Q97XA4 - SULSO - GREMLIN Contacts

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Q97XA4 - Transposase ISC1316

UniProt:	Q97XA4	Sequences: MKLACKIYNTLRWADIYFYQRDGKGLTQTELRQLALDLRKQDDEYKQLYSQVVQQIADRYYEARQRFFEGLARFPKEKKPHKYYSLVYPQYGWKILQVREIRKGSKKNKKRLITLKLSNLGTFKVIIHRDFPLDKVKRVVVKLTRSERIYITFVVEDYEFPKLPNTGKVVAIDVGIEKLIVTSDGEYFPNLRPYEKALWKVKHLHRELSRKKFLSNNWFKAKVKLARAYEHLKNLRTDLYMKLGKWFAEHYDVVVMEDIHVKQLIGKSLRSLRRRLSDVAFSELRDLIKYQLEKYGKKLILVNPAYTSKTCARCGYVKEDLSLSDRVFVCSNCGWIADRDYNASLNILRGSGSERPLVWSSALYQYQHFGTGMAEL	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	376 (331)
Sequences:	4592 (2896)
Seq/√Len:	159.2

ECOLI - INSQ
HALSA - Q9HSW9 Q9HHJ9 Q9HSD4 Q9HM87 Q9HSZ6 Q9HHG8 Q9HSW7 Q9HHU6 Q9HSY4 Q9HHX8
SULSO - Q97XA4 Q97V53 Q97XP1 Q97V25 Q97V47 Q97XQ8 Q97Y44 Q97YW4 Q97YI2 Q97YI5 Q97YU1 Q97U68 Q97XB6 Q97Y48 Q97XQ0 Q97Z61 Q97UQ8 Q97U51 Q97Y67 Q97YW9 Q7LXD9 Q97WY6 Q97WZ3 Q97X39 Q7LXH2 Q7LXH6 Q97YF7 Q97U09 Q7LX59 Q97YK6 Q7LX75 Y2105 Q7LXH9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
238_D	242_K	5.253	1.00
260_H	263_Q	4.252	1.00
312_A	342_N	4.198	1.00
292_L	299_L	3.520	1.00
179_L	284_L	2.996	1.00
285_R	301_L	2.960	1.00
255_V	351_S	2.949	1.00
256_M	288_I	2.911	1.00
245_K	249_E	2.897	1.00
283_E	287_L	2.761	1.00
322_S	325_D	2.710	1.00
139_V	151_I	2.588	1.00
265_I	277_S	2.466	1.00
198_L	274_R	2.436	1.00
283_E	286_D	2.359	1.00
248_A	292_L	2.339	1.00
306_Y	309_K	2.325	1.00
310_T	317_V	2.317	1.00
350_G	353_S	2.299	1.00
289_K	301_L	2.297	1.00
302_V	347_I	2.224	1.00
204_L	221_A	2.154	1.00
284_L	288_I	2.139	1.00
254_V	292_L	2.138	1.00
137_K	156_E	2.131	1.00
329_V	336_I	2.102	1.00
307_T	347_I	2.039	1.00
241_M	290_Y	2.036	1.00
241_M	287_L	2.033	1.00
311_C	314_C	2.003	1.00
11_L	57_A	1.997	1.00
197_A	235_L	1.994	1.00
13_W	17_Y	1.986	1.00
28_Q	32_R	1.986	1.00
314_C	333_C	1.961	1.00
309_K	321_L	1.956	1.00
256_M	299_L	1.952	1.00
237_T	283_E	1.947	1.00
270_R	273_R	1.933	1.00
314_C	330_C	1.912	1.00
29_T	32_R	1.901	1.00
244_G	287_L	1.896	1.00
28_Q	58_D	1.880	1.00
221_A	225_L	1.854	1.00
16_I	74_F	1.843	1.00
252_D	297_K	1.813	1.00
241_M	291_Q	1.804	1.00
289_K	293_E	1.798	1.00
326_R	339_R	1.787	1.00
330_C	333_C	1.774	1.00
313_R	335_W	1.767	1.00
216_N	219_F	1.761	1.00
179_L	239_L	1.751	1.00
241_M	244_G	1.749	1.00
311_C	330_C	1.715	1.00
240_Y	287_L	1.709	1.00
311_C	333_C	1.687	1.00
240_Y	283_E	1.651	1.00
6_K	44_E	1.630	1.00
328_F	337_A	1.628	1.00
208_L	222_K	1.615	1.00
211_K	221_A	1.613	1.00
12_R	60_Y	1.597	1.00
258_D	301_L	1.596	1.00
59_R	87_V	1.587	1.00
256_M	301_L	1.580	1.00
115_L	151_I	1.563	1.00
