Q97WR5 - SULSO - GREMLIN Contacts

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Q97WR5 - Uncharacterized protein

UniProt:	Q97WR5	Sequences: MEFLLMTIGLFISHGSPTILIDENKWKDLLRRVGKEIEEKYKPETIIVSSPHFVSWTETFYIESSEKLECIQDYYDFPDETYKYCYNALNDTELVEEIVKNAEGKVVKDDKWGLDHGAWIPLFYMFPEYKPKVVTISITDNSPESHYEIGEKIRKAVEKLGRKAVFLATGSPTHRLDLFYFKITPKPTKFDAILMDLIKSGEFEKILKISELYPKEYQTAMPEGNLNTLHMLLGYIKPKKAEILGYDTPWPGVSMLAASFYE	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	262 (245)
Sequences:	2059 (1458)
Seq/√Len:	93.1

ECOLI - YGID
SULSO - Q97WR5 Q97X42

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
97_E	159_K	7.143	1.00
79_D	83_K	5.339	1.00
6_M	158_E	3.770	1.00
42_K	130_K	3.737	1.00
202_E	205_K	3.277	1.00
44_E	163_K	3.137	1.00
189_K	215_K	2.901	1.00
64_S	108_K	2.824	1.00
148_E	151_E	2.819	1.00
144_E	148_E	2.748	1.00
226_L	230_H	2.635	1.00
208_K	211_E	2.559	1.00
45_T	164_A	2.548	1.00
91_D	134_V	2.483	1.00
23_E	28_D	2.458	1.00
8_I	157_V	2.436	1.00
210_S	217_Y	2.388	1.00
61_Y	109_D	2.313	1.00
71_I	113_G	2.239	1.00
142_S	145_S	2.169	1.00
47_I	136_I	2.153	1.00
46_I	131_P	2.148	1.00
172_P	194_L	2.146	1.00
166_F	235_Y	2.104	1.00
99_V	108_K	1.910	1.00
84_Y	123_F	1.909	1.00
92_T	95_V	1.900	1.00
178_L	218_Q	1.885	1.00
193_I	213_Y	1.817	1.00
49_S	231_M	1.816	1.00
139_T	146_H	1.816	1.00
194_L	226_L	1.794	1.00
122_L	133_V	1.788	1.00
47_I	166_F	1.738	1.00
60_F	139_T	1.733	1.00
101_N	155_K	1.716	1.00
62_I	99_V	1.699	1.00
168_A	232_L	1.677	1.00
147_Y	203_F	1.672	1.00
13_S	171_S	1.613	1.00
150_G	234_G	1.591	1.00
12_I	167_L	1.589	1.00
51_P	224_G	1.573	1.00
63_E	135_T	1.562	1.00
54_V	224_G	1.552	1.00
47_I	98_I	1.549	1.00
243_I	246_Y	1.532	1.00
72_Q	85_C	1.528	1.00
191_D	256_L	1.527	1.00
54_V	222_P	1.517	1.00
209_I	216_E	1.517	1.00
171_S	255_M	1.515	1.00
138_I	224_G	1.507	1.00
48_V	118_A	1.467	1.00
114_L	118_A	1.462	1.00
150_G	235_Y	1.442	1.00
197_L	205_K	1.424	0.99
96_E	100_K	1.411	0.99
9_G	235_Y	1.410	0.99
54_V	140_D	1.409	0.99
80_E	83_K	1.408	0.99
48_V	122_L	1.396	0.99
97_E	101_N	1.395	0.99
62_I	98_I	1.383	0.99
7_T	126_F	1.379	0.99
32_R	35_K	1.379	0.99
191_D	254_S	1.370	0.99
61_Y	107_V	1.366	0.99
98_I	134_V	1.357	0.99
59_T	139_T	1.353	0.99
9_G	232_L	1.333	0.99
74_Y	119_W	1.332	0.99
208_K	212_L	1.299	0.99
193_I	197_L	1.281	0.99
185_P	189_K	1.280	0.99
69_E	112_W	1.269	0.99
7_T	165_V	1.252	0.99
