Q97WP5 - SULSO - GREMLIN Contacts

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Q97WP5 - Uncharacterized protein

UniProt:	Q97WP5	Sequences: MIEEERNNYSVFHGLWTLASRELKKWYKAPVILLLSLIQPIFWIGIFGKAMNLGSIFTETTFNIPGLTIPKQVLDQIGLEILKQTFGTTDYFSFLAAGMLSFIVLFTSMQSGMSIVWDRRLGVLDRILTTPVPRGNIIIGKVLSAVIRSLVQATIVLVVAVLLGMTFAPEINPLDFLGVYAALFLMSFGLSSLFLMLALRATSWESQMAIMNLLNLPLLFTSNAFYPVKSMPYWLKPVAYINPLTYSNGVARGMLLGISTNLAIDFLYLGLFAIILSTIGIVLSWKYLSK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	290 (238)
Sequences:	22933 (15886)
Seq/√Len:	1029.7

ECOLI - YADH YHHJ YBHS YBHR
BACSU - YVFS YFIM YFIN NATB TAGG YHAP
HALSA - Q9HRJ5 Q9HMU9
SULSO - Q97WP5 Q97VH7 Q7LY08 Q97U55 Q97Z04 Q97W41

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
182_A	250_V	5.131	1.00
20_S	24_K	3.795	1.00
14_G	136_N	3.120	1.00
196_M	279_I	3.116	1.00
19_A	147_I	3.025	1.00
186_M	247_S	3.013	1.00
18_L	140_G	2.983	1.00
15_L	143_L	2.904	1.00
16_W	20_S	2.750	1.00
22_E	141_K	2.704	1.00
154_T	180_Y	2.581	1.00
133_P	136_N	2.529	1.00
233_Y	237_P	2.515	1.00
19_A	143_L	2.471	1.00
185_L	270_G	2.354	1.00
189_G	273_A	2.324	1.00
188_F	273_A	2.310	1.00
263_A	267_L	2.164	1.00
24_K	27_Y	2.125	1.00
90_D	93_S	2.121	1.00
264_I	268_Y	1.967	1.00
15_L	19_A	1.945	1.00
15_L	136_N	1.881	1.00
27_Y	30_P	1.875	1.00
189_G	276_L	1.859	1.00
267_L	271_L	1.804	1.00
278_T	282_V	1.777	1.00
178_G	254_M	1.776	1.00
268_Y	271_L	1.747	1.00
118_D	141_K	1.736	1.00
233_Y	236_K	1.736	1.00
203_S	206_S	1.714	1.00
239_A	245_T	1.703	1.00
236_K	240_Y	1.693	1.00
234_W	237_P	1.649	1.00
192_S	277_S	1.639	1.00
192_S	273_A	1.634	1.00
272_F	276_L	1.610	1.00
201_A	210_I	1.594	1.00
268_Y	272_F	1.591	1.00
228_V	239_A	1.591	1.00
246_Y	265_D	1.588	1.00
196_M	276_L	1.554	1.00
150_L	184_F	1.536	1.00
264_I	267_L	1.517	1.00
196_M	283_L	1.511	1.00
182_A	266_F	1.509	1.00
186_M	190_L	1.470	1.00
275_I	279_I	1.468	1.00
15_L	139_I	1.428	1.00
178_G	262_L	1.381	1.00
240_Y	246_Y	1.377	1.00
182_A	254_M	1.367	1.00
183_L	247_S	1.360	1.00
158_V	161_V	1.360	1.00
15_L	140_G	1.357	1.00
146_V	184_F	1.357	1.00
249_G	265_D	1.356	1.00
185_L	273_A	1.348	1.00
159_V	162_L	1.347	1.00
91_Y	94_F	1.346	1.00
177_L	181_A	1.338	1.00
122_G	125_D	1.327	1.00
140_G	144_S	1.326	1.00
273_A	277_S	1.325	1.00
237_P	241_I	1.323	1.00
49_K	52_N	1.321	1.00
266_F	270_G	1.312	1.00
21_R	118_D	1.310	1.00
271_L	275_I	1.300	1.00
