Q97WM3 - SULSO - GREMLIN Contacts

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Q97WM3 - Uncharacterized protein

UniProt:	Q97WM3	Sequences: MLILSKRLIDILSESLSLKEAYTQYKNKNLNYALKLLDELITQNPDLKEAYSLKGKILFELGKLQEAYENFQKAEDKIGMAKVLIAQGLYRDALIYLNDNSKDSRKLRGLIYLKLENYEKALDEIKVVEDEDPLVYKIRGISELKLGKYNDAIIDLSLAIEKYPTDAELYFYRALAQRELGNYKEAENDLEIAINLNPYYAEVYFSLGELKESRGLLEEAVDMYSKSININPLYRNAYVRRAKSLMKLGREEEAYADIRKANELNSS	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	267 (219)
Sequences:	28740 (25187)
Seq/√Len:	1702.0

ECOLI - LPSRP HEMY YBEQ NLPI PGAA YPHG YJCO YFBP BCSC BAMD YGEH NRFG
BACSU - YVCD YPIA RAPE RAPB RAPG RAPH RAPJ RAPD RAPK RAPA RAPF RAPI RAPC YRRB YSOA GUTR
HALSA - Q9HMM8
SULSO - Q97WM3 Q97V36 Q97VC4 Q97ZS3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
216_L	219_E	3.432	1.00
250_R	253_E	3.381	1.00
148_K	151_D	3.363	1.00
182_N	185_E	3.347	1.00
117_N	120_K	3.129	1.00
63_K	66_E	2.997	1.00
29_N	32_Y	2.854	1.00
204_Y	223_M	2.609	1.00
170_Y	189_D	2.364	1.00
140_G	156_L	2.231	1.00
242_A	258_I	2.207	1.00
238_Y	257_D	2.177	1.00
174_A	190_L	2.161	1.00
208_G	224_Y	2.132	1.00
136_Y	155_D	2.106	1.00
207_L	223_M	2.043	1.00
191_E	207_L	2.023	1.00
211_K	223_M	1.923	1.00
51_Y	70_N	1.879	1.00
109_G	125_I	1.875	1.00
177_Q	189_D	1.870	1.00
245_L	257_D	1.851	1.00
211_K	219_E	1.846	1.00
55_G	71_F	1.815	1.00
173_R	189_D	1.800	1.00
139_R	155_D	1.770	1.00
187_E	191_E	1.769	1.00
157_S	173_R	1.757	1.00
143_E	155_D	1.723	1.00
105_R	124_E	1.696	1.00
221_V	225_S	1.695	1.00
21_A	37_L	1.685	1.00
156_L	172_Y	1.658	1.00
38_D	54_K	1.633	1.00
143_E	151_D	1.600	1.00
153_I	157_S	1.599	1.00
225_S	241_R	1.573	1.00
126_K	139_R	1.564	1.00
241_R	245_L	1.546	1.00
177_Q	185_E	1.541	1.00
122_L	126_K	1.531	1.00
224_Y	240_R	1.527	1.00
34_L	38_D	1.517	1.00
58_L	70_N	1.515	1.00
112_Y	124_E	1.511	1.00
190_L	206_S	1.495	1.00
241_R	257_D	1.493	1.00
112_Y	120_K	1.490	1.00
139_R	143_E	1.468	1.00
187_E	211_K	1.464	1.00
211_K	216_L	1.455	1.00
24_Q	36_L	1.445	1.00
112_Y	117_N	1.409	1.00
245_L	253_E	1.388	1.00
54_K	58_L	1.374	1.00
221_V	245_L	1.353	1.00
58_L	66_E	1.352	1.00
20_E	24_Q	1.352	1.00
54_K	70_N	1.349	1.00
177_Q	182_N	1.345	1.00
153_I	177_Q	1.344	1.00
24_Q	32_Y	1.336	1.00
245_L	250_R	1.332	1.00
187_E	214_R	1.320	1.00
207_L	211_K	1.312	1.00
221_V	248_L	1.306	1.00
231_N	234_Y	1.302	1.00
173_R	177_Q	1.299	1.00
20_E	36_L	1.298	1.00
34_L	58_L	1.296	1.00
159_A	169_L	1.278	1.00
227_S	237_A	1.272	1.00
108_R	124_E	1.259	1.00
193_A	203_V	1.258	1.00
140_G	152_A	1.256	1.00
143_E	148_K	1.234	1.00
37_L	53_L	1.229	1.00
106_K	135_V	1.223	1.00
108_R	112_Y	1.217	1.00
40_L	50_A	1.216	1.00
58_L	63_K	1.215	1.00
23_T	27_N	1.213	1.00
226_K	230_I	1.191	1.00
24_Q	29_N	1.185	1.00
136_Y	158_L	1.175	1.00
209_E	240_R	1.170	1.00
239_V	243_K	1.165	1.00
68_Y	72_Q	1.164	1.00
205_F	237_A	1.156	1.00
204_Y	226_K	1.133	1.00
205_F	240_R	1.126	1.00
25_Y	57_I	1.102	1.00
120_K	123_D	1.092	1.00
