Y2213 - SULSO - GREMLIN Contacts

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Y2213 - UPF0284 protein SSO2213

UniProt:	Q97WK3	Sequences: MIKAYFGAETFTLNKDFAYILVIGTTDVSLIPGLTIAGATPELTHFTPAADAEYVILGKCKSINTIPVSPTGIPTPALLTRASLSFTKSLKIVVNAGSRITPKIPYIDLQGEPGKDIRKQALSIEKVNIIIENGIKLGEELSNEYELIMIGESIPAGTTTAMATLLALGYDAMDKVSSASPDNPKELKRKVVEEALRNLPTDPLQRLAKVSDPVLLGVAGISLGFRGKILLAGGTQMTAAAAIVNEFDKSKLKDIIIGTTKWIVEDKSADMLSLAKQVGVKVLASMLDLSISIHEGIRTYEKGYVKEGVGAGGSTIMAFIRGVSNSTLVRKIDELYSELVGSNNLNT	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	347 (320)
Sequences:	313 (233)
Seq/√Len:	13.0

HALSA - Y1572
SULSO - Y2213

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
270_D	273_S	5.921	1.00
167_A	206_R	5.188	1.00
222_S	244_V	4.237	1.00
127_V	208_A	3.690	0.99
32_P	41_P	3.500	0.99
22_V	218_V	3.433	0.99
287_L	315_T	3.397	0.98
19_Y	310_G	3.157	0.98
196_L	210_V	3.021	0.97
23_I	153_S	2.988	0.97
118_R	191_V	2.935	0.96
166_L	189_R	2.882	0.96
261_K	265_E	2.768	0.95
135_I	223_L	2.619	0.93
329_V	333_D	2.575	0.92
163_A	195_A	2.404	0.89
39_A	50_A	2.398	0.89
328_L	332_I	2.372	0.88
150_I	313_G	2.357	0.88
238_T	275_A	2.350	0.88
264_V	272_L	2.311	0.87
164_T	206_R	2.294	0.87
262_W	287_L	2.203	0.84
232_A	313_G	2.187	0.84
79_L	311_A	2.167	0.83
258_G	316_I	2.054	0.80
172_A	175_K	2.053	0.80
95_N	108_D	2.045	0.79
162_M	172_A	2.030	0.79
262_W	301_E	1.993	0.78
39_A	199_L	1.974	0.77
237_M	240_A	1.964	0.76
222_S	255_I	1.961	0.76
245_N	278_V	1.953	0.76
108_D	111_G	1.947	0.76
276_K	313_G	1.935	0.75
215_L	240_A	1.927	0.75
28_V	117_I	1.924	0.75
214_V	218_V	1.900	0.74
24_G	214_V	1.882	0.73
114_G	183_N	1.849	0.72
114_G	213_P	1.833	0.71
35_T	156_A	1.825	0.71
84_L	89_S	1.816	0.70
202_D	205_Q	1.816	0.70
80_T	322_G	1.791	0.69
148_I	220_G	1.726	0.66
21_L	52_A	1.723	0.66
80_T	314_S	1.723	0.66
177_S	183_N	1.684	0.64
156_A	217_G	1.671	0.64
192_V	196_L	1.642	0.62
16_D	326_S	1.627	0.62
230_L	258_G	1.600	0.60
96_A	109_L	1.598	0.60
23_I	98_S	1.594	0.60
55_V	163_A	1.592	0.60
134_G	221_I	1.576	0.59
69_S	75_T	1.556	0.58
92_I	137_L	1.537	0.57
238_T	271_M	1.524	0.56
48_P	118_R	1.491	0.55
271_M	275_A	1.479	0.54
154_I	214_V	1.478	0.54
167_A	264_V	1.459	0.53
155_P	184_P	1.443	0.52
55_V	309_V	1.436	0.52
266_D	306_K	1.428	0.51
20_I	139_E	1.423	0.51
75_T	154_I	1.419	0.51
28_V	210_V	1.412	0.51
238_T	274_L	1.411	0.51
313_G	322_G	1.394	0.50
331_K	334_E	1.379	0.49
73_I	262_W	1.369	0.48
95_N	102_P	1.361	0.48
