Q97WP7 - SULSO - GREMLIN Contacts

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Q97WP7 - Uncharacterized protein

UniProt:	Q97WP7	Sequences: MVKHHCRMVFKFKLGFFTDLHGSEYSFKRSLNIAKSKRVDFLIISGGLIAKEILFVEDIGGRYFLNGKKVNLNNLNEDALRSGKYIVVDKKEVIEDIKSDKSKLEKIVIEKASEQMSRWVKIAEEIFRLEKVFWSPAHGDIPQLDSILKNYGSNLINENVINLEEIQIVSLGFGSPIGNSYREIPDSELYIKGKSLLKDADKERLIMNFHMPPLNTKLDNAKVGLRKSHVGSKAVLDLITEFQPIISLHGHVHESTSMDKVGETIAINPGSLYQLGDPSIVTFEVHKELKSFGSVNVSKYVIKNIEFVSTNPLDVI	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	316 (245)
Sequences:	206 (113)
Seq/√Len:	7.2

SULSO - Q97WP7 Q97VA5 Q97WI0 Q97VA9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
180_S	183_E	5.611	1.00
135_S	156_I	4.813	0.99
243_Q	263_E	4.226	0.97
182_R	185_P	4.008	0.96
243_Q	262_G	3.274	0.89
170_S	208_N	3.160	0.87
180_S	185_P	3.055	0.86
182_R	186_D	3.047	0.85
238_L	242_F	2.510	0.72
183_E	186_D	2.508	0.72
174_G	184_I	2.381	0.67
180_S	186_D	2.303	0.65
184_I	192_K	2.245	0.63
185_P	188_E	2.168	0.60
135_S	155_L	2.149	0.59
194_K	241_E	2.139	0.59
236_L	240_T	2.136	0.59
190_Y	238_L	2.092	0.57
20_L	26_S	2.074	0.56
163_L	168_I	2.060	0.56
193_G	238_L	2.014	0.54
196_L	211_M	1.981	0.53
99_S	263_E	1.861	0.48
174_G	211_M	1.846	0.47
187_S	191_I	1.838	0.47
153_S	273_Y	1.816	0.46
179_N	183_E	1.803	0.46
109_I	143_Q	1.799	0.45
86_I	267_I	1.721	0.42
134_W	149_K	1.710	0.42
115_Q	120_V	1.697	0.42
94_I	257_S	1.694	0.41
162_N	165_E	1.681	0.41
61_G	90_K	1.677	0.41
178_G	182_R	1.675	0.41
132_V	152_G	1.665	0.40
77_E	195_S	1.657	0.40
47_G	137_A	1.653	0.40
61_G	114_E	1.631	0.39
30_S	123_A	1.609	0.38
163_L	188_E	1.585	0.37
177_I	230_V	1.567	0.37
20_L	40_D	1.564	0.37
113_S	117_S	1.536	0.36
106_K	237_D	1.510	0.35
13_K	92_E	1.507	0.35
178_G	183_E	1.468	0.33
175_S	211_M	1.465	0.33
49_I	85_Y	1.458	0.33
177_I	183_E	1.453	0.33
171_L	209_F	1.453	0.33
79_A	134_W	1.428	0.32
229_H	266_A	1.414	0.31
139_G	271_S	1.410	0.31
177_I	180_S	1.383	0.30
209_F	235_V	1.362	0.30
80_L	197_L	1.341	0.29
108_V	123_A	1.337	0.29
97_I	165_E	1.337	0.29
133_F	163_L	1.317	0.28
102_S	112_A	1.302	0.28
17_F	39_V	1.293	0.27
44_I	136_P	1.286	0.27
17_F	30_S	1.283	0.27
232_S	276_G	1.282	0.27
188_E	191_I	1.278	0.27
113_S	123_A	1.277	0.27
85_Y	186_D	1.273	0.27
94_I	247_S	1.269	0.27
54_L	66_N	1.259	0.26
20_L	48_L	1.255	0.26
43_I	49_I	1.251	0.26
131_K	144_L	1.251	0.26
106_K	186_D	1.251	0.26
186_D	230_V	1.249	0.26