172_I	180_I	1.553	1.00
70_G	73_R	1.546	1.00
256_M	285_R	1.523	1.00
219_F	222_K	1.516	1.00
16_I	20_Q	1.501	1.00
328_F	335_W	1.497	1.00
51_Q	90_Q	1.481	1.00
244_G	290_Y	1.460	1.00
321_L	325_D	1.456	1.00
327_V	336_I	1.446	1.00
207_E	211_K	1.437	1.00
61_Y	65_Q	1.435	1.00
345_L	349_R	1.421	1.00
257_E	304_P	1.412	1.00
51_Q	89_P	1.406	1.00
181_V	341_Y	1.379	1.00
66_R	72_A	1.366	1.00
169_V	348_L	1.358	1.00
170_V	182_T	1.358	1.00
64_R	74_F	1.348	1.00
316_Y	331_S	1.344	1.00
244_G	291_Q	1.333	1.00
204_L	207_E	1.328	1.00
17_Y	21_R	1.321	1.00
244_G	284_L	1.304	1.00
276_L	281_F	1.294	1.00
214_L	218_W	1.290	1.00
323_L	326_R	1.289	1.00
15_D	31_L	1.278	1.00
170_V	251_Y	1.277	1.00
178_K	181_V	1.276	1.00
11_L	60_Y	1.265	1.00
29_T	33_Q	1.262	1.00
148_R	230_E	1.262	1.00
182_T	186_E	1.258	1.00
312_A	345_L	1.253	1.00
289_K	299_L	1.251	1.00
146_S	230_E	1.242	1.00
248_A	295_Y	1.241	1.00
318_K	328_F	1.240	1.00
27_T	30_E	1.235	1.00
200_K	204_L	1.234	1.00
230_E	234_N	1.229	1.00
187_Y	338_D	1.224	1.00
318_K	321_L	1.208	1.00
201_V	225_L	1.201	1.00
32_R	58_D	1.196	1.00
144_T	147_E	1.182	1.00
309_K	320_D	1.180	1.00
169_V	253_V	1.177	1.00
172_I	182_T	1.168	1.00
201_V	229_Y	1.161	1.00
197_A	232_L	1.155	1.00
55_Q	58_D	1.154	1.00
35_A	57_A	1.153	1.00
14_A	34_L	1.148	1.00
237_T	287_L	1.138	1.00
174_V	281_F	1.136	1.00
260_H	304_P	1.127	1.00
65_Q	69_E	1.127	1.00
246_W	250_H	1.126	1.00
181_V	187_Y	1.121	1.00
341_Y	345_L	1.121	1.00
309_K	319_E	1.120	1.00
312_A	337_A	1.115	1.00
62_E	66_R	1.107	1.00
208_L	221_A	1.101	1.00
240_Y	284_L	1.094	0.99
176_I	279_V	1.091	0.99
33_Q	36_L	1.091	0.99
337_A	345_L	1.075	0.99
183_S	344_S	1.075	0.99
32_R	57_A	1.072	0.99
208_L	225_L	1.070	0.99
204_L	228_A	1.067	0.99
144_T	148_R	1.066	0.99
245_K	295_Y	1.055	0.99
49_Y	52_V	1.055	0.99
142_K	152_T	1.052	0.99
28_Q	57_A	1.048	0.99
4_A	56_I	1.047	0.99
323_L	339_R	1.045	0.99
287_L	290_Y	1.039	0.99
39_R	53_V	1.030	0.99
265_I	273_R	1.029	0.99
244_G	288_I	1.028	0.99
322_S	339_R	1.027	0.99
94_K	116_K	1.022	0.99
318_K	331_S	1.021	0.99
174_V	285_R	1.020	0.99
94_K	97_Q	1.018	0.99
88_Y	91_Y	1.014	0.99
171_A	348_L	1.012	0.99
114_T	122_T	1.012	0.99
6_K	10_T	1.012	0.99
55_Q	89_P	1.011	0.99
51_Q	55_Q	1.011	0.99
66_R	69_E	1.007	0.99
248_A	254_V	1.006	0.99
291_Q	294_K	1.004	0.99
302_V	350_G	0.998	0.99
166_T	252_D	0.997	0.99
337_A	341_Y	0.988	0.99
178_K	189_P	0.987	0.99
249_E	295_Y	0.986	0.99
322_S	326_R	0.986	0.99
307_T	350_G	0.985	0.99
248_A	297_K	0.985	0.99
8_Y	56_I	0.981	0.99
18_F	22_D	0.981	0.99
66_R	71_L	0.972	0.99
240_Y	244_G	0.967	0.99
10_T	14_A	0.966	0.99
