GREMLIN Database
PANB - 3-methyl-2-oxobutanoate hydroxymethyltransferase
UniProt: Q97W44 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 267 (261)
Sequences: 3105 (1879)
Seq/√Len: 116.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
18_K254_N3.4181.00
88_L102_G3.0911.00
235_N238_E2.9181.00
190_K194_E2.8831.00
124_I171_L2.8151.00
69_T83_S2.7791.00
190_K208_Y2.6581.00
233_Y250_D2.6301.00
21_M213_V2.5911.00
62_M100_N2.5791.00
172_E197_N2.4161.00
169_L195_K2.3121.00
13_K210_D2.2651.00
48_G60_V2.2631.00
172_E196_L2.1611.00
96_S99_K2.1461.00
62_M89_S2.1351.00
52_L67_S2.1251.00
135_M178_S2.0991.00
17_E252_K2.0651.00
239_I242_S2.0631.00
231_K250_D2.0481.00
231_K261_H2.0161.00
12_K19_I2.0161.00
20_T248_I2.0021.00
193_T200_T1.9731.00
38_L248_I1.9531.00
92_I99_K1.9461.00
162_E166_K1.9201.00
248_I252_K1.9041.00
11_K15_M1.9031.00
125_R129_K1.8601.00
118_E166_K1.8251.00
191_R195_K1.8201.00
64_D70_R1.8111.00
73_A81_I1.7731.00
249_I253_T1.7731.00
186_V262_Y1.7581.00
234_V239_I1.7511.00
135_M177_F1.7241.00
242_S246_Q1.7091.00
62_M104_L1.7031.00
69_T109_S1.6781.00
33_I41_I1.6711.00
83_S104_L1.6471.00
163_Q167_D1.6431.00
33_I244_I1.6401.00
157_T160_E1.6361.00
85_M100_N1.6141.00
34_S79_Q1.6021.00
56_N59_N1.5611.00
136_G171_L1.5601.00
52_L71_A1.5211.00
9_F40_S1.5111.00
176_V198_V1.5111.00
207_P262_Y1.5101.00
41_I76_N1.5051.00
226_T232_S1.5021.00
165_I191_R1.4981.00
258_E261_H1.4961.00
40_S213_V1.4931.00
29_T216_I1.4691.00
169_L172_E1.4661.00
63_R103_L1.4621.00
154_I160_E1.4601.00
250_D255_K1.4471.00
97_A123_I1.4461.00
12_K17_E1.4451.00
69_T107_A1.4401.00
232_S235_N1.4171.00
238_E242_S1.4121.00
104_L107_A1.3931.00
136_G176_V1.3911.00
85_M101_A1.3621.00
111_A177_F1.3591.00
172_E198_V1.3401.00
40_S135_M1.3171.00
21_M40_S1.3171.00
179_I196_L1.3061.00
171_L176_V1.3031.00
92_I96_S1.2861.00
220_L240_I1.2750.99
83_S107_A1.2600.99
94_V123_I1.2570.99
141_T148_L1.2540.99
35_N241_S1.2510.99
169_L196_L1.2500.99
126_A130_A1.2490.99
66_I104_L1.2490.99
158_K162_E1.2480.99
138_I181_I1.2440.99
138_I164_L1.2400.99
136_G180_V1.2390.99
22_L219_L1.2270.99
110_D146_L1.2230.99
179_I200_T1.2120.99
3_K110_D1.1940.99
125_R173_D1.1910.99
10_I14_S1.1840.99
118_E167_D1.1790.99
82_V180_V1.1740.99
33_I216_I1.1720.99
70_R74_R1.1720.99
48_G54_Y1.1560.99
4_V39_D1.1490.99
92_I95_K1.1470.99
34_S78_P1.1430.99
19_I211_G1.1400.99
90_Y114_L1.1280.99
186_V208_Y1.1170.99
54_Y74_R1.1080.99
54_Y59_N1.1050.98
122_D125_R1.1040.98
181_I184_T1.1040.98
98_V126_A1.1030.98
83_S109_S1.1020.98
82_V135_M1.0990.98
76_N81_I1.0980.98
20_T252_K1.0970.98
216_I220_L1.0950.98
245_N249_I1.0930.98
4_V8_D1.0880.98
179_I198_V1.0820.98
200_T209_C1.0810.98
128_V176_V1.0810.98
241_S245_N1.0750.98
96_S100_N1.0690.98
193_T209_C1.0680.98
7_R152_R1.0660.98
220_L236_L1.0640.98
94_V126_A1.0610.98
