Q97XB6 - SULSO - GREMLIN Contacts

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Q97XB6 - Second ORF in transposon ISC1904

UniProt:	Q97XB6	Sequences: MLKNLRIRKFEPEEEYVYFTYSIKNSEREKSKELIKEYRTLLQKAIDHLWNLTKIQVRKNGNYKITLPKKKEVYKPLREELEKINHLASHYVDKAINDAFSILTSWRKRAIKGRASIEKPRVKKAYVRIKTTLRKVVGESVRITVRPYEYITFSWSKSWFSRRVRELELGEPIIKEEKVYLPFRYKLPWVTPLNFLAIDSNLYTLDAYDGEKFVTISLKQLYSLKYSMEVKRAKVQSFASKHTKRGRELMRKYSHRERNRVLDFVHKFVNTLLDLYPMTFFAVEKLNKESMFKDANDSLSRKISRTVWRSIHRVLKYKAPLYGSFVKEVNPYLTSRSCPRCRFVSRKVGKTFECERCGFKLDRQLNASLNIYLKMCGFPHIRSRVWVGVIPLMGRRGMNVRDFGEAQGLRVDIKYHEIL	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	419 (345)
Sequences:	4412 (2822)
Seq/√Len:	151.9

ECOLI - INSQ
HALSA - Q9HSW9 Q9HHJ9 Q9HM87 Q9HSW7 Q9HHG8 Q9HSZ6 Q9HSD4 Q9HHU6 Q9HSY4
SULSO - Q97XB6 Q97YI5 Q97Z61 Q97XQ0 Q97U51 Q97U68 Q97UQ8 Q97Y48 Q97YW9 Q97YU1 Q97XQ8 Q97V47 Q97X39 Q97YI2 Q97Y44 Q97V25 Q97XP1 Q97YW4 Q97WZ3 Q97Y67 Q97XA4 Q97V53 Q7LXH2 Q97WY6 Q7LXD9 Q7LXH6 Q97YF7 Q97U09 Y2105 Q7LX59

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
263_D	267_K	5.283	1.00
287_N	290_S	4.374	1.00
339_P	366_N	4.226	1.00
319_A	326_V	3.617	1.00
282_A	375_M	3.054	1.00
205_L	311_I	2.988	1.00
312_H	328_E	2.936	1.00
283_V	315_L	2.885	1.00
19_F	321_L	2.877	1.00
310_S	314_V	2.766	1.00
270_N	274_D	2.701	1.00
310_S	313_R	2.400	1.00
333_L	336_R	2.374	1.00
172_P	181_L	2.343	1.00
316_K	328_E	2.323	1.00
311_I	315_L	2.317	1.00
329_V	371_I	2.227	1.00
273_L	319_A	2.219	1.00
225_K	301_R	2.211	1.00
292_F	304_S	2.155	1.00
170_G	186_K	2.152	1.00
281_F	319_A	2.125	1.00
266_H	317_Y	2.109	1.00
54_K	119_K	2.101	1.00
266_H	314_V	2.078	1.00
334_T	371_I	2.064	1.00
283_V	326_V	2.057	1.00
224_L	260_R	2.037	1.00
231_K	246_G	2.006	1.00
353_E	360_K	2.003	1.00
338_C	341_C	2.001	1.00
341_C	357_C	1.977	1.00
374_K	377_G	1.957	1.00
51_N	55_I	1.950	1.00
28_R	31_S	1.935	1.00
345_S	348_V	1.932	1.00
277_P	324_S	1.908	1.00
36_K	179_V	1.905	1.00
350_K	363_R	1.904	1.00
297_D	300_S	1.892	1.00
316_K	320_P	1.885	1.00
345_S	349_G	1.879	1.00
269_V	314_V	1.879	1.00
205_L	264_F	1.876	1.00
49_L	96_I	1.872	1.00
337_S	344_V	1.848	1.00
262_L	310_S	1.834	1.00
341_C	354_C	1.827	1.00
354_C	357_C	1.770	1.00
37_E	86_H	1.769	1.00
265_V	314_V	1.758	1.00