310_T	350_G	0.964	0.99
18_F	26_L	0.958	0.99
87_V	138_R	0.952	0.99
318_K	322_S	0.951	0.99
67_F	74_F	0.951	0.99
321_L	328_F	0.943	0.98
36_L	39_R	0.941	0.98
286_D	289_K	0.935	0.98
12_R	64_R	0.934	0.98
51_Q	91_Y	0.934	0.98
258_D	304_P	0.934	0.98
16_I	64_R	0.930	0.98
59_R	140_V	0.929	0.98
302_V	351_S	0.927	0.98
19_Y	23_G	0.924	0.98
292_L	297_K	0.922	0.98
205_H	209_S	0.921	0.98
141_V	151_I	0.920	0.98
341_Y	344_S	0.920	0.98
144_T	230_E	0.914	0.98
255_V	347_I	0.908	0.98
233_K	237_T	0.906	0.98
272_L	276_L	0.905	0.98
45_Y	53_V	0.905	0.98
316_Y	332_N	0.904	0.98
247_F	254_V	0.902	0.98
81_H	84_Y	0.900	0.98
48_L	51_Q	0.900	0.98
261_V	282_S	0.898	0.98
207_E	210_R	0.896	0.98
325_D	328_F	0.895	0.98
92_G	118_S	0.893	0.98
11_L	31_L	0.893	0.98
178_K	187_Y	0.890	0.98
218_W	222_K	0.888	0.98
114_T	117_L	0.887	0.98
187_Y	341_Y	0.887	0.98
54_Q	58_D	0.883	0.98
231_H	235_L	0.881	0.98
67_F	75_P	0.880	0.98
309_K	317_V	0.879	0.98
240_Y	280_A	0.874	0.98
350_G	354_E	0.871	0.97
201_V	209_S	0.870	0.97
55_Q	59_R	0.870	0.97
202_K	205_H	0.868	0.97
312_A	335_W	0.867	0.97
205_H	229_Y	0.864	0.97
116_K	122_T	0.863	0.97
87_V	140_V	0.857	0.97
282_S	286_D	0.854	0.97
201_V	232_L	0.853	0.97
204_L	225_L	0.853	0.97
325_D	339_R	0.852	0.97
224_K	227_R	0.849	0.97
312_A	349_R	0.845	0.97
193_P	235_L	0.843	0.97
168_K	252_D	0.841	0.97
205_H	225_L	0.838	0.97
253_V	298_K	0.838	0.97
190_N	193_P	0.836	0.97
208_L	218_W	0.835	0.97
186_E	246_W	0.835	0.97
140_V	152_T	0.832	0.97
169_V	352_G	0.831	0.97
312_A	343_A	0.831	0.97
55_Q	87_V	0.828	0.97
211_K	217_N	0.822	0.96
327_V	337_A	0.822	0.96
112_L	117_L	0.822	0.96
13_W	77_E	0.821	0.96
39_R	43_D	0.819	0.96
345_L	348_L	0.819	0.96
180_I	243_L	0.817	0.96
310_T	346_N	0.816	0.96
202_K	206_R	0.816	0.96
13_W	16_I	0.816	0.96
326_R	338_D	0.809	0.96
170_V	183_S	0.806	0.96
197_A	201_V	0.806	0.96
258_D	285_R	0.802	0.96
239_L	243_L	0.802	0.96
35_A	54_Q	0.800	0.96
204_L	224_K	0.800	0.96
270_R	277_S	0.798	0.96
117_L	141_V	0.798	0.96
253_V	300_I	0.797	0.96
30_E	33_Q	0.795	0.96
343_A	346_N	0.791	0.96
127_I	130_D	0.788	0.95
228_A	232_L	0.787	0.95
15_D	61_Y	0.785	0.95
14_A	17_Y	0.781	0.95
174_V	180_I	0.778	0.95
170_V	254_V	0.778	0.95
176_I	265_I	0.777	0.95
181_V	188_F	0.777	0.95
245_K	291_Q	0.774	0.95
304_P	307_T	0.766	0.95
255_V	300_I	0.762	0.95
176_I	275_R	0.760	0.94
138_R	154_V	0.760	0.94
93_W	97_Q	0.757	0.94
60_Y	64_R	0.757	0.94
328_F	339_R	0.750	0.94
165_N	168_K	0.749	0.94
310_T	319_E	0.748	0.94
64_R	68_F	0.747	0.94
177_E	181_V	0.746	0.94
51_Q	54_Q	0.743	0.94
15_D	18_F	0.741	0.94
319_E	328_F	0.741	0.94