197_L	201_A	1.295	1.00
123_V	126_R	1.290	1.00
234_W	238_V	1.284	1.00
149_S	187_S	1.282	1.00
16_W	19_A	1.263	1.00
185_L	266_F	1.262	1.00
199_L	283_L	1.261	1.00
112_G	207_Q	1.252	1.00
237_P	240_Y	1.242	1.00
96_A	99_M	1.237	1.00
189_G	272_F	1.236	1.00
212_N	216_L	1.228	1.00
112_G	115_I	1.227	1.00
249_G	253_G	1.214	1.00
231_M	236_K	1.208	1.00
270_G	274_I	1.198	1.00
120_R	204_W	1.188	1.00
146_V	188_F	1.185	1.00
19_A	140_G	1.182	1.00
52_N	55_S	1.180	1.00
23_L	27_Y	1.159	1.00
178_G	181_A	1.155	1.00
192_S	276_L	1.144	1.00
261_N	264_I	1.142	1.00
189_G	269_L	1.136	1.00
240_Y	245_T	1.132	1.00
246_Y	268_Y	1.132	1.00
23_L	144_S	1.131	1.00
123_V	127_I	1.125	1.00
279_I	283_L	1.122	1.00
24_K	28_K	1.121	1.00
135_G	139_I	1.121	1.00
19_A	144_S	1.115	1.00
110_Q	113_M	1.114	1.00
17_T	20_S	1.110	1.00
157_L	179_V	1.110	1.00
34_L	38_I	1.095	1.00
224_A	248_N	1.094	1.00
127_I	132_V	1.076	1.00
213_L	217_P	1.074	1.00
249_G	261_N	1.072	1.00
275_I	278_T	1.072	1.00
14_G	133_P	1.063	1.00
19_A	23_L	1.051	1.00
40_P	46_I	1.046	1.00
201_A	206_S	1.044	1.00
218_L	242_N	1.039	1.00
13_H	16_W	1.037	1.00
260_T	263_A	1.035	1.00
192_S	280_G	1.029	1.00
111_S	115_I	1.020	1.00
200_R	283_L	1.017	1.00
22_E	144_S	1.007	1.00
48_G	52_N	0.997	1.00
228_V	245_T	0.994	1.00
117_W	120_R	0.987	1.00
97_A	100_L	0.984	1.00
246_Y	250_V	0.978	1.00
196_M	280_G	0.961	1.00
127_I	131_P	0.950	1.00
202_T	206_S	0.942	1.00
100_L	103_I	0.940	1.00
177_L	180_Y	0.933	1.00
39_Q	43_W	0.931	1.00
111_S	114_S	0.930	1.00
141_K	145_A	0.925	1.00
138_I	280_G	0.924	1.00
124_L	127_I	0.923	1.00
263_A	266_F	0.923	1.00
138_I	281_I	0.923	1.00
236_K	239_A	0.922	1.00
242_N	245_T	0.921	1.00
97_A	101_S	0.920	1.00
23_L	147_I	0.919	1.00
187_S	191_S	0.918	1.00
90_D	94_F	0.915	1.00
193_L	214_L	0.904	1.00
166_T	175_D	0.902	1.00
193_L	276_L	0.899	1.00
246_Y	272_F	0.893	1.00
174_L	177_L	0.878	1.00
181_A	185_L	0.871	1.00
181_A	266_F	0.870	1.00
261_N	265_D	0.868	1.00
245_T	249_G	0.859	1.00
40_P	43_W	0.859	1.00
105_L	108_S	0.859	1.00
99_M	103_I	0.858	1.00
232_P	236_K	0.857	1.00
176_F	180_Y	0.857	1.00
175_D	178_G	0.854	1.00
185_L	269_L	0.853	1.00
13_H	17_T	0.853	1.00
116_V	207_Q	0.848	1.00
121_L	125_D	0.846	1.00
262_L	265_D	0.845	1.00
22_E	145_A	0.844	1.00
265_D	269_L	0.844	1.00
228_V	236_K	0.839	1.00
48_G	163_L	0.833	1.00
116_V	120_R	0.830	1.00
30_P	33_L	0.829	1.00
136_N	140_G	0.823	1.00