105_R	127_V	1.088	1.00
122_L	143_E	1.072	1.00
105_R	128_V	1.069	1.00
51_Y	73_K	1.069	1.00
170_Y	192_I	1.054	1.00
137_K	172_Y	1.051	1.00
111_I	115_L	1.046	1.00
151_D	154_I	1.045	1.00
137_K	169_L	1.045	1.00
219_E	222_D	1.043	1.00
51_Y	74_A	1.039	1.00
125_I	138_I	1.038	1.00
158_L	162_K	1.031	1.00
228_I	238_Y	1.028	1.00
171_F	206_S	1.019	1.00
155_D	158_L	1.016	1.00
22_Y	26_K	1.016	1.00
178_R	210_L	1.012	1.00
171_F	203_V	1.008	1.00
208_G	227_S	1.001	1.00
170_Y	193_A	0.999	1.00
185_E	188_N	0.990	1.00
192_I	196_L	0.988	1.00
197_N	200_Y	0.986	1.00
136_Y	159_A	0.981	1.00
113_L	142_S	0.978	1.00
153_I	180_L	0.975	1.00
55_G	67_A	0.971	1.00
226_K	229_N	0.968	1.00
25_Y	53_L	0.968	1.00
253_E	256_A	0.958	1.00
175_L	206_S	0.952	1.00
109_G	121_A	0.947	1.00
70_N	73_K	0.947	1.00
153_I	173_R	0.942	1.00
205_F	209_E	0.941	1.00
208_G	220_A	0.932	1.00
212_E	240_R	0.931	1.00
140_G	159_A	0.930	1.00
246_M	258_I	0.924	1.00
204_Y	227_S	0.918	1.00
149_Y	176_A	0.916	1.00
110_L	138_I	0.914	1.00
187_E	207_L	0.909	1.00
246_M	251_E	0.908	1.00
178_R	206_S	0.908	1.00
206_S	209_E	0.902	1.00
174_A	186_A	0.897	1.00
175_L	179_E	0.895	1.00
32_Y	35_K	0.890	1.00
66_E	69_E	0.888	1.00
73_K	77_K	0.888	1.00
36_L	39_E	0.885	1.00
122_L	146_L	0.880	1.00
34_L	61_L	0.879	1.00
209_E	213_S	0.875	1.00
52_S	56_K	0.875	1.00
221_V	241_R	0.875	1.00
109_G	128_V	0.868	1.00
171_F	175_L	0.865	1.00
141_I	172_Y	0.860	1.00
56_K	60_E	0.858	1.00
122_L	139_R	0.857	1.00
194_I	204_Y	0.856	1.00
55_G	74_A	0.856	1.00
174_A	193_A	0.839	1.00
220_A	244_S	0.837	1.00
200_Y	203_V	0.831	1.00
221_V	244_S	0.830	1.00
160_I	170_Y	0.827	1.00
184_K	214_R	0.826	1.00
53_L	56_K	0.819	1.00
21_A	40_L	0.817	1.00
217_L	248_L	0.817	1.00
242_A	254_A	0.817	1.00
124_E	127_V	0.815	1.00
217_L	244_S	0.809	1.00
20_E	23_T	0.798	1.00
172_Y	175_L	0.797	1.00
22_Y	53_L	0.795	1.00
64_L	68_Y	0.791	1.00
187_E	210_L	0.791	1.00
212_E	247_K	0.791	1.00
136_Y	162_K	0.790	1.00
110_L	114_K	0.789	1.00
141_I	145_K	0.787	1.00
189_D	192_I	0.786	1.00
106_K	110_L	0.786	1.00
201_A	227_S	0.785	1.00
153_I	176_A	0.781	1.00
193_A	196_L	0.780	1.00
59_F	67_A	0.777	1.00
48_K	52_S	0.777	1.00
118_Y	142_S	0.771	1.00
142_S	146_L	0.771	1.00
218_E	248_L	0.763	1.00
107_L	110_L	0.763	1.00
30_L	57_I	0.759	1.00
113_L	121_A	0.758	1.00
34_L	54_K	0.756	1.00
34_L	57_I	0.755	1.00
192_I	195_N	0.755	1.00
159_A	162_K	0.755	1.00
204_Y	230_I	0.749	1.00
228_I	237_A	0.749	1.00
44_N	47_L	0.747	1.00
158_L	161_E	0.746	1.00
183_Y	214_R	0.746	1.00
113_L	118_Y	0.745	1.00
150_N	180_L	0.741	1.00
234_Y	237_A	0.740	1.00
128_V	135_V	0.735	1.00
166_D	169_L	0.731	1.00
59_F	64_L	0.730	1.00
39_E	43_Q	0.730	1.00
149_Y	153_I	0.729	1.00
240_R	243_K	0.729	1.00
106_K	138_I	0.728	1.00
102_K	128_V	0.724	1.00
183_Y	210_L	0.723	1.00
52_S	55_G	0.721	1.00
160_I	173_R	0.716	1.00
41_I	51_Y	0.712	1.00
223_M	226_K	0.712	1.00
21_A	33_A	0.710	1.00
144_L	172_Y	0.709	1.00
154_I	158_L	0.708	1.00
167_A	193_A	0.706	1.00