180_I	284_L	0.732	0.93
217_N	220_K	0.732	0.93
22_D	25_G	0.730	0.93
321_L	339_R	0.729	0.93
64_R	67_F	0.728	0.93
194_Y	198_L	0.728	0.93
309_K	318_K	0.728	0.93
117_L	123_F	0.727	0.93
115_L	139_V	0.726	0.93
188_F	243_L	0.724	0.93
201_V	205_H	0.724	0.93
306_Y	350_G	0.718	0.93
243_L	246_W	0.716	0.93
66_R	70_G	0.716	0.93
222_K	226_A	0.715	0.92
215_S	218_W	0.713	0.92
190_N	279_V	0.713	0.92
14_A	18_F	0.707	0.92
31_L	34_L	0.705	0.92
342_N	346_N	0.700	0.92
313_R	345_L	0.699	0.92
300_I	351_S	0.697	0.92
254_V	288_I	0.695	0.91
263_Q	304_P	0.695	0.91
113_I	116_K	0.693	0.91
179_L	279_V	0.693	0.91
203_H	207_E	0.693	0.91
169_V	255_V	0.690	0.91
51_Q	92_G	0.688	0.91
209_S	225_L	0.687	0.91
249_E	253_V	0.686	0.91
261_V	281_F	0.685	0.91
180_I	188_F	0.678	0.90
116_K	120_L	0.673	0.90
184_D	251_Y	0.668	0.90
135_K	156_E	0.668	0.90
175_G	181_V	0.667	0.90
148_R	234_N	0.665	0.90
194_Y	197_A	0.662	0.89
255_V	348_L	0.660	0.89
167_G	252_D	0.659	0.89
25_G	31_L	0.655	0.89
8_Y	60_Y	0.651	0.89
39_R	45_Y	0.648	0.88
20_Q	23_G	0.647	0.88
197_A	231_H	0.646	0.88
10_T	13_W	0.646	0.88
57_A	61_Y	0.646	0.88
349_R	352_G	0.645	0.88
263_Q	276_L	0.644	0.88
17_Y	20_Q	0.643	0.88
208_L	211_K	0.641	0.88
53_V	56_I	0.640	0.88
308_S	321_L	0.638	0.88
247_F	251_Y	0.638	0.88
26_L	31_L	0.636	0.87
206_R	210_R	0.634	0.87
93_W	136_V	0.634	0.87
36_L	50_S	0.634	0.87
292_L	295_Y	0.632	0.87
207_E	221_A	0.632	0.87
206_R	209_S	0.631	0.87
202_K	209_S	0.631	0.87
18_F	24_K	0.630	0.87
7_I	53_V	0.630	0.87
28_Q	31_L	0.629	0.87
168_K	184_D	0.628	0.87
197_A	228_A	0.628	0.87
16_I	19_Y	0.627	0.87
49_Y	88_Y	0.627	0.87
19_Y	64_R	0.624	0.86
50_S	92_G	0.623	0.86
320_D	325_D	0.623	0.86
52_V	86_L	0.623	0.86
91_Y	94_K	0.622	0.86
24_K	30_E	0.622	0.86
309_K	322_S	0.622	0.86
7_I	56_I	0.621	0.86
86_L	90_Q	0.621	0.86
69_E	72_A	0.619	0.86
25_G	61_Y	0.618	0.86
259_I	304_P	0.618	0.86
306_Y	310_T	0.617	0.86
121_G	124_K	0.617	0.86
163_L	166_T	0.616	0.86
290_Y	294_K	0.609	0.85
120_L	123_F	0.607	0.85
349_R	354_E	0.605	0.85
15_D	25_G	0.605	0.85
168_K	251_Y	0.604	0.85
306_Y	317_V	0.602	0.84
218_W	225_L	0.601	0.84
265_I	270_R	0.596	0.84
232_L	278_D	0.595	0.84
171_A	255_V	0.593	0.83
85_S	140_V	0.591	0.83
11_L	35_A	0.591	0.83
35_A	39_R	0.590	0.83
39_R	42_D	0.590	0.83
4_A	86_L	0.589	0.83
36_L	57_A	0.589	0.83
48_L	53_V	0.587	0.83
207_E	224_K	0.585	0.83
193_P	197_A	0.585	0.83
4_A	48_L	0.583	0.82
58_D	89_P	0.583	0.82
273_R	277_S	0.581	0.82
179_L	240_Y	0.581	0.82
90_Q	93_W	0.581	0.82
287_L	291_Q	0.581	0.82
280_A	283_E	0.580	0.82
10_T	44_E	0.580	0.82
229_Y	236_R	0.577	0.82
188_F	246_W	0.577	0.82