352_F	361_L	1.754	1.00
246_G	250_M	1.738	1.00
266_H	318_K	1.732	1.00
67_L	71_K	1.725	1.00
338_C	357_C	1.723	1.00
50_W	99_A	1.702	1.00
241_K	244_K	1.686	1.00
336_R	346_R	1.683	1.00
266_H	269_V	1.670	1.00
283_V	328_E	1.652	1.00
338_C	354_C	1.647	1.00
340_R	359_F	1.646	1.00
244_K	247_R	1.618	1.00
265_V	310_S	1.600	1.00
35_I	179_V	1.593	1.00
231_K	234_K	1.581	1.00
44_K	83_K	1.561	1.00
198_I	206_D	1.530	1.00
65_I	70_K	1.528	1.00
143_I	181_L	1.527	1.00
195_F	372_Y	1.527	1.00
20_T	182_P	1.513	1.00
33_E	37_E	1.509	1.00
285_K	328_E	1.504	1.00
283_V	312_H	1.501	1.00
346_R	349_G	1.499	1.00
351_T	360_K	1.482	1.00
68_P	71_K	1.467	1.00
176_E	255_H	1.454	1.00
352_F	359_F	1.446	1.00
207_A	365_L	1.434	1.00
343_F	355_E	1.426	1.00
284_E	331_P	1.401	1.00
49_L	99_A	1.386	1.00
109_R	117_I	1.382	1.00
110_A	117_I	1.382	1.00
144_T	150_Y	1.379	1.00
65_I	69_K	1.379	1.00
32_K	36_K	1.373	1.00
39_R	179_V	1.371	1.00
269_V	317_Y	1.365	1.00
235_V	247_R	1.353	1.00
100_F	104_T	1.349	1.00
105_S	117_I	1.349	1.00
316_K	326_V	1.342	1.00
238_F	246_G	1.341	1.00
204_T	207_A	1.341	1.00
369_L	373_L	1.338	1.00
178_K	255_H	1.335	1.00
208_Y	212_K	1.323	1.00
349_G	363_R	1.316	1.00
269_V	318_K	1.315	1.00
255_H	259_N	1.304	1.00
294_D	300_S	1.302	1.00
104_T	108_K	1.299	1.00
303_I	308_W	1.283	1.00
90_H	130_K	1.279	1.00
196_L	208_Y	1.263	1.00
68_P	72_E	1.244	1.00
66_T	69_K	1.233	1.00
24_K	178_K	1.208	1.00
228_M	250_M	1.208	1.00
90_H	132_T	1.200	1.00
228_M	254_S	1.200	1.00
195_F	280_F	1.196	1.00
269_V	311_I	1.195	1.00
231_K	253_Y	1.194	1.00
103_L	119_K	1.190	1.00
42_L	95_A	1.187	1.00
75_K	78_R	1.184	1.00
98_D	128_R	1.173	1.00
232_R	236_Q	1.165	1.00
339_P	369_L	1.164	1.00
101_S	105_S	1.160	1.00
271_T	275_L	1.155	1.00
224_L	257_E	1.154	1.00
339_P	361_L	1.150	1.00
54_K	58_R	1.148	1.00
78_R	92_V	1.144	1.00
273_L	322_Y	1.142	1.00
314_V	317_Y	1.139	1.00
196_L	276_Y	1.135	1.00
94_K	97_N	1.124	1.00
109_R	118_E	1.123	1.00
29_E	32_K	1.121	0.99
269_V	315_L	1.120	0.99
265_V	311_I	1.117	0.99
200_S	308_W	1.116	0.99
27_E	31_S	1.115	0.99
287_N	331_P	1.115	0.99
350_K	362_D	1.103	0.99
337_S	374_K	1.101	0.99
198_I	208_Y	1.100	0.99
262_L	314_V	1.095	0.99
365_L	369_L	1.093	0.99
197_A	372_Y	1.090	0.99
258_R	262_L	1.087	0.99
127_V	131_T	1.075	0.99
