Q97X39 - SULSO - GREMLIN Contacts

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Q97X39 - Second ORF in transposon ISC1913

UniProt:	Q97X39	Sequences: MERTVKLRVKVDYKTYSALKEVEKEYREVLEEAINYGLSNKTTSFTRIKAGVYKTEREKHKDLPSHYIYTACEDASERLDSFEKLKRRGRSYTEKPSVRRVTIHLDDHLWKFNLDRISISTKRSRILISPTFPKIFWRYYNKGWRIASEARFKLLKGNVVEFYVIFKRDEPKPYEPKGFISVDLNENSVSVLVDGKPMLLETNTKRITLGYEYRRKAITTGKSTKDREVRRKLKRLRERNKKVDIRRKLAKLIVKEAFESRSVIVLEDLPRRTPEHMIKDVKDSQLRLRIYRAAFSSMKNAIIEKAREFGVPVVLVNPSHTSTVCPVHGTKIVYQLDGGDAPRVGVCEKGKEKWHRDVVALYNLARRAGDVSPVPLGSKESHDPPKRWLRAKSLHSIMNDHKMIEMKV	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	408 (346)
Sequences:	4575 (3002)
Seq/√Len:	161.4

ECOLI - INSQ
HALSA - Q9HSW9 Q9HHJ9 Q9HSW7 Q9HM87 Q9HSZ6 Q9HHG8 Q9HSD4 Q9HHU6 Q9HSY4 Q9HHX8
SULSO - Q97X39 Q97WZ3 Q97XQ0 Q97U51 Q97XB6 Q97Y67 Q97YW9 Q97Z61 Q7LXH6 Q97YI5 Q97YU1 Q97UQ8 Q97YI2 Q97XQ8 Q97Y48 Q7LXD9 Q97XP1 Q97Y44 Q97V25 Q97U68 Q97V47 Q97YW4 Q7LXH2 Q97WY6 Q97V53 Q97XA4 Y2105 Q97YF7 Q97U09 Q7LX59 Q97YK6 Q7LXH9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
244_D	248_K	5.244	1.00
326_P	359_V	4.334	1.00
270_P	276_H	4.172	1.00
306_A	313_V	3.767	1.00
266_L	302_I	3.068	1.00
265_V	368_A	3.041	1.00
299_K	315_L	2.955	1.00
189_V	298_M	2.905	1.00
297_S	301_A	2.793	1.00
251_K	255_K	2.696	1.00
339_G	342_P	2.682	1.00
3_R	308_E	2.588	1.00
150_A	162_F	2.504	1.00
320_H	323_T	2.485	1.00
297_S	300_N	2.386	1.00
208_T	288_L	2.375	1.00
324_V	331_K	2.349	1.00
303_I	315_L	2.333	1.00
316_V	364_L	2.316	1.00
254_V	306_A	2.285	1.00
298_M	302_I	2.245	1.00
247_R	304_E	2.237	1.00
264_I	306_A	2.201	1.00
283_D	287_R	2.149	1.00
321_T	364_L	2.140	1.00
35_N	39_S	2.129	1.00
367_R	370_D	2.127	1.00
247_R	301_A	2.085	1.00
278_I	291_Y	2.075	1.00
207_I	241_K	2.074	1.00
147_A	167_K	2.063	1.00
346_V	353_K	2.056	1.00
12_D	15_T	2.048	1.00
38_L	95_K	2.023	1.00
229_V	233_L	2.004	1.00
323_T	333_V	1.983	1.00
323_T	338_G	1.974	1.00
266_L	313_V	1.962	1.00
20_K	154_L	1.953	1.00
33_A	72_C	1.933	1.00
328_H	350_G	1.924	1.00
34_I	75_A	1.883	1.00
214_R	229_V	1.880	1.00
243_V	297_S	1.863	1.00
246_R	301_A	1.840	1.00
250_A	301_A	1.838	1.00
343_R	356_R	1.831	1.00
284_S	287_R	1.831	1.00
325_C	328_H	1.830	1.00
247_R	305_K	1.824	1.00
328_H	347_C	1.824	1.00
