Q97WY1 - SULSO - GREMLIN Contacts

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Q97WY1 - Transposase ISC1332

UniProt:	Q97WY1	Sequences: MTKSKNNPGRGNTIRESNTMKVMEEIREKIRKAIKEGVSLREIEEIILQEAMMEEREAYLEVEDDHKNGTYFRYLGTGDGVLRLRVPRTRKGGFRPKILPEKYERTSPDYEDFLQQLVLSGMTPSQVKAVLAAKGIPYSEIVMNRVAERISNKLKEYKSRELPHDLLALYIDVKIVKVRISESIMERAIYIAIGVDLEGNKFVLDYEVRDREDLDGWKSFLSGLVSRGVSRVDVIVSDDFSGLDRVVSTLFPSSQHQLCITHMVRNLMRVLPDKEKEELMIRVRDLKSSRNVEEGRKAILSLSQLVQPFSPARAKRLLDAADKYCSFLNFPREIRHYLYTNNTSESFNLTLARFEEELGGYFPSLRSLEVYLYVSIHESNSRWKFRPMSVIRHYSYHLKQLHASRFQVSLDEDF	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	414 (366)
Sequences:	1231 (760)
Seq/√Len:	39.7

SULSO - Q97WY1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
174_K	352_A	4.563	1.00
303_S	318_L	4.222	1.00
173_V	188_A	4.151	1.00
171_I	221_L	4.115	1.00
292_V	296_R	4.108	1.00
196_D	202_F	3.975	1.00
282_R	305_L	3.960	1.00
177_K	186_E	3.765	1.00
351_L	372_L	3.664	1.00
237_S	256_H	3.304	1.00
255_Q	329_N	3.294	1.00
189_I	372_L	3.036	1.00
235_I	254_S	3.003	1.00
290_R	294_E	2.747	1.00
278_E	305_L	2.739	1.00
189_I	206_Y	2.722	1.00
271_L	279_L	2.615	1.00
169_L	232_V	2.569	1.00
191_I	348_N	2.543	1.00
279_L	302_L	2.500	1.00
132_A	137_P	2.468	1.00
315_K	319_D	2.431	1.00
291_N	294_E	2.426	1.00
346_S	350_T	2.381	1.00
178_V	368_L	2.355	1.00
300_L	318_L	2.345	1.00
268_M	280_M	2.340	1.00
35_K	38_V	2.284	1.00
298_A	301_S	2.272	1.00
207_E	223_G	2.218	0.99
62_V	66_H	2.196	0.99
232_V	254_S	2.194	0.99
168_A	334_I	2.110	0.99
282_R	306_V	2.096	0.99
261_T	265_R	2.077	0.99
164_H	230_S	2.051	0.99
349_L	360_G	2.028	0.99
273_D	276_K	2.011	0.99
32_K	35_K	1.991	0.99
176_V	368_L	1.978	0.99
153_K	156_E	1.969	0.99
307_Q	314_A	1.962	0.99
346_S	349_L	1.899	0.98
35_K	41_R	1.894	0.98
187_R	365_L	1.886	0.98
171_I	243_L	1.884	0.98
193_I	203_V	1.880	0.98
279_L	313_R	1.851	0.98
175_I	188_A	1.835	0.98
72_F	83_R	1.831	0.98
303_S	307_Q	1.828	0.98
47_I	60_L	1.819	0.98
167_L	194_G	1.775	0.97
302_L	314_A	1.761	0.97
132_A	135_G	1.726	0.97
76_G	81_V	1.722	0.97
224_L	229_V	1.710	0.97
47_I	56_R	1.706	0.97
263_M	324_Y	1.694	0.97
239_D	244_D	1.693	0.97
155_K	158_K	1.683	0.96
169_L	192_A	1.672	0.96
201_K	346_S	1.669	0.96
294_E	297_K	1.654	0.96
57_E	60_L	1.646	0.96
196_D	200_N	1.645	0.96
52_M	65_D	1.645	0.96
285_D	298_A	1.627	0.96
