GREMLIN Database
NUCS - Endonuclease NucS
UniProt: Q97WK8 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 236 (208)
Sequences: 453 (174)
Seq/√Len: 12.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
137_P160_L6.0571.00
203_R225_E5.4341.00
191_Y224_L4.6861.00
60_I66_K4.3601.00
137_P162_G3.9640.99
60_I65_V3.0780.96
59_V102_I2.9250.95
128_D131_N2.6610.92
35_N43_V2.6120.91
46_E105_K2.5490.90
51_I61_I2.5210.90
28_I51_I2.4980.89
184_I215_A2.4400.88
94_N98_E2.4230.87
139_M233_N2.3880.87
137_P229_Y2.3860.87
137_P144_F2.3580.86
136_N139_M2.3510.86
31_T48_P2.3450.86
140_I229_Y2.2750.84
140_I172_I2.2600.83
164_D203_R2.2520.83
60_I63_E2.2050.82
139_M144_F2.0290.76
137_P174_V2.0120.75
205_I225_E2.0020.75
161_I169_F1.9340.72
93_R96_P1.8820.70
93_R98_E1.7920.66
220_Q225_E1.7650.65
109_Y113_A1.7320.63
141_E144_F1.7310.63
31_T105_K1.7310.63
180_Q212_T1.7170.63
172_I205_I1.6870.61
30_A104_L1.6560.60
144_F160_L1.6330.59
52_I65_V1.6040.57
144_F162_G1.5960.57
152_N194_M1.5900.57
48_P108_F1.5860.56
141_E203_R1.5390.54
140_I144_F1.5350.54
98_E103_D1.5260.53
89_I98_E1.5060.52
160_L229_Y1.4730.51
63_E66_K1.4660.50
89_I93_R1.4320.49
27_V50_L1.4230.48
89_I101_E1.3890.47
74_P96_P1.3830.46
138_S142_E1.3430.44
162_G174_V1.3380.44
177_S210_D1.3360.44
143_G164_D1.3360.44
64_S177_S1.3340.44
42_S73_Q1.2870.41
51_I102_I1.2850.41
32_C44_A1.2800.41
140_I225_E1.2780.41
44_A120_F1.2720.41
160_L174_V1.2650.40
147_L161_I1.2620.40
128_D180_Q1.2540.40
92_Q97_K1.2520.40
58_T207_V1.2210.38
41_E136_N1.2210.38
51_I95_R1.2200.38
89_I92_Q1.2050.38
194_M229_Y1.2020.37
94_N102_I1.1900.37
127_I130_V1.1880.37
124_G189_R1.1730.36
101_E131_N1.1690.36
205_I212_T1.1140.33
107_V136_N1.1130.33
89_I96_P1.1000.33
51_I104_L1.0910.32
33_K233_N1.0800.32
100_I218_L1.0780.32
162_G169_F1.0710.31
52_I66_K1.0580.31
60_I95_R1.0460.30
113_A180_Q1.0450.30
74_P180_Q1.0350.30
85_Y93_R1.0200.29
98_E136_N1.0140.29
144_F174_V1.0050.29
50_L95_R1.0010.28
57_G62_H1.0000.28
90_V96_P0.9900.28
147_L169_F0.9870.28
152_N161_I0.9820.28
137_P201_K0.9700.27
162_G229_Y0.9690.27
182_N195_R0.9600.27
34_V44_A0.9580.27
93_R210_D0.9510.26
90_V98_E0.9490.26
74_P93_R0.9480.26
67_R185_S0.9400.26
28_I34_V0.9320.26
30_A51_I0.9110.25
93_R168_N0.9100.25
102_I230_D0.9100.25
25_L35_N0.9090.25
195_R223_G0.9050.25
46_E121_T0.9010.25
34_V62_H0.9000.25
144_F203_R0.8980.24
98_E105_K0.8980.24
106_E138_S0.8980.24
125_R199_G0.8940.24
101_E233_N0.8840.24
37_K99_V0.8800.24
31_T106_E0.8800.24
30_A181_L0.8740.24
67_R76_G0.8680.23
216_R223_G0.8670.23
129_I157_K0.8600.23
47_S70_L0.8600.23
140_I160_L0.8590.23
168_N203_R0.8580.23
92_Q98_E0.8580.23
87_L162_G0.8520.23
155_Y193_H0.8390.22
67_R125_R0.8380.22
41_E131_N0.8370.22
37_K111_T0.8260.22
90_V93_R0.8220.22
29_F104_L0.8220.22
93_R137_P0.8210.22
203_R227_I0.8060.21
183_A206_L0.8050.21
34_V57_G0.8040.21
74_P224_L0.8010.21
130_V149_R0.8000.21
48_P112_S0.7930.21
41_E139_M0.7910.21
216_R220_Q0.7870.21
162_G172_I0.7870.21
48_P229_Y0.7860.21
142_E165_K0.7850.20
188_Y222_L0.7800.20
89_I166_K0.7750.20
121_T180_Q0.7720.20
144_F229_Y0.7720.20
33_K46_E0.7700.20
47_S73_Q0.7670.20
139_M162_G0.7650.20
47_S76_G0.7630.20
120_F177_S0.7570.20
195_R221_K0.7540.19
97_K206_L0.7510.19
40_A122_I0.7500.19
42_S209_P0.7500.19
92_Q99_V0.7490.19
145_V163_L0.7470.19
48_P106_E0.7410.19
183_A231_I0.7400.19
91_A98_E0.7400.19
153_T158_I0.7380.19
92_Q96_P0.7330.19
192_L229_Y0.7280.19