202_L	306_T	1.068	0.99
361_L	369_L	1.067	0.99
318_K	321_L	1.065	0.99
270_N	322_Y	1.058	0.99
239_A	243_T	1.057	0.99
57_V	61_G	1.051	0.99
69_K	72_E	1.043	0.99
192_P	277_P	1.042	0.99
200_S	312_H	1.038	0.99
234_K	238_F	1.038	0.99
273_L	281_F	1.037	0.99
212_K	271_T	1.037	0.99
227_S	231_K	1.033	0.99
265_V	269_V	1.033	0.99
274_D	322_Y	1.026	0.99
21_Y	185_Y	1.024	0.99
329_V	374_K	1.024	0.99
195_F	376_C	1.023	0.99
273_L	324_S	1.022	0.99
334_T	374_K	1.016	0.99
313_R	316_K	1.013	0.99
55_I	59_K	1.013	0.99
145_V	174_I	1.013	0.99
105_S	109_R	1.007	0.99
345_S	352_F	1.004	0.99
46_I	95_A	1.003	0.99
339_P	359_F	1.001	0.99
204_T	215_T	1.001	0.99
348_V	363_R	0.998	0.99
329_V	375_M	0.997	0.99
231_K	249_L	0.993	0.99
127_V	130_K	0.992	0.99
207_A	213_F	0.992	0.99
88_A	91_Y	0.990	0.99
231_K	250_M	0.986	0.99
105_S	110_A	0.982	0.99
285_K	331_P	0.979	0.99
294_D	298_S	0.978	0.99
288_K	309_R	0.972	0.99
76_P	80_E	0.970	0.99
235_V	250_M	0.968	0.99
32_K	179_V	0.963	0.98
54_K	103_L	0.962	0.98
336_R	348_V	0.961	0.98
213_F	362_D	0.961	0.98
361_L	365_L	0.960	0.98
343_F	352_F	0.959	0.98
106_W	120_P	0.953	0.98
70_K	73_V	0.951	0.98
243_T	247_R	0.951	0.98
66_T	70_K	0.949	0.98
196_L	209_D	0.948	0.98
232_R	250_M	0.948	0.98
52_L	73_V	0.946	0.98
135_K	144_T	0.944	0.98
72_E	75_K	0.941	0.98
67_L	97_N	0.941	0.98
27_E	35_I	0.932	0.98
40_T	43_Q	0.931	0.98
46_I	102_I	0.929	0.98
50_W	103_L	0.927	0.98
133_L	146_R	0.927	0.98
352_F	363_R	0.926	0.98
282_A	371_I	0.920	0.98
65_I	74_Y	0.917	0.98
106_W	119_K	0.916	0.98
280_F	325_F	0.914	0.98
142_R	150_Y	0.913	0.98
155_W	158_S	0.910	0.98
90_H	94_K	0.905	0.98
249_L	252_K	0.903	0.98
46_I	99_A	0.898	0.98
56_Q	60_N	0.896	0.98
135_K	138_G	0.892	0.97
229_E	233_A	0.889	0.97
294_D	297_D	0.888	0.97
44_K	48_H	0.887	0.97
74_Y	78_R	0.886	0.97
18_Y	184_R	0.885	0.97
217_S	306_T	0.885	0.97
90_H	131_T	0.882	0.97
105_S	108_K	0.878	0.97
351_T	361_L	0.877	0.97
94_K	128_R	0.868	0.97
333_L	374_K	0.867	0.97
365_L	368_S	0.861	0.97
133_L	136_V	0.860	0.97
195_F	282_A	0.860	0.97
272_L	281_F	0.859	0.97
343_F	356_R	0.857	0.97
288_K	308_W	0.856	0.97
84_I	92_V	0.854	0.97
339_P	367_A	0.849	0.97
345_S	355_E	0.846	0.97
265_V	307_V	0.842	0.96
367_A	370_N	0.842	0.96
202_L	302_K	0.837	0.96
256_R	260_R	0.836	0.96
