Q97XK5 - SULSO - GREMLIN Contacts

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Q97XK5 - Transposase ISC1217

UniProt:	Q97XK5	Sequences: MTKELTREEYYKALEKAVNEVILSMTGTRKDVAKRLLLGAVVGRNATEIAQEAEMNYETVLNNLDKAAQVNLIEVVKKLVGDHPVLLIIDDTHDHKLYARAMPVSRNGAQIFYCRAHKRFESAIQLLVIGVKDLVNNQIYVIHIIAYIPRKVEEELKRRGEEVKFKTKIDALLEFLSSLSGLNVKSKVFDSWYVNSRTLQGNTVGELKSSARVVESGRSVPVSEFPQGEYLVEYLGTPIKLLVIDDYKGYGRRYFFSTDLNDTAEDIITTWENRWDIEVLIRELKALGLEGGSFLTWVRNSGFVALKALSLLVVQYFKYSTGLVLGAKRLARLIKSIYREAGGIKKLFKRRRKP	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	354 (299)
Sequences:	1057 (822)
Seq/√Len:	47.5

SULSO - Q97XK5 Q97XR1 Q97ZD8 Q97Y34 Q97YE4 Q97VQ0 Q7LX51 Q7LYK0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
189_F	205_G	3.824	1.00
129_I	179_L	3.429	1.00
145_I	178_S	3.358	1.00
36_L	303_F	3.057	1.00
166_K	174_E	3.051	1.00
89_I	172_L	3.008	1.00
287_L	307_K	2.836	1.00
221_P	227_Q	2.750	1.00
87_L	179_L	2.687	1.00
91_D	192_W	2.644	1.00
89_I	187_K	2.599	1.00
223_S	228_G	2.565	1.00
90_D	274_R	2.490	1.00
228_G	232_V	2.407	1.00
204_V	271_W	2.368	1.00
90_D	278_E	2.286	1.00
143_H	178_S	2.269	1.00
155_E	158_R	2.253	1.00
150_R	154_E	2.238	1.00
128_V	281_I	2.232	1.00
220_V	223_S	2.208	1.00
88_I	132_K	2.114	1.00
274_R	278_E	2.096	1.00
222_V	226_P	2.043	1.00
223_S	227_Q	2.038	1.00
142_I	182_L	2.018	0.99
224_E	228_G	2.013	0.99
127_L	193_Y	1.993	0.99
143_H	175_F	1.980	0.99
85_V	131_V	1.896	0.99
76_V	80_V	1.895	0.99
220_V	229_E	1.892	0.99
278_E	285_K	1.885	0.99
72_L	75_V	1.858	0.99
222_V	228_G	1.840	0.99
223_S	226_P	1.821	0.99
145_I	174_E	1.810	0.99
220_V	227_Q	1.796	0.99
293_S	299_R	1.761	0.99
91_D	168_K	1.743	0.98
91_D	193_Y	1.730	0.98
62_N	65_D	1.727	0.98
130_G	139_I	1.688	0.98
152_V	155_E	1.680	0.98
90_D	285_K	1.663	0.98
26_T	30_K	1.658	0.98
32_V	59_T	1.650	0.98
22_I	25_M	1.648	0.98
275_W	278_E	1.634	0.98
145_I	301_S	1.605	0.97
221_P	228_G	1.604	0.97
294_F	299_R	1.585	0.97
194_V	205_G	1.564	0.97
22_I	30_K	1.541	0.97
193_Y	274_R	1.539	0.97
75_V	80_V	1.531	0.96
242_L	267_I	1.513	0.96
63_N	292_G	1.504	0.96
227_Q	232_V	1.504	0.96
221_P	226_P	1.498	0.96
95_H	117_H	1.488	0.96
49_I	52_E	1.486	0.96
222_V	229_E	1.468	0.95
43_G	46_A	1.454	0.95
240_K	244_I	1.445	0.95
89_I	129_I	1.438	0.95
93_H	121_E	1.437	0.95
93_H	114_C	1.426	0.95
221_P	225_F	1.414	0.94
