Q97W53 - SULSO - GREMLIN Contacts

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Q97W53 - Type II secretion system protein, virB homolog (GspE-3)

UniProt:	Q97W53	Sequences: MGEWYIMSKIFKFPLQIGRGSVKQLPITDLPITLYPVTPLPEEVTTIVADYEVNILNLVPEDIKSNLTRNNIELILPNPHVFITFDERKGIYKYVLLEPPVNEMIYNIYNIFIEEVERELLSKNPSLDLAKIIFELDKKRSGLKIIQEKRGDIYVLSTNARVTLYYLLRNMFGYNVLTPLVADKNIEDISVPGLNNPVYVYHRSYEYIPTNIIFTKNMQVSPQLNIMIDGEELLDQLVLRMLSTTGKSISVAEPIQDGMLPNGDRVAATFSGRPKITSIKINL	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	283 (229)
Sequences:	241 (198)
Seq/√Len:	13.1

SULSO - Q97W53

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
96_L	211_N	2.791	0.95
212_I	274_P	2.763	0.95
116_V	258_G	2.598	0.93
165_Y	250_S	2.550	0.92
270_F	280_K	2.361	0.88
73_E	205_Y	2.264	0.86
200_V	212_I	2.237	0.85
242_L	280_K	2.237	0.85
94_Y	187_E	2.232	0.85
53_V	59_V	2.211	0.85
73_E	199_Y	2.198	0.84
248_S	273_R	2.192	0.84
135_E	168_L	2.177	0.84
194_L	235_D	2.090	0.81
51_Y	60_P	2.087	0.81
77_P	178_T	2.057	0.80
140_R	232_E	2.044	0.79
241_M	247_K	1.988	0.78
174_Y	178_T	1.985	0.77
94_Y	202_H	1.971	0.77
187_E	202_H	1.951	0.76
165_Y	169_R	1.949	0.76
243_S	267_A	1.901	0.74
267_A	276_I	1.884	0.73
191_V	279_I	1.864	0.73
59_V	241_M	1.856	0.72
165_Y	188_D	1.828	0.71
138_K	215_T	1.799	0.70
189_I	212_I	1.783	0.69
191_V	281_I	1.768	0.68
105_I	162_V	1.690	0.65
50_D	180_L	1.656	0.63
206_E	247_K	1.647	0.63
79_P	188_D	1.629	0.62
257_D	266_V	1.594	0.60
177_L	237_L	1.568	0.59
188_D	250_S	1.564	0.59
180_L	212_I	1.564	0.59
169_R	250_S	1.559	0.58
93_K	208_I	1.557	0.58
77_P	81_V	1.555	0.58
40_L	208_I	1.539	0.57
190_S	270_F	1.538	0.57
270_F	275_K	1.535	0.57
82_F	97_L	1.532	0.57
192_P	279_I	1.529	0.57
188_D	243_S	1.522	0.56
250_S	273_R	1.517	0.56
142_G	175_N	1.515	0.56
205_Y	275_K	1.505	0.56
112_F	140_R	1.504	0.56
247_K	267_A	1.503	0.55
268_A	280_K	1.495	0.55
190_S	207_Y	1.492	0.55
173_G	246_G	1.490	0.55
257_D	267_A	1.479	0.54
251_V	273_R	1.464	0.53
257_D	269_T	1.463	0.53
98_E	165_Y	1.454	0.53
207_Y	267_A	1.443	0.52
245_T	274_P	1.432	0.52
72_I	85_F	1.418	0.51
190_S	271_S	1.403	0.50
55_I	58_L	1.397	0.50
98_E	188_D	1.393	0.50
43_E	46_T	1.382	0.49
238_V	279_I	1.364	0.48
43_E	47_I	1.359	0.48
181_V	237_L	1.357	0.48
191_V	238_V	1.354	0.48