28_V	179_A	1.346	0.47
96_A	217_G	1.324	0.46
50_A	63_I	1.317	0.46
42_E	314_S	1.313	0.45
194_E	276_K	1.292	0.44
221_I	224_G	1.289	0.44
273_S	277_Q	1.288	0.44
177_S	269_A	1.287	0.44
148_I	225_F	1.287	0.44
134_G	320_I	1.284	0.44
189_R	193_E	1.283	0.44
141_L	172_A	1.273	0.43
109_L	257_I	1.269	0.43
139_E	227_G	1.261	0.43
163_A	222_S	1.252	0.42
241_A	255_I	1.249	0.42
96_A	134_G	1.249	0.42
297_I	322_G	1.247	0.42
73_I	299_T	1.245	0.42
26_T	37_A	1.244	0.42
179_A	243_I	1.241	0.42
177_S	271_M	1.233	0.41
79_L	315_T	1.232	0.41
40_T	44_T	1.221	0.41
203_P	287_L	1.214	0.40
36_I	179_A	1.209	0.40
168_L	242_A	1.208	0.40
44_T	69_S	1.205	0.40
121_A	208_A	1.194	0.39
131_I	220_G	1.192	0.39
178_S	266_D	1.181	0.39
263_I	271_M	1.163	0.38
58_G	274_L	1.152	0.37
181_P	264_V	1.139	0.37
261_K	301_E	1.138	0.37
92_I	107_I	1.137	0.37
75_T	114_G	1.136	0.36
293_I	337_S	1.135	0.36
43_L	314_S	1.135	0.36
163_A	192_V	1.128	0.36
141_L	242_A	1.123	0.36
203_P	246_E	1.122	0.36
232_A	320_I	1.113	0.35
24_G	178_S	1.109	0.35
131_I	204_L	1.109	0.35
112_E	115_K	1.099	0.35
105_P	296_G	1.099	0.35
235_T	332_I	1.096	0.35
23_I	311_A	1.096	0.35
83_S	264_V	1.090	0.34
159_T	333_D	1.085	0.34
110_Q	113_P	1.082	0.34
39_A	43_L	1.072	0.33
81_R	85_S	1.070	0.33
164_T	211_S	1.068	0.33
199_L	280_V	1.066	0.33
163_A	167_A	1.064	0.33
34_L	195_A	1.061	0.33
75_T	315_T	1.051	0.32
137_L	184_P	1.049	0.32
17_F	21_L	1.043	0.32
213_P	239_A	1.042	0.32
132_E	222_S	1.037	0.32
27_D	115_K	1.033	0.32
230_L	271_M	1.033	0.32
150_I	225_F	1.032	0.32
56_I	123_S	1.029	0.31
113_P	255_I	1.024	0.31
190_K	200_P	1.022	0.31
114_G	257_I	1.019	0.31
154_I	263_I	1.019	0.31
23_I	104_I	1.019	0.31
112_E	123_S	1.015	0.31
142_S	314_S	1.009	0.31
73_I	116_D	0.999	0.30
44_T	48_P	0.991	0.30
190_K	194_E	0.987	0.30
21_L	90_L	0.987	0.30
57_L	61_K	0.986	0.30
193_E	282_V	0.985	0.30
201_T	205_Q	0.985	0.30
142_S	317_M	0.984	0.29
110_Q	335_L	0.981	0.29
43_L	130_I	0.981	0.29
261_K	288_D	0.978	0.29
142_S	281_K	0.977	0.29
177_S	235_T	0.972	0.29
239_A	311_A	0.972	0.29
62_S	100_I	0.966	0.29
262_W	288_D	0.965	0.29
62_S	103_K	0.965	0.29
71_T	78_L	0.964	0.29
280_V	284_A	0.963	0.29
112_E	150_I	0.962	0.29
48_P	69_S	0.955	0.28
27_D	273_S	0.954	0.28
96_A	148_I	0.952	0.28
144_E	198_N	0.951	0.28
34_L	148_I	0.950	0.28
45_H	80_T	0.949	0.28
226_R	274_L	0.947	0.28
78_L	170_Y	0.943	0.28
94_V	202_D	0.942	0.28
258_G	283_L	0.940	0.28
29_S	36_I	0.938	0.28
28_V	195_A	0.937	0.27
43_L	304_Y	0.934	0.27
