GREMLIN Database
NADE - NH(3)-dependent NAD(+) synthetase
UniProt: Q97WN9 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 278 (246)
Sequences: 4355 (2503)
Seq/√Len: 159.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
184_E188_T4.7821.00
63_L130_Y3.6201.00
53_L139_V3.5431.00
35_G136_Y3.4251.00
245_R249_I2.9241.00
17_T179_C2.8651.00
80_E185_R2.6911.00
72_K76_D2.6361.00
62_I87_A2.5271.00
48_V177_G2.4471.00
242_K246_L2.3131.00
28_L36_G2.2881.00
24_I52_L2.2711.00
237_I241_K2.2501.00
14_E179_C2.2001.00
40_V82_I2.1071.00
77_D189_K2.0751.00
82_I91_Y2.0701.00
50_A87_A2.0101.00
49_T165_I1.9511.00
97_D101_K1.9441.00
79_F83_K1.9341.00
103_F125_I1.9271.00
39_G127_L1.8851.00
251_Q255_L1.8141.00
166_G259_I1.8131.00
122_V125_I1.7661.00
13_C175_M1.7571.00
98_E105_N1.7001.00
68_S97_D1.6981.00
17_T55_K1.6911.00
44_V177_G1.6761.00
80_E83_K1.6551.00
226_E241_K1.6411.00
218_L224_R1.6241.00
77_D185_R1.6201.00
175_M178_K1.6041.00
90_K134_L1.5931.00
103_F122_V1.5701.00
200_T203_G1.5591.00
178_K184_E1.5381.00
61_F92_K1.5311.00
64_L91_Y1.5121.00
47_A186_I1.5101.00
174_R210_E1.5041.00
118_I125_I1.4961.00
178_K187_V1.4291.00
152_F158_G1.4231.00
36_G53_L1.4101.00
22_E26_E1.4101.00
130_Y134_L1.4031.00
14_E18_N1.4011.00
61_F136_Y1.4011.00
31_S137_L1.3971.00
79_F93_L1.3951.00
211_T239_I1.3861.00
44_V173_V1.3781.00
35_G61_F1.3761.00
171_T214_S1.3661.00
52_L164_P1.3651.00
169_Y172_Q1.3531.00
208_D211_T1.3501.00
54_A57_T1.3391.00
80_E84_F1.3371.00
57_T60_F1.3371.00
218_L229_I1.3371.00
73_I185_R1.3301.00
114_I129_A1.3251.00
190_P200_T1.3231.00
99_I106_K1.3171.00
34_K135_D1.3121.00
35_G59_N1.3061.00
149_L212_I1.3041.00
202_E208_D1.2971.00
76_D185_R1.2911.00
160_V163_L1.2861.00
149_L205_L1.2831.00
81_M186_I1.2821.00
217_Y222_E1.2821.00
219_R244_D1.2701.00
36_G139_V1.2631.00
154_K252_H1.2621.00
51_T55_K1.2581.00
100_V122_V1.2521.00
246_L249_I1.2501.00
224_R228_E1.2461.00
216_L247_V1.2431.00
50_A85_L1.2431.00
64_L75_L1.2381.00
79_F91_Y1.2331.00
55_K180_L1.2181.00
218_L233_L1.2181.00
54_A85_L1.2151.00
18_N22_E1.2121.00
144_K167_D1.2051.00
24_I36_G1.1961.00
246_L250_S1.1911.00
50_A62_I1.1841.00
214_S235_I1.1771.00
27_Y30_F1.1711.00
158_G256_P1.1581.00
49_T139_V1.1571.00
171_T217_Y1.1541.00
75_L93_L1.1521.00
100_V104_S1.1511.00
250_S254_R1.1441.00
76_D80_E1.1431.00
37_V130_Y1.1431.00
64_L78_S1.1351.00
17_T180_L1.1351.00
75_L79_F1.1331.00
92_K134_L1.1181.00
169_Y216_L1.1171.00
178_K188_T1.1051.00
99_I126_I1.1031.00
17_T52_L1.0840.99
168_L172_Q1.0830.99
155_Y159_G1.0830.99
149_L246_L1.0750.99
23_R260_F1.0720.99
68_S100_V1.0700.99
242_K245_R1.0690.99
99_I103_F1.0670.99
196_W204_E1.0660.99
236_P239_I1.0600.99