169_L	204_L	1.600	0.95
263_M	320_A	1.589	0.95
370_V	374_V	1.588	0.95
120_S	378_E	1.584	0.95
275_E	309_F	1.583	0.95
114_L	146_V	1.578	0.95
194_G	204_L	1.556	0.95
294_E	298_A	1.533	0.94
350_T	354_F	1.532	0.94
235_I	247_V	1.523	0.94
237_S	243_L	1.517	0.94
192_A	348_N	1.506	0.94
202_F	380_N	1.502	0.93
218_K	222_S	1.497	0.93
218_K	249_T	1.478	0.93
229_V	251_F	1.468	0.93
131_L	143_M	1.443	0.92
388_M	391_I	1.436	0.92
192_A	220_F	1.423	0.91
72_F	85_R	1.403	0.91
300_L	321_A	1.386	0.90
166_L	202_F	1.381	0.90
175_I	186_E	1.376	0.90
148_E	151_S	1.366	0.90
47_I	54_E	1.363	0.90
56_R	60_L	1.363	0.90
70_T	85_R	1.361	0.89
96_P	99_L	1.360	0.89
276_K	280_M	1.353	0.89
384_K	387_P	1.350	0.89
116_Q	120_S	1.339	0.89
275_E	310_S	1.338	0.89
290_R	332_R	1.336	0.89
124_P	143_M	1.333	0.88
307_Q	318_L	1.315	0.88
49_Q	56_R	1.309	0.88
48_L	89_T	1.306	0.87
236_V	338_L	1.301	0.87
71_Y	103_Y	1.298	0.87
263_M	316_R	1.287	0.87
191_I	351_L	1.260	0.85
292_V	325_C	1.250	0.85
123_T	359_G	1.247	0.85
214_L	249_T	1.247	0.85
53_M	57_E	1.246	0.85
45_E	56_R	1.240	0.85
141_I	145_R	1.222	0.84
368_L	372_L	1.222	0.84
54_E	60_L	1.215	0.83
41_R	44_E	1.213	0.83
41_R	47_I	1.209	0.83
169_L	235_I	1.199	0.82
303_S	314_A	1.196	0.82
301_S	304_Q	1.196	0.82
328_L	335_R	1.195	0.82
267_L	283_V	1.192	0.82
47_I	53_M	1.188	0.82
145_R	148_E	1.179	0.81
65_D	89_T	1.175	0.81
54_E	58_A	1.173	0.81
272_P	275_E	1.171	0.81
39_S	42_E	1.169	0.81
341_N	344_S	1.167	0.81
239_D	258_L	1.160	0.80
151_S	370_V	1.152	0.80
225_V	251_F	1.150	0.80
155_K	159_S	1.140	0.79
347_F	376_I	1.140	0.79
74_Y	81_V	1.137	0.79
300_L	304_Q	1.135	0.79
46_I	53_M	1.134	0.79
197_L	395_S	1.134	0.79
47_I	51_A	1.129	0.78
75_L	165_D	1.128	0.78
279_L	314_A	1.127	0.78
47_I	57_E	1.125	0.78
170_Y	195_V	1.122	0.78
351_L	376_I	1.118	0.78
350_T	353_R	1.116	0.78
71_Y	88_R	1.106	0.77
244_D	258_L	1.105	0.77
342_N	345_E	1.103	0.77
267_L	317_L	1.091	0.76
176_V	372_L	1.090	0.76
354_F	375_S	1.090	0.76
364_S	367_S	1.089	0.76
178_V	189_I	1.074	0.75
41_R	45_E	1.073	0.75
61_E	64_D	1.067	0.74
235_I	243_L	1.060	0.74
221_L	243_L	1.054	0.73
353_R	358_L	1.047	0.73
296_R	322_D	1.046	0.73
126_Q	353_R	1.042	0.73
237_S	244_D	1.040	0.72
118_V	355_E	1.036	0.72
347_F	351_L	1.030	0.72
389_S	392_R	1.026	0.71
244_D	323_K	1.025	0.71
153_K	377_H	1.023	0.71
313_R	316_R	1.015	0.71