48_V	167_L	1.249	0.99
99_V	106_V	1.240	0.98
97_E	100_K	1.217	0.98
197_L	202_E	1.207	0.98
213_Y	216_E	1.201	0.98
87_N	129_Y	1.201	0.98
101_N	156_A	1.193	0.98
100_K	104_G	1.180	0.98
44_E	159_K	1.177	0.98
70_C	114_L	1.168	0.98
139_T	145_S	1.165	0.98
195_M	199_K	1.164	0.98
60_F	149_I	1.155	0.98
188_T	192_A	1.140	0.97
24_N	27_K	1.138	0.97
147_Y	207_L	1.133	0.97
151_E	203_F	1.128	0.97
89_L	92_T	1.125	0.97
90_N	95_V	1.097	0.97
201_G	204_E	1.096	0.97
98_I	101_N	1.089	0.97
154_R	237_K	1.076	0.96
191_D	195_M	1.075	0.96
138_I	227_N	1.072	0.96
147_Y	233_L	1.071	0.96
45_T	94_L	1.069	0.96
118_A	135_T	1.062	0.96
143_P	209_I	1.048	0.96
64_S	110_D	1.039	0.95
20_L	123_F	1.028	0.95
47_I	134_V	1.022	0.95
146_H	231_M	1.021	0.95
114_L	133_V	1.015	0.95
26_W	247_D	1.015	0.95
70_C	88_A	1.008	0.95
62_I	108_K	1.002	0.95
183_I	188_T	0.997	0.94
91_D	94_L	0.988	0.94
191_D	246_Y	0.987	0.94
94_L	134_V	0.986	0.94
94_L	98_I	0.986	0.94
49_S	153_I	0.976	0.94
126_F	165_V	0.974	0.94
53_F	135_T	0.969	0.93
20_L	124_Y	0.968	0.93
76_D	82_Y	0.965	0.93
204_E	208_K	0.965	0.93
248_T	254_S	0.960	0.93
91_D	98_I	0.954	0.93
147_Y	151_E	0.954	0.93
224_G	227_N	0.952	0.93
7_T	163_K	0.952	0.93
18_T	81_T	0.952	0.93
77_F	82_Y	0.951	0.93
178_L	186_K	0.945	0.93
47_I	164_A	0.942	0.92
243_I	256_L	0.941	0.92
93_E	96_E	0.938	0.92
192_A	196_D	0.937	0.92
43_P	126_F	0.933	0.92
12_I	121_P	0.932	0.92
62_I	136_I	0.925	0.92
96_E	99_V	0.922	0.92
75_Y	79_D	0.912	0.91
27_K	31_R	0.911	0.91
184_T	252_G	0.908	0.91
193_I	212_L	0.907	0.91
46_I	86_Y	0.906	0.91
81_T	119_W	0.904	0.91
16_S	19_I	0.900	0.91
7_T	38_E	0.899	0.91
49_S	136_I	0.899	0.91
13_S	220_A	0.898	0.91
102_A	152_K	0.896	0.90
148_E	152_K	0.895	0.90
12_I	30_L	0.892	0.90
99_V	103_E	0.879	0.90
25_K	247_D	0.877	0.90
38_E	126_F	0.874	0.89
126_F	130_K	0.871	0.89
43_P	131_P	0.868	0.89
20_L	120_I	0.867	0.89
64_S	99_V	0.866	0.89
198_I	241_A	0.863	0.89
151_E	154_R	0.855	0.88
34_G	37_I	0.847	0.88
27_K	124_Y	0.829	0.87
60_F	136_I	0.821	0.86
70_C	85_C	0.814	0.86
144_E	207_L	0.806	0.85
30_L	124_Y	0.804	0.85
21_I	81_T	0.800	0.85
55_S	61_Y	0.798	0.85
203_F	207_L	0.796	0.84
105_K	109_D	0.782	0.83
186_K	218_Q	0.780	0.83
172_P	256_L	0.777	0.83
42_K	131_P	0.777	0.83
145_S	148_E	0.777	0.83
9_G	157_V	0.776	0.83
54_V	138_I	0.776	0.83
117_G	135_T	0.772	0.83
78_P	82_Y	0.768	0.82
254_S	257_A	0.767	0.82
203_F	233_L	0.766	0.82
