	1.00
212_K	295_L	1.588	1.00
143_L	174_Y	1.587	1.00
220_Q	224_E	1.586	1.00
215_T	219_K	1.584	1.00
37_F	316_Q	1.561	1.00
97_Y	110_L	1.559	1.00
181_V	197_L	1.536	1.00
35_I	46_V	1.535	1.00
317_D	321_N	1.532	1.00
118_K	122_A	1.524	1.00
37_F	41_L	1.510	1.00
144_K	151_V	1.506	1.00
268_L	272_Y	1.487	1.00
284_D	287_N	1.482	1.00
140_D	144_K	1.477	1.00
152_S	162_N	1.470	1.00
217_S	220_Q	1.466	1.00
69_I	78_I	1.451	1.00
33_K	316_Q	1.445	1.00
101_D	171_Q	1.434	1.00
232_L	298_V	1.430	1.00
221_G	224_E	1.429	1.00
283_E	287_N	1.428	1.00
143_L	193_K	1.401	1.00
152_S	161_E	1.387	1.00
188_Y	192_K	1.382	1.00
286_A	290_Y	1.370	1.00
244_D	248_E	1.358	1.00
32_A	68_H	1.346	1.00
238_I	285_V	1.344	1.00
143_L	177_I	1.343	1.00
23_T	26_E	1.338	1.00
188_Y	217_S	1.335	1.00
40_K	74_K	1.333	1.00
140_D	153_V	1.331	1.00
68_H	71_S	1.325	1.00
321_N	325_K	1.319	1.00
187_A	217_S	1.313	1.00
39_E	73_T	1.309	1.00
184_A	217_S	1.302	1.00
144_K	148_G	1.300	1.00
68_H	103_L	1.299	1.00
144_K	149_E	1.293	1.00
213_P	217_S	1.283	1.00
284_D	288_Q	1.276	1.00
240_K	317_D	1.270	1.00
290_Y	293_W	1.269	1.00
150_S	162_N	1.258	1.00
154_N	161_E	1.252	1.00
48_I	66_L	1.239	1.00
130_A	133_E	1.231	1.00
61_S	64_V	1.227	1.00
136_D	193_K	1.217	1.00
238_I	282_P	1.214	1.00
26_E	30_Q	1.213	1.00
88_K	96_E	1.211	1.00
35_I	73_T	1.209	1.00
29_E	33_K	1.207	1.00
287_N	291_K	1.201	1.00
269_D	273_N	1.189	1.00
188_Y	224_E	1.184	1.00
60_P	99_L	1.181	1.00
291_K	294_K	1.177	1.00
229_E	299_N	1.174	1.00
282_P	321_N	1.172	1.00
39_E	74_K	1.160	1.00
110_L	142_I	1.153	1.00
239_S	245_A	1.150	1.00
33_K	37_F	1.148	1.00
93_V	138_A	1.146	1.00
144_K	153_V	1.145	1.00
244_D	247_E	1.144	1.00
178_W	231_S	1.131	1.00
105_N	170_L	1.125	1.00
221_G	225_N	1.125	1.00
41_L	319_L	1.123	1.00
139_L	193_K	1.121	1.00
247_E	250_K	1.120	1.00
237_H	248_E	1.115	1.00
143_L	195_F	1.114	1.00
136_D	140_D	1.111	1.00
222_L	225_N	1.110	1.00
208_I	295_L	1.108	1.00
79_G	109_N	1.107	1.00
185_E	188_Y	1.105	1.00
178_W	196_N	1.102	1.00
122_A	161_E	1.093	1.00
66_L	80_P	1.086	1.00
141_I	153_V	1.083	1.00
100_L	108_L	1.081	1.00
35_I	76_I	1.076	1.00
48_I	69_I	1.074	1.00
240_K	244_D	1.071	1.00
249_S	253_L	1.060	1.00
63_S	96_E	1.058	1.00
303_F	322_I	1.054	1.00
124_F	127_P	1.051	1.00
142_I	146_M	1.050	1.00
278_L	288_Q	1.042	1.00
247_E	251_E	1.041	1.00
238_I	321_N	1.039	1.00
64_V	67_S	1.038	1.00
270_D	273_N	1.034	1.00