133_I	136_L	1.322	0.46
76_L	262_N	1.316	0.46
51_Y	56_L	1.306	0.45
95_V	177_L	1.303	0.45
45_T	48_V	1.301	0.45
257_D	280_K	1.295	0.45
264_D	267_A	1.288	0.44
239_L	271_S	1.283	0.44
278_S	283_L	1.282	0.44
120_L	161_R	1.276	0.44
259_M	271_S	1.265	0.43
176_V	214_F	1.257	0.43
192_P	207_Y	1.256	0.43
108_I	159_N	1.251	0.42
159_N	241_M	1.250	0.42
238_V	249_I	1.248	0.42
99_P	263_G	1.247	0.42
113_I	170_N	1.228	0.41
190_S	250_S	1.220	0.41
191_V	234_L	1.213	0.40
244_T	257_D	1.188	0.39
190_S	281_I	1.185	0.39
130_A	133_I	1.176	0.39
186_I	200_V	1.174	0.38
99_P	243_S	1.173	0.38
66_N	155_V	1.172	0.38
259_M	267_A	1.168	0.38
37_V	41_P	1.158	0.38
165_Y	190_S	1.155	0.38
57_N	60_P	1.151	0.37
261_P	282_N	1.145	0.37
249_I	255_I	1.131	0.36
51_Y	57_N	1.121	0.36
122_S	280_K	1.115	0.36
201_Y	243_S	1.095	0.35
208_I	277_T	1.088	0.34
243_S	271_S	1.087	0.34
193_G	202_H	1.087	0.34
250_S	259_M	1.086	0.34
243_S	248_S	1.077	0.34
40_L	50_D	1.074	0.34
99_P	165_Y	1.072	0.34
193_G	234_L	1.065	0.33
36_P	39_P	1.064	0.33
76_L	182_A	1.045	0.32
190_S	241_M	1.041	0.32
120_L	180_L	1.040	0.32
138_K	149_K	1.021	0.31
205_Y	245_T	1.018	0.31
74_L	79_P	1.017	0.31
194_L	255_I	1.015	0.31
173_G	189_I	1.015	0.31
210_T	246_G	1.014	0.31
181_V	244_T	1.012	0.31
146_I	259_M	1.009	0.31
136_L	198_V	1.009	0.31
90_G	256_Q	1.008	0.31
73_E	212_I	1.000	0.30
182_A	255_I	0.999	0.30
130_A	189_I	0.994	0.30
53_V	56_L	0.989	0.30
241_M	246_G	0.989	0.30
99_P	264_D	0.989	0.30
169_R	188_D	0.989	0.30
240_R	258_G	0.984	0.30
116_V	163_T	0.983	0.30
239_L	250_S	0.976	0.29
191_V	273_R	0.971	0.29
39_P	45_T	0.969	0.29
70_N	87_E	0.961	0.29
206_E	238_V	0.952	0.28
98_E	169_R	0.948	0.28
110_N	249_I	0.948	0.28
71_N	201_Y	0.945	0.28
103_E	130_A	0.933	0.27
166_Y	169_R	0.928	0.27
76_L	95_V	0.925	0.27
36_P	41_P	0.924	0.27
182_A	244_T	0.920	0.27
133_I	212_I	0.907	0.26
62_D	189_I	0.906	0.26
274_P	282_N	0.903	0.26
57_N	92_Y	0.898	0.26
39_P	274_P	0.896	0.26
54_N	97_L	0.895	0.26
94_Y	99_P	0.892	0.26
243_S	259_M	0.890	0.26
148_E	153_I	0.890	0.26
252_A	266_V	0.889	0.26
160_A	252_A	0.887	0.26
51_Y	259_M	0.884	0.25
39_P	44_V	0.884	0.25
178_T	243_S	0.883	0.25
182_A	274_P	0.882	0.25
183_D	254_P	0.877	0.25
183_D	265_R	0.877	0.25
254_P	265_R	0.877	0.25
211_N	269_T	0.876	0.25
95_V	215_T	0.875	0.25