112_A	124_E	1.247	0.26
45_S	116_M	1.219	0.25
18_T	163_L	1.218	0.25
103_K	260_K	1.210	0.25
118_R	277_D	1.207	0.25
131_K	164_E	1.202	0.25
180_S	230_V	1.194	0.25
235_V	275_L	1.186	0.24
171_L	247_S	1.181	0.24
77_E	248_L	1.177	0.24
101_K	125_E	1.176	0.24
87_V	267_I	1.176	0.24
43_I	103_K	1.168	0.24
180_S	184_I	1.163	0.24
69_K	107_I	1.160	0.24
193_G	196_L	1.158	0.24
54_L	78_D	1.158	0.24
58_D	80_L	1.154	0.23
47_G	138_H	1.151	0.23
164_E	206_I	1.143	0.23
20_L	79_A	1.141	0.23
82_S	97_I	1.129	0.23
118_R	238_L	1.128	0.23
232_S	251_H	1.124	0.23
174_G	257_S	1.123	0.23
42_L	265_I	1.119	0.22
181_Y	185_P	1.111	0.22
85_Y	276_G	1.105	0.22
43_I	243_Q	1.084	0.21
93_V	177_I	1.077	0.21
77_E	233_K	1.072	0.21
121_K	188_E	1.068	0.21
23_S	93_V	1.061	0.21
43_I	80_L	1.059	0.21
213_P	270_G	1.042	0.20
116_M	158_E	1.039	0.20
108_V	252_V	1.030	0.20
61_G	160_V	1.029	0.20
219_D	250_G	1.024	0.20
212_P	219_D	1.024	0.20
210_H	250_G	1.024	0.20
212_P	250_G	1.024	0.20
19_D	46_G	1.024	0.20
210_H	219_D	1.024	0.20
46_G	210_H	1.024	0.20
46_G	212_P	1.024	0.20
46_G	219_D	1.024	0.20
46_G	250_G	1.024	0.20
19_D	250_G	1.024	0.20
19_D	219_D	1.024	0.20
19_D	212_P	1.024	0.20
19_D	210_H	1.024	0.20
260_K	265_I	1.006	0.20
57_E	273_Y	1.000	0.19
195_S	234_A	0.988	0.19
13_K	39_V	0.982	0.19
31_L	269_P	0.979	0.19
163_L	266_A	0.977	0.19
258_M	267_I	0.976	0.19
107_I	233_K	0.975	0.19
234_A	275_L	0.973	0.19
141_I	175_S	0.972	0.19
68_K	114_E	0.969	0.19
90_K	247_S	0.968	0.19
73_N	189_L	0.964	0.19
155_L	206_I	0.961	0.18
49_I	89_D	0.960	0.18
59_I	133_F	0.949	0.18
178_G	186_D	0.949	0.18
162_N	191_I	0.949	0.18
154_N	258_M	0.942	0.18
60_G	153_S	0.940	0.18
168_I	206_I	0.938	0.18
243_Q	264_T	0.932	0.18
114_E	144_L	0.931	0.18
57_E	75_L	0.931	0.18
61_G	187_S	0.928	0.18
237_D	259_D	0.923	0.18
181_Y	238_L	0.921	0.18
189_L	234_A	0.911	0.17
69_K	94_I	0.910	0.17
118_R	162_N	0.904	0.17
84_K	267_I	0.898	0.17
59_I	231_G	0.898	0.17
81_R	165_E	0.897	0.17
181_Y	186_D	0.892	0.17
25_Y	122_I	0.887	0.17
67_G	75_L	0.882	0.17
13_K	18_T	0.878	0.17
237_D	240_T	0.869	0.16
177_I	276_G	0.865	0.16
107_I	134_W	0.864	0.16
177_I	240_T	0.863	0.16
53_I	57_E	0.863	0.16
39_V	170_S	0.857	0.16
181_Y	230_V	0.856	0.16
85_Y	160_V	0.846	0.16
174_G	230_V	0.839	0.16
156_I	160_V	0.837	0.16
80_L	238_L	0.831	0.16
116_M	122_I	0.823	0.15
161_I	242_F	0.822	0.15
173_F	272_L	0.820	0.15
80_L	106_K	0.816	0.15
14_L	39_V	0.811	0.15
39_V	136_P	0.807	0.15