247_R	250_A	1.789	1.00
189_V	245_I	1.785	1.00
262_S	311_V	1.769	1.00
21_E	62_D	1.741	1.00
227_R	230_R	1.739	1.00
20_K	160_V	1.730	1.00
327_V	352_E	1.720	1.00
182_V	190_S	1.715	1.00
303_I	307_R	1.709	1.00
325_C	347_C	1.703	1.00
347_C	350_G	1.702	1.00
8_R	159_V	1.688	1.00
345_G	354_W	1.674	1.00
325_C	350_G	1.657	1.00
246_R	297_S	1.603	1.00
217_A	221_G	1.586	1.00
19_L	160_V	1.583	1.00
266_L	315_L	1.579	1.00
267_E	318_P	1.531	1.00
224_T	227_R	1.510	1.00
332_I	338_G	1.493	1.00
266_L	299_K	1.479	1.00
23_E	160_V	1.475	1.00
17_S	21_E	1.458	1.00
338_G	342_P	1.457	1.00
345_G	352_E	1.454	1.00
85_L	93_T	1.442	1.00
191_V	358_V	1.434	1.00
192_L	196_K	1.420	1.00
74_D	111_K	1.418	1.00
188_S	191_V	1.418	1.00
268_D	315_L	1.412	1.00
221_G	229_V	1.403	1.00
86_K	93_T	1.403	1.00
362_Y	366_R	1.399	1.00
250_A	304_E	1.382	1.00
332_I	345_G	1.381	1.00
4_T	163_Y	1.377	1.00
33_A	75_A	1.369	1.00
218_I	230_R	1.356	1.00
179_F	365_A	1.354	1.00
16_Y	20_K	1.353	1.00
330_T	348_E	1.352	1.00
250_A	305_K	1.341	1.00
344_V	353_K	1.341	1.00
214_R	217_A	1.340	1.00
340_D	343_R	1.339	1.00
180_I	192_L	1.334	1.00
214_R	236_L	1.290	1.00
303_I	313_V	1.280	1.00
81_S	93_T	1.265	1.00
79_L	95_K	1.265	1.00
76_S	80_D	1.264	1.00
70_T	73_E	1.259	1.00
26_Y	71_A	1.243	1.00
290_I	295_F	1.238	1.00
332_I	348_E	1.233	1.00
254_V	309_F	1.233	1.00
207_I	238_E	1.226	1.00
358_V	362_Y	1.213	1.00
80_D	84_K	1.206	1.00
210_G	214_R	1.204	1.00
332_I	337_D	1.199	1.00
250_A	298_M	1.197	1.00
54_K	57_R	1.187	1.00
339_G	343_R	1.180	1.00
193_V	361_L	1.176	1.00
246_R	298_M	1.176	1.00
66_H	113_N	1.172	1.00
218_I	229_V	1.167	1.00
13_Y	16_Y	1.162	1.00
326_P	362_Y	1.160	1.00
326_P	354_W	1.156	1.00
182_V	192_L	1.143	1.00
46_T	49_K	1.138	1.00
179_F	263_V	1.128	1.00
215_R	219_T	1.126	1.00
181_S	365_A	1.121	1.00
271_R	296_S	1.121	1.00
251_K	309_F	1.119	1.00
66_H	114_L	1.116	1.00
270_P	318_P	1.115	1.00
77_E	81_S	1.114	1.00
339_G	356_R	1.112	1.00
184_L	295_F	1.101	1.00
301_A	304_E	1.097	1.00
53_Y	58_E	1.095	1.00
44_S	47_R	1.095	1.00
226_D	230_R	1.093	1.00
340_D	356_R	1.090	1.00
243_V	301_A	1.089	1.00
252_L	256_E	1.088	1.00
255_K	309_F	1.084	0.99
176_P	262_S	1.079	0.99
316_V	367_R	1.079	0.99
230_R	234_K	1.078	0.99
81_S	85_L	1.075	0.99
125_R	133_P	1.068	0.99