109_A	122_S	1.386	0.94
64_L	306_L	1.384	0.94
296_T	307_K	1.379	0.94
274_R	285_K	1.375	0.94
170_D	173_L	1.370	0.93
91_D	275_W	1.367	0.93
202_N	258_T	1.367	0.93
193_Y	285_K	1.364	0.93
152_V	158_R	1.353	0.93
86_L	270_T	1.344	0.93
223_S	230_Y	1.319	0.92
279_V	283_E	1.313	0.92
222_V	227_Q	1.309	0.92
194_V	200_Q	1.306	0.92
240_K	245_D	1.302	0.92
168_K	193_Y	1.295	0.91
33_A	37_L	1.282	0.91
206_E	253_R	1.279	0.91
199_L	205_G	1.266	0.90
220_V	228_G	1.262	0.90
69_Q	302_G	1.253	0.90
303_F	310_S	1.253	0.90
90_D	275_W	1.252	0.90
211_A	256_F	1.251	0.90
48_E	51_Q	1.245	0.90
94_D	102_M	1.241	0.89
228_G	231_L	1.229	0.89
130_G	281_I	1.225	0.89
25_M	29_R	1.224	0.89
21_V	24_S	1.220	0.89
19_N	23_L	1.211	0.88
75_V	79_L	1.210	0.88
92_T	278_E	1.209	0.88
225_F	228_G	1.205	0.88
72_L	76_V	1.205	0.88
114_C	121_E	1.202	0.88
297_W	301_S	1.194	0.88
108_G	288_G	1.193	0.88
230_Y	233_E	1.192	0.88
295_L	303_F	1.170	0.87
103_P	300_N	1.162	0.86
39_G	49_I	1.153	0.86
147_Y	165_F	1.149	0.86
275_W	285_K	1.147	0.85
243_V	246_D	1.147	0.85
186_S	270_T	1.146	0.85
223_S	232_V	1.142	0.85
190_D	278_E	1.137	0.85
220_V	232_V	1.132	0.85
102_M	124_I	1.130	0.85
154_E	157_K	1.127	0.84
276_D	311_L	1.127	0.84
96_K	109_A	1.121	0.84
224_E	227_Q	1.118	0.84
63_N	69_Q	1.111	0.84
260_L	263_T	1.108	0.83
129_I	175_F	1.096	0.83
63_N	67_A	1.089	0.82
240_K	243_V	1.088	0.82
203_T	258_T	1.084	0.82
292_G	300_N	1.082	0.82
40_A	45_N	1.081	0.82
86_L	188_V	1.081	0.82
167_T	170_D	1.071	0.81
188_V	270_T	1.070	0.81
304_V	307_K	1.067	0.81
93_H	112_F	1.063	0.81
29_R	67_A	1.062	0.81
145_I	171_A	1.060	0.81
103_P	297_W	1.057	0.80
108_G	148_I	1.052	0.80
115_R	192_W	1.050	0.80
221_P	233_E	1.047	0.80
244_I	247_Y	1.046	0.80
299_R	303_F	1.041	0.79
114_C	125_Q	1.035	0.79
88_I	188_V	1.033	0.79
221_P	224_E	1.033	0.79
224_E	230_Y	1.032	0.79
166_K	177_S	1.032	0.79
211_A	254_Y	1.030	0.79
195_N	210_S	1.028	0.78
275_W	314_V	1.026	0.78
306_L	310_S	1.026	0.78
130_G	280_L	1.023	0.78
28_T	31_D	1.022	0.78
231_L	234_Y	1.020	0.78
154_E	162_E	1.018	0.78
153_E	157_K	1.018	0.78
28_T	33_A	1.017	0.78
274_R	281_I	1.010	0.77
285_K	314_V	1.010	0.77
240_K	257_S	1.009	0.77
22_I	29_R	1.008	0.77
57_Y	61_L	1.006	0.77
200_Q	205_G	1.005	0.77
239_I	243_V	0.999	0.76
220_V	226_P	0.998	0.76
20_E	25_M	0.986	0.75
94_D	126_L	0.983	0.75