27_D	30_L	0.934	0.27
181_P	218_V	0.933	0.27
222_S	332_I	0.932	0.27
292_S	333_D	0.930	0.27
68_V	71_T	0.926	0.27
109_L	130_I	0.925	0.27
257_I	339_L	0.924	0.27
244_V	255_I	0.918	0.27
20_I	24_G	0.917	0.27
38_G	51_D	0.912	0.26
48_P	116_D	0.912	0.26
207_L	275_A	0.911	0.26
132_E	322_G	0.909	0.26
230_L	279_G	0.908	0.26
276_K	337_S	0.906	0.26
187_L	271_M	0.905	0.26
171_D	200_P	0.904	0.26
48_P	215_L	0.902	0.26
155_P	177_S	0.901	0.26
121_A	226_R	0.900	0.26
71_T	122_L	0.898	0.26
18_A	92_I	0.894	0.26
154_I	289_L	0.892	0.26
163_A	263_I	0.892	0.26
95_N	111_G	0.890	0.26
128_N	324_S	0.890	0.26
86_F	132_E	0.889	0.26
140_E	255_I	0.885	0.25
81_R	178_S	0.881	0.25
162_M	166_L	0.877	0.25
162_M	192_V	0.870	0.25
270_D	277_Q	0.870	0.25
206_R	211_S	0.866	0.25
272_L	282_V	0.862	0.24
82_A	288_D	0.862	0.24
50_A	195_A	0.862	0.24
28_V	225_F	0.855	0.24
270_D	315_T	0.851	0.24
150_I	194_E	0.849	0.24
27_D	45_H	0.846	0.24
85_S	331_K	0.841	0.24
214_V	263_I	0.837	0.23
177_S	184_P	0.837	0.23
146_E	278_V	0.835	0.23
42_E	45_H	0.831	0.23
117_I	163_A	0.830	0.23
110_Q	324_S	0.828	0.23
96_A	141_L	0.828	0.23
32_P	261_K	0.827	0.23
121_A	328_L	0.823	0.23
132_E	135_I	0.820	0.23
164_T	199_L	0.815	0.23
165_L	263_I	0.815	0.23
138_G	151_G	0.813	0.23
146_E	286_M	0.812	0.23
24_G	94_V	0.811	0.23
297_I	333_D	0.811	0.23
92_I	216_L	0.809	0.22
21_L	39_A	0.807	0.22
228_K	284_A	0.806	0.22
73_I	336_Y	0.805	0.22
28_V	192_V	0.802	0.22
275_A	335_L	0.801	0.22
94_V	107_I	0.796	0.22
75_T	184_P	0.795	0.22
114_G	154_I	0.795	0.22
129_I	133_N	0.791	0.22
43_L	332_I	0.788	0.22
94_V	137_L	0.785	0.22
204_L	267_K	0.781	0.21
94_V	295_E	0.780	0.21
93_V	104_I	0.779	0.21
278_V	313_G	0.778	0.21
171_D	330_R	0.774	0.21
17_F	34_L	0.773	0.21
16_D	70_P	0.773	0.21
122_L	270_D	0.773	0.21
267_K	330_R	0.771	0.21
164_T	318_A	0.769	0.21
94_V	177_S	0.768	0.21
287_L	301_E	0.768	0.21
29_S	44_T	0.767	0.21
325_N	329_V	0.758	0.21
55_V	93_V	0.758	0.21
79_L	332_I	0.755	0.21
314_S	335_L	0.752	0.20
163_A	262_W	0.749	0.20
130_I	206_R	0.747	0.20
42_E	242_A	0.747	0.20
95_N	100_I	0.744	0.20
293_I	333_D	0.742	0.20
53_E	312_G	0.741	0.20
139_E	324_S	0.741	0.20
130_I	154_I	0.740	0.20
87_T	202_D	0.740	0.20
268_S	275_A	0.740	0.20
295_E	299_T	0.735	0.20
45_H	89_S	0.731	0.20
207_L	243_I	0.731	0.20
108_D	218_V	0.725	0.20
195_A	210_V	0.723	0.19
149_M	279_G	0.723	0.19
261_K	290_S	0.721	0.19
100_I	218_V	0.719	0.19
27_D	99_R	0.719	0.19
23_I	246_E	0.718	0.19
238_T	257_I	0.715	0.19
