GREMLIN Database
CAS6B - CRISPR-associated endoribonuclease Cas6 2
UniProt: Q97WV8 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 289 (246)
Sequences: 209 (184)
Seq/√Len: 11.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
177_I243_F4.3841.00
54_F86_A4.1971.00
125_L128_E3.5610.98
110_F114_I3.3260.98
168_Y268_R3.1920.97
9_Y85_I3.0560.96
107_Y114_I2.9900.95
107_Y110_F2.8660.94
266_I277_I2.7930.93
24_S57_H2.5650.90
127_E130_E2.5200.89
103_F107_Y2.4380.87
81_L89_T2.3580.85
56_S276_E2.3460.85
112_I115_E2.2680.83
184_V270_R2.2610.83
11_V58_L2.2000.81
16_D77_K2.1380.79
181_A266_I2.1080.78
108_G114_I2.1050.78
124_K128_E2.0190.75
28_K32_Q2.0120.74
84_I243_F2.0120.74
141_F145_T1.9660.72
267_G273_G1.9380.71
267_G275_G1.9380.71
239_G273_G1.9380.71
239_G275_G1.9380.71
239_G267_G1.9380.71
58_L78_G1.9160.70
34_G41_K1.8780.69
269_S272_I1.8740.69
45_T147_L1.8680.68
127_E131_K1.8150.66
181_A264_L1.6710.59
104_E110_F1.6610.59
103_F110_F1.6550.59
104_E109_K1.6370.58
110_F117_I1.6340.58
245_I254_A1.6290.57
185_Y189_I1.6220.57
28_K31_I1.6180.57
25_K28_K1.6120.57
268_R272_I1.6080.56
112_I217_H1.6060.56
180_Y185_Y1.6050.56
106_V110_F1.5890.55
103_F109_K1.5760.55
220_T272_I1.5560.54
108_G112_I1.5550.54
258_L270_R1.5420.53
9_Y83_S1.5300.53
176_L243_F1.5220.52
12_I71_N1.4670.49
28_K270_R1.4570.49
18_I21_T1.4470.48
202_G264_L1.4400.48
88_S257_Y1.4210.47
205_S210_R1.4200.47
54_F278_R1.4150.47
45_T270_R1.4110.47
183_N204_L1.4030.46
144_P275_G1.4010.46
144_P267_G1.4010.46
144_P239_G1.4010.46
144_P273_G1.4010.46
62_Q65_I1.3870.45
86_A276_E1.3750.45
115_E120_V1.3160.42
86_A261_S1.3090.42
212_I218_P1.2930.41
138_R242_E1.2920.41
78_G139_V1.2920.41
110_F115_E1.2740.40
72_L77_K1.2720.40
13_P16_D1.2650.40
169_S173_S1.2620.39
56_S83_S1.2580.39
118_E121_E1.2550.39
45_T257_Y1.2520.39
147_L218_P1.2430.38
145_T237_V1.2200.37
12_I18_I1.2120.37
264_L270_R1.2070.37
112_I122_V1.1760.35
169_S174_V1.1640.35
12_I113_M1.1630.35
24_S28_K1.1620.35
17_V85_I1.1460.34
104_E112_I1.1430.34
265_G270_R1.1270.33
221_V227_S1.1260.33
17_V86_A1.0990.32
51_K109_K1.0870.31
34_G38_P1.0850.31
270_R277_I1.0830.31
181_A260_T1.0760.31
65_I77_K1.0740.31
28_K53_I1.0740.31
54_F88_S1.0700.31
77_K123_E1.0650.30
112_I118_E1.0640.30
24_S32_Q1.0630.30
85_I110_F1.0580.30
141_F172_P1.0560.30
12_I99_D1.0510.30
137_I209_S1.0420.30
239_G265_G1.0290.29
265_G273_G1.0290.29
265_G275_G1.0290.29
27_L31_I1.0260.29
132_H143_S1.0210.29
105_T119_I1.0210.29
109_K120_V1.0190.29
179_A266_I1.0140.28
139_V258_L1.0140.28
100_E108_G1.0070.28
185_Y257_Y1.0040.28
176_L226_D1.0030.28
26_V143_S1.0030.28
108_G143_S0.9910.27
213_G216_L0.9900.27
32_Q184_V0.9880.27
172_P239_G0.9870.27