277_I	285_K	0.979	0.75
153_E	156_L	0.964	0.74
71_N	76_V	0.960	0.73
90_D	277_I	0.956	0.73
171_A	175_F	0.951	0.73
125_Q	168_K	0.945	0.72
141_V	280_L	0.943	0.72
58_E	62_N	0.938	0.72
92_T	275_W	0.935	0.71
32_V	35_R	0.928	0.71
204_V	257_S	0.926	0.71
38_L	41_V	0.926	0.71
226_P	229_E	0.919	0.70
127_L	171_A	0.918	0.70
207_L	211_A	0.910	0.69
101_A	129_I	0.909	0.69
246_D	249_G	0.909	0.69
21_V	25_M	0.908	0.69
87_L	184_V	0.908	0.69
205_G	256_F	0.905	0.69
281_I	285_K	0.901	0.69
76_V	308_A	0.900	0.69
103_P	124_I	0.900	0.69
255_F	271_W	0.898	0.68
141_V	311_L	0.896	0.68
28_T	36_L	0.896	0.68
34_K	38_L	0.895	0.68
184_V	282_R	0.892	0.68
128_V	141_V	0.891	0.68
24_S	71_N	0.885	0.67
252_R	309_L	0.884	0.67
170_D	234_Y	0.884	0.67
225_F	232_V	0.882	0.67
33_A	36_L	0.880	0.67
92_T	274_R	0.878	0.67
126_L	300_N	0.876	0.66
90_D	193_Y	0.875	0.66
124_I	300_N	0.874	0.66
224_E	231_L	0.874	0.66
124_I	148_I	0.873	0.66
224_E	229_E	0.873	0.66
141_V	312_L	0.872	0.66
159_R	162_E	0.869	0.66
115_R	121_E	0.865	0.65
221_P	229_E	0.865	0.65
220_V	225_F	0.861	0.65
112_F	124_I	0.856	0.65
127_L	145_I	0.854	0.64
215_E	218_R	0.854	0.64
189_F	194_V	0.853	0.64
139_I	280_L	0.852	0.64
112_F	115_R	0.847	0.64
190_D	274_R	0.846	0.64
18_V	34_K	0.841	0.63
220_V	224_E	0.838	0.63
227_Q	233_E	0.837	0.63
122_S	149_P	0.833	0.62
71_N	77_K	0.831	0.62
249_G	257_S	0.830	0.62
281_I	284_L	0.827	0.62
127_L	172_L	0.825	0.62
94_D	304_V	0.825	0.62
114_C	275_W	0.825	0.62
38_L	46_A	0.821	0.61
37_L	309_L	0.821	0.61
110_Q	151_K	0.818	0.61
207_L	278_E	0.816	0.61
168_K	192_W	0.816	0.61
153_E	158_R	0.816	0.61
77_K	80_V	0.815	0.61
221_P	231_L	0.814	0.61
91_D	281_I	0.808	0.60
284_L	289_L	0.806	0.60
139_I	276_D	0.806	0.60
257_S	293_S	0.805	0.60
245_D	251_G	0.804	0.60
114_C	120_F	0.801	0.59
227_Q	230_Y	0.801	0.59
126_L	304_V	0.798	0.59
117_H	121_E	0.797	0.59
36_L	40_A	0.795	0.59
221_P	232_V	0.793	0.59
98_Y	295_L	0.789	0.58
193_Y	275_W	0.788	0.58
159_R	163_V	0.787	0.58
90_D	168_K	0.786	0.58
147_Y	168_K	0.781	0.58
207_L	256_F	0.781	0.58
189_F	192_W	0.777	0.57
91_D	277_I	0.774	0.57
191_S	208_K	0.773	0.57
92_T	285_K	0.770	0.56
230_Y	235_L	0.768	0.56
74_E	210_S	0.767	0.56
64_L	302_G	0.764	0.56
111_I	122_S	0.760	0.55
21_V	27_G	0.760	0.55
73_I	77_K	0.759	0.55
95_H	121_E	0.758	0.55
82_D	136_N	0.756	0.55
