Q97W87 - SULSO - GREMLIN Contacts

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Q97W87 - Asparinase, putative (AnsA-like)

UniProt:	Q97W87	Sequences: MRYNLPVLVVHGGAGSWQIADQDKAKLTISEALERGYYEFRKGSAIEAVVEAIYYMEESGVFDAGKGSVRNSAGYIEMDAGIMVGNTLQVGGIMGLREGSAIKKALEILFQNRHVLMIGSNYNSSNSNNSAIYESKVSGDTVGAVALDQHGNLVAGTSTGGIKGKLPGRVGDSPIPGAGYYATSNVAVSSTGIGEIILRILPAKEVDILVSLGFTIDDALRAVVNKVTKTFGKDNIGMIGLDKYGNASAYYNTKGMARGVISSDGVKKVYVFEGDI	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	276 (257)
Sequences:	2070 (1237)
Seq/√Len:	77.1

ECOLI - IAAA
HALSA - Q9HNZ8
SULSO - Q97W87

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
50_V	103_K	4.598	1.00
53_I	156_G	4.337	1.00
242_D	246_N	4.255	1.00
152_N	184_S	3.805	1.00
153_L	187_A	3.542	1.00
54_Y	103_K	3.503	1.00
58_E	100_S	3.466	1.00
102_I	156_G	3.224	1.00
96_L	104_K	3.187	1.00
68_S	78_M	3.123	1.00
206_V	238_M	3.046	1.00
47_E	106_L	2.780	1.00
217_D	221_R	2.676	1.00
94_M	118_I	2.660	1.00
215_T	218_D	2.653	1.00
118_I	170_V	2.581	1.00
28_T	59_S	2.555	1.00
106_L	110_F	2.533	1.00
70_R	76_I	2.504	1.00
209_L	214_F	2.407	1.00
75_Y	97_R	2.391	1.00
33_L	259_G	2.386	1.00
201_L	204_K	2.371	1.00
71_N	75_Y	2.269	1.00
117_M	125_S	2.179	1.00
49_V	146_A	2.116	1.00
71_N	77_E	2.115	1.00
50_V	106_L	2.007	1.00
84_V	87_T	1.965	1.00
82_I	102_I	1.951	1.00
39_E	43_G	1.877	1.00
221_R	225_N	1.850	1.00
104_K	131_A	1.778	1.00
50_V	82_I	1.762	1.00
214_F	222_A	1.693	1.00
88_L	181_Y	1.661	1.00
147_L	241_L	1.658	1.00
92_G	116_L	1.646	1.00
205_E	208_I	1.645	1.00
25_A	61_V	1.634	1.00
175_I	178_A	1.630	1.00
81_G	180_Y	1.621	1.00
107_E	111_Q	1.620	1.00
73_A	124_S	1.613	1.00
188_V	240_G	1.605	1.00
202_P	223_V	1.605	1.00
216_I	240_G	1.601	1.00
48_A	52_A	1.596	1.00
120_S	124_S	1.593	1.00
90_V	176_P	1.580	0.99
157_T	189_S	1.575	0.99
90_V	115_V	1.541	0.99
125_S	129_N	1.530	0.99
197_I	236_I	1.508	0.99
95_G	118_I	1.499	0.99
98_E	123_N	1.499	0.99
220_L	240_G	1.488	0.99
209_L	222_A	1.478	0.99
53_I	102_I	1.477	0.99
262_S	265_G	1.464	0.99
23_D	27_L	1.463	0.99
224_V	252_N	1.461	0.99
39_E	48_A	1.455	0.99
32_A	55_Y	1.454	0.99
53_I	142_V	1.434	0.99
35_R	55_Y	1.430	0.99
122_Y	126_N	1.406	0.99
52_A	144_A	1.402	0.99
8_L	33_L	1.380	0.99
35_R	38_Y	1.371	0.99
22_Q	26_K	1.365	0.98
147_L	153_L	1.359	0.98
86_N	184_S	1.346	0.98
162_I	193_I	1.336	0.98
58_E	99_G	1.313	0.98
50_V	54_Y	1.311	0.98
204_K	208_I	1.310	0.98
205_E	212_L	1.302	0.98
153_L	182_A	1.300	0.98
188_V	203_A	1.293	0.98
247_A	260_V	1.288	0.98