30_L	71_A	1.066	0.99
120_S	127_L	1.065	0.99
136_F	139_Y	1.065	0.99
55_T	59_K	1.063	0.99
155_L	159_V	1.058	0.99
81_S	86_K	1.058	0.99
126_I	162_F	1.057	0.99
354_W	362_Y	1.036	0.99
11_V	15_T	1.032	0.99
74_D	151_R	1.031	0.99
324_V	367_R	1.028	0.99
246_R	250_A	1.023	0.99
43_T	46_T	1.021	0.99
50_A	54_K	1.018	0.99
11_V	19_L	1.018	0.99
153_K	163_Y	1.014	0.99
197_P	355_H	1.014	0.99
179_F	369_G	1.009	0.99
305_K	308_E	1.001	0.99
254_V	311_V	1.000	0.99
321_T	367_R	1.000	0.99
128_I	152_F	0.996	0.99
34_I	79_L	0.994	0.99
250_A	302_I	0.992	0.99
256_E	261_R	0.991	0.99
330_T	349_K	0.989	0.99
186_E	293_A	0.984	0.99
212_E	216_K	0.980	0.99
28_E	32_E	0.976	0.99
268_D	318_P	0.976	0.99
64_P	67_Y	0.974	0.99
38_L	79_L	0.973	0.99
326_P	352_E	0.973	0.99
253_I	264_I	0.972	0.99
316_V	368_A	0.972	0.99
118_S	127_L	0.967	0.99
136_F	140_Y	0.963	0.99
278_I	283_D	0.961	0.99
184_L	299_K	0.958	0.99
44_S	48_I	0.957	0.99
263_V	312_P	0.948	0.99
6_K	155_L	0.940	0.99
211_Y	233_L	0.940	0.99
326_P	366_R	0.936	0.98
354_W	358_V	0.935	0.98
323_T	331_K	0.934	0.98
237_R	241_K	0.934	0.98
66_H	70_T	0.933	0.98
239_R	243_V	0.932	0.98
53_Y	57_R	0.928	0.98
180_I	193_V	0.924	0.98
338_G	345_G	0.923	0.98
82_F	95_K	0.919	0.98
69_Y	73_E	0.910	0.98
5_V	166_F	0.908	0.98
218_I	233_L	0.908	0.98
265_V	364_L	0.904	0.98
140_Y	144_W	0.903	0.98
190_S	249_L	0.902	0.98
358_V	361_L	0.900	0.98
211_Y	219_T	0.899	0.98
360_A	363_N	0.898	0.98
36_Y	40_N	0.897	0.98
343_R	355_H	0.895	0.98
254_V	264_I	0.889	0.98
82_F	96_P	0.887	0.98
51_G	54_K	0.887	0.98
36_Y	52_V	0.886	0.98
342_P	345_G	0.884	0.98
53_Y	56_E	0.878	0.98
113_N	116_R	0.874	0.98
37_G	79_L	0.872	0.98
85_L	94_E	0.870	0.98
333_V	337_D	0.869	0.98
203_N	241_K	0.867	0.98
342_P	356_R	0.861	0.97
300_N	303_I	0.858	0.97
318_P	321_T	0.858	0.97
57_R	68_I	0.857	0.97
306_A	311_V	0.857	0.97
30_L	75_A	0.855	0.97
296_S	300_N	0.852	0.97
44_S	49_K	0.851	0.97
63_L	66_H	0.851	0.97
326_P	360_A	0.847	0.97
198_M	245_I	0.847	0.97
112_F	115_D	0.846	0.97
45_F	49_K	0.846	0.97
152_F	162_F	0.845	0.97
212_E	215_R	0.836	0.97
37_G	76_S	0.835	0.97
211_Y	238_E	0.831	0.97
110_W	114_L	0.829	0.97
283_D	286_L	0.827	0.97
81_S	84_K	0.825	0.97
359_V	363_N	0.823	0.97
344_V	354_W	0.823	0.97
246_R	294_A	0.822	0.97