155_G192_I1.0540.98
34_S77_P1.0390.98
183_N263_K1.0370.98
82_V111_A1.0350.98
93_D96_S1.0070.97
40_S82_V1.0040.97
186_V207_P1.0020.97
258_E262_Y0.9980.97
119_E163_Q0.9970.97
47_L68_H0.9870.97
54_Y64_D0.9840.97
8_D11_K0.9760.97
140_L183_N0.9760.97
189_A209_C0.9750.97
4_V78_P0.9750.97
20_T38_L0.9720.97
98_V127_I0.9620.96
85_M89_S0.9610.96
161_E188_I0.9610.96
121_K167_D0.9600.96
103_L107_A0.9560.96
166_K170_E0.9470.96
48_G64_D0.9450.96
167_D170_E0.9450.96
28_P222_L0.9420.96
63_R66_I0.9410.96
51_V217_H0.9410.96
64_D67_S0.9390.96
28_P51_V0.9270.95
246_Q249_I0.9250.95
119_E129_K0.9250.95
246_Q250_D0.9240.95
32_I244_I0.9170.95
193_T210_D0.9160.95
121_K170_E0.9110.95
59_N106_K0.9040.95
95_K99_K0.9030.95
160_E163_Q0.8990.95
145_F150_G0.8980.95
90_Y97_A0.8940.95
181_I192_I0.8930.94
207_P254_N0.8860.94
191_R194_E0.8840.94
98_V130_A0.8840.94
148_L183_N0.8820.94
80_L111_A0.8820.94
120_I123_I0.8760.94
22_L33_I0.8710.94
105_V133_P0.8700.94
128_V177_F0.8690.94
22_L38_L0.8680.94
237_K241_S0.8670.94
62_M65_M0.8660.94
19_I39_D0.8660.94
91_E106_K0.8610.93
180_V198_V0.8580.93
73_A76_N0.8540.93
47_L60_V0.8520.93
41_I81_I0.8510.93
66_I103_L0.8420.93
236_L240_I0.8410.93
165_I195_K0.8410.93
242_S245_N0.8380.92
162_E191_R0.8370.92
165_I169_L0.8340.92
28_P217_H0.8330.92
39_D252_K0.8320.92
31_K35_N0.8260.92
224_E237_K0.8230.92
155_G164_L0.8230.92
118_E170_E0.8160.91
233_Y243_A0.8140.91
70_R73_A0.8110.91
214_L229_F0.8080.91
52_L64_D0.8040.91
235_N239_I0.8030.91
185_Y205_A0.7930.90
118_E163_Q0.7920.90
153_T228_Y0.7920.90
117_G121_K0.7900.90
81_I110_D0.7890.90
94_V122_D0.7870.90
67_S71_A0.7850.90
236_L239_I0.7850.90
29_T217_H0.7840.90
111_A135_M0.7820.90
47_L52_L0.7800.89
9_F177_F0.7750.89
61_T64_D0.7740.89
234_V243_A0.7720.89
153_T263_K0.7690.89
89_S106_K0.7680.89
171_L179_I0.7650.88
183_N228_Y0.7640.88
98_V102_G0.7620.88
12_K252_K0.7610.88
34_S76_N0.7580.88
36_T241_S0.7570.88
4_V9_F0.7560.88
22_L244_I0.7550.88
66_I107_A0.7530.88
216_I244_I0.7500.87
73_A110_D0.7430.87
32_I237_K0.7420.87
20_T39_D0.7420.87
229_F261_H0.7350.86
39_D211_G0.7340.86
19_I40_S0.7310.86
27_Y31_K0.7300.86
250_D253_T0.7290.86
176_V179_I0.7250.86
233_Y255_K0.7230.86
65_M83_S0.7160.85
194_E208_Y0.7150.85
185_Y188_I0.7120.85
62_M66_I0.7090.84
91_E97_A0.7070.84
161_E191_R0.7040.84
95_K126_A0.6990.84
170_E173_D0.6930.83
29_T240_I0.6930.83
123_I126_A0.6930.83
136_G179_I0.6920.83
148_L154_I0.6910.83
22_L216_I0.6830.82
169_L173_D0.6830.82
73_A107_A0.6820.82
66_I69_T0.6820.82
102_G106_K0.6780.82
256_F263_K0.6750.81
31_K237_K0.6740.81
93_D123_I0.6740.81
186_V259_K0.6730.81
150_G154_I0.6730.81
140_L151_F0.6700.81
47_L67_S0.6690.81
12_K39_D0.6680.81
88_L98_V0.6660.81
55_T59_N0.6620.80
10_I197_N0.6610.80
118_E121_K0.6580.80
138_I179_I0.6560.80
58_L106_K0.6560.80
166_K169_L0.6550.79