49_V	156_G	1.288	0.98
107_E	129_N	1.280	0.98
84_V	89_Q	1.269	0.98
94_M	170_V	1.266	0.98
31_E	59_S	1.249	0.97
175_I	198_L	1.236	0.97
103_K	106_L	1.215	0.97
183_T	211_S	1.202	0.97
112_N	166_L	1.191	0.96
108_I	112_N	1.191	0.96
235_N	253_T	1.178	0.96
189_S	239_I	1.175	0.96
145_V	187_A	1.171	0.96
32_A	36_G	1.170	0.96
190_S	238_M	1.163	0.96
45_A	146_A	1.148	0.96
111_Q	129_N	1.148	0.96
197_I	202_P	1.146	0.96
77_E	97_R	1.131	0.95
228_T	231_F	1.129	0.95
155_A	180_Y	1.126	0.95
34_E	261_I	1.126	0.95
36_G	52_A	1.120	0.95
84_V	152_N	1.107	0.95
208_I	211_S	1.107	0.95
100_S	103_K	1.078	0.94
45_A	154_V	1.066	0.93
206_V	240_G	1.062	0.93
107_E	131_A	1.052	0.93
78_M	96_L	1.051	0.93
51_E	54_Y	1.048	0.93
37_Y	41_R	1.044	0.93
54_Y	100_S	1.043	0.93
202_P	227_V	1.042	0.93
30_S	34_E	1.030	0.92
116_L	175_I	1.030	0.92
191_T	256_M	1.023	0.92
186_V	207_D	1.023	0.92
86_N	152_N	1.009	0.91
28_T	32_A	1.007	0.91
43_G	47_E	1.000	0.91
8_L	261_I	1.000	0.91
109_L	112_N	0.995	0.91
123_N	127_S	0.990	0.91
10_V	32_A	0.988	0.90
119_G	126_N	0.986	0.90
32_A	51_E	0.983	0.90
35_R	51_E	0.980	0.90
28_T	31_E	0.977	0.90
37_Y	261_I	0.966	0.89
112_N	125_S	0.964	0.89
18_Q	21_D	0.954	0.89
72_S	167_P	0.949	0.89
63_D	69_V	0.946	0.88
221_R	250_Y	0.944	0.88
46_I	82_I	0.929	0.88
222_A	226_K	0.928	0.88
85_G	182_A	0.927	0.87
210_V	240_G	0.911	0.87
108_I	125_S	0.910	0.86
38_Y	41_R	0.909	0.86
190_S	203_A	0.905	0.86
159_T	173_S	0.902	0.86
205_E	209_L	0.901	0.86
9_V	187_A	0.900	0.86
143_G	189_S	0.893	0.85
208_I	212_L	0.887	0.85
214_F	218_D	0.886	0.85
38_Y	42_K	0.877	0.84
50_V	110_F	0.873	0.84
115_V	195_E	0.869	0.84
8_L	36_G	0.867	0.84
227_V	230_T	0.862	0.83
35_R	52_A	0.862	0.83
176_P	180_Y	0.862	0.83
91_G	108_I	0.861	0.83
159_T	172_D	0.859	0.83
121_N	125_S	0.850	0.82
210_V	242_D	0.844	0.82
145_V	189_S	0.844	0.82
81_G	201_L	0.842	0.82
53_I	144_A	0.838	0.82
171_G	174_P	0.834	0.81
15_G	63_D	0.831	0.81
32_A	48_A	0.828	0.81
49_V	154_V	0.828	0.81
182_A	187_A	0.827	0.81
69_V	165_K	0.824	0.80
148_D	152_N	0.822	0.80
144_A	156_G	0.820	0.80
45_A	148_D	0.816	0.80
46_I	154_V	0.811	0.79
46_I	110_F	0.809	0.79
10_V	259_G	0.809	0.79
94_M	116_L	0.809	0.79
40_F	48_A	0.804	0.79
188_V	207_D	0.799	0.78
104_K	125_S	0.788	0.77
93_I	108_I	0.785	0.77
81_G	176_P	0.785	0.77
51_E	55_Y	0.785	0.77
56_M	64_A	0.782	0.77
251_Y	256_M	0.781	0.77
220_L	238_M	0.776	0.76
157_T	239_I	0.775	0.76
39_E	51_E	0.771	0.76
10_V	29_I	0.765	0.75
61_V	197_I	0.760	0.75
54_Y	58_E	0.758	0.75
39_E	42_K	0.750	0.74