337_A	342_N	0.575	0.82
191_L	239_L	0.574	0.81
57_A	60_Y	0.574	0.81
50_S	89_P	0.574	0.81
93_W	139_V	0.572	0.81
308_S	322_S	0.571	0.81
319_E	322_S	0.570	0.81
316_Y	328_F	0.569	0.81
15_D	22_D	0.567	0.81
188_F	239_L	0.567	0.81
89_P	93_W	0.567	0.81
117_L	120_L	0.567	0.81
36_L	40_K	0.567	0.81
34_L	38_L	0.566	0.81
96_L	116_K	0.566	0.81
184_D	250_H	0.565	0.80
310_T	315_G	0.563	0.80
321_L	326_R	0.562	0.80
285_R	289_K	0.559	0.80
196_K	199_W	0.558	0.80
263_Q	272_L	0.558	0.80
92_G	95_I	0.557	0.80
144_T	149_I	0.555	0.79
173_D	340_D	0.555	0.79
347_I	350_G	0.555	0.79
10_T	38_L	0.554	0.79
288_I	299_L	0.554	0.79
14_A	31_L	0.554	0.79
210_R	221_A	0.553	0.79
195_E	274_R	0.552	0.79
32_R	36_L	0.552	0.79
251_Y	254_V	0.550	0.79
242_K	245_K	0.549	0.79
305_A	309_K	0.549	0.79
243_L	284_L	0.547	0.78
10_T	31_L	0.546	0.78
313_R	337_A	0.545	0.78
6_K	42_D	0.545	0.78
115_L	118_S	0.543	0.78
125_V	128_H	0.542	0.78
195_E	198_L	0.541	0.78
88_Y	93_W	0.541	0.78
237_T	241_M	0.541	0.78
129_R	132_P	0.541	0.78
265_I	271_S	0.540	0.77
15_D	19_Y	0.540	0.77
56_I	60_Y	0.539	0.77
323_L	338_D	0.539	0.77
245_K	248_A	0.539	0.77
177_E	326_R	0.536	0.77
177_E	338_D	0.535	0.77
323_L	327_V	0.534	0.77
229_Y	233_K	0.534	0.77
272_L	275_R	0.531	0.76
310_T	328_F	0.531	0.76
264_L	276_L	0.530	0.76
15_D	64_R	0.529	0.76
49_Y	90_Q	0.528	0.76
171_A	344_S	0.528	0.76
163_L	297_K	0.527	0.76
54_Q	57_A	0.525	0.75
88_Y	117_L	0.525	0.75
27_T	32_R	0.525	0.75
234_N	238_D	0.524	0.75
236_R	278_D	0.523	0.75
29_T	58_D	0.522	0.75
183_S	341_Y	0.521	0.75
318_K	339_R	0.521	0.75
171_A	183_S	0.521	0.75
25_G	30_E	0.521	0.75
325_D	331_S	0.519	0.75
329_V	335_W	0.519	0.75
144_T	150_Y	0.518	0.74
130_D	134_D	0.518	0.74
175_G	178_K	0.514	0.74
63_A	75_P	0.513	0.74
178_K	338_D	0.512	0.74
261_V	277_S	0.510	0.73
195_E	199_W	0.510	0.73
195_E	275_R	0.509	0.73
218_W	221_A	0.508	0.73
13_W	20_Q	0.506	0.73
49_Y	89_P	0.506	0.73
79_K	82_K	0.505	0.73
194_Y	274_R	0.505	0.73
8_Y	63_A	0.504	0.72
11_L	38_L	0.504	0.72
14_A	38_L	0.502	0.72
30_E	34_L	0.502	0.72
173_D	308_S	0.500	0.72

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1vhxA	2	0.3644	94.7	0.927	Contact Map
1nu0A	1	0.3404	94.1	0.929	Contact Map
1iv0A	1	0.2527	90	0.937	Contact Map
1ffkW	1	0.1676	89.4	0.938	Contact Map
4aybP	1	0.1144	87.5	0.94	Contact Map
1vq8Z	1	0.1941	84.1	0.943	Contact Map
1qypA	1	0.1223	83.2	0.943	Contact Map
1vd4A	1	0.1489	79.3	0.946	Contact Map
3j20Y	1	0.133	79.3	0.946	Contact Map
2k4xA	1	0.1463	78.8	0.946	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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