Q97WN6 - SULSO - GREMLIN Contacts

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Q97WN6 - Uncharacterized protein

UniProt:	Q97WN6	Sequences: MNGSSIFFIVLYLIFLIIGKIKYVKEAERIVDIVVILLILTISYWAGEEITSNQVFSLVISSLILSIFSIIITYFSGVAIRSFNNSTKLLVKTNKEKVPNRSNRQTIIKYMLPFISGWIFGLLFHINDNIVINLVDYELYALASVLGYTMGKDVNRKVILNSSKDSLISLFITILGDIILALVIYMLHIANLTISFAIALASGWYSYVGPLVAVSSNPYLGTLAFLINFLREQLTYVLVPFLLRVKFEPRSAVAVGGATAMDTTLPLYVEVLGKEYALSAMITGVILTLIIPIILPLII	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	299 (289)
Sequences:	384 (267)
Seq/√Len:	15.7

ECOLI - YBJE
SULSO - Q97WN6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
256_G	260_A	4.113	1.00
260_A	283_T	4.002	1.00
185_Y	195_S	3.779	1.00
259_T	264_T	3.655	1.00
115_I	286_I	3.293	0.99
68_F	180_L	3.272	0.99
212_V	295_L	3.176	0.99
59_V	247_F	3.020	0.98
205_Y	240_P	2.911	0.98
68_F	184_I	2.774	0.97
42_I	225_F	2.703	0.96
234_L	253_V	2.680	0.96
166_S	170_L	2.586	0.95
208_V	228_N	2.572	0.95
180_L	184_I	2.552	0.95
77_G	254_A	2.458	0.94
72_I	183_V	2.400	0.93
172_I	192_L	2.384	0.93
5_S	142_L	2.358	0.92
121_G	205_Y	2.355	0.92
283_T	288_T	2.313	0.92
283_T	287_L	2.308	0.92
218_P	225_F	2.248	0.91
268_Y	276_Y	2.244	0.90
146_L	261_M	2.225	0.90
146_L	255_V	2.212	0.90
73_T	176_G	2.185	0.89
43_S	207_Y	2.162	0.89
200_L	207_Y	2.146	0.88
47_G	136_D	2.139	0.88
84_N	183_V	2.102	0.87
235_T	256_G	2.098	0.87
37_L	67_I	2.084	0.87
258_A	291_I	2.035	0.85
200_L	298_I	1.987	0.84
67_I	71_I	1.886	0.81
141_A	189_I	1.869	0.80
261_M	288_T	1.824	0.78
76_S	176_G	1.725	0.74
212_V	220_L	1.704	0.73
27_A	191_N	1.691	0.73
121_G	240_P	1.691	0.73
44_Y	75_F	1.686	0.72
179_I	286_I	1.677	0.72
282_I	288_T	1.641	0.70
66_S	70_I	1.640	0.70
143_A	288_T	1.629	0.70
39_I	180_L	1.626	0.70
198_I	291_I	1.626	0.70
40_L	180_L	1.592	0.68
107_I	205_Y	1.591	0.68
75_F	183_V	1.577	0.67
150_M	213_A	1.555	0.66
79_A	179_I	1.554	0.66
62_S	65_L	1.545	0.65
38_L	176_G	1.545	0.65
269_V	272_L	1.516	0.64
142_L	297_L	1.516	0.64
110_Y	113_P	1.495	0.63
115_I	171_F	1.495	0.63
63_L	171_F	1.454	0.61
220_L	295_L	1.454	0.61
211_L	238_L	1.438	0.60
110_Y	229_F	1.431	0.59
173_T	287_L	1.401	0.58
77_G	218_P	1.398	0.57
225_F	270_E	1.396	0.57
43_S	200_L	1.368	0.56
42_I	110_Y	1.363	0.55
93_T	96_E	1.360	0.55
181_A	195_S	1.356	0.55
208_V	292_P	1.353	0.55
235_T	278_L	1.353	0.55
262_D	265_L	1.347	0.55
253_V	276_Y	1.332	0.54
8_F	31_V	1.331	0.54
42_I	217_N	1.319	0.53
209_G	288_T	1.310	0.52
244_R	247_F	1.307	0.52
36_I	225_F	1.307	0.52
82_S	179_I	1.306	0.52
101_R	141_A	1.301	0.52
64_I	237_V	1.281	0.51
262_D	284_G	1.280	0.51
178_I	182_L	1.265	0.50
35_V	210_P	1.252	0.49
149_T	266_P	1.252	0.49
114_F	233_Q	1.233	0.48
121_G	225_F	1.225	0.48
114_F	260_A	1.221	0.47
66_S	230_L	1.220	0.47
126_I	297_L	1.217	0.47
235_T	295_L	1.213	0.47