78_G	107_S	1.015	0.71
214_L	245_R	1.010	0.70
56_R	59_Y	1.008	0.70
49_Q	55_E	1.005	0.70
253_S	322_D	1.003	0.70
180_I	187_R	1.000	0.69
149_R	309_F	1.000	0.69
191_I	347_F	0.999	0.69
327_F	337_Y	0.998	0.69
190_Y	211_R	0.996	0.69
54_E	57_E	0.996	0.69
47_I	59_Y	0.989	0.69
53_M	56_R	0.986	0.68
70_T	291_N	0.985	0.68
258_L	323_K	0.983	0.68
78_G	109_D	0.975	0.67
248_S	323_K	0.967	0.67
77_T	105_R	0.960	0.66
234_V	338_L	0.958	0.66
261_T	340_T	0.954	0.66
299_I	318_L	0.951	0.65
27_R	30_I	0.949	0.65
234_V	330_F	0.947	0.65
33_A	38_V	0.944	0.65
55_E	60_L	0.941	0.65
118_V	124_P	0.934	0.64
230_S	293_E	0.929	0.64
286_L	327_F	0.929	0.64
176_V	355_E	0.926	0.63
77_T	82_L	0.920	0.63
30_I	34_I	0.920	0.63
353_R	363_P	0.919	0.63
381_S	385_F	0.917	0.63
264_V	289_S	0.913	0.62
163_P	196_D	0.909	0.62
243_L	256_H	0.907	0.62
124_P	140_E	0.904	0.62
123_T	168_A	0.900	0.61
383_W	387_P	0.897	0.61
53_M	60_L	0.897	0.61
316_R	324_Y	0.896	0.61
354_F	371_Y	0.893	0.61
292_V	329_N	0.891	0.60
193_I	343_T	0.891	0.60
44_E	94_F	0.888	0.60
41_R	54_E	0.887	0.60
362_F	367_S	0.887	0.60
125_S	140_E	0.884	0.60
24_E	27_R	0.882	0.60
171_I	247_V	0.882	0.60
59_Y	64_D	0.882	0.60
232_V	235_I	0.874	0.59
268_M	276_K	0.872	0.59
261_T	280_M	0.872	0.59
67_K	80_G	0.871	0.59
370_V	373_Y	0.866	0.58
45_E	48_L	0.865	0.58
47_I	61_E	0.863	0.58
222_S	250_L	0.862	0.58
323_K	326_S	0.862	0.58
45_E	55_E	0.859	0.58
44_E	47_I	0.857	0.57
334_I	338_L	0.856	0.57
128_K	132_A	0.855	0.57
109_D	112_D	0.854	0.57
312_A	347_F	0.852	0.57
46_I	326_S	0.852	0.57
258_L	320_A	0.851	0.57
152_N	155_K	0.850	0.57
343_T	346_S	0.850	0.57
297_K	300_L	0.848	0.57
176_V	189_I	0.842	0.56
64_D	91_K	0.842	0.56
113_F	131_L	0.842	0.56
105_R	327_F	0.841	0.56
225_V	264_V	0.841	0.56
44_E	57_E	0.839	0.56
255_Q	326_S	0.838	0.56
54_E	59_Y	0.837	0.56
82_L	221_L	0.835	0.55
57_E	61_E	0.834	0.55
75_L	82_L	0.832	0.55
44_E	142_V	0.828	0.55
157_Y	380_N	0.827	0.55
38_V	42_E	0.825	0.54
143_M	309_F	0.825	0.54
251_F	274_K	0.823	0.54
168_A	236_V	0.822	0.54
387_P	392_R	0.820	0.54
348_N	351_L	0.819	0.54
41_R	53_M	0.818	0.54
344_S	347_F	0.817	0.54
191_I	258_L	0.816	0.54
304_Q	308_P	0.816	0.54
133_A	136_I	0.815	0.54
58_A	63_E	0.813	0.53
171_I	246_V	0.813	0.53
133_A	251_F	0.810	0.53
203_V	380_N	0.809	0.53
264_V	268_M	0.807	0.53
387_P	390_V	0.797	0.52