267_T	270_D	1.033	1.00
280_G	285_V	1.030	1.00
68_H	72_R	1.027	1.00
92_Q	96_E	1.023	1.00
130_A	157_Y	1.019	1.00
141_I	158_F	1.016	1.00
269_D	272_Y	1.015	1.00
137_E	155_G	1.005	1.00
134_I	137_E	1.004	1.00
210_D	213_P	0.990	1.00
36_E	74_K	0.988	1.00
191_G	225_N	0.979	1.00
112_M	139_L	0.977	1.00
22_L	30_Q	0.975	1.00
40_K	320_I	0.973	1.00
143_L	147_S	0.972	1.00
246_V	250_K	0.963	1.00
63_S	83_A	0.955	1.00
268_L	273_N	0.950	1.00
236_T	303_F	0.945	1.00
141_I	160_Y	0.942	1.00
234_F	278_L	0.940	1.00
25_R	29_E	0.939	1.00
64_V	100_L	0.938	1.00
267_T	274_S	0.935	1.00
136_D	189_H	0.935	1.00
30_Q	33_K	0.925	1.00
229_E	300_R	0.920	1.00
198_M	231_S	0.916	1.00
102_T	170_L	0.915	1.00
268_L	271_L	0.915	1.00
192_K	225_N	0.912	1.00
189_H	193_K	0.911	1.00
190_V	197_L	0.907	1.00
160_Y	163_I	0.906	1.00
318_I	321_N	0.905	1.00
241_S	245_A	0.905	1.00
43_Y	323_R	0.904	1.00
133_E	185_E	0.904	1.00
243_H	246_V	0.902	1.00
239_S	248_E	0.896	1.00
286_A	326_V	0.890	1.00
141_I	144_K	0.883	1.00
82_V	180_A	0.879	1.00
45_G	79_G	0.877	1.00
21_G	24_K	0.870	1.00
267_T	271_L	0.866	1.00
150_S	164_K	0.864	1.00
153_V	156_K	0.862	1.00
287_N	290_Y	0.858	1.00
38_A	319_L	0.852	1.00
130_A	134_I	0.852	1.00
218_Y	228_A	0.851	1.00
137_E	156_K	0.845	1.00
305_V	322_I	0.843	1.00
116_Y	120_E	0.835	1.00
85_I	138_A	0.835	1.00
33_K	40_K	0.834	1.00
188_Y	225_N	0.832	1.00
257_V	260_R	0.831	1.00
70_A	108_L	0.830	1.00
82_V	113_G	0.814	1.00
29_E	32_A	0.813	1.00
218_Y	222_L	0.812	1.00
28_Y	103_L	0.812	1.00
67_S	71_S	0.810	1.00
146_M	175_P	0.808	1.00
249_S	279_L	0.808	1.00
219_K	230_I	0.806	1.00
45_G	300_R	0.806	1.00
240_K	321_N	0.805	1.00
216_D	297_E	0.804	1.00
187_A	191_G	0.804	1.00
271_L	274_S	0.800	1.00
134_I	138_A	0.799	1.00
219_K	223_K	0.797	1.00
238_I	326_V	0.795	1.00
323_R	327_A	0.794	1.00
319_L	323_R	0.794	1.00
188_Y	220_Q	0.792	1.00
211_V	215_T	0.791	1.00
117_L	120_E	0.789	1.00
40_K	324_E	0.788	1.00
316_Q	320_I	0.784	1.00
66_L	70_A	0.783	1.00
137_E	141_I	0.779	1.00
248_E	321_N	0.774	1.00
258_F	262_Y	0.772	1.00
98_S	169_P	0.761	1.00
234_F	289_I	0.756	1.00
145_L	151_V	0.752	1.00
138_A	142_I	0.751	1.00
251_E	255_R	0.749	1.00
70_A	100_L	0.746	1.00
85_I	93_V	0.745	1.00
66_L	100_L	0.745	1.00
179_I	194_G	0.744	1.00
251_E	254_H	0.740	1.00
90_P	138_A	0.732	1.00
90_P	141_I	0.731	1.00
102_T	173_P	0.730	1.00
71_S	104_S	0.730	1.00
115_G	120_E	0.728	1.00
