Q97XZ9 - SULSO - GREMLIN Contacts

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Q97XZ9 - First ORF in transposon ISC1491

UniProt:	Q97XZ9	Sequences: MESEIKTRGFAIDVSRDKLTVAKAELVITQEKREVEVNEVREFKYDNEGIEELIKFLGEYKEGIMESTGPYFFYLHEELTEKGYKVTLINPLHLKEVFGKKTDKLDAQRLAKAFILGAVKGSYIPTGEIRELRELTRYRESLMRKITQVKNEIRKFLEMAGYKIEPFDKRGIALLEKLSRGEGLSKEERDELKEKLGRSLNDAEKLALKQLVDLLKSLEAMVKEVEDMIISKIPQPVVELSRELV	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	245 (219)
Sequences:	2976 (2342)
Seq/√Len:	158.3

SULSO - Q97XZ9 Q97XG9 Q97YS4 Q97X07 Q97ZN4 Q97WZ5 Q97UF7 Q97XK2 Q97VS0 Q97WC0 Q97Y59 Q97UU8 Q97X26 Q97XM7 Q97X12 Q97X29 Q97UQ6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
64_I	114_F	5.739	1.00
22_A	107_A	3.518	1.00
176_E	216_K	3.201	1.00
94_L	110_L	3.162	1.00
63_G	84_Y	3.118	1.00
145_K	148_Q	2.902	1.00
18_K	42_E	2.893	1.00
96_E	99_G	2.632	1.00
64_I	110_L	2.606	1.00
21_V	53_L	2.603	1.00
139_R	143_M	2.535	1.00
145_K	152_E	2.471	1.00
146_I	219_E	2.437	1.00
228_M	231_S	2.292	1.00
147_T	151_N	2.244	1.00
10_F	23_K	2.122	1.00
24_A	111_A	2.077	1.00
46_D	49_G	1.973	1.00
12_I	19_L	1.949	1.00
217_S	221_M	1.941	1.00
212_V	216_K	1.884	1.00
12_I	75_L	1.884	1.00
213_D	220_A	1.882	1.00
24_A	115_I	1.874	1.00
78_E	82_K	1.863	1.00
136_T	229_I	1.856	1.00
189_R	193_K	1.820	1.00
210_Q	221_M	1.814	1.00
41_R	56_F	1.777	1.00
65_M	86_V	1.762	1.00
10_F	57_L	1.748	1.00
92_L	96_E	1.722	1.00
138_Y	142_L	1.721	1.00
22_A	108_Q	1.712	1.00
32_K	36_E	1.708	1.00
213_D	221_M	1.693	1.00
148_Q	151_N	1.687	1.00
143_M	146_I	1.657	1.00
77_E	130_R	1.656	1.00
153_I	208_L	1.639	1.00
196_L	200_L	1.632	1.00
206_L	228_M	1.598	1.00
16_R	158_E	1.593	1.00
44_K	49_G	1.573	1.00
15_S	18_K	1.547	1.00
20_T	42_E	1.510	1.00
226_E	229_I	1.499	1.00
223_K	226_E	1.493	1.00
195_K	199_S	1.492	1.00
65_M	76_H	1.492	1.00
150_K	167_F	1.480	1.00
214_L	221_M	1.466	1.00
146_I	150_K	1.442	1.00
214_L	217_S	1.420	1.00
62_E	87_T	1.405	1.00
188_E	200_L	1.404	1.00
15_S	20_T	1.401	1.00
211_L	214_L	1.373	1.00
164_I	171_G	1.366	1.00
145_K	149_V	1.355	1.00
194_E	198_R	1.319	1.00
186_K	189_R	1.313	1.00
95_K	98_F	1.312	1.00
188_E	191_E	1.302	1.00
181_G	184_L	1.290	1.00
19_L	45_Y	1.281	1.00
149_V	152_E	1.277	1.00
41_R	52_E	1.243	1.00
224_E	228_M	1.236	1.00
11_A	111_A	1.225	1.00
20_T	40_V	1.217	1.00
222_V	226_E	1.215	1.00
72_F	88_L	1.200	1.00
139_R	226_E	1.199	1.00
206_L	209_K	1.193	1.00
43_F	49_G	1.183	1.00
52_E	55_K	1.178	1.00
196_L	199_S	1.177	1.00
11_A	79_L	1.161	1.00
193_K	196_L	1.159	1.00
57_L	63_G	1.148	1.00
188_E	193_K	1.147	1.00
40_V	108_Q	1.128	1.00
9_G	115_I	1.126	1.00
48_E	52_E	1.115	1.00
219_E	223_K	1.113	1.00
12_I	53_L	1.106	1.00
188_E	192_L	1.104	1.00
40_V	104_K	1.101	1.00
10_F	21_V	1.096	0.99
181_G	185_S	1.086	0.99
153_I	215_L	1.084	0.99
228_M	232_K	1.070	0.99
43_F	56_F	1.070	0.99
143_M	222_V	1.068	0.99
213_D	217_S	1.067	0.99
213_D	224_E	1.063	0.99