144_F172_I0.7240.19
66_K93_R0.7240.19
36_Y71_N0.7170.18
65_V89_I0.7150.18
44_A49_R0.7150.18
86_P98_E0.7130.18
137_P141_E0.7110.18
58_T67_R0.7090.18
111_T167_G0.7030.18
51_I75_P0.7000.18
64_S120_F0.7000.18
182_N215_A0.6980.18
152_N202_I0.6970.18
52_I74_P0.6950.18
88_K96_P0.6940.18
85_Y167_G0.6940.18
86_P89_I0.6930.18
153_T182_N0.6900.18
80_E112_S0.6860.17
131_N140_I0.6780.17
37_K98_E0.6760.17
121_T137_P0.6740.17
128_D174_V0.6680.17
120_F124_G0.6670.17
206_L210_D0.6640.17
158_I218_L0.6640.17
93_R203_R0.6630.17
62_H191_Y0.6630.17
141_E164_D0.6610.17
91_A96_P0.6610.17
48_P117_E0.6570.17
90_V99_V0.6570.17
148_T153_T0.6560.17
45_S160_L0.6540.17
127_I133_I0.6520.16
153_T157_K0.6500.16
83_N88_K0.6490.16
60_I90_V0.6490.16
84_D166_K0.6490.16
53_L103_D0.6480.16
71_N117_E0.6470.16
142_E201_K0.6470.16
116_K222_L0.6450.16
86_P97_K0.6450.16
44_A185_S0.6380.16
46_E102_I0.6380.16
37_K202_I0.6360.16
99_V103_D0.6350.16
37_K131_N0.6340.16
57_G74_P0.6300.16
168_N180_Q0.6270.16
142_E152_N0.6240.16
75_P172_I0.6200.16
37_K95_R0.6180.16
34_V52_I0.6180.16
127_I158_I0.6180.16
48_P98_E0.6160.15
37_K101_E0.6130.15
145_V180_Q0.6130.15
114_E152_N0.6100.15
76_G181_L0.6080.15
163_L169_F0.6080.15
68_E116_K0.6080.15
68_E222_L0.6020.15
37_K169_F0.6020.15
28_I169_F0.6000.15
138_S145_V0.5990.15
52_I145_V0.5990.15
83_N103_D0.5980.15
145_V168_N0.5960.15
93_R180_Q0.5950.15
122_I130_V0.5950.15
62_H177_S0.5940.15
65_V93_R0.5900.15
121_T225_E0.5900.15
124_G158_I0.5890.15
44_A61_I0.5880.15
28_I79_I0.5880.15
98_E162_G0.5870.15
57_G215_A0.5870.15
153_T218_L0.5870.15
152_N217_E0.5860.15
139_M229_Y0.5810.15
36_Y39_R0.5800.15
164_D168_N0.5800.15
50_L55_P0.5800.15
191_Y219_L0.5790.15
37_K233_N0.5740.14
89_I220_Q0.5730.14
99_V107_V0.5720.14
179_A183_A0.5720.14
86_P94_N0.5710.14
103_D145_V0.5700.14
85_Y203_R0.5700.14
154_P158_I0.5690.14
60_I120_F0.5680.14
31_T108_F0.5680.14
131_N180_Q0.5650.14
27_V95_R0.5640.14
195_R229_Y0.5620.14
136_N162_G0.5600.14
144_F225_E0.5590.14
30_A87_L0.5590.14
53_L145_V0.5560.14
77_T145_V0.5520.14
82_M192_L0.5520.14
33_K160_L0.5500.14
206_L212_T0.5490.14
53_L59_V0.5480.14
88_K111_T0.5460.14
107_V171_I0.5440.14
212_T224_L0.5420.14
113_A213_I0.5410.14
71_N218_L0.5380.14
157_K214_H0.5380.14
122_I125_R0.5380.14
68_E119_D0.5370.14
32_C49_R0.5360.13
90_V180_Q0.5360.13
90_V94_N0.5350.13
34_V120_F0.5330.13
55_P133_I0.5330.13
100_I210_D0.5320.13
205_I227_I0.5320.13
133_I146_P0.5310.13
97_K136_N0.5290.13
162_G202_I0.5280.13
66_K182_N0.5270.13
76_G214_H0.5270.13
66_K139_M0.5210.13
143_G165_K0.5210.13
111_T142_E0.5190.13
172_I207_V0.5180.13
28_I42_S0.5180.13
119_D216_R0.5170.13
127_I154_P0.5160.13
87_L107_V0.5140.13
177_S218_L0.5120.13
61_I101_E0.5090.13
31_T46_E0.5070.13
115_V223_G0.5050.13
194_M223_G0.5050.13
153_T214_H0.5040.13
42_S71_N0.5020.13
41_E128_D0.5010.13
51_I163_L0.5010.13
147_L193_H0.5010.13
86_P145_V0.5000.13
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2vldA 2 0.9492 100 0.276 Contact Map
1y88A 5 0.4407 96.9 0.923 Contact Map
2wcwA 2 0.4068 96 0.931 Contact Map
3h1tA 1 0.4025 94.9 0.936 Contact Map
1gefA 2 0.4237 92.2 0.942 Contact Map
2eo0A 2 0.428 91.8 0.943 Contact Map
1ob8A 1 0.3898 88.5 0.947 Contact Map
4f0qA 4 0.9237 88.4 0.947 Contact Map
1hh1A 2 0.4068 87.6 0.948 Contact Map
3fovA 2 0.3771 87.3 0.948 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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