120_F	123_L	1.209	0.47
259_T	273_G	1.200	0.46
167_L	171_F	1.198	0.46
211_L	289_L	1.186	0.45
40_L	275_E	1.179	0.45
178_I	294_I	1.179	0.45
151_G	176_G	1.167	0.44
31_V	213_A	1.158	0.44
34_V	236_Y	1.148	0.43
131_V	261_M	1.144	0.43
178_I	199_A	1.130	0.42
193_T	236_Y	1.127	0.42
71_I	141_A	1.126	0.42
283_T	296_P	1.124	0.42
67_I	89_L	1.120	0.42
174_I	290_I	1.113	0.41
213_A	293_I	1.104	0.41
190_A	288_T	1.102	0.41
115_I	179_I	1.078	0.39
46_A	272_L	1.075	0.39
196_F	298_I	1.074	0.39
58_L	206_S	1.074	0.39
269_V	280_A	1.073	0.39
115_I	120_F	1.070	0.39
182_L	186_M	1.064	0.38
63_L	119_I	1.063	0.38
38_L	73_T	1.061	0.38
39_I	229_F	1.060	0.38
145_V	169_S	1.057	0.38
243_L	276_Y	1.050	0.38
47_G	122_L	1.048	0.38
66_S	280_A	1.044	0.37
146_L	180_L	1.043	0.37
49_E	110_Y	1.037	0.37
9_I	297_L	1.036	0.37
228_N	291_I	1.034	0.37
17_I	59_V	1.031	0.37
137_Y	214_V	1.021	0.36
107_I	110_Y	1.011	0.36
94_N	112_L	1.010	0.36
243_L	261_M	1.008	0.35
119_I	175_L	1.006	0.35
265_L	284_G	1.006	0.35
107_I	240_P	1.006	0.35
209_G	268_Y	1.003	0.35
173_T	244_R	1.002	0.35
33_I	112_L	1.002	0.35
27_A	30_I	1.001	0.35
179_I	182_L	1.000	0.35
29_R	187_L	1.000	0.35
25_K	29_R	0.997	0.35
70_I	74_Y	0.994	0.35
38_L	263_T	0.989	0.35
119_I	179_I	0.985	0.34
110_Y	225_F	0.983	0.34
222_T	252_A	0.980	0.34
240_P	243_L	0.979	0.34
282_I	285_V	0.976	0.34
71_I	75_F	0.971	0.34
160_L	280_A	0.967	0.33
249_P	253_V	0.966	0.33
13_L	167_L	0.964	0.33
145_V	236_Y	0.962	0.33
21_I	171_F	0.958	0.33
62_S	294_I	0.954	0.33
166_S	273_G	0.951	0.33
71_I	84_N	0.946	0.32
240_P	269_V	0.945	0.32
143_A	204_W	0.944	0.32
117_G	265_L	0.943	0.32
13_L	141_A	0.936	0.32
269_V	277_A	0.931	0.32
151_G	228_N	0.931	0.32
93_T	241_F	0.931	0.32
31_V	291_I	0.930	0.32
31_V	253_V	0.929	0.31
151_G	243_L	0.923	0.31
120_F	179_I	0.920	0.31
194_I	260_A	0.919	0.31
22_K	183_V	0.919	0.31
243_L	272_L	0.913	0.31
140_Y	215_S	0.906	0.30
92_K	95_K	0.903	0.30
37_L	125_H	0.901	0.30
134_L	172_I	0.899	0.30
156_R	161_N	0.898	0.30
57_S	144_S	0.895	0.30
194_I	212_V	0.895	0.30
134_L	187_L	0.895	0.30
174_I	199_A	0.893	0.30
82_S	105_Q	0.892	0.30
91_V	119_I	0.882	0.29
209_G	261_M	0.880	0.29
80_I	172_I	0.872	0.29
6_I	42_I	0.872	0.29
181_A	217_N	0.870	0.29
224_A	228_N	0.867	0.28
64_I	184_I	0.861	0.28
175_L	182_L	0.859	0.28
39_I	208_V	0.854	0.28
181_A	185_Y	0.851	0.28
116_S	193_T	0.845	0.27
45_W	170_L	0.841	0.27
115_I	187_L	0.839	0.27
15_F	211_L	0.837	0.27
43_S	233_Q	0.836	0.27
173_T	283_T	0.836	0.27
172_I	297_L	0.834	0.27
67_I	182_L	0.834	0.27
236_Y	271_V	0.833	0.27
119_I	123_L	0.833	0.27
122_L	233_Q	0.824	0.26
272_L	278_L	0.823	0.26
58_L	81_R	0.822	0.26
208_V	224_A	0.822	0.26
17_I	83_F	0.819	0.26
39_I	264_T	0.818	0.26
53_N	175_L	0.818	0.26
116_S	187_L	0.814	0.26
91_V	94_N	0.812	0.26
184_I	191_N	0.811	0.26