296_R	318_L	0.796	0.52
203_V	347_F	0.790	0.51
35_K	82_L	0.790	0.51
57_E	63_E	0.788	0.51
208_V	369_E	0.781	0.50
175_I	352_A	0.776	0.50
51_A	60_L	0.775	0.50
349_L	352_A	0.774	0.50
114_L	138_Y	0.773	0.50
160_R	202_F	0.773	0.50
138_Y	165_D	0.768	0.49
268_M	341_N	0.765	0.49
278_E	282_R	0.765	0.49
35_K	47_I	0.762	0.49
54_E	63_E	0.759	0.48
118_V	371_Y	0.759	0.48
315_K	322_D	0.759	0.48
179_R	363_P	0.758	0.48
65_D	91_K	0.756	0.48
354_F	359_G	0.756	0.48
30_I	47_I	0.752	0.48
349_L	359_G	0.749	0.48
171_I	251_F	0.749	0.48
97_K	344_S	0.748	0.47
255_Q	330_F	0.747	0.47
45_E	49_Q	0.747	0.47
364_S	371_Y	0.745	0.47
178_V	302_L	0.741	0.47
171_I	192_A	0.738	0.47
201_K	385_F	0.732	0.46
32_K	143_M	0.732	0.46
190_Y	209_R	0.732	0.46
392_R	395_S	0.727	0.46
156_E	297_K	0.726	0.45
188_A	211_R	0.725	0.45
53_M	67_K	0.723	0.45
391_I	395_S	0.722	0.45
321_A	325_C	0.720	0.45
140_E	149_R	0.718	0.45
261_T	268_M	0.718	0.45
201_K	350_T	0.716	0.45
94_F	98_I	0.715	0.44
128_K	140_E	0.712	0.44
138_Y	142_V	0.710	0.44
53_M	59_Y	0.708	0.44
387_P	391_I	0.706	0.44
30_I	35_K	0.705	0.44
103_Y	378_E	0.702	0.43
120_S	129_A	0.700	0.43
253_S	308_P	0.700	0.43
196_D	220_F	0.698	0.43
283_V	340_T	0.697	0.43
60_L	63_E	0.697	0.43
200_N	330_F	0.695	0.43
117_L	385_F	0.695	0.43
32_K	297_K	0.694	0.43
117_L	390_V	0.693	0.42
99_L	138_Y	0.688	0.42
124_P	179_R	0.688	0.42
74_Y	83_R	0.688	0.42
44_E	49_Q	0.687	0.42
388_M	392_R	0.685	0.42
174_K	355_E	0.685	0.42
117_L	295_G	0.684	0.42
201_K	349_L	0.683	0.42
171_I	235_I	0.680	0.41
142_V	145_R	0.680	0.41
214_L	240_F	0.679	0.41
381_S	384_K	0.678	0.41
75_L	163_P	0.676	0.41
177_K	245_R	0.675	0.41
270_V	312_A	0.674	0.41
56_R	143_M	0.673	0.41
361_Y	368_L	0.671	0.41
218_K	250_L	0.670	0.40
45_E	53_M	0.670	0.40
189_I	368_L	0.668	0.40
168_A	375_S	0.668	0.40
59_Y	63_E	0.668	0.40
181_S	192_A	0.662	0.40
59_Y	62_V	0.661	0.40
206_Y	272_P	0.659	0.39
30_I	41_R	0.659	0.39
373_Y	381_S	0.658	0.39
71_Y	358_L	0.657	0.39
335_R	339_Y	0.657	0.39
56_R	64_D	0.655	0.39
77_T	80_G	0.655	0.39
45_E	70_T	0.655	0.39
363_P	367_S	0.654	0.39
389_S	395_S	0.652	0.39
75_L	84_L	0.652	0.39
71_Y	86_V	0.650	0.39
275_E	278_E	0.649	0.39
213_D	360_G	0.649	0.39
205_D	220_F	0.648	0.39
122_M	249_T	0.646	0.38
327_F	330_F	0.645	0.38
26_I	33_A	0.645	0.38
213_D	306_V	0.641	0.38
304_Q	322_D	0.639	0.38