197_R	280_V	0.713	0.19
204_L	247_F	0.710	0.19
230_L	255_I	0.709	0.19
264_V	284_A	0.707	0.19
293_I	331_K	0.707	0.19
78_L	150_I	0.706	0.19
59_K	63_I	0.706	0.19
27_D	110_Q	0.704	0.19
29_S	98_S	0.703	0.19
248_D	252_L	0.702	0.19
58_G	81_R	0.701	0.19
232_A	263_I	0.698	0.19
194_E	327_T	0.696	0.19
104_I	144_E	0.696	0.19
21_L	25_T	0.694	0.19
59_K	336_Y	0.693	0.19
114_G	118_R	0.691	0.18
93_V	313_G	0.689	0.18
116_D	183_N	0.688	0.18
28_V	131_I	0.688	0.18
43_L	69_S	0.680	0.18
283_L	316_I	0.680	0.18
48_P	53_E	0.679	0.18
131_I	243_I	0.679	0.18
31_I	140_E	0.678	0.18
77_A	137_L	0.676	0.18
57_L	63_I	0.675	0.18
85_S	148_I	0.673	0.18
39_A	270_D	0.672	0.18
25_T	116_D	0.668	0.18
223_L	247_F	0.666	0.18
35_T	71_T	0.663	0.18
17_F	63_I	0.656	0.17
171_D	197_R	0.655	0.17
115_K	199_L	0.655	0.17
44_T	116_D	0.651	0.17
215_L	257_I	0.651	0.17
146_E	324_S	0.651	0.17
117_I	192_V	0.649	0.17
119_K	304_Y	0.649	0.17
38_G	47_T	0.649	0.17
47_T	51_D	0.649	0.17
95_N	203_P	0.649	0.17
130_I	209_K	0.648	0.17
279_G	324_S	0.644	0.17
178_S	336_Y	0.642	0.17
77_A	155_P	0.641	0.17
162_M	176_V	0.641	0.17
69_S	155_P	0.641	0.17
147_L	191_V	0.639	0.17
19_Y	269_A	0.639	0.17
182_D	293_I	0.637	0.17
70_P	248_D	0.635	0.17
19_Y	263_I	0.634	0.17
117_I	195_A	0.633	0.17
150_I	291_I	0.629	0.17
23_I	35_T	0.627	0.17
166_L	218_V	0.627	0.17
218_V	240_A	0.627	0.17
181_P	258_G	0.627	0.17
198_N	248_D	0.627	0.17
253_K	288_D	0.624	0.16
24_G	285_S	0.624	0.16
60_C	86_F	0.623	0.16
236_Q	306_K	0.622	0.16
102_P	237_M	0.621	0.16
164_T	216_L	0.620	0.16
50_A	160_T	0.620	0.16
47_T	53_E	0.619	0.16
222_S	239_A	0.618	0.16
17_F	35_T	0.617	0.16
193_E	207_L	0.616	0.16
197_R	227_G	0.615	0.16
58_G	154_I	0.615	0.16
40_T	305_V	0.614	0.16
70_P	155_P	0.612	0.16
39_A	181_P	0.611	0.16
28_V	163_A	0.610	0.16
285_S	323_V	0.609	0.16
132_E	326_S	0.609	0.16
119_K	319_F	0.608	0.16
21_L	241_A	0.606	0.16
296_G	336_Y	0.605	0.16
17_F	331_K	0.605	0.16
191_V	336_Y	0.603	0.16
69_S	73_I	0.603	0.16
81_R	159_T	0.603	0.16
83_S	109_L	0.602	0.16
166_L	192_V	0.600	0.16
49_A	315_T	0.599	0.16
48_P	75_T	0.599	0.16
143_N	247_F	0.598	0.16
107_I	256_I	0.596	0.16
184_P	224_G	0.595	0.16
132_E	191_V	0.594	0.16
214_V	311_A	0.593	0.16
121_A	134_G	0.590	0.15
102_P	214_V	0.589	0.15
195_A	262_W	0.588	0.15
17_F	332_I	0.588	0.15
69_S	296_G	0.586	0.15
167_A	315_T	0.586	0.15
281_K	299_T	0.586	0.15
70_P	314_S	0.585	0.15
180_S	187_L	0.584	0.15
109_L	217_G	0.583	0.15
181_P	235_T	0.583	0.15