29_E59_N1.0550.99
224_R233_L1.0510.99
44_V187_V1.0470.99
54_A60_F1.0440.99
99_I122_V1.0350.99
101_K104_S1.0350.99
140_G160_V1.0270.99
61_F90_K1.0270.99
115_I133_M1.0220.99
174_R190_P1.0220.99
74_D194_A1.0170.99
67_S100_V1.0150.99
62_I82_I1.0110.99
162_V260_F1.0090.99
193_P201_A1.0030.99
241_K245_R0.9930.99
193_P199_Q0.9910.99
142_G152_F0.9890.99
64_L79_F0.9840.99
139_V162_V0.9830.99
25_R29_E0.9830.99
28_L31_S0.9780.99
17_T21_V0.9780.99
211_T236_P0.9740.99
143_D201_A0.9730.99
99_I102_S0.9690.99
225_S228_E0.9660.99
73_I189_K0.9660.99
124_M159_G0.9650.99
79_F82_I0.9590.99
226_E230_V0.9580.99
238_D242_K0.9540.99
21_V56_A0.9510.99
251_Q254_R0.9500.99
94_I106_K0.9440.99
230_V240_V0.9380.98
21_V25_R0.9280.98
70_T73_I0.9180.98
24_I162_V0.9170.98
163_L166_G0.9160.98
144_K221_D0.9160.98
51_T85_L0.9050.98
230_V237_I0.9030.98
84_F182_L0.8990.98
20_I52_L0.8940.98
107_I129_A0.8930.98
114_I133_M0.8910.98
20_I167_D0.8900.98
62_I91_Y0.8890.98
218_L222_E0.8880.98
98_E106_K0.8850.98
172_Q175_M0.8740.98
199_Q204_E0.8730.98
38_I49_T0.8600.97
226_E244_D0.8540.97
177_G187_V0.8530.97
154_K161_D0.8380.97
101_K105_N0.8340.97
161_D252_H0.8300.97
94_I126_I0.8300.97
96_I122_V0.8300.97
34_K59_N0.8280.97
191_S201_A0.8250.97
147_L152_F0.8240.97
122_V126_I0.8220.96
118_I129_A0.8160.96
26_E30_F0.8150.96
39_G65_M0.8150.96
16_V172_Q0.8130.96
117_N153_T0.8070.96
76_D79_F0.8070.96
214_S218_L0.8020.96
155_Y160_V0.7970.96
62_I66_P0.7930.96
214_S219_R0.7900.96
102_S105_N0.7850.95
59_N90_K0.7800.95
138_V159_G0.7790.95
212_I243_V0.7790.95
128_Y161_D0.7780.95
81_M84_F0.7680.95
35_G134_L0.7670.95
61_F134_L0.7640.95
33_K135_D0.7630.95
47_A81_M0.7610.95
48_V173_V0.7530.94
95_N98_E0.7480.94
173_V177_G0.7460.94
21_V55_K0.7440.94
37_V136_Y0.7380.94
129_A133_M0.7380.94
67_S101_K0.7370.94
238_D241_K0.7340.93
219_R229_I0.7340.93
143_D191_S0.7320.93
169_Y217_Y0.7310.93
68_S101_K0.7290.93
116_G204_E0.7280.93
24_I164_P0.7270.93
230_V233_L0.7260.93
248_K251_Q0.7260.93
199_Q203_G0.7240.93
143_D193_P0.7210.93
28_L35_G0.7190.93
215_I240_V0.7160.93
151_Y204_E0.7160.93
22_E25_R0.7150.93
38_I50_A0.7140.92
117_N121_R0.7090.92
235_I240_V0.7030.92
174_R187_V0.7000.92
111_N133_M0.6970.92
53_L60_F0.6940.91
228_E231_K0.6930.91
102_S106_K0.6930.91
213_D216_L0.6920.91
54_A87_A0.6890.91
147_L166_G0.6880.91
151_Y193_P0.6870.91
119_K194_A0.6860.91
65_M138_V0.6840.91
67_S96_I0.6810.91
65_M126_I0.6790.91
200_T204_E0.6780.90
239_I242_K0.6750.90
73_I77_D0.6720.90
140_G159_G0.6700.90
227_D231_K0.6690.90
16_V20_I0.6680.90
13_C179_C0.6680.90
63_L126_I0.6670.90
51_T81_M0.6660.90
82_I87_A0.6650.90
131_A252_H0.6640.90
64_L70_T0.6610.89