47_W	81_A	0.728	1.00
132_R	185_E	0.726	1.00
179_I	186_A	0.724	1.00
248_E	251_E	0.721	1.00
223_K	226_T	0.721	1.00
119_H	122_A	0.717	1.00
246_V	249_S	0.712	1.00
27_Y	30_Q	0.712	1.00
211_V	214_V	0.707	1.00
28_Y	68_H	0.705	1.00
187_A	213_P	0.705	1.00
66_L	108_L	0.705	1.00
32_A	69_I	0.705	1.00
254_H	258_F	0.705	1.00
192_K	221_G	0.704	1.00
179_I	190_V	0.704	1.00
50_E	88_K	0.702	1.00
215_T	218_Y	0.702	1.00
140_D	192_K	0.701	1.00
136_D	185_E	0.700	1.00
236_T	322_I	0.699	1.00
224_E	227_N	0.698	1.00
231_S	300_R	0.695	1.00
84_T	115_G	0.694	1.00
283_E	286_A	0.690	1.00
184_A	188_Y	0.690	1.00
255_R	258_F	0.688	1.00
109_N	178_W	0.685	1.00
269_D	274_S	0.684	1.00
305_V	319_L	0.684	1.00
93_V	142_I	0.684	1.00
250_K	253_L	0.683	1.00
52_H	95_E	0.683	1.00
67_S	100_L	0.681	1.00
34_E	319_L	0.681	1.00
233_A	302_M	0.680	1.00
45_G	302_M	0.678	1.00
144_K	147_S	0.677	1.00
219_K	222_L	0.675	1.00
153_V	158_F	0.673	1.00
210_D	214_V	0.672	1.00
214_V	217_S	0.671	1.00
289_I	301_L	0.667	1.00
187_A	197_L	0.665	1.00
293_W	298_V	0.660	1.00
164_K	168_L	0.659	1.00
218_Y	221_G	0.656	1.00
171_Q	175_P	0.655	1.00
129_E	185_E	0.655	1.00
43_Y	327_A	0.655	1.00
79_G	178_W	0.652	1.00
114_S	135_F	0.649	1.00
34_E	316_Q	0.649	1.00
97_Y	146_M	0.648	1.00
179_I	189_H	0.648	1.00
48_I	65_Y	0.646	1.00
88_K	92_Q	0.642	1.00
78_I	104_S	0.641	1.00
29_E	74_K	0.640	1.00
181_V	186_A	0.639	1.00
101_D	169_P	0.638	1.00
253_L	256_Y	0.637	1.00
80_P	108_L	0.635	1.00
30_Q	34_E	0.635	1.00
101_D	174_Y	0.635	1.00
89_N	92_Q	0.632	1.00
140_D	143_L	0.630	1.00
208_I	291_K	0.629	1.00
90_P	158_F	0.627	1.00
223_K	227_N	0.626	1.00
63_S	97_Y	0.625	1.00
222_L	226_T	0.623	1.00
148_G	168_L	0.620	1.00
163_I	167_V	0.619	1.00
101_D	170_L	0.618	1.00
91_I	145_L	0.617	1.00
257_V	261_L	0.617	1.00
171_Q	174_Y	0.616	1.00
77_R	176_P	0.615	1.00
37_F	323_R	0.615	1.00
208_I	212_K	0.614	1.00
44_R	75_N	0.612	1.00
134_I	158_F	0.611	1.00
29_E	71_S	0.604	1.00
32_A	71_S	0.604	1.00
282_P	325_K	0.603	1.00
141_I	151_V	0.601	1.00
32_A	35_I	0.601	1.00
246_V	279_L	0.599	1.00
46_V	69_I	0.598	1.00
230_I	298_V	0.598	1.00
208_I	211_V	0.597	1.00
209_D	212_K	0.596	1.00
88_K	93_V	0.596	1.00
192_K	224_E	0.595	1.00
101_D	104_S	0.595	1.00
26_E	29_E	0.594	1.00
276_L	288_Q	0.594	1.00
187_A	218_Y	0.593	1.00
137_E	140_D	0.593	1.00
137_E	157_Y	0.592	1.00
114_S	131_K	0.589	1.00
239_S	242_F	0.588	1.00