64_A	142_V	0.746	0.74
140_D	189_S	0.744	0.73
90_V	180_Y	0.742	0.73
157_T	180_Y	0.740	0.73
153_L	242_D	0.737	0.73
68_S	76_I	0.733	0.72
78_M	123_N	0.732	0.72
200_I	231_F	0.731	0.72
15_G	25_A	0.730	0.72
122_Y	125_S	0.730	0.72
124_S	128_N	0.729	0.72
155_A	182_A	0.727	0.72
46_I	50_V	0.725	0.71
73_A	167_P	0.723	0.71
40_F	43_G	0.721	0.71
44_S	150_H	0.715	0.70
28_T	62_F	0.715	0.70
68_S	160_G	0.714	0.70
238_M	250_Y	0.714	0.70
77_E	165_K	0.714	0.70
12_G	62_F	0.714	0.70
115_V	198_L	0.713	0.70
16_S	257_A	0.711	0.70
190_S	206_V	0.710	0.70
261_I	265_G	0.710	0.70
178_A	201_L	0.710	0.70
216_I	248_S	0.708	0.70
97_R	120_S	0.707	0.69
60_G	66_K	0.704	0.69
108_I	114_H	0.702	0.69
63_D	70_R	0.701	0.69
90_V	116_L	0.699	0.69
136_K	139_G	0.697	0.68
140_D	180_Y	0.690	0.68
35_R	39_E	0.685	0.67
55_Y	59_S	0.683	0.67
21_D	24_K	0.682	0.67
183_T	243_K	0.680	0.66
251_Y	258_R	0.679	0.66
48_A	51_E	0.677	0.66
44_S	47_E	0.676	0.66
70_R	162_I	0.670	0.65
63_D	160_G	0.669	0.65
174_P	190_S	0.669	0.65
91_G	105_A	0.668	0.65
23_D	30_S	0.666	0.65
79_D	121_N	0.665	0.65
100_S	132_I	0.664	0.65
115_V	176_P	0.662	0.64
200_I	204_K	0.658	0.64
83_M	90_V	0.657	0.64
227_V	231_F	0.654	0.63
15_G	165_K	0.654	0.63
79_D	170_V	0.653	0.63
202_P	238_M	0.653	0.63
55_Y	58_E	0.650	0.63
210_V	213_G	0.647	0.63
17_W	255_G	0.647	0.63
204_K	209_L	0.640	0.62
56_M	160_G	0.640	0.62
69_V	163_K	0.640	0.62
224_V	227_V	0.636	0.61
218_D	222_A	0.635	0.61
79_D	173_S	0.635	0.61
213_G	219_A	0.634	0.61
8_L	146_A	0.633	0.61
237_G	251_Y	0.632	0.61
255_G	258_R	0.631	0.61
218_D	221_R	0.631	0.61
142_V	158_S	0.631	0.61
19_I	22_Q	0.630	0.61
157_T	173_S	0.629	0.60
25_A	29_I	0.628	0.60
225_N	228_T	0.628	0.60
36_G	146_A	0.626	0.60
214_F	219_A	0.624	0.60
104_K	107_E	0.624	0.60
15_G	255_G	0.623	0.60
208_I	226_K	0.623	0.60
107_E	110_F	0.620	0.59
220_L	250_Y	0.619	0.59
18_Q	254_K	0.619	0.59
104_K	126_N	0.618	0.59
237_G	256_M	0.617	0.59
259_G	263_S	0.609	0.58
126_N	130_S	0.608	0.58
66_K	100_S	0.607	0.58
81_G	155_A	0.606	0.58
18_Q	210_V	0.606	0.58
46_I	109_L	0.605	0.57
78_M	97_R	0.604	0.57
185_N	243_K	0.604	0.57
260_V	265_G	0.604	0.57
253_T	256_M	0.603	0.57
183_T	186_V	0.603	0.57
196_I	200_I	0.601	0.57
71_N	165_K	0.601	0.57
169_R	194_G	0.600	0.57
220_L	224_V	0.597	0.56
48_A	146_A	0.595	0.56
219_A	223_V	0.591	0.56
31_E	34_E	0.591	0.56
123_N	131_A	0.591	0.56
161_G	169_R	0.591	0.56
121_N	195_E	0.589	0.55
16_S	254_K	0.587	0.55
44_S	152_N	0.586	0.55
106_L	109_L	0.586	0.55
31_E	38_Y	0.586	0.55