51_T84_F0.6600.89
138_V252_H0.6550.89
114_I132_Q0.6510.89
116_G196_W0.6510.89
71_P194_A0.6500.89
240_V244_D0.6480.88
41_S45_D0.6450.88
171_T210_E0.6440.88
94_I107_I0.6400.88
80_E183_P0.6390.88
64_L93_L0.6320.87
171_T213_D0.6280.87
65_M127_L0.6260.87
112_K115_I0.6230.86
74_D189_K0.6180.86
62_I186_I0.6180.86
31_S166_G0.6170.86
96_I100_V0.6160.86
36_G137_L0.6150.86
211_T214_S0.6140.86
131_A136_Y0.6140.86
144_K166_G0.6130.85
109_T112_K0.6120.85
145_S212_I0.6120.85
87_A91_Y0.6100.85
227_D237_I0.6100.85
64_L67_S0.6100.85
12_D15_A0.6090.85
76_D83_K0.6080.85
36_G162_V0.6050.85
98_E101_K0.6040.85
113_Y116_G0.6040.85
175_M179_C0.6030.85
212_I215_I0.5990.84
98_E102_S0.5970.84
77_D80_E0.5960.84
73_I76_D0.5950.84
174_R178_K0.5950.84
26_E29_E0.5950.84
51_T182_L0.5940.84
151_Y199_Q0.5930.84
57_T86_N0.5910.83
92_K130_Y0.5890.83
147_L163_L0.5870.83
75_L78_S0.5860.83
202_E210_E0.5850.83
63_L92_K0.5850.83
148_L247_V0.5840.83
219_R248_K0.5840.83
214_S217_Y0.5820.82
90_K136_Y0.5810.82
120_A195_L0.5800.82
118_I122_V0.5780.82
104_S118_I0.5780.82
25_R56_A0.5780.82
142_G158_G0.5760.82
111_N114_I0.5760.82
51_T54_A0.5760.82
215_I244_D0.5740.82
30_F251_Q0.5690.81
65_M68_S0.5670.81
206_G209_Y0.5660.81
230_V234_G0.5650.81
36_G60_F0.5640.80
69_S111_N0.5600.80
48_V180_L0.5540.79
215_I229_I0.5530.79
223_M226_E0.5520.79
225_S229_I0.5500.79
235_I243_V0.5490.79
103_F129_A0.5490.79
244_D248_K0.5460.78
202_E214_S0.5450.78
50_A81_M0.5420.78
154_K158_G0.5410.78
185_R188_T0.5360.77
140_G164_P0.5360.77
18_N21_V0.5350.77
59_N136_Y0.5330.77
124_M128_Y0.5330.77
44_V48_V0.5320.76
162_V166_G0.5320.76
92_K95_N0.5280.76
47_A51_T0.5270.76
100_V118_I0.5270.76
67_S122_V0.5260.76
38_I60_F0.5250.76
166_G169_Y0.5250.76
83_K91_Y0.5200.75
73_I78_S0.5200.75
142_G163_L0.5190.75
116_G120_A0.5180.75
117_N120_A0.5170.74
70_T186_I0.5170.74
116_G119_K0.5160.74
103_F118_I0.5150.74
159_G163_L0.5150.74
165_I173_V0.5140.74
40_V81_M0.5130.74
142_G147_L0.5120.74
41_S146_E0.5110.74
15_A19_Y0.5110.74
235_I239_I0.5050.73
120_A153_T0.5040.73
128_Y154_K0.5030.72
227_D230_V0.5020.72
142_G160_V0.5020.72
153_T253_K0.5010.72
16_V19_Y0.5000.72
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3ilvA 2 0.8993 100 0.335 Contact Map
3n05A 2 0.9245 100 0.339 Contact Map
4f4hA 2 0.9281 100 0.342 Contact Map
3sdbA 6 0.9137 100 0.36 Contact Map
1xngA 2 0.9245 100 0.395 Contact Map
1kqpA 2 0.8921 100 0.411 Contact Map
4xfdA 1 0.8201 100 0.413 Contact Map
4q16A 2 0.8957 100 0.416 Contact Map
2e18A 2 0.9101 100 0.419 Contact Map
3q4gA 2 0.8417 100 0.421 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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