77_G	150_M	0.810	0.26
73_T	151_G	0.809	0.26
141_A	175_L	0.808	0.26
268_Y	283_T	0.808	0.26
166_S	181_A	0.808	0.26
79_A	182_L	0.807	0.26
213_A	253_V	0.806	0.26
224_A	227_I	0.804	0.26
194_I	216_S	0.801	0.25
15_F	298_I	0.797	0.25
23_Y	94_N	0.791	0.25
129_N	175_L	0.790	0.25
101_R	133_N	0.790	0.25
41_T	197_A	0.785	0.25
72_I	179_I	0.785	0.25
30_I	93_T	0.784	0.25
116_S	127_N	0.781	0.25
141_A	268_Y	0.779	0.25
257_G	262_D	0.779	0.25
13_L	112_L	0.779	0.25
220_L	224_A	0.778	0.24
8_F	228_N	0.776	0.24
137_Y	258_A	0.774	0.24
144_S	183_V	0.772	0.24
222_T	276_Y	0.772	0.24
261_M	268_Y	0.772	0.24
15_F	51_T	0.771	0.24
164_K	230_L	0.763	0.24
15_F	280_A	0.760	0.24
31_V	236_Y	0.759	0.24
60_I	95_K	0.755	0.24
102_S	174_I	0.754	0.23
15_F	238_L	0.751	0.23
132_I	137_Y	0.750	0.23
188_H	210_P	0.735	0.23
16_L	68_F	0.733	0.23
63_L	137_Y	0.733	0.23
253_V	283_T	0.732	0.23
43_S	77_G	0.730	0.23
114_F	254_A	0.730	0.23
72_I	171_F	0.729	0.22
91_V	175_L	0.729	0.22
51_T	263_T	0.728	0.22
258_A	287_L	0.727	0.22
94_N	127_N	0.724	0.22
183_V	237_V	0.724	0.22
226_L	264_T	0.721	0.22
110_Y	240_P	0.721	0.22
30_I	132_I	0.720	0.22
146_L	165_D	0.718	0.22
202_S	207_Y	0.717	0.22
81_R	236_Y	0.717	0.22
209_G	221_G	0.711	0.22
77_G	176_G	0.710	0.22
280_A	287_L	0.709	0.22
103_N	167_L	0.708	0.22
226_L	230_L	0.707	0.22
151_G	170_L	0.705	0.22
107_I	149_T	0.697	0.21
178_I	202_S	0.697	0.21
255_V	260_A	0.694	0.21
156_R	228_N	0.694	0.21
14_I	207_Y	0.692	0.21
179_I	183_V	0.691	0.21
222_T	243_L	0.690	0.21
75_F	171_F	0.686	0.21
265_L	292_P	0.686	0.21
34_V	200_L	0.686	0.21
221_G	259_T	0.685	0.21
116_S	290_I	0.685	0.21
186_M	241_F	0.680	0.21
79_A	124_F	0.678	0.20
66_S	192_L	0.678	0.20
70_I	253_V	0.678	0.20
186_M	192_L	0.675	0.20
58_L	191_N	0.674	0.20
141_A	191_N	0.673	0.20
204_W	226_L	0.673	0.20
58_L	209_G	0.672	0.20
222_T	227_I	0.671	0.20
55_V	240_P	0.667	0.20
20_K	115_I	0.667	0.20
78_V	119_I	0.667	0.20
12_Y	37_L	0.665	0.20
37_L	187_L	0.663	0.20
115_I	133_N	0.663	0.20
198_I	235_T	0.662	0.20
147_G	232_E	0.662	0.20
112_L	171_F	0.662	0.20
213_A	222_T	0.661	0.20
75_F	116_S	0.660	0.20
77_G	166_S	0.655	0.20
65_L	184_I	0.653	0.20
205_Y	273_G	0.652	0.20
247_F	289_L	0.651	0.19
22_K	120_F	0.651	0.19
51_T	109_K	0.648	0.19
27_A	75_F	0.644	0.19
12_Y	48_E	0.641	0.19
30_I	35_V	0.640	0.19
41_T	275_E	0.640	0.19
168_I	171_F	0.638	0.19
9_I	30_I	0.636	0.19
55_V	205_Y	0.635	0.19
166_S	285_V	0.634	0.19
112_L	116_S	0.632	0.19
243_L	248_E	0.628	0.19
228_N	278_L	0.628	0.19
146_L	283_T	0.628	0.19
205_Y	263_T	0.626	0.19
194_I	263_T	0.626	0.19
36_I	46_A	0.625	0.19
30_I	293_I	0.624	0.19
261_M	283_T	0.623	0.19
11_L	14_I	0.623	0.19
5_S	297_L	0.623	0.19
172_I	182_L	0.622	0.18
31_V	36_I	0.621	0.18
60_I	223_L	0.621	0.18
21_I	234_L	0.616	0.18
89_L	234_L	0.616	0.18
117_G	221_G	0.615	0.18