130_V	133_A	0.639	0.38
155_K	366_R	0.639	0.38
76_G	106_T	0.636	0.37
106_T	142_V	0.634	0.37
60_L	64_D	0.634	0.37
114_L	154_L	0.634	0.37
35_K	42_E	0.634	0.37
40_L	356_E	0.634	0.37
289_S	328_L	0.633	0.37
213_D	241_S	0.629	0.37
171_I	348_N	0.629	0.37
84_L	99_L	0.627	0.37
279_L	305_L	0.627	0.37
243_L	295_G	0.625	0.37
51_A	54_E	0.624	0.36
206_Y	369_E	0.624	0.36
270_V	316_R	0.624	0.36
269_R	333_E	0.623	0.36
229_V	303_S	0.622	0.36
34_I	47_I	0.621	0.36
157_Y	340_T	0.620	0.36
208_V	365_L	0.620	0.36
98_I	146_V	0.616	0.36
254_S	315_K	0.615	0.36
210_D	352_A	0.615	0.36
175_I	209_R	0.615	0.36
266_N	269_R	0.614	0.36
32_K	186_E	0.613	0.36
48_L	51_A	0.613	0.36
191_I	206_Y	0.610	0.35
110_Y	174_K	0.610	0.35
46_I	54_E	0.607	0.35
375_S	379_S	0.607	0.35
40_L	94_F	0.607	0.35
43_I	46_I	0.606	0.35
118_V	188_A	0.605	0.35
83_R	296_R	0.604	0.35
349_L	358_L	0.603	0.35
202_F	379_S	0.599	0.34
182_E	360_G	0.599	0.34
162_L	169_L	0.598	0.34
190_Y	339_Y	0.598	0.34
180_I	185_M	0.597	0.34
173_V	363_P	0.597	0.34
157_Y	220_F	0.596	0.34
71_Y	140_E	0.595	0.34
383_W	388_M	0.595	0.34
152_N	156_E	0.595	0.34
260_I	340_T	0.593	0.34
29_K	47_I	0.591	0.34
130_V	173_V	0.590	0.34
299_I	303_S	0.590	0.34
94_F	170_Y	0.589	0.34
243_L	380_N	0.587	0.33
126_Q	336_H	0.587	0.33
278_E	281_I	0.586	0.33
94_F	217_W	0.585	0.33
150_I	237_S	0.585	0.33
124_P	144_N	0.584	0.33
47_I	63_E	0.583	0.33
290_R	377_H	0.583	0.33
188_A	351_L	0.582	0.33
274_K	284_R	0.582	0.33
245_R	248_S	0.582	0.33
379_S	383_W	0.581	0.33
279_L	306_V	0.580	0.33
120_S	225_V	0.580	0.33
360_G	371_Y	0.578	0.33
44_E	48_L	0.578	0.33
124_P	141_I	0.577	0.33
225_V	230_S	0.576	0.33
302_L	313_R	0.573	0.32
239_D	344_S	0.572	0.32
104_E	332_R	0.569	0.32
296_R	300_L	0.569	0.32
283_V	287_K	0.567	0.32
299_I	341_N	0.567	0.32
92_G	98_I	0.563	0.31
260_I	327_F	0.563	0.31
212_E	262_H	0.562	0.31
216_G	223_G	0.561	0.31
84_L	329_N	0.561	0.31
158_K	293_E	0.561	0.31
30_I	33_A	0.560	0.31
147_A	321_A	0.560	0.31
248_S	281_I	0.559	0.31
351_L	354_F	0.558	0.31
171_I	237_S	0.557	0.31
225_V	250_L	0.556	0.31
346_S	371_Y	0.555	0.31
40_L	47_I	0.553	0.31
302_L	306_V	0.552	0.31
66_H	91_K	0.552	0.31
157_Y	162_L	0.552	0.31
83_R	247_V	0.551	0.31
31_R	47_I	0.551	0.31
116_Q	130_V	0.550	0.30
128_K	131_L	0.549	0.30
187_R	216_G	0.548	0.30
218_K	246_V	0.548	0.30
206_Y	365_L	0.548	0.30