129_I	187_K	0.751	0.55
157_K	162_E	0.746	0.54
127_L	168_K	0.745	0.54
83_H	156_L	0.745	0.54
94_D	128_V	0.744	0.54
196_S	290_E	0.742	0.54
125_Q	149_P	0.740	0.53
48_E	52_E	0.740	0.53
43_G	142_I	0.740	0.53
279_V	282_R	0.738	0.53
40_A	306_L	0.738	0.53
103_P	301_S	0.736	0.53
93_H	120_F	0.732	0.53
192_W	275_W	0.729	0.52
18_V	21_V	0.729	0.52
48_E	293_S	0.727	0.52
94_D	303_F	0.726	0.52
98_Y	109_A	0.725	0.52
27_G	77_K	0.725	0.52
153_E	162_E	0.721	0.52
83_H	233_E	0.715	0.51
102_M	146_A	0.714	0.51
90_D	190_D	0.714	0.51
225_F	229_E	0.713	0.51
241_L	254_Y	0.713	0.51
223_S	231_L	0.710	0.50
45_N	311_L	0.709	0.50
45_N	50_A	0.708	0.50
32_V	63_N	0.708	0.50
18_V	37_L	0.707	0.50
277_I	287_L	0.707	0.50
29_R	32_V	0.704	0.50
127_L	147_Y	0.704	0.50
144_I	311_L	0.704	0.50
271_W	274_R	0.702	0.50
79_L	316_Y	0.701	0.50
44_R	291_G	0.697	0.49
100_R	176_L	0.697	0.49
278_E	281_I	0.693	0.49
132_K	273_N	0.691	0.49
259_D	262_D	0.690	0.48
71_N	75_V	0.689	0.48
192_W	208_K	0.686	0.48
114_C	117_H	0.686	0.48
29_R	68_A	0.686	0.48
158_R	163_V	0.686	0.48
40_A	109_A	0.685	0.48
22_I	26_T	0.685	0.48
193_Y	278_E	0.678	0.47
111_I	118_K	0.675	0.47
18_V	33_A	0.668	0.46
26_T	32_V	0.666	0.46
112_F	121_E	0.666	0.46
69_Q	305_A	0.665	0.46
29_R	33_A	0.664	0.46
227_Q	231_L	0.664	0.46
21_V	34_K	0.658	0.45
275_W	281_I	0.652	0.45
189_F	208_K	0.650	0.44
102_M	126_L	0.650	0.44
49_I	292_G	0.648	0.44
127_L	289_L	0.648	0.44
29_R	296_T	0.648	0.44
93_H	125_Q	0.647	0.44
123_A	245_D	0.645	0.44
102_M	292_G	0.644	0.44
77_K	182_L	0.643	0.44
288_G	291_G	0.641	0.44
120_F	308_A	0.639	0.43
20_E	24_S	0.639	0.43
25_M	30_K	0.635	0.43
127_L	274_R	0.634	0.43
72_L	80_V	0.631	0.43
208_K	212_R	0.631	0.43
39_G	60_V	0.630	0.43
150_R	187_K	0.629	0.42
255_F	268_I	0.628	0.42
20_E	178_S	0.627	0.42
257_S	267_I	0.623	0.42
278_E	314_V	0.622	0.42
26_T	29_R	0.620	0.42
239_I	248_K	0.617	0.41
282_R	285_K	0.614	0.41
83_H	180_S	0.612	0.41
95_H	114_C	0.611	0.41
129_I	176_L	0.610	0.41
82_D	263_T	0.605	0.40
302_G	305_A	0.605	0.40
66_K	248_K	0.605	0.40
97_L	121_E	0.604	0.40
71_N	74_E	0.601	0.40
65_D	299_R	0.601	0.40
102_M	122_S	0.600	0.40
177_S	213_V	0.596	0.39
23_L	30_K	0.596	0.39
156_L	159_R	0.594	0.39
156_L	163_V	0.592	0.39
112_F	149_P	0.592	0.39
32_V	62_N	0.590	0.39
45_N	63_N	0.590	0.39
161_E	174_E	0.589	0.39