98_S	146_M	0.588	1.00
240_K	282_P	0.588	1.00
214_V	218_Y	0.587	1.00
187_A	221_G	0.587	1.00
289_I	303_F	0.587	1.00
82_V	93_V	0.586	1.00
24_K	27_Y	0.584	1.00
282_P	324_E	0.582	1.00
237_H	249_S	0.581	1.00
44_R	77_R	0.581	1.00
77_R	171_Q	0.580	1.00
270_D	274_S	0.579	1.00
288_Q	292_I	0.578	1.00
259_S	262_Y	0.574	1.00
65_Y	99_L	0.572	1.00
236_T	278_L	0.569	1.00
168_L	171_Q	0.567	1.00
127_P	159_K	0.564	1.00
191_G	222_L	0.563	1.00
203_A	209_D	0.561	1.00
73_T	107_R	0.560	1.00
138_A	158_F	0.559	1.00
139_L	179_I	0.558	1.00
38_A	76_I	0.558	1.00
216_D	219_K	0.557	1.00
22_L	26_E	0.556	1.00
68_H	104_S	0.549	0.99
255_R	259_S	0.549	0.99
177_I	194_G	0.549	0.99
39_E	76_I	0.548	0.99
189_H	192_K	0.548	0.99
64_V	68_H	0.548	0.99
219_K	297_E	0.547	0.99
101_D	175_P	0.547	0.99
111_A	180_A	0.546	0.99
81_A	198_M	0.544	0.99
185_E	189_H	0.543	0.99
166_N	171_Q	0.543	0.99
154_N	157_Y	0.542	0.99
242_F	245_A	0.539	0.99
215_T	298_V	0.536	0.99
28_Y	32_A	0.532	0.99
112_M	142_I	0.531	0.99
90_P	142_I	0.531	0.99
127_P	130_A	0.529	0.99
31_V	34_E	0.527	0.99
67_S	104_S	0.527	0.99
305_V	318_I	0.524	0.99
53_F	117_L	0.524	0.99
240_K	324_E	0.523	0.99
267_T	272_Y	0.522	0.99
73_T	76_I	0.520	0.99
137_E	153_V	0.519	0.99
164_K	167_V	0.518	0.99
197_L	214_V	0.518	0.99
170_L	173_P	0.516	0.99
182_L	200_I	0.513	0.99
139_L	189_H	0.513	0.99
174_Y	195_F	0.512	0.99
176_P	229_E	0.512	0.99
256_Y	260_R	0.512	0.99
85_I	112_M	0.511	0.99
285_V	288_Q	0.511	0.99
241_S	281_G	0.510	0.99
109_N	300_R	0.509	0.99
187_A	214_V	0.509	0.99
213_P	220_Q	0.508	0.99
83_A	93_V	0.507	0.99
258_F	261_L	0.507	0.99
94_A	146_M	0.506	0.99
64_V	99_L	0.506	0.99
62_T	80_P	0.506	0.99
41_L	316_Q	0.505	0.99
139_L	143_L	0.502	0.99
128_Q	131_K	0.501	0.99
146_M	177_I	0.500	0.99

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
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HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1lucA	1	0.9208	100	0.212	Contact Map
2wgkA	2	0.9531	100	0.221	Contact Map
1lucB	1	0.9208	100	0.231	Contact Map
2i7gA	2	0.9589	100	0.235	Contact Map
3sdoA	2	0.9619	100	0.247	Contact Map
3b9oA	2	0.9472	100	0.251	Contact Map
1tvlA	2	0.9267	100	0.257	Contact Map
3raoA	2	0.9413	100	0.257	Contact Map
1ezwA	3	0.8886	100	0.261	Contact Map
3c8nA	2	0.9062	100	0.265	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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