33_A	57_I	0.702	1.00
176_A	180_L	0.701	1.00
212_E	220_A	0.700	1.00
210_L	214_R	0.698	1.00
246_M	254_A	0.696	1.00
244_S	248_L	0.694	1.00
217_L	247_K	0.693	1.00
19_K	23_T	0.693	1.00
159_A	163_Y	0.686	1.00
144_L	152_A	0.686	1.00
170_Y	196_L	0.685	1.00
194_I	203_V	0.682	1.00
212_E	217_L	0.682	1.00
201_A	205_F	0.681	1.00
243_K	247_K	0.681	1.00
144_L	149_Y	0.680	1.00
57_I	61_L	0.680	1.00
138_I	141_I	0.672	1.00
239_V	242_A	0.672	1.00
25_Y	30_L	0.670	1.00
40_L	43_Q	0.669	1.00
48_K	74_A	0.666	1.00
191_E	195_N	0.658	1.00
183_Y	187_E	0.653	1.00
212_E	224_Y	0.652	1.00
225_S	229_N	0.649	1.00
41_I	54_K	0.648	1.00
222_D	226_K	0.644	1.00
163_Y	166_D	0.641	1.00
212_E	244_S	0.641	1.00
123_D	127_V	0.640	1.00
137_K	141_I	0.638	1.00
141_I	144_L	0.637	1.00
209_E	212_E	0.636	1.00
155_D	159_A	0.633	1.00
227_S	230_I	0.633	1.00
149_Y	180_L	0.632	1.00
184_K	187_E	0.632	1.00
201_A	231_N	0.631	1.00
202_E	206_S	0.629	1.00
221_V	250_R	0.628	1.00
224_Y	237_A	0.627	1.00
69_E	73_K	0.623	1.00
185_E	189_D	0.622	1.00
144_L	176_A	0.617	1.00
143_E	146_L	0.612	1.00
219_E	223_M	0.602	1.00
251_E	255_Y	0.601	1.00
178_R	186_A	0.601	1.00
24_Q	27_N	0.598	1.00
242_A	246_M	0.598	1.00
25_Y	33_A	0.594	1.00
35_K	39_E	0.593	1.00
72_Q	75_E	0.592	1.00
178_R	190_L	0.587	1.00
153_I	182_N	0.580	1.00
113_L	138_I	0.579	1.00
188_N	192_I	0.576	1.00
103_D	107_L	0.575	1.00
242_A	257_D	0.572	1.00
119_E	146_L	0.569	1.00
224_Y	244_S	0.566	1.00
144_L	179_E	0.565	1.00
175_L	178_R	0.564	1.00
66_E	70_N	0.563	1.00
178_R	183_Y	0.562	1.00
160_I	169_L	0.561	1.00
222_D	225_S	0.561	1.00
36_L	40_L	0.561	1.00
151_D	155_D	0.559	1.00
39_E	42_T	0.558	1.00
101_S	104_S	0.558	1.00
51_Y	77_K	0.553	1.00
235_R	239_V	0.552	1.00
73_K	76_D	0.551	1.00
31_N	61_L	0.550	1.00
122_L	151_D	0.550	1.00
243_K	246_M	0.549	1.00
41_I	50_A	0.549	1.00
236_N	240_R	0.548	1.00
122_L	148_K	0.547	1.00
157_S	160_I	0.546	1.00
163_Y	169_L	0.544	1.00
134_L	138_I	0.542	1.00
35_K	38_D	0.542	1.00
253_E	257_D	0.541	1.00
102_K	106_K	0.537	1.00
183_Y	213_S	0.536	1.00
37_L	54_K	0.534	1.00
52_S	74_A	0.531	1.00
40_L	44_N	0.531	1.00
21_A	25_Y	0.529	1.00
235_R	242_A	0.524	1.00
168_E	172_Y	0.520	1.00
184_K	188_N	0.518	1.00
137_K	166_D	0.518	1.00
206_S	210_L	0.517	1.00
201_A	234_Y	0.514	1.00
218_E	221_V	0.513	1.00
209_E	224_Y	0.512	1.00
103_D	106_K	0.511	1.00
47_L	50_A	0.508	1.00
23_T	26_K	0.506	1.00
167_A	200_Y	0.504	1.00
144_L	156_L	0.503	1.00
154_I	157_S	0.503	1.00
55_G	59_F	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1w3bA	2	1	100	0.168	Contact Map
4ui9J	2	1	100	0.189	Contact Map
2xpiA	2	0.9963	100	0.2	Contact Map
3dssA	1	0.9251	100	0.206	Contact Map
4rg9A	2	0.764	100	0.211	Contact Map
4ui9C	2	1	100	0.214	Contact Map
2y4tA	1	1	100	0.215	Contact Map
4l9pA	1	0.9064	100	0.217	Contact Map
4eqfA	1	0.8839	100	0.226	Contact Map
4ui9F	2	0.9213	100	0.227	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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