199_Y	242_L	0.874	0.25
180_L	277_T	0.866	0.25
165_Y	281_I	0.865	0.25
56_L	60_P	0.865	0.25
74_L	188_D	0.863	0.25
90_G	148_E	0.862	0.25
249_I	268_A	0.856	0.24
102_N	130_A	0.855	0.24
252_A	281_I	0.851	0.24
146_I	194_L	0.842	0.24
172_F	257_D	0.840	0.24
53_V	58_L	0.833	0.23
191_V	237_L	0.829	0.23
102_N	105_I	0.828	0.23
98_E	250_S	0.827	0.23
50_D	104_M	0.827	0.23
198_V	274_P	0.824	0.23
184_K	277_T	0.823	0.23
113_I	161_R	0.823	0.23
54_N	100_P	0.821	0.23
77_P	273_R	0.821	0.23
180_L	251_V	0.817	0.23
96_L	179_P	0.817	0.23
57_N	159_N	0.813	0.23
195_N	215_T	0.812	0.23
103_E	133_I	0.810	0.23
180_L	186_I	0.809	0.23
241_M	255_I	0.804	0.22
247_K	258_G	0.794	0.22
127_L	144_K	0.790	0.22
147_Q	155_V	0.790	0.22
47_I	76_L	0.783	0.22
144_K	232_E	0.781	0.22
79_P	250_S	0.773	0.21
196_N	277_T	0.772	0.21
129_L	212_I	0.769	0.21
46_T	50_D	0.765	0.21
54_N	155_V	0.757	0.21
93_K	115_E	0.755	0.21
123_K	177_L	0.754	0.21
125_P	174_Y	0.752	0.21
119_E	163_T	0.750	0.20
96_L	233_L	0.746	0.20
71_N	82_F	0.744	0.20
67_L	100_P	0.741	0.20
64_K	203_R	0.739	0.20
79_P	193_G	0.737	0.20
242_L	269_T	0.736	0.20
86_D	258_G	0.732	0.20
258_G	283_L	0.728	0.20
51_Y	189_I	0.727	0.20
104_M	199_Y	0.726	0.20
75_I	117_E	0.726	0.20
42_E	46_T	0.726	0.20
248_S	275_K	0.725	0.20
133_I	205_Y	0.720	0.19
243_S	270_F	0.717	0.19
88_R	144_K	0.715	0.19
234_L	281_I	0.711	0.19
255_I	275_K	0.708	0.19
194_L	200_V	0.707	0.19
176_V	270_F	0.700	0.19
145_I	175_N	0.698	0.19
146_I	199_Y	0.698	0.19
95_V	208_I	0.695	0.19
94_Y	261_P	0.692	0.19
177_L	261_P	0.691	0.19
77_P	149_K	0.686	0.18
50_D	60_P	0.679	0.18
66_N	167_L	0.679	0.18
112_F	214_F	0.675	0.18
161_R	177_L	0.675	0.18
72_I	140_R	0.675	0.18
63_I	68_T	0.672	0.18
88_R	147_Q	0.671	0.18
96_L	236_Q	0.670	0.18
91_I	113_I	0.668	0.18
67_L	72_I	0.667	0.18
190_S	267_A	0.664	0.18
252_A	280_K	0.662	0.18
178_T	251_V	0.662	0.18
81_V	273_R	0.658	0.17
36_P	156_L	0.655	0.17
128_D	231_E	0.654	0.17
165_Y	271_S	0.654	0.17
181_V	200_V	0.653	0.17
255_I	258_G	0.651	0.17
139_K	168_L	0.650	0.17
67_L	88_R	0.649	0.17
259_M	270_F	0.649	0.17
249_I	256_Q	0.648	0.17
159_N	167_L	0.647	0.17
116_V	235_D	0.645	0.17
63_I	241_M	0.645	0.17
101_V	107_N	0.641	0.17
250_S	277_T	0.641	0.17
117_E	176_V	0.638	0.17
75_I	171_M	0.637	0.17
96_L	130_A	0.636	0.17
165_Y	270_F	0.634	0.17