168_K	171_A	0.589	0.39
289_L	304_V	0.587	0.38
263_T	266_D	0.587	0.38
113_Y	183_N	0.587	0.38
68_A	302_G	0.583	0.38
298_V	302_G	0.582	0.38
178_S	273_N	0.580	0.38
300_N	304_V	0.580	0.38
102_M	300_N	0.580	0.38
111_I	253_R	0.577	0.37
31_D	68_A	0.576	0.37
301_S	305_A	0.573	0.37
21_V	213_V	0.573	0.37
69_Q	306_L	0.571	0.37
116_A	290_E	0.570	0.37
92_T	289_L	0.570	0.37
60_V	63_N	0.568	0.37
44_R	50_A	0.565	0.36
58_E	178_S	0.561	0.36
70_V	310_S	0.561	0.36
44_R	98_Y	0.559	0.36
60_V	142_I	0.558	0.36
191_S	207_L	0.558	0.36
210_S	250_Y	0.557	0.36
111_I	121_E	0.557	0.36
82_D	269_T	0.556	0.36
111_I	120_F	0.552	0.35
46_A	147_Y	0.551	0.35
46_A	293_S	0.551	0.35
161_E	164_K	0.548	0.35
295_L	310_S	0.548	0.35
86_L	272_E	0.548	0.35
58_E	292_G	0.545	0.35
114_C	290_E	0.545	0.35
57_Y	275_W	0.543	0.34
114_C	123_A	0.542	0.34
168_K	194_V	0.541	0.34
74_E	265_E	0.539	0.34
101_A	246_D	0.539	0.34
115_R	120_F	0.539	0.34
36_L	293_S	0.536	0.34
71_N	322_G	0.534	0.34
123_A	271_W	0.531	0.33
299_R	306_L	0.531	0.33
27_G	31_D	0.531	0.33
296_T	311_L	0.530	0.33
190_D	285_K	0.528	0.33
21_V	262_D	0.528	0.33
62_N	67_A	0.528	0.33
123_A	203_T	0.526	0.33
98_Y	108_G	0.526	0.33
102_M	293_S	0.526	0.33
239_I	242_L	0.525	0.33
85_V	185_K	0.524	0.33
75_V	78_K	0.523	0.33
50_A	312_L	0.522	0.32
292_G	299_R	0.522	0.32
76_V	79_L	0.522	0.32
90_D	130_G	0.521	0.32
38_L	157_K	0.521	0.32
40_A	284_L	0.521	0.32
172_L	209_S	0.521	0.32
87_L	255_F	0.520	0.32
142_I	160_G	0.517	0.32
156_L	162_E	0.516	0.32
67_A	148_I	0.515	0.32
92_T	304_V	0.513	0.32
91_D	191_S	0.512	0.32
54_E	67_A	0.509	0.31
172_L	198_T	0.508	0.31
188_V	273_N	0.508	0.31
154_E	164_K	0.507	0.31
146_A	297_W	0.506	0.31
283_E	286_A	0.505	0.31
67_A	302_G	0.505	0.31
197_R	251_G	0.503	0.31
36_L	299_R	0.501	0.31
148_I	152_V	0.500	0.31

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1musA	2	0.9322	99.9	0.719	Contact Map
2kkoA	2	0.3023	15.1	0.967	Contact Map
3hdeA	2	0.2825	15.1	0.967	Contact Map
4pc4A	1	0.5734	13.9	0.967	Contact Map
3hdfA	1	0.2797	11	0.969	Contact Map
2j49A	1	0.2288	10.9	0.969	Contact Map
3qfiA	1	0.2571	10	0.969	Contact Map
1xjuA	2	0.2881	9.5	0.97	Contact Map
1xjtA	1	0.2881	8.8	0.97	Contact Map
2zkzA	2	0.2514	8.6	0.97	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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