112_K	116_L	1.062	0.99
15_S	19_L	1.061	0.99
76_H	86_V	1.054	0.99
144_R	148_Q	1.051	0.99
50_I	75_L	1.047	0.99
168_D	171_G	1.047	0.99
209_K	224_E	1.043	0.99
76_H	88_L	1.037	0.99
170_R	173_A	1.035	0.99
165_E	171_G	1.022	0.99
49_G	52_E	1.020	0.99
54_I	82_K	1.012	0.99
64_I	89_I	1.010	0.99
108_Q	112_K	1.004	0.99
47_N	51_E	1.002	0.99
210_Q	224_E	0.997	0.99
210_Q	213_D	0.978	0.99
208_L	215_L	0.978	0.99
208_L	212_V	0.978	0.99
132_L	229_I	0.968	0.99
167_F	171_G	0.964	0.99
110_L	113_A	0.964	0.99
67_S	88_L	0.962	0.99
218_L	222_V	0.960	0.99
35_V	38_N	0.957	0.99
89_I	94_L	0.953	0.99
216_K	223_K	0.952	0.99
191_E	194_E	0.951	0.99
218_L	221_M	0.950	0.99
173_A	177_K	0.948	0.99
63_G	79_L	0.947	0.99
146_I	215_L	0.929	0.98
15_S	72_F	0.924	0.98
14_V	65_M	0.924	0.98
221_M	224_E	0.920	0.98
68_T	71_Y	0.913	0.98
62_E	85_K	0.907	0.98
141_S	152_E	0.906	0.98
97_V	113_A	0.898	0.98
166_P	171_G	0.891	0.98
193_K	197_G	0.889	0.98
206_L	210_Q	0.887	0.98
227_D	230_I	0.885	0.98
33_R	36_E	0.885	0.98
227_D	231_S	0.880	0.98
93_H	100_K	0.880	0.98
204_E	211_L	0.879	0.98
21_V	41_R	0.879	0.98
224_E	232_K	0.871	0.97
143_M	147_T	0.864	0.97
179_S	182_E	0.861	0.97
12_I	21_V	0.857	0.97
32_K	35_V	0.854	0.97
193_K	200_L	0.853	0.97
223_K	227_D	0.852	0.97
50_I	78_E	0.850	0.97
216_K	219_E	0.840	0.97
41_R	49_G	0.837	0.97
190_D	194_E	0.837	0.97
150_K	215_L	0.836	0.97
152_E	155_K	0.835	0.97
62_E	114_F	0.831	0.97
178_L	181_G	0.827	0.96
209_K	213_D	0.820	0.96
19_L	75_L	0.818	0.96
142_L	222_V	0.813	0.96
8_R	23_K	0.810	0.96
214_L	218_L	0.810	0.96
64_I	119_V	0.809	0.96
98_F	109_R	0.807	0.96
189_R	196_L	0.803	0.96
188_E	199_S	0.801	0.96
216_K	220_A	0.795	0.96
195_K	198_R	0.792	0.96
187_E	193_K	0.791	0.95
71_Y	90_N	0.791	0.95
43_F	50_I	0.782	0.95
50_I	74_Y	0.780	0.95
23_K	39_E	0.777	0.95
55_K	82_K	0.774	0.95
153_I	212_V	0.770	0.95
174_L	181_G	0.770	0.95
175_L	212_V	0.764	0.95
217_S	220_A	0.763	0.95
23_K	60_Y	0.763	0.95
64_I	94_L	0.763	0.95
190_D	193_K	0.752	0.94
193_K	199_S	0.751	0.94
226_E	230_I	0.750	0.94
194_E	197_G	0.749	0.94
140_E	144_R	0.746	0.94
169_K	173_A	0.743	0.94
52_E	56_F	0.739	0.94
51_E	55_K	0.736	0.93
201_N	212_V	0.734	0.93
14_V	19_L	0.733	0.93
146_I	222_V	0.731	0.93
178_L	184_L	0.729	0.93
126_T	129_I	0.728	0.93
145_K	214_L	0.727	0.93
70_P	158_E	0.724	0.93
142_L	146_I	0.719	0.93
32_K	38_N	0.717	0.92
179_S	183_G	0.715	0.92
138_Y	141_S	0.714	0.92
112_K	115_I	0.714	0.92
54_I	58_G	0.712	0.92
11_A	107_A	0.711	0.92
215_L	219_E	0.708	0.92
209_K	212_V	0.705	0.92
101_K	106_D	0.704	0.92
210_Q	228_M	0.702	0.92
187_E	191_E	0.700	0.92
126_T	130_R	0.699	0.92
130_R	134_E	0.697	0.91
172_I	176_E	0.696	0.91
95_K	99_G	0.695	0.91
173_A	195_K	0.694	0.91
141_S	144_R	0.693	0.91
67_S	91_P	0.685	0.91
58_G	84_Y	0.684	0.91
97_V	100_K	0.677	0.90
174_L	178_L	0.677	0.90
187_E	192_L	0.677	0.90
55_K	59_E	0.673	0.90
10_F	79_L	0.672	0.90
21_V	43_F	0.670	0.90