172_P267_G0.9870.27
172_P273_G0.9870.27
172_P275_G0.9870.27
28_K272_I0.9800.27
147_L220_T0.9760.27
46_S141_F0.9700.27
226_D233_K0.9630.26
143_S236_G0.9570.26
97_V182_Y0.9540.26
31_I186_C0.9530.26
37_I225_E0.9520.26
26_V75_I0.9490.26
103_F114_I0.9460.26
262_S265_G0.9450.26
140_R146_L0.9450.26
187_N243_F0.9440.26
46_S227_S0.9440.26
146_L269_S0.9430.26
195_E205_S0.9410.26
41_K245_I0.9410.26
137_I201_F0.9390.25
87_F242_E0.9360.25
116_S196_V0.9280.25
170_T270_R0.9230.25
256_N270_R0.9140.25
265_G269_S0.9120.24
184_V269_S0.9090.24
20_P95_S0.9050.24
185_Y263_Y0.9040.24
270_R274_F0.9040.24
72_L111_H0.9020.24
117_I127_E0.9010.24
43_L46_S0.8910.24
147_L269_S0.8880.24
108_G115_E0.8870.24
185_Y260_T0.8790.23
203_I218_P0.8760.23
38_P41_K0.8760.23
42_D63_R0.8760.23
93_V103_F0.8710.23
117_I120_V0.8680.23
51_K265_G0.8630.23
13_P17_V0.8620.23
114_I121_E0.8600.23
108_G121_E0.8560.22
87_F200_K0.8500.22
28_K181_A0.8500.22
29_Y56_S0.8490.22
103_F108_G0.8490.22
53_I271_G0.8450.22
171_L243_F0.8350.22
262_S276_E0.8330.22
93_V100_E0.8320.22
98_A241_I0.8310.22
85_I102_I0.8270.21
10_N13_P0.8270.21
124_K129_V0.8270.21
33_S188_L0.8260.21
107_Y115_E0.8240.21
168_Y272_I0.8140.21
206_N211_I0.8120.21
168_Y269_S0.8110.21
243_F261_S0.8110.21
211_I227_S0.8090.21
220_T235_R0.8070.21
35_K132_H0.8030.21
81_L96_E0.8000.21
45_T88_S0.7990.21
116_S120_V0.7950.20
184_V260_T0.7950.20
39_S110_F0.7860.20
38_P176_L0.7860.20
88_S185_Y0.7850.20
236_G263_Y0.7800.20
106_V112_I0.7790.20
83_S279_L0.7790.20
19_L252_R0.7770.20
263_Y270_R0.7740.20
39_S139_V0.7730.20
109_K115_E0.7720.20
85_I221_V0.7710.20
114_I120_V0.7640.19
8_G84_I0.7620.19
208_L212_I0.7620.19
95_S243_F0.7610.19
39_S143_S0.7610.19
98_A117_I0.7600.19
225_E233_K0.7600.19
207_A212_I0.7540.19
103_F115_E0.7530.19
28_K265_G0.7510.19
248_E256_N0.7470.19
40_L163_K0.7460.19
77_K91_A0.7460.19
106_V115_E0.7440.19
163_K209_S0.7430.19
63_R143_S0.7410.19
30_L51_K0.7410.19
53_I97_V0.7370.19
9_Y54_F0.7350.19
23_S108_G0.7320.18
29_Y32_Q0.7220.18
16_D76_T0.7190.18
177_I195_E0.7150.18
10_N113_M0.7140.18
95_S103_F0.7120.18
104_E120_V0.7110.18
213_G218_P0.7100.18
202_G205_S0.7080.18
103_F120_V0.7060.18
44_I271_G0.7060.18
186_C189_I0.7050.18
30_L188_L0.7040.18
44_I53_I0.7040.18
211_I218_P0.7040.18
40_L268_R0.7000.18
264_L277_I0.6990.17
14_L91_A0.6960.17
87_F142_V0.6940.17
17_V21_T0.6900.17
67_Q73_K0.6850.17
115_E122_V0.6840.17
88_S139_V0.6840.17
34_G37_I0.6790.17
83_S97_V0.6780.17
140_R169_S0.6760.17
175_G235_R0.6740.17
10_N17_V0.6720.17
116_S121_E0.6690.17
24_S55_I0.6670.17
62_Q119_I0.6610.16
8_G278_R0.6610.16
40_L178_V0.6600.16
31_I259_L0.6600.16
180_Y186_C0.6560.16
7_I189_I0.6550.16