126_R	131_P	0.822	1.00
164_G	167_F	0.817	1.00
212_N	217_P	0.816	1.00
126_R	132_V	0.815	1.00
276_L	280_G	0.814	1.00
279_I	282_V	0.814	1.00
135_G	281_I	0.814	1.00
270_G	273_A	0.811	1.00
41_I	159_V	0.810	1.00
110_Q	115_I	0.810	1.00
228_V	240_Y	0.809	1.00
98_G	101_S	0.808	1.00
45_G	49_K	0.805	1.00
267_L	270_G	0.803	1.00
183_L	251_A	0.802	1.00
132_V	137_I	0.802	1.00
246_Y	249_G	0.801	1.00
150_L	154_T	0.800	1.00
99_M	102_F	0.800	1.00
180_Y	184_F	0.799	1.00
265_D	268_Y	0.797	1.00
274_I	278_T	0.794	1.00
253_G	258_I	0.791	1.00
93_S	96_A	0.790	1.00
269_L	272_F	0.788	1.00
26_W	36_S	0.787	1.00
250_V	265_D	0.782	1.00
124_L	137_I	0.778	1.00
95_L	99_M	0.778	1.00
97_A	102_F	0.778	1.00
116_V	119_R	0.774	1.00
41_I	45_G	0.772	1.00
142_V	192_S	0.771	1.00
254_M	262_L	0.770	1.00
22_E	25_K	0.764	1.00
262_L	266_F	0.762	1.00
29_A	32_I	0.753	1.00
101_S	104_V	0.751	1.00
208_M	212_N	0.751	1.00
274_I	277_S	0.750	1.00
157_L	183_L	0.747	1.00
128_L	134_R	0.746	1.00
48_G	162_L	0.739	1.00
21_R	25_K	0.739	1.00
153_A	183_L	0.738	1.00
95_L	98_G	0.738	1.00
87_G	90_D	0.737	1.00
154_T	158_V	0.735	1.00
264_I	271_L	0.734	1.00
160_A	179_V	0.733	1.00
118_D	123_V	0.732	1.00
243_P	247_S	0.724	1.00
211_M	215_N	0.721	1.00
206_S	209_A	0.713	1.00
155_I	158_V	0.712	1.00
209_A	212_N	0.711	1.00
158_V	176_F	0.707	1.00
122_G	132_V	0.704	1.00
139_I	143_L	0.703	1.00
142_V	277_S	0.701	1.00
97_A	108_S	0.700	1.00
183_L	187_S	0.699	1.00
91_Y	96_A	0.699	1.00
154_T	157_L	0.688	1.00
145_A	149_S	0.687	1.00
147_I	151_V	0.687	1.00
195_L	280_G	0.684	1.00
215_N	219_L	0.669	1.00
222_S	245_T	0.667	1.00
96_A	107_T	0.666	1.00
217_P	220_F	0.665	1.00
122_G	126_R	0.664	1.00
120_R	124_L	0.663	1.00
18_L	136_N	0.662	1.00
44_I	156_V	0.659	1.00
277_S	281_I	0.659	1.00
146_V	191_S	0.658	1.00
127_I	130_T	0.652	1.00
182_A	247_S	0.652	1.00
35_L	39_Q	0.652	1.00
26_W	148_R	0.651	1.00
247_S	269_L	0.649	1.00
223_N	227_P	0.649	1.00
110_Q	114_S	0.649	1.00
91_Y	95_L	0.648	1.00
195_L	199_L	0.642	1.00
23_L	151_V	0.642	1.00
179_V	251_A	0.641	1.00
214_L	218_L	0.633	1.00
44_I	160_A	0.625	1.00
45_G	159_V	0.625	1.00
231_M	235_L	0.623	1.00
257_G	260_T	0.620	1.00
15_L	135_G	0.620	1.00
241_I	246_Y	0.619	1.00
124_L	128_L	0.618	1.00
269_L	273_A	0.617	1.00
143_L	147_I	0.617	1.00
194_F	198_A	0.614	1.00
153_A	184_F	0.614	1.00
43_W	46_I	0.612	1.00
216_L	219_L	0.611	1.00
119_R	202_T	0.609	1.00