154_L	160_V	0.822	0.97
217_A	220_T	0.821	0.97
279_K	283_D	0.816	0.96
106_D	110_W	0.814	0.96
117_I	121_T	0.810	0.96
323_T	339_G	0.809	0.96
184_L	190_S	0.808	0.96
271_R	295_F	0.806	0.96
179_F	265_V	0.801	0.96
188_S	197_P	0.799	0.96
24_K	27_R	0.799	0.96
345_G	356_R	0.797	0.96
327_V	362_Y	0.797	0.96
203_N	207_I	0.797	0.96
187_N	191_V	0.796	0.96
191_V	197_P	0.795	0.96
41_K	79_L	0.794	0.96
332_I	339_G	0.791	0.96
9_V	162_F	0.790	0.96
223_S	226_D	0.788	0.96
214_R	233_L	0.788	0.96
30_L	78_R	0.787	0.96
16_Y	160_V	0.786	0.96
32_E	36_Y	0.785	0.96
207_I	211_Y	0.784	0.96
328_H	332_I	0.783	0.95
186_E	289_R	0.782	0.95
314_V	368_A	0.782	0.95
200_L	203_N	0.782	0.95
79_L	82_F	0.779	0.95
54_K	72_C	0.778	0.95
35_N	98_V	0.778	0.95
251_K	305_K	0.777	0.95
180_I	264_I	0.776	0.95
72_C	76_S	0.775	0.95
175_E	178_G	0.772	0.95
188_S	199_L	0.771	0.95
54_K	60_H	0.771	0.95
368_A	372_S	0.769	0.95
245_I	249_L	0.768	0.95
137_W	140_Y	0.768	0.95
269_L	318_P	0.765	0.95
110_W	113_N	0.765	0.95
362_Y	365_A	0.762	0.95
22_V	63_L	0.762	0.95
25_E	28_E	0.761	0.95
263_V	314_V	0.758	0.95
320_H	367_R	0.756	0.95
70_T	74_D	0.755	0.95
132_F	135_I	0.755	0.95
338_G	356_R	0.755	0.95
7_L	162_F	0.755	0.95
116_R	129_S	0.754	0.94
127_L	133_P	0.754	0.94
324_V	363_N	0.754	0.94
151_R	163_Y	0.750	0.94
40_N	44_S	0.748	0.94
177_K	262_S	0.748	0.94
215_R	233_L	0.746	0.94
117_I	129_S	0.745	0.94
110_W	119_I	0.745	0.94
330_T	333_V	0.743	0.94
337_D	342_P	0.740	0.94
66_H	115_D	0.739	0.94
29_V	71_A	0.738	0.94
225_K	228_E	0.735	0.94
102_T	108_H	0.734	0.94
24_K	28_E	0.733	0.94
108_H	111_K	0.732	0.94
8_R	161_E	0.730	0.93
178_G	262_S	0.729	0.93
286_L	290_I	0.727	0.93
36_Y	39_S	0.726	0.93
18_A	22_V	0.724	0.93
268_D	299_K	0.724	0.93
63_L	68_I	0.724	0.93
6_K	153_K	0.723	0.93
138_R	142_K	0.723	0.93
200_L	293_A	0.722	0.93
322_S	338_G	0.720	0.93
227_R	231_R	0.717	0.93
367_R	371_V	0.717	0.93
193_V	358_V	0.717	0.93
189_V	250_A	0.714	0.93
52_V	56_E	0.714	0.93
102_T	106_D	0.712	0.93
183_D	357_D	0.712	0.93
196_K	252_L	0.711	0.93
148_S	165_I	0.707	0.92
229_V	232_K	0.707	0.92
213_Y	217_A	0.704	0.92
323_T	332_I	0.704	0.92
284_S	291_Y	0.703	0.92
43_T	48_I	0.701	0.92
218_I	221_G	0.701	0.92
185_N	191_V	0.701	0.92
323_T	337_D	0.700	0.92
137_W	141_N	0.699	0.92