38_R	142_P	0.801	0.15
123_A	276_G	0.799	0.15
96_D	190_Y	0.797	0.15
188_E	192_K	0.796	0.15
178_G	181_Y	0.794	0.15
85_Y	88_V	0.790	0.15
166_I	267_I	0.789	0.15
76_N	111_K	0.786	0.15
84_K	258_M	0.784	0.15
54_L	107_I	0.782	0.15
74_N	93_V	0.781	0.15
73_N	96_D	0.781	0.15
43_I	265_I	0.781	0.15
77_E	276_G	0.780	0.15
108_V	125_E	0.778	0.15
247_S	266_A	0.777	0.15
21_H	140_D	0.776	0.14
248_L	277_D	0.774	0.14
40_D	215_N	0.770	0.14
88_V	258_M	0.768	0.14
91_K	233_K	0.768	0.14
118_R	133_F	0.767	0.14
22_G	270_G	0.758	0.14
69_K	211_M	0.754	0.14
171_L	218_L	0.751	0.14
116_M	120_V	0.745	0.14
215_N	238_L	0.745	0.14
22_G	251_H	0.744	0.14
75_L	79_A	0.744	0.14
57_E	94_I	0.742	0.14
179_N	185_P	0.741	0.14
252_V	279_S	0.741	0.14
133_F	168_I	0.740	0.14
181_Y	193_G	0.739	0.14
174_G	183_E	0.736	0.14
58_D	158_E	0.735	0.14
83_G	273_Y	0.734	0.14
43_I	154_N	0.734	0.14
66_N	179_N	0.733	0.14
106_K	188_E	0.729	0.14
24_E	263_E	0.727	0.14
178_G	273_Y	0.724	0.14
190_Y	267_I	0.721	0.13
66_N	78_D	0.717	0.13
177_I	186_D	0.711	0.13
47_G	250_G	0.710	0.13
47_G	212_P	0.710	0.13
47_G	219_D	0.710	0.13
47_G	210_H	0.710	0.13
19_D	47_G	0.710	0.13
19_D	253_H	0.709	0.13
46_G	253_H	0.709	0.13
210_H	253_H	0.709	0.13
219_D	253_H	0.709	0.13
212_P	253_H	0.709	0.13
250_G	253_H	0.709	0.13
239_I	242_F	0.709	0.13
179_N	186_D	0.703	0.13
177_I	211_M	0.700	0.13
42_L	213_P	0.699	0.13
159_N	191_I	0.699	0.13
146_S	194_K	0.697	0.13
139_G	252_V	0.689	0.13
149_K	196_L	0.688	0.13
61_G	189_L	0.688	0.13
105_E	112_A	0.684	0.13
99_S	176_P	0.683	0.13
101_K	231_G	0.683	0.13
115_Q	122_I	0.681	0.13
25_Y	110_E	0.677	0.13
87_V	90_K	0.675	0.13
41_F	144_L	0.673	0.13
155_L	259_D	0.669	0.13
26_S	151_Y	0.667	0.13
14_L	72_L	0.667	0.13
198_K	259_D	0.666	0.12
183_E	230_V	0.664	0.12
100_D	252_V	0.662	0.12
42_L	79_A	0.662	0.12
106_K	109_I	0.660	0.12
77_E	162_N	0.660	0.12
179_N	182_R	0.659	0.12
197_L	238_L	0.657	0.12
121_K	234_A	0.652	0.12
58_D	133_F	0.651	0.12
16_F	42_L	0.645	0.12
28_K	61_G	0.637	0.12
173_F	252_V	0.636	0.12
21_H	219_D	0.634	0.12
21_H	212_P	0.634	0.12
21_H	210_H	0.634	0.12
21_H	46_G	0.634	0.12
21_H	250_G	0.634	0.12
146_S	261_V	0.634	0.12
95_E	105_E	0.633	0.12
67_G	79_A	0.632	0.12
19_D	268_N	0.627	0.12
250_G	268_N	0.627	0.12
46_G	268_N	0.627	0.12
219_D	268_N	0.627	0.12
212_P	268_N	0.627	0.12
210_H	268_N	0.627	0.12
140_D	270_G	0.625	0.12
96_D	185_P	0.624	0.12
97_I	234_A	0.620	0.12