178_G	266_F	0.608	1.00
101_S	109_M	0.606	1.00
226_Y	230_S	0.604	1.00
248_N	252_R	0.602	1.00
100_L	108_S	0.601	1.00
174_L	178_G	0.599	1.00
253_G	261_N	0.599	1.00
31_V	35_L	0.597	1.00
27_Y	33_L	0.596	1.00
125_D	128_L	0.596	1.00
145_A	195_L	0.596	1.00
240_Y	249_G	0.594	1.00
43_W	47_F	0.593	1.00
98_G	107_T	0.589	1.00
193_L	196_M	0.589	1.00
105_L	190_L	0.587	1.00
195_L	198_A	0.585	1.00
142_V	191_S	0.583	1.00
98_G	102_F	0.580	1.00
239_A	242_N	0.580	1.00
117_W	121_L	0.579	1.00
212_N	215_N	0.579	1.00
278_T	281_I	0.579	1.00
98_G	103_I	0.579	1.00
96_A	100_L	0.577	1.00
199_L	280_G	0.570	1.00
26_W	144_S	0.569	1.00
126_R	130_T	0.566	1.00
49_K	162_L	0.564	1.00
17_T	24_K	0.563	1.00
210_I	214_L	0.562	1.00
253_G	265_D	0.557	1.00
272_F	275_I	0.557	1.00
89_T	93_S	0.554	1.00
197_L	214_L	0.553	1.00
219_L	222_S	0.552	1.00
196_M	199_L	0.550	1.00
92_F	96_A	0.550	1.00
161_V	167_F	0.549	1.00
147_I	150_L	0.544	1.00
105_L	109_M	0.543	1.00
146_V	150_L	0.540	1.00
112_G	211_M	0.540	1.00
188_F	192_S	0.539	1.00
122_G	131_P	0.539	1.00
116_V	204_W	0.539	1.00
197_L	211_M	0.538	1.00
227_P	230_S	0.537	1.00
51_M	56_I	0.537	1.00
108_S	194_F	0.534	1.00
36_S	148_R	0.533	1.00
230_S	233_Y	0.530	1.00
102_F	108_S	0.528	1.00
14_G	17_T	0.528	1.00
41_I	155_I	0.527	1.00
268_Y	276_L	0.526	1.00
252_R	256_L	0.526	1.00
178_G	182_A	0.524	1.00
92_F	97_A	0.522	1.00
125_D	131_P	0.521	1.00
85_T	88_T	0.521	1.00
101_S	244_L	0.519	1.00
106_F	109_M	0.519	1.00
110_Q	116_V	0.518	1.00
250_V	262_L	0.518	1.00
148_R	152_Q	0.518	1.00
197_L	200_R	0.516	1.00
38_I	41_I	0.513	1.00
22_E	137_I	0.513	1.00
84_Q	88_T	0.512	1.00
103_I	108_S	0.509	1.00
228_V	231_M	0.507	1.00
87_G	91_Y	0.506	1.00
281_I	284_S	0.505	1.00
151_V	154_T	0.503	1.00
276_L	279_I	0.501	1.00
128_L	131_P	0.501	1.00
47_F	52_N	0.501	1.00
112_G	118_D	0.501	1.00
21_R	126_R	0.500	1.00
101_S	248_N	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2rh1A	1	0.6172	10.5	0.941	Contact Map
2z73A	2	0.7069	9.7	0.942	Contact Map
2wswA	3	0.5448	7.7	0.944	Contact Map
4ib4A	1	0.6241	7.7	0.944	Contact Map
2rddB	1	0.1034	6.6	0.946	Contact Map
2ks9A	1	0.7034	5.9	0.947	Contact Map
3j9pD	4	0.7828	5.1	0.949	Contact Map
4grvA	1	0.6517	5	0.949	Contact Map
4hfiA	3	0.5069	4.8	0.949	Contact Map
2r4rA	1	0.4138	4.7	0.95	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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