145_V	150_M	0.614	0.18
282_I	287_L	0.614	0.18
171_F	183_V	0.612	0.18
169_S	255_V	0.611	0.18
50_I	240_P	0.611	0.18
98_V	191_N	0.610	0.18
82_S	241_F	0.609	0.18
242_L	290_I	0.608	0.18
100_N	226_L	0.606	0.18
151_G	240_P	0.604	0.18
94_N	101_R	0.604	0.18
11_L	285_V	0.604	0.18
210_P	235_T	0.602	0.18
209_G	264_T	0.602	0.18
225_F	247_F	0.601	0.18
116_S	179_I	0.601	0.18
147_G	206_S	0.601	0.18
17_I	91_V	0.601	0.18
163_S	228_N	0.601	0.18
6_I	141_A	0.598	0.18
63_L	167_L	0.596	0.18
167_L	288_T	0.595	0.18
53_N	64_I	0.594	0.18
174_I	287_L	0.593	0.17
212_V	224_A	0.592	0.17
75_F	175_L	0.590	0.17
101_R	119_I	0.588	0.17
223_L	256_G	0.587	0.17
163_S	278_L	0.584	0.17
133_N	267_L	0.583	0.17
114_F	229_F	0.582	0.17
80_I	112_L	0.580	0.17
148_Y	289_L	0.577	0.17
95_K	179_I	0.570	0.17
64_I	146_L	0.570	0.17
110_Y	170_L	0.569	0.17
140_Y	250_R	0.568	0.17
182_L	192_L	0.568	0.17
151_G	225_F	0.566	0.17
272_L	288_T	0.565	0.17
25_K	89_L	0.562	0.16
78_V	123_L	0.562	0.16
45_W	218_P	0.560	0.16
37_L	116_S	0.560	0.16
8_F	80_I	0.559	0.16
212_V	279_S	0.558	0.16
42_I	233_Q	0.558	0.16
56_F	126_I	0.556	0.16
41_T	230_L	0.555	0.16
154_V	239_V	0.551	0.16
155_N	228_N	0.551	0.16
210_P	263_T	0.550	0.16
17_I	27_A	0.547	0.16
258_A	283_T	0.545	0.16
65_L	234_L	0.545	0.16
248_E	295_L	0.544	0.16
31_V	263_T	0.543	0.16
67_I	234_L	0.543	0.16
8_F	291_I	0.541	0.16
9_I	56_F	0.541	0.16
109_K	200_L	0.541	0.16
56_F	223_L	0.540	0.16
13_L	97_K	0.538	0.16
175_L	186_M	0.537	0.16
98_V	167_L	0.534	0.16
225_F	240_P	0.533	0.16
40_L	115_I	0.533	0.16
236_Y	289_L	0.531	0.16
17_I	92_K	0.531	0.16
58_L	252_A	0.530	0.15
187_L	252_A	0.527	0.15
175_L	275_E	0.526	0.15
10_V	166_S	0.526	0.15
63_L	89_L	0.523	0.15
74_Y	251_S	0.522	0.15
119_I	237_V	0.520	0.15
183_V	192_L	0.520	0.15
13_L	37_L	0.520	0.15
46_A	77_G	0.519	0.15
286_I	298_I	0.519	0.15
52_S	240_P	0.519	0.15
44_Y	271_V	0.519	0.15
175_L	184_I	0.516	0.15
152_K	212_V	0.514	0.15
170_L	243_L	0.512	0.15
67_I	174_I	0.511	0.15
38_L	181_A	0.510	0.15
38_L	151_G	0.506	0.15
18_I	111_M	0.505	0.15
266_P	277_A	0.503	0.15
92_K	123_L	0.503	0.15
131_V	242_L	0.503	0.15
268_Y	279_S	0.501	0.15
144_S	148_Y	0.501	0.15
35_V	263_T	0.501	0.15
110_Y	272_L	0.501	0.15
127_N	179_I	0.500	0.15
176_G	228_N	0.500	0.15

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3zuxA	1	0.5518	71.6	0.95	Contact Map
4bwzA	2	0.6555	70.6	0.95	Contact Map
4cz8A	2	0.6957	70.4	0.95	Contact Map
4n7wA	2	0.5585	50.8	0.956	Contact Map
4czbA	2	0.6522	45.3	0.958	Contact Map
3dh4A	3	0.3579	15.7	0.966	Contact Map
3giaA	1	0.2943	13.7	0.967	Contact Map
2xq2A	2	0.4716	9.2	0.97	Contact Map
3qnqA	2	0.2943	8.8	0.97	Contact Map
3l1lA	2	0.2709	8.3	0.97	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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