22_V	26_Y	0.699	0.92
131_T	134_K	0.697	0.92
304_E	308_E	0.694	0.92
138_R	141_N	0.693	0.92
178_G	261_R	0.692	0.92
66_H	69_Y	0.691	0.91
52_V	55_T	0.687	0.91
79_L	83_E	0.686	0.91
54_K	59_K	0.685	0.91
75_A	79_L	0.685	0.91
249_L	252_L	0.679	0.91
144_W	166_F	0.677	0.91
278_I	287_R	0.677	0.91
340_D	355_H	0.677	0.91
302_I	313_V	0.677	0.91
50_A	72_C	0.675	0.91
21_E	25_E	0.674	0.90
265_V	365_A	0.673	0.90
226_D	229_V	0.672	0.90
25_E	63_L	0.670	0.90
53_Y	69_Y	0.669	0.90
111_K	115_D	0.669	0.90
190_S	298_M	0.667	0.90
265_V	314_V	0.667	0.90
54_K	58_E	0.666	0.90
13_Y	17_S	0.666	0.90
338_G	343_R	0.665	0.90
128_I	132_F	0.665	0.90
71_A	75_A	0.660	0.90
82_F	85_L	0.658	0.89
221_G	225_K	0.653	0.89
330_T	345_G	0.653	0.89
159_V	240_N	0.653	0.89
238_E	292_R	0.651	0.89
189_V	293_A	0.643	0.88
255_K	263_V	0.643	0.88
207_I	237_R	0.642	0.88
264_I	302_I	0.641	0.88
204_T	207_I	0.637	0.88
243_V	247_R	0.636	0.88
126_I	129_S	0.633	0.87
242_K	292_R	0.633	0.87
204_T	208_T	0.633	0.87
112_F	116_R	0.630	0.87
41_K	82_F	0.629	0.87
190_S	198_M	0.629	0.87
14_K	17_S	0.628	0.87
333_V	338_G	0.625	0.87
72_C	75_A	0.624	0.87
322_S	339_G	0.619	0.86
354_W	359_V	0.616	0.86
320_H	331_K	0.616	0.86
36_Y	55_T	0.616	0.86
271_R	290_I	0.611	0.86
129_S	134_K	0.611	0.86
265_V	367_R	0.609	0.85
113_N	117_I	0.609	0.85
145_R	167_K	0.609	0.85
110_W	116_R	0.606	0.85
57_R	60_H	0.605	0.85
42_T	45_F	0.605	0.85
340_D	344_V	0.603	0.85
320_H	324_V	0.602	0.85
33_A	52_V	0.601	0.85
127_L	130_P	0.600	0.85
155_L	161_E	0.599	0.84
38_L	42_T	0.599	0.84
117_I	120_S	0.596	0.84
332_I	356_R	0.595	0.84
114_L	117_I	0.593	0.84
53_Y	60_H	0.590	0.84
192_L	198_M	0.590	0.84
35_N	38_L	0.590	0.84
22_V	64_P	0.588	0.83
214_R	232_K	0.588	0.83
70_T	111_K	0.587	0.83
216_K	219_T	0.584	0.83
6_K	243_V	0.584	0.83
140_Y	143_G	0.584	0.83
219_T	233_L	0.582	0.83
56_E	59_K	0.581	0.83
181_S	265_V	0.581	0.83
109_L	115_D	0.580	0.83
53_Y	72_C	0.579	0.82
119_I	128_I	0.579	0.82
317_N	320_H	0.577	0.82
306_A	309_F	0.577	0.82
205_K	289_R	0.575	0.82
11_V	16_Y	0.573	0.82
240_N	244_D	0.573	0.82
240_N	243_V	0.572	0.82
106_D	113_N	0.572	0.82
246_R	249_L	0.572	0.82
29_V	68_I	0.571	0.82
37_G	41_K	0.570	0.81
126_I	150_A	0.569	0.81
19_L	23_E	0.568	0.81
49_K	53_Y	0.567	0.81
106_D	109_L	0.566	0.81