149_M	281_K	0.583	0.15
32_P	318_A	0.583	0.15
104_I	214_V	0.583	0.15
57_L	270_D	0.580	0.15
81_R	113_P	0.578	0.15
57_L	150_I	0.578	0.15
102_P	160_T	0.576	0.15
87_T	314_S	0.575	0.15
37_A	51_D	0.574	0.15
243_I	271_M	0.574	0.15
170_Y	279_G	0.573	0.15
176_V	213_P	0.573	0.15
18_A	146_E	0.572	0.15
79_L	331_K	0.572	0.15
106_Y	149_M	0.572	0.15
49_A	336_Y	0.571	0.15
263_I	333_D	0.571	0.15
257_I	280_V	0.570	0.15
197_R	201_T	0.564	0.15
211_S	216_L	0.563	0.15
203_P	285_S	0.560	0.15
114_G	269_A	0.559	0.15
221_I	244_V	0.558	0.15
194_E	324_S	0.558	0.15
69_S	154_I	0.557	0.15
73_I	301_E	0.556	0.15
194_E	197_R	0.555	0.15
266_D	332_I	0.555	0.15
50_A	306_K	0.554	0.15
24_G	332_I	0.552	0.14
176_V	219_A	0.552	0.14
294_H	333_D	0.550	0.14
22_V	140_E	0.550	0.14
69_S	215_L	0.548	0.14
122_L	134_G	0.548	0.14
34_L	243_I	0.547	0.14
20_I	133_N	0.546	0.14
30_L	225_F	0.546	0.14
162_M	240_A	0.545	0.14
125_E	334_E	0.545	0.14
155_P	228_K	0.542	0.14
56_I	105_P	0.541	0.14
104_I	320_I	0.541	0.14
154_I	274_L	0.540	0.14
26_T	271_M	0.540	0.14
50_A	62_S	0.536	0.14
146_E	195_A	0.536	0.14
133_N	203_P	0.536	0.14
122_L	199_L	0.536	0.14
136_K	190_K	0.535	0.14
79_L	287_L	0.535	0.14
116_D	130_I	0.534	0.14
134_G	153_S	0.533	0.14
59_K	64_N	0.530	0.14
126_K	129_I	0.530	0.14
113_P	267_K	0.528	0.14
250_S	267_K	0.526	0.14
273_S	315_T	0.525	0.14
132_E	186_E	0.524	0.14
328_L	338_E	0.523	0.14
22_V	221_I	0.522	0.14
44_T	118_R	0.521	0.14
279_G	330_R	0.521	0.14
104_I	246_E	0.520	0.14
95_N	271_M	0.519	0.14
34_L	126_K	0.518	0.14
18_A	148_I	0.517	0.14
52_A	55_V	0.515	0.14
128_N	204_L	0.515	0.14
92_I	115_K	0.514	0.14
54_Y	335_L	0.512	0.13
50_A	229_I	0.511	0.13
149_M	309_V	0.511	0.13
23_I	40_T	0.510	0.13
194_E	268_S	0.508	0.13
120_Q	136_K	0.508	0.13
262_W	289_L	0.507	0.13
255_I	298_R	0.506	0.13
186_E	197_R	0.506	0.13
71_T	116_D	0.505	0.13
17_F	147_L	0.502	0.13
85_S	338_E	0.501	0.13
100_I	313_G	0.500	0.13

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3u4gA	2	0.9193	100	0.335	Contact Map
3l0zA	2	0.9568	100	0.373	Contact Map
4hdrA	1	0.8012	100	0.757	Contact Map
1wx1A	2	0.8242	100	0.761	Contact Map
4kqiA	2	0.8156	100	0.761	Contact Map
4hdrB	1	0.8127	100	0.772	Contact Map
2r62A	1	0.6282	24.7	0.976	Contact Map
2e0nA	2	0.6052	15.7	0.979	Contact Map
2an7A	2	0.1354	14.8	0.979	Contact Map
3eieA	1	0.6398	14.7	0.979	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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