79_D	82_Y	0.765	0.82
248_T	256_L	0.762	0.82
46_I	165_V	0.762	0.82
195_M	246_Y	0.757	0.81
50_S	118_A	0.756	0.81
196_D	200_S	0.754	0.81
74_Y	81_T	0.750	0.81
21_I	80_E	0.749	0.81
102_A	149_I	0.749	0.81
34_G	38_E	0.748	0.81
6_M	239_K	0.743	0.80
244_L	247_D	0.739	0.80
223_E	227_N	0.738	0.80
29_L	32_R	0.732	0.79
64_S	109_D	0.726	0.79
58_E	141_N	0.724	0.78
29_L	257_A	0.722	0.78
120_I	123_F	0.721	0.78
96_E	108_K	0.718	0.78
14_H	17_P	0.716	0.78
88_A	114_L	0.714	0.77
55_S	59_T	0.711	0.77
38_E	43_P	0.709	0.77
10_L	36_E	0.707	0.77
67_K	87_N	0.706	0.77
237_K	241_A	0.705	0.77
194_L	229_L	0.705	0.77
69_E	111_K	0.702	0.76
75_Y	78_P	0.702	0.76
142_S	146_H	0.698	0.76
189_K	192_A	0.697	0.76
50_S	63_E	0.696	0.76
18_T	21_I	0.694	0.75
23_E	27_K	0.694	0.75
157_V	163_K	0.693	0.75
244_L	256_L	0.693	0.75
195_M	243_I	0.692	0.75
65_S	114_L	0.690	0.75
31_R	35_K	0.689	0.75
190_F	193_I	0.685	0.74
28_D	31_R	0.685	0.74
241_A	244_L	0.684	0.74
155_K	158_E	0.681	0.74
210_S	223_E	0.680	0.74
58_E	140_D	0.679	0.74
68_L	87_N	0.672	0.73
18_T	22_D	0.672	0.73
104_G	152_K	0.671	0.73
143_P	223_E	0.668	0.73
75_Y	82_Y	0.667	0.72
207_L	230_H	0.666	0.72
63_E	114_L	0.666	0.72
128_E	148_E	0.666	0.72
98_I	136_I	0.665	0.72
143_P	226_L	0.665	0.72
13_S	19_I	0.664	0.72
10_L	125_M	0.663	0.72
209_I	217_Y	0.659	0.72
186_K	219_T	0.659	0.72
173_T	220_A	0.658	0.71
247_D	250_W	0.657	0.71
101_N	159_K	0.655	0.71
10_L	33_V	0.655	0.71
47_I	156_A	0.652	0.71
20_L	27_K	0.651	0.71
187_P	248_T	0.650	0.71
31_R	124_Y	0.648	0.70
173_T	219_T	0.648	0.70
26_W	30_L	0.647	0.70
25_K	29_L	0.647	0.70
74_Y	173_T	0.645	0.70
47_I	163_K	0.645	0.70
20_L	81_T	0.642	0.70
17_P	77_F	0.642	0.70
11_F	15_G	0.641	0.70
59_T	107_V	0.640	0.69
20_L	84_Y	0.639	0.69
46_I	172_P	0.639	0.69
63_E	70_C	0.638	0.69
96_E	151_E	0.635	0.69
63_E	134_V	0.634	0.69
187_P	219_T	0.634	0.69
19_I	120_I	0.631	0.68
146_H	227_N	0.630	0.68
199_K	242_E	0.629	0.68
68_L	114_L	0.629	0.68
193_I	214_P	0.627	0.68
88_A	129_Y	0.624	0.67
185_P	188_T	0.623	0.67
173_T	176_L	0.622	0.67
241_A	245_G	0.618	0.67
53_F	112_W	0.618	0.67
147_Y	230_H	0.616	0.66
212_L	216_E	0.616	0.66
51_P	169_T	0.616	0.66
119_W	173_T	0.612	0.66
70_C	135_T	0.609	0.66
70_C	87_N	0.608	0.65
67_K	89_L	0.607	0.65
205_K	208_K	0.607	0.65
54_V	223_E	0.606	0.65
18_T	78_P	0.604	0.65
26_W	29_L	0.603	0.65
14_H	169_T	0.599	0.64
192_A	195_M	0.596	0.64
