Q97X97 - SULSO - GREMLIN Contacts

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Q97X97 - Uncharacterized protein

UniProt:	Q97X97	Sequences: MKILAVYNHPVETLGTLKKFLPRVNEIMAEELKGDEQFEALIIMGGPMGVYERDKYHFLKVEIDLIKKGYKENKRILGVCLGSQLIAEALGGKVIKGGFGQEIGIQKVKLLGELKDFMREDELSVFQWHGDTFSLPQDAVLLAYSNNYFQAFKVKRALGVQFHLEVNSEMVSQWIKLYGGDESVANYVKRFEGELEDAAFKLIQYWMQL	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	209 (171)
Sequences:	10635 (7021)
Seq/√Len:	536.9

ECOLI - GUAA PABA TRPGD
BACSU - GUAA PABA
HALSA - Q9HMK6 TRPG GUAAA
SULSO - Q97X97 GUAAA TRPG

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
87_A	133_F	3.565	1.00
86_I	157_A	3.337	1.00
51_Y	88_E	2.804	1.00
142_L	153_K	2.720	1.00
87_A	152_F	2.584	1.00
141_L	149_F	2.457	1.00
120_E	171_V	2.401	1.00
171_V	174_W	2.353	1.00
107_K	122_E	2.343	1.00
93_K	134_S	2.307	1.00
140_V	153_K	2.249	1.00
109_K	122_E	2.113	1.00
111_L	144_Y	2.101	1.00
143_A	151_A	2.054	1.00
87_A	92_G	1.920	1.00
21_L	42_I	1.920	1.00
153_K	158_L	1.897	1.00
23_R	173_Q	1.879	1.00
107_K	124_S	1.877	1.00
83_S	159_G	1.872	1.00
5_A	17_L	1.867	1.00
123_L	171_V	1.857	1.00
141_L	144_Y	1.837	1.00
133_F	152_F	1.832	1.00
124_S	170_M	1.814	1.00
83_S	133_F	1.766	1.00
87_A	136_P	1.735	1.00
174_W	177_L	1.726	1.00
26_E	29_A	1.698	1.00
49_G	52_E	1.685	1.00
134_S	144_Y	1.667	1.00
118_M	151_A	1.611	1.00
135_L	141_L	1.597	1.00
26_E	30_E	1.593	1.00
152_F	159_G	1.528	1.00
27_I	30_E	1.524	1.00
66_I	85_L	1.514	1.00
46_G	81_L	1.504	1.00
109_K	144_Y	1.479	1.00
90_L	157_A	1.461	1.00
50_V	88_E	1.435	1.00
95_I	132_T	1.427	1.00
19_K	22_P	1.425	1.00
41_L	65_L	1.417	1.00
83_S	152_F	1.384	1.00
135_L	149_F	1.381	1.00
3_I	40_A	1.371	1.00
27_I	32_L	1.352	1.00
30_E	33_K	1.345	1.00
139_A	152_F	1.344	1.00
126_F	168_S	1.313	1.00
27_I	33_K	1.312	1.00
68_K	72_E	1.309	1.00
77_L	160_V	1.281	1.00
97_G	132_T	1.270	1.00
27_I	31_E	1.265	1.00
49_G	131_D	1.262	1.00
139_A	154_V	1.260	1.00
106_Q	126_F	1.258	1.00
138_D	154_V	1.256	1.00
101_Q	130_G	1.254	1.00
92_G	136_P	1.241	1.00
79_V	160_V	1.222	1.00
29_A	32_L	1.209	1.00
51_Y	131_D	1.206	1.00
41_L	74_K	1.204	1.00
86_I	159_G	1.185	1.00
48_M	52_E	1.169	1.00
68_K	71_K	1.164	1.00
59_L	85_L	1.161	1.00
123_L	175_I	1.161	1.00
115_K	153_K	1.134	1.00
35_D	38_F	1.129	1.00
101_Q	104_G	1.122	1.00