267_L	340_T	0.548	0.30
102_K	157_Y	0.547	0.30
133_A	367_S	0.546	0.30
214_L	252_P	0.546	0.30
95_R	102_K	0.546	0.30
147_A	287_K	0.542	0.30
99_L	103_Y	0.542	0.30
119_L	335_R	0.542	0.30
123_T	383_W	0.541	0.30
157_Y	370_V	0.541	0.30
162_L	166_L	0.540	0.30
56_R	67_K	0.539	0.30
250_L	254_S	0.538	0.30
330_F	338_L	0.538	0.30
41_R	60_L	0.537	0.29
333_E	393_H	0.535	0.29
91_K	198_E	0.535	0.29
30_I	38_V	0.533	0.29
237_S	247_V	0.532	0.29
360_G	375_S	0.532	0.29
390_V	393_H	0.532	0.29
90_R	224_L	0.531	0.29
181_S	395_S	0.531	0.29
34_I	40_L	0.529	0.29
235_I	246_V	0.528	0.29
348_N	353_R	0.527	0.29
173_V	240_F	0.527	0.29
171_I	306_V	0.526	0.29
163_P	338_L	0.526	0.29
154_L	369_E	0.525	0.29
323_K	367_S	0.525	0.29
269_R	287_K	0.524	0.28
143_M	177_K	0.523	0.28
239_D	256_H	0.523	0.28
106_T	368_L	0.522	0.28
123_T	313_R	0.522	0.28
196_D	206_Y	0.522	0.28
177_K	195_V	0.521	0.28
235_I	343_T	0.520	0.28
198_E	372_L	0.520	0.28
154_L	216_G	0.519	0.28
123_T	126_Q	0.519	0.28
286_L	346_S	0.518	0.28
123_T	361_Y	0.517	0.28
350_T	371_Y	0.517	0.28
194_G	311_P	0.517	0.28
34_I	314_A	0.516	0.28
154_L	329_N	0.515	0.28
127_V	265_R	0.515	0.28
68_N	217_W	0.514	0.28
358_L	371_Y	0.514	0.28
260_I	324_Y	0.514	0.28
163_P	366_R	0.514	0.28
73_R	157_Y	0.514	0.28
125_S	350_T	0.514	0.28
93_G	367_S	0.513	0.28
201_K	299_I	0.513	0.28
29_K	32_K	0.513	0.28
271_L	310_S	0.511	0.28
363_P	371_Y	0.510	0.27
97_K	269_R	0.509	0.27
86_V	103_Y	0.509	0.27
34_I	57_E	0.508	0.27
115_Q	330_F	0.507	0.27
117_L	122_M	0.507	0.27
193_I	201_K	0.507	0.27
106_T	150_I	0.505	0.27
39_S	175_I	0.505	0.27
265_R	341_N	0.503	0.27
45_E	85_R	0.502	0.27
78_G	106_T	0.502	0.27
72_F	230_S	0.502	0.27
266_N	322_D	0.502	0.27
30_I	42_E	0.501	0.27
146_V	164_H	0.501	0.27
229_V	232_V	0.500	0.27
184_I	361_Y	0.500	0.27

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4u7bA	2	0.6884	97.6	0.939	Contact Map
1bcoA	1	0.4758	90.2	0.961	Contact Map
4fcyA	4	0.756	85.1	0.964	Contact Map
2bw3A	3	0.6522	82.1	0.965	Contact Map
1tc3C	1	0.1232	75.8	0.968	Contact Map
3frwA	2	0.2271	68.4	0.969	Contact Map
2x48A	1	0.1304	58	0.971	Contact Map
1reqA	1	0.6498	55.3	0.972	Contact Map
1jkoC	1	0.1111	55.1	0.972	Contact Map
1on0A	4	0.3454	52.6	0.972	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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