185_N	203_R	0.628	0.17
100_P	177_L	0.628	0.17
239_L	256_Q	0.626	0.17
79_P	205_Y	0.617	0.16
173_G	280_K	0.615	0.16
132_I	153_I	0.612	0.16
188_D	281_I	0.610	0.16
255_I	276_I	0.610	0.16
141_S	200_V	0.608	0.16
98_E	273_R	0.608	0.16
259_M	262_N	0.608	0.16
131_K	141_S	0.604	0.16
179_P	213_I	0.602	0.16
213_I	233_L	0.600	0.16
105_I	243_S	0.599	0.16
55_I	235_D	0.598	0.16
199_Y	255_I	0.598	0.16
146_I	274_P	0.598	0.16
190_S	247_K	0.591	0.16
248_S	271_S	0.586	0.15
250_S	271_S	0.583	0.15
116_V	196_N	0.582	0.15
190_S	239_L	0.582	0.15
148_E	255_I	0.582	0.15
237_L	250_S	0.581	0.15
72_I	249_I	0.579	0.15
80_H	83_I	0.579	0.15
173_G	178_T	0.578	0.15
99_P	271_S	0.577	0.15
243_S	264_D	0.576	0.15
171_M	243_S	0.576	0.15
254_P	278_S	0.574	0.15
265_R	278_S	0.574	0.15
183_D	278_S	0.574	0.15
75_I	82_F	0.573	0.15
111_I	115_E	0.563	0.15
158_T	164_L	0.562	0.15
192_P	275_K	0.561	0.15
262_N	267_A	0.560	0.15
52_E	90_G	0.553	0.15
184_K	249_I	0.553	0.15
136_L	235_D	0.551	0.14
269_T	280_K	0.547	0.14
41_P	44_V	0.546	0.14
107_N	111_I	0.546	0.14
108_I	191_V	0.543	0.14
137_D	178_T	0.542	0.14
45_T	212_I	0.537	0.14
169_R	190_S	0.537	0.14
42_E	135_E	0.536	0.14
185_N	269_T	0.535	0.14
86_D	192_P	0.529	0.14
114_E	155_V	0.528	0.14
44_V	114_E	0.526	0.14
119_E	267_A	0.526	0.14
101_V	281_I	0.524	0.14
68_T	213_I	0.524	0.14
110_N	180_L	0.523	0.14
96_L	144_K	0.523	0.14
111_I	134_F	0.522	0.14
48_V	172_F	0.522	0.14
92_Y	198_V	0.519	0.14
62_D	232_E	0.517	0.14
62_D	198_V	0.514	0.14
99_P	187_E	0.511	0.13
242_L	282_N	0.510	0.13
120_L	138_K	0.509	0.13
239_L	243_S	0.504	0.13
75_I	79_P	0.504	0.13
87_E	114_E	0.503	0.13
60_P	172_F	0.503	0.13
187_E	261_P	0.501	0.13
139_K	208_I	0.500	0.13
177_L	200_V	0.500	0.13
44_V	62_D	0.500	0.13

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2oap1	3	0.7668	100	0.572	Contact Map
4ihqA	3	0.7703	100	0.586	Contact Map
2gzaA	3	0.3781	99.7	0.807	Contact Map
2pt7A	3	0.4276	97.7	0.912	Contact Map
2ewvA	3	0.3392	96.4	0.926	Contact Map
3jvvA	3	0.3286	94.9	0.934	Contact Map
4chmA	1	0.1802	25.9	0.966	Contact Map
1owfA	1	0.159	16.3	0.969	Contact Map
1lyqA	1	0.1378	15.4	0.97	Contact Map
2akkA	1	0.1095	15.1	0.97	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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