142_L	151_A	1.121	1.00
112_G	140_V	1.113	1.00
34_G	69_G	1.109	1.00
144_Y	148_Y	1.109	1.00
34_G	73_N	1.097	1.00
116_D	153_K	1.097	1.00
40_A	75_R	1.065	1.00
16_T	79_V	1.061	1.00
108_V	143_A	1.048	1.00
120_E	123_L	1.046	1.00
66_I	76_I	1.039	1.00
61_V	89_A	1.037	1.00
136_P	152_F	1.030	1.00
111_L	114_L	1.029	1.00
102_E	105_I	1.026	1.00
34_G	37_Q	1.025	1.00
4_L	40_A	1.020	1.00
117_F	158_L	1.015	1.00
102_E	145_S	1.009	1.00
29_A	33_K	1.000	1.00
34_G	72_E	0.980	1.00
16_T	176_K	0.973	1.00
142_L	158_L	0.969	1.00
138_D	155_K	0.967	1.00
106_Q	127_Q	0.960	1.00
18_K	22_P	0.952	1.00
118_M	162_F	0.947	1.00
109_K	146_N	0.935	1.00
97_G	148_Y	0.913	1.00
41_L	76_I	0.905	1.00
5_A	42_I	0.904	1.00
4_L	27_I	0.903	1.00
113_E	142_L	0.894	1.00
100_G	147_N	0.891	1.00
116_D	119_R	0.889	1.00
38_F	74_K	0.888	1.00
3_I	21_L	0.884	1.00
21_L	24_V	0.880	1.00
109_K	112_G	0.879	1.00
24_V	176_K	0.879	1.00
71_K	157_A	0.877	1.00
26_E	31_E	0.875	1.00
42_I	77_L	0.872	1.00
49_G	96_K	0.867	1.00
132_T	148_Y	0.867	1.00
28_M	33_K	0.859	1.00
170_M	173_Q	0.855	1.00
43_I	60_K	0.854	1.00
26_E	33_K	0.852	1.00
57_H	60_K	0.852	1.00
20_F	173_Q	0.851	1.00
116_D	158_L	0.850	1.00
19_K	23_R	0.847	1.00
76_I	86_I	0.846	1.00
114_L	142_L	0.840	1.00
57_H	85_L	0.817	1.00
20_F	24_V	0.814	1.00
106_Q	124_S	0.811	1.00
18_K	21_L	0.809	1.00
8_N	43_I	0.809	1.00
95_I	134_S	0.792	1.00
176_K	179_G	0.791	1.00
112_G	116_D	0.791	1.00
113_E	116_D	0.788	1.00
28_M	31_E	0.783	1.00
70_Y	157_A	0.780	1.00
56_Y	59_L	0.780	1.00
120_E	170_M	0.776	1.00
113_E	140_V	0.773	1.00
94_V	131_D	0.770	1.00
100_G	103_I	0.770	1.00
125_V	169_E	0.762	1.00
58_F	61_V	0.757	1.00
174_W	178_Y	0.757	1.00
92_G	133_F	0.748	1.00
59_L	89_A	0.739	1.00
66_I	86_I	0.739	1.00
90_L	154_V	0.732	1.00
102_E	150_Q	0.726	1.00
96_K	101_Q	0.725	1.00
60_K	68_K	0.725	1.00
41_L	69_G	0.724	1.00
43_I	76_I	0.713	1.00
160_V	164_L	0.709	1.00
57_H	61_V	0.709	1.00
48_M	51_Y	0.706	1.00
69_G	74_K	0.702	1.00
58_F	89_A	0.699	1.00
103_I	128_W	0.698	1.00
110_L	114_L	0.695	1.00
69_G	72_E	0.692	1.00
70_Y	90_L	0.688	1.00
80_C	84_Q	0.688	1.00
113_E	121_D	0.681	1.00
100_G	132_T	0.681	1.00
9_H	30_E	0.680	1.00
127_Q	150_Q	0.673	1.00
115_K	140_V	0.671	1.00
172_S	176_K	0.669	1.00
85_L	89_A	0.667	1.00
125_V	162_F	0.667	1.00
61_V	88_E	0.663	1.00
59_L	81_L	0.659	1.00