194_D	198_M	0.565	0.81
4_T	165_I	0.565	0.81
50_A	53_Y	0.564	0.81
366_R	369_G	0.564	0.81
256_E	262_S	0.563	0.81
332_I	342_P	0.563	0.81
4_T	153_K	0.563	0.81
285_Q	289_R	0.562	0.81
43_T	47_R	0.558	0.80
301_A	305_K	0.557	0.80
36_Y	56_E	0.555	0.80
122_K	125_R	0.555	0.80
211_Y	215_R	0.554	0.80
107_D	111_K	0.553	0.80
56_E	60_H	0.553	0.80
369_G	372_S	0.552	0.79
198_M	252_L	0.551	0.79
233_L	236_L	0.550	0.79
83_E	86_K	0.549	0.79
348_E	356_R	0.544	0.78
157_G	234_K	0.543	0.78
346_V	352_E	0.543	0.78
187_N	343_R	0.542	0.78
41_K	45_F	0.541	0.78
205_K	208_T	0.541	0.78
322_S	332_I	0.541	0.78
276_H	318_P	0.540	0.78
286_L	289_R	0.540	0.78
296_S	299_K	0.539	0.78
26_Y	67_Y	0.538	0.78
68_I	71_A	0.538	0.78
18_A	21_E	0.538	0.78
287_R	291_Y	0.536	0.77
84_K	93_T	0.535	0.77
205_K	288_L	0.535	0.77
324_V	345_G	0.535	0.77
283_D	291_Y	0.534	0.77
214_R	218_I	0.534	0.77
32_E	59_K	0.534	0.77
294_A	297_S	0.533	0.77
230_R	233_L	0.533	0.77
338_G	341_A	0.533	0.77
204_T	288_L	0.532	0.77
37_G	40_N	0.529	0.76
106_D	112_F	0.528	0.76
283_D	290_I	0.527	0.76
197_P	358_V	0.527	0.76
183_D	322_S	0.526	0.76
324_V	329_G	0.525	0.76
276_H	279_K	0.522	0.76
52_V	57_R	0.522	0.76
33_A	56_E	0.522	0.76
276_H	290_I	0.519	0.75
248_K	251_K	0.519	0.75
178_G	194_D	0.519	0.75
238_E	242_K	0.519	0.75
173_P	176_P	0.515	0.75
278_I	285_Q	0.514	0.74
212_E	288_L	0.514	0.74
52_V	59_K	0.513	0.74
109_L	114_L	0.513	0.74
364_L	367_R	0.512	0.74
139_Y	142_K	0.510	0.74
50_A	57_R	0.510	0.74
215_R	218_I	0.508	0.74
110_W	129_S	0.508	0.74
137_W	142_K	0.506	0.73
355_H	360_A	0.505	0.73
154_L	161_E	0.504	0.73
181_S	193_V	0.504	0.73
21_E	24_K	0.503	0.73
128_I	131_T	0.502	0.73
181_S	361_L	0.502	0.73
210_G	236_L	0.501	0.73
230_R	236_L	0.500	0.72

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1nu0A	1	0.3186	93.3	0.933	Contact Map
1vhxA	2	0.3358	93	0.934	Contact Map
1iv0A	1	0.2377	88.3	0.941	Contact Map
1ffkW	1	0.1642	86.5	0.942	Contact Map
3j20Y	1	0.1225	84.3	0.944	Contact Map
1qypA	1	0.1201	84.2	0.944	Contact Map
1vq8Z	1	0.1716	82.9	0.945	Contact Map
4aybP	1	0.1078	82	0.946	Contact Map
2k4xA	1	0.1348	82	0.946	Contact Map
1vd4A	1	0.1446	72.9	0.95	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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