366_N	370_N	0.836	0.96
65_I	68_P	0.835	0.96
41_L	83_K	0.835	0.96
331_P	334_T	0.834	0.96
206_D	268_F	0.831	0.96
319_A	324_S	0.830	0.96
340_R	369_L	0.829	0.96
200_S	206_D	0.828	0.96
128_R	171_E	0.823	0.96
98_D	173_I	0.820	0.96
143_I	172_P	0.819	0.96
292_F	300_S	0.817	0.96
280_F	327_K	0.817	0.96
253_Y	257_E	0.816	0.96
93_D	97_N	0.814	0.96
240_S	243_T	0.813	0.96
48_H	52_L	0.811	0.96
220_Q	260_R	0.807	0.95
53_T	100_F	0.807	0.95
290_S	303_I	0.806	0.95
71_K	96_I	0.804	0.95
285_K	312_H	0.803	0.95
369_L	372_Y	0.802	0.95
220_Q	224_L	0.798	0.95
290_S	331_P	0.797	0.95
232_R	254_S	0.796	0.95
18_Y	186_K	0.796	0.95
228_M	257_E	0.788	0.95
57_V	103_L	0.786	0.95
247_R	251_R	0.786	0.95
71_K	75_K	0.782	0.95
53_T	103_L	0.781	0.95
229_E	232_R	0.780	0.95
52_L	56_Q	0.780	0.95
299_L	303_I	0.778	0.94
137_V	140_S	0.776	0.94
94_K	98_D	0.773	0.94
270_N	318_K	0.771	0.94
204_T	213_F	0.769	0.94
173_I	182_P	0.766	0.94
153_F	156_S	0.765	0.94
23_I	181_L	0.764	0.94
203_Y	207_A	0.761	0.94
70_K	74_Y	0.761	0.94
272_L	276_Y	0.757	0.94
257_E	305_R	0.756	0.94
281_F	315_L	0.747	0.93
228_M	236_Q	0.747	0.93
96_I	100_F	0.747	0.93
196_L	281_F	0.744	0.93
199_D	364_Q	0.743	0.93
235_V	246_G	0.738	0.93
102_I	120_P	0.738	0.93
374_K	378_F	0.736	0.93
35_I	39_R	0.735	0.93
25_N	181_L	0.735	0.93
333_L	337_S	0.733	0.93
174_I	181_L	0.732	0.92
41_L	87_L	0.730	0.92
194_N	210_G	0.729	0.92
346_R	352_F	0.726	0.92
228_M	232_R	0.726	0.92
229_E	236_Q	0.726	0.92
48_H	77_L	0.724	0.92
309_R	313_R	0.724	0.92
129_I	145_V	0.723	0.92
268_F	271_T	0.722	0.92
38_Y	42_L	0.720	0.92
264_F	268_F	0.719	0.92
286_L	331_P	0.717	0.92
148_Y	151_I	0.716	0.92
34_L	38_Y	0.715	0.92
94_K	130_K	0.714	0.92
336_R	344_V	0.713	0.91
87_L	90_H	0.710	0.91
224_L	256_R	0.710	0.91
45_A	95_A	0.710	0.91
337_S	370_N	0.708	0.91
24_K	180_Y	0.708	0.91
29_E	33_E	0.706	0.91
339_P	373_L	0.706	0.91
191_T	194_N	0.703	0.91
282_A	372_Y	0.703	0.91
217_S	220_Q	0.703	0.91
103_L	107_R	0.699	0.91
58_R	61_G	0.699	0.91
67_L	72_E	0.698	0.91
99_A	103_L	0.695	0.90
67_L	70_K	0.693	0.90
214_V	264_F	0.692	0.90
40_T	44_K	0.691	0.90
282_A	327_K	0.690	0.90
41_L	44_K	0.690	0.90
171_E	184_R	0.690	0.90
168_E	186_K	0.686	0.90
201_N	207_A	0.686	0.90
327_K	375_M	0.685	0.90
134_R	169_L	0.684	0.90