80_C	163_H	0.656	1.00
58_F	88_E	0.655	1.00
112_G	115_K	0.655	1.00
128_W	178_Y	0.654	1.00
88_E	93_K	0.654	1.00
173_Q	177_L	0.650	1.00
108_V	162_F	0.650	1.00
102_E	129_H	0.650	1.00
103_I	130_G	0.648	1.00
109_K	121_D	0.644	1.00
65_L	69_G	0.641	1.00
145_S	148_Y	0.640	1.00
115_K	142_L	0.633	1.00
83_S	87_A	0.631	1.00
49_G	88_E	0.630	1.00
56_Y	60_K	0.629	1.00
100_G	104_G	0.622	1.00
70_Y	76_I	0.616	1.00
9_H	12_E	0.615	1.00
133_F	149_F	0.614	1.00
126_F	166_V	0.611	1.00
134_S	137_Q	0.605	1.00
115_K	121_D	0.605	1.00
7_Y	13_T	0.604	1.00
88_E	94_V	0.600	1.00
26_E	32_L	0.600	1.00
20_F	23_R	0.598	1.00
132_T	149_F	0.595	1.00
14_L	17_L	0.593	1.00
105_I	126_F	0.591	1.00
114_L	153_K	0.590	1.00
168_S	172_S	0.587	0.99
96_K	131_D	0.587	0.99
96_K	130_G	0.584	0.99
110_L	122_E	0.583	0.99
52_E	56_Y	0.581	0.99
8_N	44_M	0.575	0.99
135_L	152_F	0.571	0.99
24_V	173_Q	0.570	0.99
151_A	160_V	0.569	0.99
92_G	134_S	0.566	0.99
6_V	65_L	0.559	0.99
36_E	74_K	0.556	0.99
4_L	38_F	0.556	0.99
14_L	18_K	0.556	0.99
84_Q	161_Q	0.555	0.99
135_L	139_A	0.551	0.99
123_L	170_M	0.547	0.99
151_A	158_L	0.544	0.99
167_N	170_M	0.544	0.99
24_V	177_L	0.544	0.99
90_L	139_A	0.540	0.99
105_I	124_S	0.540	0.99
61_V	68_K	0.539	0.99
95_I	98_G	0.538	0.99
112_G	121_D	0.534	0.99
7_Y	12_E	0.532	0.99
49_G	94_V	0.531	0.99
17_L	22_P	0.529	0.99
113_E	153_K	0.528	0.99
97_G	100_G	0.527	0.99
50_V	131_D	0.522	0.99
71_K	90_L	0.518	0.99
28_M	32_L	0.518	0.99
149_F	152_F	0.518	0.99
16_T	21_L	0.513	0.99
69_G	73_N	0.511	0.99
78_G	86_I	0.511	0.99
32_L	65_L	0.510	0.99
84_Q	94_V	0.510	0.99
43_I	66_I	0.510	0.99
19_K	173_Q	0.509	0.99
15_G	19_K	0.507	0.99
34_G	68_K	0.506	0.98
5_A	14_L	0.505	0.98
107_K	146_N	0.504	0.98
90_L	138_D	0.503	0.98
21_L	25_N	0.501	0.98
177_L	180_G	0.500	0.98

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3m3pA	1	0.9856	100	0.365	Contact Map
1o1yA	2	0.9952	100	0.365	Contact Map
3l7nA	1	0.9952	100	0.378	Contact Map
3tqiA	3	0.8852	100	0.407	Contact Map
1gpmA	3	0.9522	100	0.411	Contact Map
2vdjA	2	0.9904	100	0.418	Contact Map
3uowA	2	0.9665	100	0.422	Contact Map
2vxoA	2	0.933	100	0.426	Contact Map
3r75A	2	0.8708	100	0.427	Contact Map
2ywbA	4	0.9187	100	0.429	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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