217_D	250_Y	0.586	0.55
153_L	178_A	0.585	0.55
225_N	231_F	0.583	0.55
81_G	101_A	0.583	0.55
66_K	76_I	0.582	0.55
256_M	259_G	0.577	0.54
120_S	123_N	0.576	0.54
38_Y	51_E	0.575	0.54
147_L	151_G	0.574	0.54
79_D	91_G	0.573	0.53
249_A	258_R	0.573	0.53
205_E	226_K	0.572	0.53
217_D	248_S	0.565	0.52
29_I	33_L	0.565	0.52
58_E	101_A	0.563	0.52
81_G	90_V	0.563	0.52
141_T	194_G	0.563	0.52
141_T	169_R	0.562	0.52
224_V	228_T	0.560	0.52
209_L	212_L	0.559	0.52
234_D	252_N	0.559	0.52
62_F	69_V	0.558	0.52
204_K	211_S	0.554	0.51
31_E	35_R	0.554	0.51
33_L	261_I	0.554	0.51
185_N	211_S	0.551	0.51
224_V	236_I	0.551	0.51
161_G	168_G	0.548	0.50
209_L	227_V	0.548	0.50
124_S	127_S	0.547	0.50
95_G	121_N	0.546	0.50
28_T	55_Y	0.546	0.50
9_V	247_A	0.542	0.50
41_R	261_I	0.540	0.49
99_G	132_I	0.540	0.49
140_D	256_M	0.540	0.49
119_G	122_Y	0.540	0.49
171_G	194_G	0.540	0.49
143_G	158_S	0.537	0.49
62_F	157_T	0.536	0.49
34_E	42_K	0.536	0.49
153_L	246_N	0.536	0.49
16_S	255_G	0.534	0.49
188_V	238_M	0.533	0.48
54_Y	156_G	0.530	0.48
157_T	172_D	0.529	0.48
99_G	124_S	0.528	0.48
10_V	52_A	0.528	0.48
200_I	227_V	0.527	0.48
47_E	50_V	0.527	0.48
64_A	160_G	0.527	0.48
17_W	257_A	0.526	0.47
185_N	207_D	0.524	0.47
133_Y	136_K	0.524	0.47
157_T	191_T	0.523	0.47
214_F	217_D	0.522	0.47
29_I	258_R	0.520	0.47
144_A	256_M	0.520	0.47
147_L	223_V	0.519	0.47
161_G	165_K	0.519	0.47
12_G	245_G	0.516	0.46
131_A	185_N	0.515	0.46
52_A	146_A	0.515	0.46
35_R	243_K	0.514	0.46
82_I	156_G	0.513	0.46
20_A	25_A	0.513	0.46
48_A	55_Y	0.512	0.46
164_G	207_D	0.512	0.46
144_A	187_A	0.511	0.46
32_A	35_R	0.511	0.46
39_E	146_A	0.508	0.45
150_H	244_Y	0.508	0.45
143_G	160_G	0.507	0.45
96_L	101_A	0.507	0.45
209_L	213_G	0.507	0.45
90_V	112_N	0.506	0.45
209_L	226_K	0.506	0.45
69_V	160_G	0.505	0.45
43_G	48_A	0.505	0.45
30_S	42_K	0.504	0.45
174_P	199_R	0.504	0.45
128_N	166_L	0.503	0.45
17_W	20_A	0.503	0.45
151_G	154_V	0.501	0.44
215_T	219_A	0.501	0.44
216_I	224_V	0.500	0.44

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3c17A	2	0.9819	100	0.096	Contact Map
4o0cA	2	0.9746	100	0.103	Contact Map
2a8jA	2	0.9638	100	0.127	Contact Map
1p4kA	2	0.942	100	0.216	Contact Map
4pv2A	1	0.4964	100	0.445	Contact Map
1k2xA	1	0.4964	100	0.547	Contact Map
2gezA	2	0.5761	100	0.56	Contact Map
1k2xB	2	0.4819	100	0.574	Contact Map
4pv2B	2	0.471	100	0.579	Contact Map
2gezB	2	0.4783	100	0.58	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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