53_T	70_K	0.684	0.90
205_L	306_T	0.683	0.90
65_I	73_V	0.682	0.90
90_H	93_D	0.682	0.90
315_L	326_V	0.681	0.90
128_R	173_I	0.679	0.89
213_F	365_L	0.673	0.89
132_T	135_K	0.671	0.89
37_E	41_L	0.668	0.89
189_W	324_S	0.668	0.89
203_Y	350_K	0.668	0.89
235_V	243_T	0.666	0.88
75_K	79_E	0.661	0.88
206_D	311_I	0.661	0.88
274_D	280_F	0.661	0.88
126_Y	130_K	0.659	0.88
205_L	269_V	0.656	0.88
234_K	246_G	0.656	0.88
345_S	363_R	0.654	0.88
103_L	106_W	0.654	0.88
69_K	74_Y	0.652	0.87
361_L	366_N	0.651	0.87
22_S	182_P	0.651	0.87
67_L	96_I	0.651	0.87
23_I	157_K	0.645	0.87
137_V	144_T	0.645	0.87
344_V	347_K	0.645	0.87
142_R	145_V	0.639	0.86
294_D	303_I	0.639	0.86
52_L	55_I	0.639	0.86
130_K	133_L	0.638	0.86
236_Q	250_M	0.635	0.86
231_K	235_V	0.634	0.86
145_V	148_Y	0.633	0.86
90_H	133_L	0.633	0.86
74_Y	77_L	0.632	0.86
233_A	237_S	0.632	0.86
189_W	192_P	0.632	0.86
178_K	259_N	0.630	0.86
157_K	160_F	0.630	0.86
355_E	363_R	0.629	0.85
221_L	224_L	0.629	0.85
38_Y	88_A	0.628	0.85
89_S	130_K	0.628	0.85
17_V	186_K	0.627	0.85
224_L	228_M	0.626	0.85
95_A	99_A	0.624	0.85
128_R	131_T	0.624	0.85
30_K	33_E	0.622	0.85
74_Y	93_D	0.621	0.85
96_I	99_A	0.621	0.85
73_V	76_P	0.620	0.85
175_K	178_K	0.617	0.84
276_Y	281_F	0.616	0.84
20_T	184_R	0.616	0.84
202_L	293_K	0.616	0.84
51_N	122_V	0.613	0.84
75_K	96_I	0.612	0.84
261_V	305_R	0.612	0.84
340_R	361_L	0.612	0.84
319_A	322_Y	0.610	0.84
44_K	81_L	0.609	0.84
224_L	253_Y	0.609	0.84
27_E	32_K	0.606	0.83
262_L	266_H	0.603	0.83
60_N	63_Y	0.601	0.83
18_Y	170_G	0.601	0.83
153_F	157_K	0.599	0.83
34_L	37_E	0.599	0.83
233_A	236_Q	0.598	0.83
371_I	374_K	0.597	0.82
106_W	109_R	0.596	0.82
145_V	151_I	0.596	0.82
230_V	234_K	0.596	0.82
234_K	237_S	0.595	0.82
51_N	54_K	0.595	0.82
26_S	154_S	0.594	0.82
288_K	303_I	0.592	0.82
314_V	318_K	0.589	0.82
208_Y	214_V	0.588	0.81
194_N	276_Y	0.588	0.81
134_R	138_G	0.587	0.81
317_Y	321_L	0.587	0.81
38_Y	129_I	0.587	0.81
69_K	73_V	0.586	0.81
234_K	249_L	0.584	0.81
243_T	250_M	0.583	0.81
297_D	304_S	0.583	0.81
244_K	248_E	0.583	0.81
89_S	132_T	0.583	0.81
333_L	352_F	0.581	0.81
38_Y	87_L	0.579	0.80
126_Y	129_I	0.577	0.80
288_K	291_M	0.572	0.80
254_S	261_V	0.571	0.80
52_L	77_L	0.570	0.79
92_V	95_A	0.569	0.79
91_Y	127_V	0.569	0.79