173_T	186_K	0.596	0.64
22_D	80_E	0.593	0.64
230_H	233_L	0.592	0.63
154_R	238_P	0.592	0.63
143_P	227_N	0.592	0.63
43_P	46_I	0.591	0.63
73_D	77_F	0.591	0.63
196_D	199_K	0.589	0.63
86_Y	127_P	0.589	0.63
66_E	92_T	0.588	0.63
19_I	171_S	0.587	0.63
51_P	54_V	0.584	0.62
85_C	123_F	0.582	0.62
209_I	226_L	0.582	0.62
71_I	119_W	0.580	0.62
55_S	137_S	0.579	0.62
195_M	256_L	0.579	0.62
70_C	119_W	0.578	0.62
75_Y	85_C	0.576	0.61
166_F	244_L	0.574	0.61
53_F	138_I	0.572	0.61
139_T	149_I	0.571	0.61
64_S	134_V	0.571	0.61
12_I	125_M	0.569	0.60
44_E	91_D	0.569	0.60
6_M	237_K	0.566	0.60
135_T	169_T	0.565	0.60
13_S	173_T	0.561	0.59
72_Q	119_W	0.559	0.59
179_F	250_W	0.558	0.59
252_G	256_L	0.558	0.59
60_F	146_H	0.558	0.59
121_P	124_Y	0.556	0.59
133_V	165_V	0.556	0.59
32_R	36_E	0.556	0.59
220_A	224_G	0.554	0.58
34_G	126_F	0.553	0.58
172_P	254_S	0.553	0.58
198_I	229_L	0.549	0.58
209_I	223_E	0.548	0.58
206_I	233_L	0.548	0.58
105_K	108_K	0.546	0.57
73_D	76_D	0.543	0.57
98_I	106_V	0.543	0.57
196_D	205_K	0.542	0.57
11_F	14_H	0.541	0.57
68_L	88_A	0.540	0.56
229_L	258_A	0.535	0.56
46_I	125_M	0.535	0.56
128_E	211_E	0.534	0.56
201_G	236_I	0.531	0.55
244_L	257_A	0.529	0.55
193_I	232_L	0.527	0.55
172_P	220_A	0.527	0.55
222_P	255_M	0.526	0.54
241_A	246_Y	0.525	0.54
74_Y	77_F	0.524	0.54
58_E	75_Y	0.523	0.54
215_K	218_Q	0.523	0.54
187_P	252_G	0.521	0.54
68_L	90_N	0.520	0.54
16_S	75_Y	0.519	0.54
201_G	241_A	0.519	0.54
68_L	146_H	0.517	0.53
73_D	115_D	0.516	0.53
81_T	120_I	0.514	0.53
49_S	138_I	0.513	0.53
217_Y	223_E	0.511	0.52
190_F	216_E	0.510	0.52
52_H	174_H	0.510	0.52
45_T	156_A	0.507	0.52
10_L	232_L	0.507	0.52
100_K	128_E	0.506	0.52
204_E	207_L	0.506	0.52
28_D	32_R	0.505	0.52
55_S	139_T	0.505	0.52
152_K	155_K	0.505	0.52
72_Q	82_Y	0.502	0.51
101_N	164_A	0.501	0.51
156_A	164_A	0.501	0.51
136_I	231_M	0.500	0.51

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2pw6A	2	0.8893	100	0.276	Contact Map
1b4uB	2	0.9313	100	0.54	Contact Map
3wraA	2	0.9198	100	0.545	Contact Map
3vsjA	1	0.9542	100	0.559	Contact Map
3vsjB	2	0.958	100	0.587	Contact Map
3bczA	1	0.8855	100	0.656	Contact Map
4twbA	2	0.4733	62.6	0.959	Contact Map
3dahA	5	0.4847	62.5	0.959	Contact Map
1dkuA	5	0.4771	61	0.96	Contact Map
3lrtA	2	0.3702	56.3	0.961	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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