21_T24_S0.6510.16
60_F190_G0.6490.16
101_G104_E0.6460.16
36_L99_D0.6460.16
13_P278_R0.6440.16
265_G268_R0.6350.16
104_E278_R0.6340.16
19_L113_M0.6340.16
54_F213_G0.6320.16
216_L241_I0.6270.16
123_E238_M0.6250.15
28_K45_T0.6240.15
55_I165_H0.6230.15
88_S180_Y0.6220.15
87_F97_V0.6210.15
201_F264_L0.6200.15
104_E107_Y0.6160.15
206_N209_S0.6160.15
107_Y112_I0.6150.15
223_I227_S0.6140.15
56_S178_V0.6120.15
220_T227_S0.6100.15
118_E122_V0.6100.15
20_P224_G0.6090.15
102_I121_E0.6090.15
23_S57_H0.6080.15
185_Y270_R0.6080.15
8_G18_I0.6070.15
24_S27_L0.6070.15
205_S220_T0.6070.15
263_Y268_R0.6050.15
83_S163_K0.6000.15
140_R194_V0.6000.15
14_L133_M0.6000.15
55_I167_G0.5980.15
38_P58_L0.5970.15
146_L274_F0.5970.15
166_A186_C0.5970.15
170_T175_G0.5960.15
78_G140_R0.5930.15
85_I132_H0.5920.15
106_V109_K0.5910.15
43_L47_R0.5900.15
24_S89_T0.5900.15
99_D191_K0.5900.15
191_K278_R0.5870.15
170_T220_T0.5860.14
266_I272_I0.5840.14
177_I218_P0.5830.14
234_A243_F0.5820.14
181_A257_Y0.5800.14
76_T261_S0.5800.14
92_N274_F0.5790.14
189_I270_R0.5760.14
30_L34_G0.5750.14
181_A185_Y0.5750.14
9_Y15_Q0.5710.14
13_P180_Y0.5710.14
177_I261_S0.5690.14
47_R187_N0.5670.14
63_R66_F0.5640.14
106_V117_I0.5640.14
181_A268_R0.5610.14
31_I181_A0.5600.14
211_I220_T0.5540.14
65_I176_L0.5540.14
109_K114_I0.5520.14
38_P224_G0.5520.14
53_I225_E0.5470.14
40_L167_G0.5440.13
17_V110_F0.5440.13
178_V201_F0.5420.13
141_F166_A0.5400.13
168_Y274_F0.5380.13
15_Q247_D0.5380.13
178_V277_I0.5370.13
77_K207_A0.5350.13
101_G125_L0.5350.13
100_E104_E0.5330.13
225_E260_T0.5320.13
109_K118_E0.5310.13
45_T269_S0.5310.13
173_S237_V0.5310.13
88_S147_L0.5280.13
227_S233_K0.5280.13
81_L271_G0.5260.13
257_Y260_T0.5260.13
49_K122_V0.5260.13
186_C257_Y0.5230.13
20_P64_R0.5210.13
33_S250_L0.5210.13
181_A184_V0.5200.13
8_G137_I0.5200.13
111_H120_V0.5160.13
52_P182_Y0.5150.13
251_K254_A0.5140.13
135_D254_A0.5130.13
52_P132_H0.5130.13
22_P176_L0.5130.13
28_K147_L0.5120.13
29_Y181_A0.5120.13
224_G227_S0.5100.13
243_F247_D0.5100.13
235_R271_G0.5090.13
15_Q90_Q0.5060.13
103_F177_I0.5060.13
146_L265_G0.5030.13
22_P223_I0.5030.13
23_S223_I0.5000.12
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4illA 2 0.9619 100 0.364 Contact Map
4c98A 2 0.7716 100 0.564 Contact Map
4c97A 1 0.7647 100 0.595 Contact Map
3i4hX 1 0.7543 99.9 0.663 Contact Map
3ufcX 1 0.8028 99.9 0.676 Contact Map
4qtsA 1 0.5398 41.4 0.954 Contact Map
1wicA 1 0.4602 12.6 0.964 Contact Map
4w8zA 1 0.5087 10.5 0.966 Contact Map
4l6uA 1 0.4913 8.2 0.967 Contact Map
1mspA 3 0.4118 4.6 0.971 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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