137_A	212_P	0.618	0.12
46_G	137_A	0.618	0.12
137_A	210_H	0.618	0.12
137_A	250_G	0.618	0.12
137_A	219_D	0.618	0.12
19_D	137_A	0.618	0.12
163_L	237_D	0.617	0.12
45_S	48_L	0.616	0.12
55_F	272_L	0.615	0.12
69_K	84_K	0.612	0.12
114_E	154_N	0.611	0.12
228_S	267_I	0.606	0.12
208_N	255_S	0.605	0.11
76_N	88_V	0.605	0.11
93_V	242_F	0.603	0.11
199_D	237_D	0.602	0.11
79_A	82_S	0.600	0.11
233_K	261_V	0.596	0.11
18_T	116_M	0.596	0.11
142_P	206_I	0.595	0.11
235_V	261_V	0.594	0.11
229_H	259_D	0.594	0.11
86_I	174_G	0.594	0.11
58_D	131_K	0.593	0.11
20_L	111_K	0.592	0.11
41_F	142_P	0.589	0.11
197_L	236_L	0.587	0.11
154_N	189_L	0.586	0.11
22_G	232_S	0.584	0.11
85_Y	267_I	0.582	0.11
85_Y	107_I	0.579	0.11
192_K	274_Q	0.578	0.11
68_K	153_S	0.578	0.11
119_W	208_N	0.577	0.11
49_I	136_P	0.577	0.11
188_E	194_K	0.576	0.11
154_N	159_N	0.575	0.11
42_L	260_K	0.574	0.11
183_E	257_S	0.573	0.11
99_S	234_A	0.573	0.11
193_G	229_H	0.572	0.11
136_P	157_N	0.567	0.11
18_T	135_S	0.564	0.11
262_G	265_I	0.560	0.11
102_S	196_L	0.557	0.11
99_S	206_I	0.557	0.11
187_S	236_L	0.556	0.11
92_E	113_S	0.555	0.11
109_I	277_D	0.554	0.11
31_L	52_E	0.549	0.11
48_L	61_G	0.548	0.11
70_V	82_S	0.548	0.11
111_K	163_L	0.545	0.11
176_P	183_E	0.544	0.11
54_L	192_K	0.543	0.11
191_I	195_S	0.542	0.11
260_K	269_P	0.537	0.10
102_S	247_S	0.537	0.10
92_E	99_S	0.535	0.10
49_I	145_D	0.533	0.10
155_L	168_I	0.532	0.10
87_V	197_L	0.530	0.10
38_R	214_L	0.526	0.10
198_K	241_E	0.524	0.10
52_E	136_P	0.522	0.10
120_V	124_E	0.521	0.10
152_G	194_K	0.521	0.10
131_K	142_P	0.521	0.10
49_I	121_K	0.520	0.10
124_E	192_K	0.519	0.10
24_E	216_T	0.518	0.10
66_N	112_A	0.518	0.10
101_K	206_I	0.516	0.10
133_F	231_G	0.516	0.10
49_I	258_M	0.515	0.10
14_L	159_N	0.513	0.10
60_G	141_I	0.510	0.10
40_D	131_K	0.505	0.10
96_D	233_K	0.505	0.10
17_F	27_F	0.502	0.10
239_I	270_G	0.502	0.10
77_E	181_Y	0.500	0.10

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2yvtA	1	0.769	100	0.755	Contact Map
1uf3A	6	0.6677	100	0.758	Contact Map
4peiA	2	0.7911	99.9	0.784	Contact Map
3rl5A	1	0.6646	99.9	0.789	Contact Map
3qfmA	2	0.7057	99.9	0.811	Contact Map
3ib7A	2	0.788	99.9	0.815	Contact Map
3d03A	5	0.7468	99.9	0.815	Contact Map
3rqzA	1	0.6772	99.9	0.819	Contact Map
4ykeA	2	0.8481	99.8	0.821	Contact Map
1nnwA	2	0.7152	99.8	0.824	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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