170_E174_A0.6550.79
231_K255_K0.6520.79
217_H222_L0.6510.79
181_I200_T0.6470.79
94_V97_A0.6460.79
49_M227_P0.6460.79
221_G226_T0.6460.79
153_T183_N0.6440.78
112_V134_V0.6420.78
48_G61_T0.6410.78
90_Y100_N0.6410.78
227_P230_A0.6390.78
123_I127_I0.6370.78
6_I177_F0.6310.77
231_K258_E0.6300.77
230_A263_K0.6280.77
30_A76_N0.6280.77
70_R107_A0.6270.76
25_Y51_V0.6250.76
85_M90_Y0.6250.76
40_S80_L0.6230.76
128_V147_R0.6220.76
114_L127_I0.6220.76
21_M30_A0.6210.76
26_D29_T0.6160.75
196_L200_T0.6090.74
66_I70_R0.6090.74
183_N223_S0.6050.74
5_T140_L0.6040.74
116_G167_D0.6040.74
192_I195_K0.6030.74
6_I10_I0.6010.73
11_K14_S0.6010.73
29_T220_L0.6000.73
121_K174_A0.6000.73
161_E185_Y0.5990.73
102_G132_I0.5980.73
58_L144_R0.5940.73
40_S178_S0.5940.73
221_G232_S0.5930.72
259_K262_Y0.5930.72
164_L188_I0.5920.72
73_A77_P0.5920.72
30_A41_I0.5910.72
85_M104_L0.5910.72
147_R177_F0.5900.72
240_I244_I0.5870.72
151_F227_P0.5840.71
94_V98_V0.5840.71
114_L120_I0.5830.71
180_V196_L0.5830.71
100_N104_L0.5820.71
72_V81_I0.5810.71
151_F183_N0.5810.71
51_V71_A0.5780.70
122_D129_K0.5780.70
19_I201_I0.5760.70
223_S226_T0.5740.70
181_I189_A0.5740.70
138_I155_G0.5720.70
36_T245_N0.5690.69
8_D12_K0.5680.69
128_V174_A0.5660.69
201_I211_G0.5660.69
169_L197_N0.5640.69
82_V178_S0.5640.69
9_F12_K0.5630.68
141_T144_R0.5610.68
101_A132_I0.5610.68
131_G147_R0.5590.68
27_Y75_A0.5590.68
117_G123_I0.5550.67
233_Y236_L0.5530.67
203_I213_V0.5510.67
87_F131_G0.5500.67
184_T200_T0.5490.67
58_L145_F0.5470.66
219_L230_A0.5450.66
200_T210_D0.5420.66
105_V127_I0.5390.65
24_A43_V0.5390.65
8_D49_M0.5390.65
145_F151_F0.5380.65
24_A33_I0.5340.64
154_I263_K0.5320.64
12_K15_M0.5310.64
127_I130_A0.5310.64
35_N245_N0.5280.64
9_F19_I0.5260.63
124_I136_G0.5250.63
21_M82_V0.5240.63
19_I199_P0.5200.62
154_I185_Y0.5190.62
193_T208_Y0.5170.62
98_V123_I0.5170.62
26_D217_H0.5170.62
35_N237_K0.5170.62
124_I176_V0.5160.62
184_T188_I0.5160.62
24_A30_A0.5150.62
24_A41_I0.5140.62
60_V64_D0.5130.61
250_D261_H0.5120.61
216_I222_L0.5110.61
62_M103_L0.5100.61
43_V83_S0.5100.61
165_I192_I0.5080.61
31_K224_E0.5070.60
58_L149_G0.5060.60
105_V108_G0.5060.60
244_I248_I0.5060.60
153_T185_Y0.5050.60
249_I255_K0.5040.60
118_E129_K0.5040.60
4_V79_Q0.5040.60
164_L192_I0.5040.60
117_G180_V0.5000.59
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1o66A 3 0.9326 100 0.219 Contact Map
1m3uA 4 0.9775 100 0.227 Contact Map
1oy0A 4 0.9213 100 0.239 Contact Map
3vavA 4 0.9813 100 0.248 Contact Map
4lsbA 2 0.8989 100 0.542 Contact Map
4mg4A 4 0.8839 100 0.603 Contact Map
3lyeA 4 0.8989 100 0.618 Contact Map
2qiwA 2 0.8764 100 0.624 Contact Map
3eooA 4 0.9026 100 0.631 Contact Map
1xg4A 4 0.9101 100 0.634 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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