330_N	333_L	0.567	0.79
70_K	93_D	0.566	0.79
89_S	133_L	0.565	0.79
39_R	127_V	0.565	0.79
57_V	106_W	0.564	0.79
222_Y	225_K	0.564	0.79
156_S	159_W	0.563	0.79
312_H	316_K	0.562	0.78
200_S	283_V	0.562	0.78
75_K	80_E	0.561	0.78
108_K	117_I	0.560	0.78
282_A	374_K	0.560	0.78
22_S	262_L	0.559	0.78
337_S	352_F	0.558	0.78
227_S	253_Y	0.554	0.78
207_A	215_T	0.553	0.77
127_V	133_L	0.552	0.77
243_T	246_G	0.551	0.77
49_L	77_L	0.550	0.77
199_D	335_S	0.549	0.77
48_H	83_K	0.548	0.77
268_F	311_I	0.548	0.77
87_L	92_V	0.548	0.77
332_Y	336_R	0.546	0.76
238_F	242_H	0.546	0.76
254_S	258_R	0.545	0.76
209_D	365_L	0.544	0.76
265_V	268_F	0.543	0.76
235_V	238_F	0.542	0.76
242_H	245_R	0.542	0.76
299_L	302_K	0.541	0.76
240_S	244_K	0.541	0.76
205_L	265_V	0.539	0.76
95_A	98_D	0.538	0.75
27_E	151_I	0.535	0.75
373_L	378_F	0.533	0.75
56_Q	61_G	0.532	0.75
289_E	309_R	0.532	0.75
222_Y	302_K	0.530	0.74
229_E	297_D	0.530	0.74
53_T	56_Q	0.530	0.74
267_K	270_N	0.528	0.74
280_F	375_M	0.526	0.74
25_N	153_F	0.525	0.74
160_F	166_E	0.525	0.74
122_V	125_A	0.522	0.73
42_L	127_V	0.522	0.73
21_Y	157_K	0.520	0.73
257_E	261_V	0.519	0.73
134_R	140_S	0.519	0.73
66_T	72_E	0.518	0.73
68_P	74_Y	0.517	0.73
130_K	134_R	0.516	0.72
57_V	107_R	0.516	0.72
74_Y	96_I	0.514	0.72
222_Y	301_R	0.513	0.72
107_R	110_A	0.512	0.72
46_I	50_W	0.511	0.72
259_N	263_D	0.510	0.72
353_E	361_L	0.510	0.72
180_Y	255_H	0.509	0.71
41_L	92_V	0.509	0.71
371_I	376_C	0.508	0.71
229_E	301_R	0.508	0.71
290_S	294_D	0.507	0.71
155_W	159_W	0.507	0.71
270_N	314_V	0.506	0.71
362_D	367_A	0.505	0.71
202_L	292_F	0.504	0.71
30_K	34_L	0.503	0.71
37_E	87_L	0.503	0.71
41_L	84_I	0.502	0.70
88_A	146_R	0.501	0.70
348_V	362_D	0.500	0.70
214_V	271_T	0.500	0.70

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1nu0A	1	0.3103	93.4	0.938	Contact Map
1vhxA	2	0.327	91.7	0.941	Contact Map
1iv0A	1	0.2315	88.8	0.945	Contact Map
3j20Y	1	0.1146	86.7	0.947	Contact Map
4aybP	1	0.0955	86.6	0.947	Contact Map
2k4xA	1	0.1265	85.9	0.947	Contact Map
1qypA	1	0.1026	85.1	0.948	Contact Map
3j3w0	1	0.1265	76	0.952	Contact Map
1ffkW	1	0.1671	75.8	0.953	Contact Map
1vd4A	1	0.1265	69.8	0.955	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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