54_I	84_Y	0.669	0.90
43_F	53_L	0.669	0.90
75_L	79_L	0.668	0.90
19_L	43_F	0.666	0.89
94_L	105_L	0.664	0.89
149_V	153_I	0.663	0.89
172_I	179_S	0.663	0.89
174_L	179_S	0.660	0.89
12_I	18_K	0.654	0.89
80_T	124_I	0.652	0.88
197_G	203_A	0.648	0.88
78_E	81_E	0.648	0.88
89_I	119_V	0.645	0.88
20_T	104_K	0.644	0.88
9_G	114_F	0.639	0.87
173_A	194_E	0.638	0.87
83_G	231_S	0.633	0.87
49_G	53_L	0.632	0.87
83_G	220_A	0.628	0.87
195_K	200_L	0.623	0.86
80_T	121_G	0.622	0.86
212_V	219_E	0.620	0.86
67_S	102_T	0.616	0.85
187_E	200_L	0.612	0.85
110_L	115_I	0.611	0.85
178_L	182_E	0.610	0.85
22_A	40_V	0.606	0.85
138_Y	149_V	0.603	0.84
21_V	56_F	0.596	0.84
209_K	227_D	0.595	0.83
213_D	219_E	0.594	0.83
180_R	183_G	0.593	0.83
153_I	211_L	0.588	0.83
207_A	210_Q	0.588	0.83
118_A	153_I	0.587	0.83
189_R	199_S	0.586	0.83
14_V	71_Y	0.583	0.82
149_V	214_L	0.580	0.82
180_R	185_S	0.579	0.82
208_L	211_L	0.578	0.82
141_S	145_K	0.568	0.81
22_A	111_A	0.567	0.80
170_R	174_L	0.564	0.80
94_L	113_A	0.564	0.80
187_E	194_E	0.564	0.80
68_T	90_N	0.563	0.80
21_V	57_L	0.562	0.80
201_N	204_E	0.561	0.80
217_S	227_D	0.561	0.80
165_E	172_I	0.560	0.80
17_D	202_D	0.560	0.80
219_E	224_E	0.559	0.80
54_I	78_E	0.555	0.79
157_L	175_L	0.554	0.79
57_L	61_K	0.554	0.79
96_E	100_K	0.553	0.79
186_K	190_D	0.552	0.79
139_R	222_V	0.550	0.78
94_L	98_F	0.549	0.78
177_K	195_K	0.548	0.78
89_I	110_L	0.544	0.78
186_K	200_L	0.543	0.78
34_E	39_E	0.543	0.78
167_F	170_R	0.542	0.77
67_S	72_F	0.540	0.77
177_K	231_S	0.537	0.77
12_I	65_M	0.537	0.77
15_S	89_I	0.536	0.77
54_I	79_L	0.535	0.77
213_D	216_K	0.535	0.77
67_S	137_R	0.534	0.76
158_E	163_K	0.533	0.76
219_E	230_I	0.532	0.76
188_E	194_E	0.532	0.76
205_K	209_K	0.531	0.76
189_R	192_L	0.530	0.76
167_F	174_L	0.529	0.76
220_A	231_S	0.528	0.76
209_K	230_I	0.528	0.76
174_L	177_K	0.526	0.75
173_A	176_E	0.521	0.75
210_Q	225_V	0.521	0.75
141_S	147_T	0.519	0.74
113_A	119_V	0.519	0.74
202_D	228_M	0.519	0.74
224_E	227_D	0.516	0.74
22_A	37_V	0.514	0.74
165_E	168_D	0.514	0.74
217_S	228_M	0.513	0.74
141_S	148_Q	0.513	0.74
182_E	186_K	0.513	0.74
58_G	144_R	0.512	0.73
34_E	37_V	0.511	0.73
222_V	229_I	0.509	0.73
76_H	124_I	0.507	0.73
58_G	82_K	0.506	0.73
219_E	222_V	0.506	0.73
113_A	117_G	0.505	0.72
49_G	56_F	0.505	0.72
8_R	60_Y	0.504	0.72
190_D	198_R	0.502	0.72
177_K	181_G	0.502	0.72
211_L	228_M	0.502	0.72
45_Y	50_I	0.500	0.72

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1hjrA	2	0.4408	94.2	0.895	Contact Map
4ep4A	2	0.5469	91.6	0.904	Contact Map
1nu0A	1	0.4286	84.3	0.915	Contact Map
1vhxA	2	0.449	77.2	0.921	Contact Map
3cpeA	1	0.6735	67.7	0.927	Contact Map
3id6A	2	0.8571	66.2	0.928	Contact Map
3ifrA	2	0.6939	65.2	0.928	Contact Map
3c6aA	1	0.6367	64.5	0.928	Contact Map
1iv0A	1	0.3347	64.4	0.928	Contact Map
3plaA	2	0.8939	53.6	0.933	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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